Boron neutron capture therapy induces cell cycle arrest and cell apoptosis of glioma stem/progenitor cells in vitro

The quality manajement system program of in vitro / in vivo test facility of Boron Neutron Capture Therapy (BNCT) methode as quality assurance requirement for utilization of radial pearcing beamport of Kartini research have been done. Identification and management of technical specification and parameters meassurement of to the radial piercing beamport have been determined for preparing in vitro / in vivo test facility. The parameters are epithermal neutron flux is 9,8243E+05 n cm-2 s-1and thermal neutron flux is 3,0691E+06 n cm-2 s-1, radiation shielding of parafin, dimension and size of piercing radial and instrumentatin and control system for automatic transfer of in vitro / in vivo samplels have been documented. Management system of the documents for fullfil basic guidance to perform working job of in vitro / in vivo at the piercing radial beamport of Kartini Research Reactor in order purpose utilization of the reactor for safety worker of the radiation area, society and invironment beeing safely

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Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer

Frontiers in Oncology ◽

10.3389/fonc.2021.614458 ◽

2021 ◽

Vol 11 ◽

Author(s):

Lixia Cao ◽

Shaorong Zhao ◽

Qianxi Yang ◽

Zhendong Shi ◽

Jingjing Liu ◽

...

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Cell Cycle Arrest ◽

Cell Apoptosis ◽

Combined Treatment ◽

Tunel Staining ◽

Cell Cycle Distribution ◽

Cycle Arrest

The multidrug-resistant (MDR) phenotype is usually accompanied by an abnormal expression of histone deacetylase (HDAC). Given that HDAC is vital in chromatin remodeling and epigenetics, inhibiting the role of HDAC has become an important approach for tumor treatment. However, the effect of HDAC inhibitors on MDR breast cancer has not been elucidated. This study aim to demonstrate the potential of chidamide (CHI) combined with the chemotherapy drug doxorubicin (DOX) to overcome chemotherapeutic resistance of breast cancer in vitro and in vivo, laying the experimental foundation for the next clinical application. The results showed that, CHI combined with DOX showed significant cytotoxicity to MDR breast cancer cells in vitro and in vivo compared with the CHI monotherapy. The cell cycle distribution results showed that CHI caused G0/G1 cell cycle arrest and inhibited cell growth regardless of the addition of DOX. At the same time, annexin V staining and TUNEL staining results showed that CHI enhanced the number of cell apoptosis in drug-resistant cells. The western blot analysis found that p53 was activated in the CHI-treated group and combined treatment group, and then the activated p53 up-regulated p21, apoptosis regulator recombinant protein (Puma), and pro-apoptotic protein Bax, down-regulated the apoptotic proteins Bcl-xL and Bcl-2, and activated the caspase cascade to induce apoptosis.

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Lithium increases proliferation of hippocampal neural stem/progenitor cells and rescues irradiation-induced cell cycle arrest in vitro

Oncotarget ◽

10.18632/oncotarget.5191 ◽

2015 ◽

Vol 6 (35) ◽

pp. 37083-37097 ◽

Cited By ~ 19

Author(s):

Giulia Zanni ◽

Elena Di Martino ◽

Anna Omelyanenko ◽

Michael Andäng ◽

Ulla Delle ◽

...

Keyword(s):

Cell Cycle ◽

Cell Cycle Arrest ◽

Progenitor Cells ◽

Cycle Arrest ◽

Neural Stem Progenitor Cells

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Cyclic-RGDyC functionalized liposomes for dual-targeting of tumor vasculature and cancer cells in glioblastoma: An in vitro boron neutron capture therapy study

Oncotarget ◽

10.18632/oncotarget.16625 ◽

2017 ◽

Vol 8 (22) ◽

pp. 36614-36627 ◽

Cited By ~ 20

Author(s):

Weirong Kang ◽

Darren Svirskis ◽

Vijayalekshmi Sarojini ◽

Ailsa L. McGregor ◽

Joseph Bevitt ◽

...

Keyword(s):

Cancer Cells ◽

Boron Neutron Capture Therapy ◽

Neutron Capture ◽

Tumor Vasculature ◽

Neutron Capture Therapy ◽

Boron Neutron Capture ◽

Dual Targeting

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Functionalized mesoporous silica nanoparticles for innovative boron-neutron capture therapy of resistant cancers

10.1101/471128 ◽

2018 ◽

Cited By ~ 1

Author(s):

Guillaume Vares ◽

Vincent Jallet ◽

Yoshitaka Matsumoto ◽

Cedric Rentier ◽

Kentaro Takayama ◽

...

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Boron Neutron Capture Therapy ◽

Neutron Capture ◽

Mesoporous Silica Nanoparticles ◽

Alpha Particles ◽

Neutron Capture Therapy ◽

Boron Neutron Capture

AbstractTreatment resistance, relapse and metastasis remain critical issues in some challenging cancers, such as chondrosarcomas. Boron-neutron Capture Therapy (BNCT) is a targeted radiation therapy modality that relies on the ability of boron atoms to capture low energy neutrons, yielding high linear energy transfer alpha particles. We have developed an innovative boron-delivery system for BNCT, composed of multifunctional fluorescent mesoporous silica nanoparticles (B-MSNs), grafted with an activatable cell penetrating peptide (ACPP) for improved penetration in tumors and with Gadolinium for magnetic resonance imaging (MRI)in vivo. Chondrosarcoma cells were exposedin vitroto an epithermal neutron beam after B-MSNs administration. BNCT beam exposure successfully induced DNA damage and cell death, including in radio-resistant ALDH+ cancer stem cells (CSCs), suggesting that BNCT using this system might be a suitable treatment modality for chondrosarcoma or other hard-to-treat cancers.

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