AbstractNanostructures are a viable candidate for the construction of simple blood sugar monitoring devices. Electrochemical oxidation based on the immobilization of glucose oxidase (GOx) on carbon nanostructures has paved the way for a modern approach to the determination of glucose levels in blood. Carbon nanotubes (CNTs) exhibit excellent electrical properties, resulting in increased interest in glucose biosensors based on CNTs. Its large surface area and optimum aspect ratio increase the total amount of immobilized biomaterials onto its surface. In this contribution, recent advances in the development of reliable methods to improve the electron-transfer mechanism of GOx in CNT-based glucose biosensors are highlighted. Moreover, mass production and growth mechanism of purified CNTs by chemical vapor deposition were discussed by emphasizing its growth-control aspects.