Differences between fast and slow muscles in scallops revealed through proteomics and transcriptomics

To investigate the plasticity of slow and fast muscles undergoing slow-to-fast transition, rat soleus (SOL), gastrocnemius (GAS), and extensor digitorum longus (EDL) muscles were exposed for 14 days to 1) unweighting by hindlimb suspension (HU), or 2) treatment with the β2-adrenergic agonist clenbuterol (CB), or 3) a combination of both (HU-CB). In general, HU elicited atrophy, CB induced hypertrophy, and HU-CB partially counteracted the HU-induced atrophy. Analyses of myosin heavy (MHC) and light chain (MLC) isoforms revealed HU- and CB-induced slow-to-fast transitions in SOL (increases of MHCIIa with small amounts of MHCIId and MHCIIb) and the upregulation of the slow MHCIa isoform. The HU- and CB-induced changes in GAS consisted of increases in MHCIId and MHCIIb (“fast-to-faster transitions”). Changes in the MLC composition of SOL and GAS consisted of slow-to-fast transitions and mainly encompassed an exchange of MLC1s with MLC1f. In addition, MLC3f was elevated whenever MHCIId and MHCIIb isoforms were increased. Because the EDL is predominantly composed of type IID and IIB fibers, HU, CB, and HU-CB had no significant effect on the MHC and MLC patterns.

Download Full-text

Expression of the myogenic regulatory factors and the sarcoplasmic/endoplasmic reticulum Ca2+ ATPases in regenerating fast and slow muscles of the rat

Neuromuscular Disorders ◽

10.1016/s0960-8966(97)87356-7 ◽

1997 ◽

Vol 7 (6-7) ◽

pp. 475

Author(s):

Erno Zador ◽

Luca Mendler ◽

Gerda Szakonyi ◽

Gabor Racz ◽

Aniko Gorbe ◽

...

Keyword(s):

Endoplasmic Reticulum ◽

Myogenic Regulatory Factors ◽

Regulatory Factors ◽

Fast And Slow Muscles

Download Full-text

Pharmacological and physiological study of motor dysfunction in spontaneous thymoma Buffalo/Mna rats: comparison between fast and slow muscles

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)54677-7 ◽

1981 ◽

Vol 31 ◽

pp. 212

Author(s):

Fuminori Kato ◽

Minoru Watanabe

Keyword(s):

Motor Dysfunction ◽

Physiological Study ◽

Fast And Slow Muscles

Download Full-text

Characterization and Functional Analysis of Polyadenylation Sites in Fast and Slow Muscles

BioMed Research International ◽

10.1155/2020/2626584 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Lulu Deng ◽

Long Li ◽

Cheng Zou ◽

Chengchi Fang ◽

Changchun Li

Keyword(s):

Single Molecule ◽

Posttranscriptional Regulation ◽

Muscle Development ◽

Target Genes ◽

Rna Binding ◽

De Novo ◽

Rna Binding Proteins ◽

Fast And Slow Muscles ◽

Molecular Features ◽

Polyadenylation Sites

Many increasing documents have proved that alternative polyadenylation (APA) events with different polyadenylation sites (PAS) contribute to posttranscriptional regulation. However, little is known about the detailed molecular features of PASs and its role in porcine fast and slow skeletal muscles through microRNAs (miRNAs) and RNA binding proteins (RBPs). In this study, we combined single-molecule real-time sequencing and Illumina RNA-seq datasets to comprehensively analyze polyadenylation in pigs. We identified a total of 10,334 PASs, of which 8734 were characterized by reference genome annotation. 32.86% of PAS-associated genes were determined to have more than one PAS. Further analysis demonstrated that tissue-specific PASs between fast and slow muscles were enriched in skeletal muscle development pathways. In addition, we obtained 1407 target genes regulated by APA events through potential binding 69 miRNAs and 28 RBPs in variable 3′ UTR regions and some are involved in myofiber transformation. Furthermore, the de novo motif search confirmed that the most common usage of canonical motif AAUAAA and three types of PASs may be related to the strength of motifs. In summary, our results provide a useful annotation of PASs for pig transcriptome and suggest that APA may serve as a role in fast and slow muscle development under the regulation of miRNAs and RBPs.

Download Full-text