Demonstration of a Flexible Graphene-Based Biosensor for Sensitive and Rapid Detection of Ovarian Cancer Cells

AbstractIt is significant to develop an efficient early detection and prediction method for ovarian cancer via a facile and low-cost approach. To address such issues, herein, we develop a novel circulating tumor cell (CTC) detection method to sensitively detect ovarian cancer by using a flexible graphene-based biosensor on polyethylene terephthalate (PET) substrate. The results show that the graphene-based flexible biosensor demonstrates sensitive and rapid detection for ovarian cancer cells: it delivers obvious different responses for cell culture medium and cancer solution, different cancer cells and cancer cell solution with different concentrations; it demonstrates high sensitivity for detecting several tens of ovarian cancer cells per ml; moreover, the flexible graphene biosensor is very suitable for rapid and sensitive detection of ovarian cancer cells within 5 s. This work provides a low-cost and facile graphene biosensor fabrication strategy to sensitively and rapidly detect / identify CTC ovarian cancer cells. Graphical Abstract

Download Full-text

Graphene-Based Biosensors with High Sensitivity for Detection of Ovarian Cancer Cells

Molecules ◽

10.3390/molecules26237265 ◽

2021 ◽

Vol 26 (23) ◽

pp. 7265

Author(s):

Qi Wan ◽

Ling Han ◽

Yunchuan Guo ◽

Huijun Yu ◽

Li Tan ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Detection Method ◽

High Sensitivity ◽

Circulating Tumor Cell ◽

Electrical Signal ◽

Ovarian Cancer Cells ◽

Before And After ◽

Chip Fabrication ◽

A Cell

Ovarian cancer has the highest mortality rate in the world. Therefore, it is urgent but still challenging to develop an efficient circulating tumor cell (CTC) detection method to sensitively detect ovarian cancer. To address such issues, herein, for the first time, we present a novel CTC detection method for ovarian cancer cells by designing sensitive and rapid graphene-based biosensors. This graphene-based sensor, consisting of a cell pool and two electrodes, can be prepared by a conventional chip fabrication process. It demonstrates high-sensitivity detection even for several ovarian cancer cells by comparing the electrical signal before and after adding cell solution. Moreover, the graphene-based biosensors can perform rapid detection with good repeatability. This suggests that this novel method is possible to use for the early detection of ovarian cancer with very low CTC cell concentration. This work provides a novel and quick strategy to detect ovarian cancer and further judge or predict the risk of the transfer of ovarian cancer.

Download Full-text

Effects of nano-taxol/curcumin on ovarian cancer cells

Materials Express ◽

10.1166/mex.2020.1818 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1615-1619

Author(s):

Shuai Zhang ◽

Junhui Liang ◽

Changzhong Li ◽

Fei Wang

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Culture Medium ◽

Morphological Characteristics ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Pharmacodynamic Effect ◽

Skov3 Cells ◽

Inhibit Cell Proliferation

To investigate the pharmacodynamic effect of urushin nanoparticles upon the proliferation inhibition in human ovarian cancer SKOV3 cells, and in order to explore their biomechanism, the cell cycle and the percentage of apoptotic cells in human ovarian cancer SKOV3 cells were analyzed utilizing flow cytometry. The concentration of astragalin nanoparticles in SKOV3 cells was identified utilizing HPIC. Consequently, the morphological characteristics of SKOV3 cells in a culture medium of 5 mg/L were investigated and measured. In our findings, the 50 mg cancer cells containing 50 mg IC did not display this noted effect. The results exhibit the discovery that urushin nanoparticles inhibit cell proliferation, which is related to the inhibition of DNA replication and the regulation of the cell proliferation cycle. HPLC results demonstrated that the pharmacological effect of urushin nanoparticles was directly related to the drug concentration present within the studied cells. Hence, urushin nanoparticles can effectively enter cells and then effectively inhibit cell proliferation.

Download Full-text