Neotenic phenomenon in gene expression in the skin of Foxn1- deficient (nude) mice - a projection for regenerative skin wound healing

Skin wound healing is a complex and dynamic process that aims to restore lesioned tissues. Collagen-based skin substitutes are a promising treatment to promote wound healing by mimicking the native skin structure. Recently, collagen from marine organisms has gained interest as a source for producing biomaterials for skin regenerative strategies. This preliminary study aimed to describe the application of a collagen-based skin-like scaffold (CBSS), manufactured with collagen extracted from sea urchin food waste, to treat experimental skin wounds in a large animal. The wound-healing process was assessed over different time points by the means of clinical, histopathological, and molecular analysis. The CBSS treatment improved wound re-epithelialization along with cell proliferation, gene expression of growth factors (VEGF-A), and development of skin adnexa throughout the healing process. Furthermore, it regulated the gene expression of collagen type I and III, thus enhancing the maturation of the granulation tissue into a mature dermis without any signs of scarring as observed in untreated wounds. The observed results (reduced inflammation, better re-epithelialization, proper development of mature dermis and skin adnexa) suggest that sea urchin-derived CBSS is a promising biomaterial for skin wound healing in a “blue biotechnologies” perspective for animals of Veterinary interest.

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From Inflammation to Cutaneous Repair: Topical Application of Lupeol Improves Skin Wound Healing in Rats by Modulating the Cytokine Levels, NF-κB, Ki-67, Growth Factor Expression, and Distribution of Collagen Fibers

International Journal of Molecular Sciences ◽

10.3390/ijms21144952 ◽

2020 ◽

Vol 21 (14) ◽

pp. 4952 ◽

Cited By ~ 1

Author(s):

Fernando Pereira Beserra ◽

Lucas Fernando Sérgio Gushiken ◽

Ana Júlia Vieira ◽

Danilo Augusto Bérgamo ◽

Patrícia Luísa Bérgamo ◽

...

Keyword(s):

Gene Expression ◽

Wound Healing ◽

Growth Factor ◽

Skin Wound ◽

Collagen Fibers ◽

Skin Wound Healing ◽

Ki 67 ◽

Healing Effect ◽

Cytokine Levels ◽

Wound Healing Effect

Skin wound healing is a highly complex event that involves different mediators at the cellular and molecular level. Lupeol has been reported to possess different biological activities, such as anti-inflammatory, antioxidant, antidiabetic, and in vitro wound healing properties, which motivated us to proceed with in vivo studies. We aimed to investigate the wound healing effect of lupeol-based cream for 3, 7, and 14 days. Wound excisions were induced on the thoraco-lumbar region of rats and topically treated immediately after injury induction. Macroscopic, histopathological, and immunohistochemical analyses were performed. Cytokine levels were measured by ELISA and gene expression was evaluated by real-time RT-qPCR. Our results showed a strong wound-healing effect of lupeol-based cream after 7 and 14 days. Lupeol treatment caused a reduction in proinflammatory cytokines (TNF-a, IL-1β, and IL-6) and gene and protein NF-κB expression, and positively altered IL-10 levels, showing anti-inflammatory effects in the three treatment periods. Lupeol treatment showed involvement in the proliferative phase by stimulating the formation of new blood vessels, increasing the immunostaining of Ki-67 and gene expression, and immunolabeling of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF), and increasing gene expression of transforming growth factor beta-1 (TGF-β1) after seven days of treatment. Lupeol was also involved in the tissue regeneration phase by increasing the synthesis of collagen fibers noted in the three treatment periods analyzed. Our findings suggest that lupeol may serve as a novel therapeutic option to treat cutaneous wounds by regulating mechanisms involved in the inflammatory, proliferative, and tissue-remodeling phases.

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Dexpanthenol Modulates Gene Expression in Skin Wound Healing in vivo

Skin Pharmacology and Physiology ◽

10.1159/000341144 ◽

2012 ◽

Vol 25 (5) ◽

pp. 241-248 ◽

Cited By ~ 31

Author(s):

R. Heise ◽

C. Skazik ◽

Y. Marquardt ◽

K. Czaja ◽

K. Sebastian ◽

...

Keyword(s):

Gene Expression ◽

Wound Healing ◽

Skin Wound ◽

Skin Wound Healing

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Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice

Stem Cell Research & Therapy ◽

10.1186/s13287-015-0238-3 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 31

Author(s):

Jonathan Rodriguez ◽

Fabien Boucher ◽

Charlotte Lequeux ◽

Audrey Josset-Lamaugarny ◽

Ondine Rouyer ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Nude Mice ◽

Skin Wound ◽

Intradermal Injection ◽

Adipose Derived Stem Cells ◽

Skin Wound Healing

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Transcriptomic Analysis of Human Skin Wound Healing and Rejuvenation Following Ablative Fractional Laser Treatment

10.1101/2021.07.26.453869 ◽

2021 ◽

Author(s):

Joseph D Sherrill ◽

Deborah Finlay ◽

Robert L Binder ◽

Michael K Robinson ◽

Xingtao Wei ◽

...

Keyword(s):

Gene Expression ◽

Wound Healing ◽

Laser Treatment ◽

Skin Wound ◽

Fractional Laser ◽

Skin Rejuvenation ◽

Skin Wound Healing ◽

Age Related ◽

Ablative Fractional Laser ◽

Laser Treatments

Ablative fractional laser treatment is considered the gold standard for skin rejuvenation. In order to understand how fractional laser works to rejuvenate skin, we performed microarray profiling on skin biopsies to identify temporal and dose-response changes in gene expression following fractional laser treatment. The backs of 14 women were treated with ablative fractional laser (Fraxel) and 4 mm punch biopsies were collected from an untreated site and at the treated sites 1, 3, 7, 14, 21 and 28 days after the single treatment. In addition, in order to understand the effect that multiple fractional laser treatments have on skin rejuvenation, several sites were treated sequentially with either 1, 2, 3, or 4 treatments (with 28 days between treatments) followed by the collection of 4 mm punch biopsies. RNA was extracted from the biopsies, analyzed using Affymetrix U219 chips and gene expression was compared between untreated and treated sites. We observed dramatic changes in gene expression as early as 1 day after fractional laser treatment with changes remaining elevated even after 1 month. Analysis of individual genes demonstrated significant and time related changes in inflammatory, epidermal, and dermal genes, with dermal genes linked to extracellular matrix formation changing at later time points following fractional laser treatment. When comparing the age-related changes in skin gene expression to those induced by fractional laser, it was observed that fractional laser treatment reverses many of the changes in the aging gene expression. Finally, multiple fractional laser treatments resulted in continued changes in gene expression, with many genes either differentially regulated or continuously upregulated with increasing number of treatments, indicating that maximal skin rejuvenation requires multiple fractional laser treatments. In conclusion, fractional laser treatment of skin activates several biological processes involved in wound healing and tissue regeneration, all of which significantly contribute to the rejuvenating effect of fractional laser treatment on aged skin.

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