scholarly journals Comparative genomics and physiological investigation supported safety, cold adaptation, efficient hydrolytic and plant growth-promoting potential of psychrotrophic Glutamicibacter arilaitensis LJH19, isolated from night-soil compost

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Shruti Sinai Borker ◽  
Aman Thakur ◽  
Sanjeet Kumar ◽  
Sareeka Kumari ◽  
Rakshak Kumar ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Plant Growth ◽  
Cold Adaptation ◽  
Gene Clusters ◽  
Type Iii Polyketide Synthase ◽  
Pgp Traits ◽  
Bacterial Consortia ◽  
Hydrolytic Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background Night-soil compost (NSC) has traditionally been conserving water and a source of organic manure in northwestern Himalaya. Lately, this traditional method is declining due to modernization, its unhygienic conditions, and social apprehensions. Reduction in the age-old traditional practice has led to excessive chemical fertilizers and water shortage in the eco-sensitive region. In the current study, a bacterium has been analyzed for its safety, cold-adaptation, efficient degradation, and plant growth-promoting (PGP) attributes for its possible application as a safe bioinoculant in psychrotrophic bacterial consortia for improved night-soil composting. Results Glutamicibacter arilaitensis LJH19, a psychrotrophic bacterium, was isolated from the NSC of Lahaul valley in northwestern Himalaya. The strain exhibited amylase (186.76 ± 19.28 U/mg), cellulase (21.85 ± 0.7 U/mg), and xylanase (11.31 ± 0.51 U/mg) activities at 10 °C. Possessing efficient hydrolytic activities at low-temperature garners the capability of efficient composting to LJH19. Additionally, the strain possessed multiple PGP traits such as indole acetic acid production (166.11 ± 5.7 μg/ml), siderophore production (85.72 ± 1.06% psu), and phosphate solubilization (44.76 ± 1.5 μg/ml). Enhanced germination index and germination rate of pea seeds under the LJH19 inoculation further supported the bacterium’s PGP potential. Whole-genome sequencing (3,602,821 bps) and genome mining endorsed the cold adaptation, degradation of polysaccharides, and PGP traits of LJH19. Biosynthetic gene clusters for type III polyketide synthase (PKS), terpene, and siderophore supplemented the endorsement of LJH19 as a potential PGP bacterium. Comparative genomics within the genus revealed 217 unique genes specific to hydrolytic and PGP activity. Conclusion The physiological and genomic evidence promotes LJH19 as a potentially safe bio-inoculant to formulate psychrotrophic bacterial consortia for accelerated degradation and improved night-soil compost.

scholarly journals Comparative Genomics and Physiological Investigation Supported Safety, Cold Adaptation, Efficient Hydrolytic and Plant Growth-promoting Potential of Psychrotrophic Glutamicibacter Arilaitensis LJH19, Isolated from Night-Soil Compost

2020 ◽  
Author(s):  
Shruti Borker ◽  
Aman Thakur ◽  
Sanjeet Kumar ◽  
Sareeka Kumari ◽  
Rakshak Kumar ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Plant Growth ◽  
Cold Adaptation ◽  
Growth Potential ◽  
Type Iii Polyketide Synthase ◽  
Pgp Traits ◽  
Bacterial Consortia ◽  
Hydrolytic Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background: Night-soil compost (NSC) has traditionally been conserving water and a source of organic manure in northwestern Himalaya. Lately, this traditional method is declining due to modernization, its unhygienic conditions, and social apprehensions. Reduction in the age-old traditional practice has led to excessive chemical fertilizers and water shortage in the eco-sensitive region. In the current study, a bacterium has been analysed for its safety, cold-adaptation, efficient degradation, and plant growth potential attributes for its possible application as a safe bioinoculant in psychrotrophic bacterial consortia for improved night-soil composting.Results: Glutamicibacter arilaitensis LJH19, a psychrotrophic bacterium, was isolated from the night-soil compost of Lahaul valley in northwestern Himalaya. The strain exhibited amylase (186.76 ± 19.28 U/mg), cellulase (21.85 ± 0.7 U/mg) and xylanase (11.31± 0.51 U/mg) activities at 10°C. Possessing efficient hydrolytic activities at low-temperature garners the capability of efficient composting to LJH19. Additionally, the strain possessed multiple plant growth-promoting (PGP) traits such as indole acetic acid production (166.11 ± 5.7 µg/ml), siderophore production (85.72 ±1.06 % psu), and phosphate solubilization (44.76 ± 1.5 µg/ml). Enhanced germination index and germination rate of pea seeds under the LJH19 inoculation further supported the bacterium's PGP potential. Whole-genome sequencing (3,602,821 bps) and genome mining endorsed the cold adaptation, degradation of polysaccharides and, plant growth-promoting traits of LJH19. Biosynthetic gene clusters for type III polyketide synthase (PKS), terpene, and siderophore supplemented the endorsement of LJH19 as a potential PGP bacterium. Comparative genomics within the genus revealed 217 unique genes specific to hydrolytic and PGP activity. Conclusion: The physiological and genomic evidence promotes LJH19 as a potentially safe bio-inoculant to formulate psychrotrophic bacterial consortia for accelerated degradation and improved night-soil compost.

scholarly journals Investigation of a night soil compost psychrotrophic bacterium Glutamicibacter arilaitensis LJH19 for its safety, efficient hydrolytic and plant growth-promoting potential

2020 ◽  
Author(s):  
Shruti Sinai Borker ◽  
Aman Thakur ◽  
Sanjeet Kumar ◽  
Sareeka Kumari ◽  
Rakshak Kumar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gene Clusters ◽  
Cold Climate ◽  
Whole Genome Sequence ◽  
Potential Candidate ◽  
Type Iii Polyketide Synthase ◽  
Pgp Traits ◽  
Hydrolytic Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractNight soil compost (NSC) has traditionally been a source of organic manure in north-western Himalaya. Lately, this traditional method is declining due to modernization, its unhygienic conditions and social apprehensions. Reduction in the age-old traditional practice has led to excessive usage of chemical fertilizers and shortage of water in the eco-sensitive region. Microbiological intervention was attempted to obtain bacterial consortia for accelerated degradation of human faeces in cold climate to improvise this traditional knowledge. Glutamicibacter arilaitensis LJH19, a psychrotrophic bacteria was identified as one such potential candidate for the proposed consortia. The bacterium was isolated from NSC of Lahaul valley and exhibited potential hydrolytic activities, the specific activities of amylase, cellulase and xylanase was observed as 186.76 U/mg, 21.85 U/mg and 11.31 U/mg respectively. Additionally, the strain possessed multiple plant growth-promoting (PGP) traits. The bacterium produced 166.11 µg/ml indole acetic acid and 85.72 % siderophore units, and solubilized 44.76 µg/ml phosphate. Whole genome sequence (3,602,821 bps) endorsed the cold adaptation, polysaccharide metabolism, PGP potential of the bacterium. Genome mining revealed biosynthetic gene clusters for type III polyketide synthase (PKS), terpene, and siderophore in agreement with its potential PGP traits. Comparative genomics within the genus revealed 217 unique genes specific to hydrolytic and PGP activity. Negative haemolysis and biofilm production and susceptibility towards all 12 tested antibiotics indicated the bacterium to be a safe bioinoculant. Genomic investigation supported the bacterium safety with absence of any virulence and antibiotic resistance genes. We propose the strain LJH19 to be a potentially safe bioinoculant candidate for efficient degradation of night soil owing to its survivability in cold and its efficient hydrolytic and PGP potential.

scholarly journals Endophytic Burkholderia sp. SSG as a potential biofertilizer promoting boxwood growth

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9547
Author(s):  
Ping Kong ◽  
Chuanxue Hong
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Plant Protection ◽  
Growth Promotion ◽  
Agar Plate ◽  
Gene Clusters ◽  
Plant Weight ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background Burkholderia sp. SSG is a bacterial endophyte isolated from boxwood leaves showing a resistant response to infection by the boxwood blight pathogen Calonectria pseudonaviculata. SSG acted as a protective and curative biocontrol agent for boxwood blight and as a bio-sanitizer of disease inoculum in the field. Many gene clusters involved in antibiotic production and plant growth promotion (PGP) were found in the genome, giving this endophyte great application potential as a treatment for plant protection. However, the PGP features have not been documented. This study investigated the plant growth promotion activity of SSG in boxwood. Methods To determine whether SSG is a plant growth promoting bacterium, four PGP traits, auxin and siderophore production, nitrogen fixation and phosphate solubilization, were examined in the laboratory with colorimetric or agar plate assays. The plant growth promoting activity of SSG was tested on three boxwood varieties characterized by slow, intermediate and fast growth rates, namely Justin Brouwers, Buddy and Winter Gem, respectively. These plants were drenched with an SSG cell suspension or water and washed plant weight was compared before and after treatment to determine growth changes after 10 months. Results The SSG culture was sustainable on nitrogen free media, suggesting that SSG may fix atmospheric nitrogen. It was also a strong phosphate solubilizer and a potent siderophore and indole-3-acetic acid (IAA) producer. Significant growth promotion was observed on boxwood cultivars Justin Brouwers, Buddy and Winter Gem 10 months after plant roots were drenched with SSG cells. The growth rate of treated plants was 76.1, 58.3, and 37.3% higher than that of the control, respectively. The degree of growth promotion was significantly different among plant varieties, notably more pronounced with the slow and intermediate growers. This study demonstrates that the SSG bacterium has multiple PGP traits and is a prospective plant biofertilizer.

scholarly journals Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Cd Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

scholarly journals Multi-Trait Wheat Rhizobacteria from Calcareous Soil with Biocontrol Activity Promote Plant Growth and Mitigate Salinity Stress

2021 ◽  
Vol 9 (8) ◽  
pp. 1588
Author(s):  
Anastasia Venieraki ◽  
Styliani N. Chorianopoulou ◽  
Panagiotis Katinakis ◽  
Dimitris L. Bouranis
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Calcareous Soil ◽  
In Vitro Studies ◽  
Pgp Traits ◽  
Biocontrol Activity ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Microbial Fertilizers

Plant growth promoting rhizobacteria (PGPR) can be functional microbial fertilizers and/or biological control agents, contributing to an eco-spirit and safe solution for chemical replacement. Therefore, we have isolated rhizospheric arylsulfatase (ARS)-producing bacteria, belonging to Pseudomonas and Bacillus genus, from durum wheat crop grown on calcareous soil. These isolates harbouring plant growth promoting (PGP) traits were further evaluated in vitro for additional PGP traits, including indole compounds production and biocontrol activity against phytopathogens, limiting the group of multi-trait strains to eight. The selected bacterial strains were further evaluated for PGP attributes associated with biofilm formation, compatibility, salt tolerance ability and effect on plant growth. In vitro studies demonstrated that the multi-trait isolates, Bacillus (1.SG.7, 5.SG.3) and Pseudomonas (2.SG.20, 2.C.19) strains, enhanced the lateral roots abundance and shoots biomass, mitigated salinity stress, suggesting the utility of beneficial ARS-producing bacteria as potential microbial fertilizers. Furthermore, in vitro studies demonstrated that compatible combinations of multi-trait isolates, Bacillus sp. 1.SG.7 in a mixture coupled with 5.SG.3, and 2.C.19 with 5.SG.3 belonging to Bacillus and Pseudomonas, respectively, may enhance plant growth as compared to single inoculants.

scholarly journals Efficacy of Plant Growth-Promoting Bacteria Bacillus cereus YN917 for Biocontrol of Rice Blast

2021 ◽  
Vol 12 ◽  
Author(s):  
Hu Zhou ◽  
Zuo-hua Ren ◽  
Xue Zu ◽  
Xi-yue Yu ◽  
Hua-jun Zhu ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Plant Growth ◽  
Bacillus Cereus ◽  
Rice Blast ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Plant Growth Promoting Bacteria ◽  
Detached Leaf ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus YN917, obtained from a rice leaf with remarkable antifungal activity against Magnaporthe oryzae, was reported in our previous study. The present study deciphered the possible biocontrol properties. YN917 strain exhibits multiple plant growth-promoting and disease prevention traits, including production of indole-3-acetic acid (IAA), ACC deaminase, siderophores, protease, amylase, cellulase, and β-1,3-glucanase, and harboring mineral phosphate decomposition activity. The effects of the strain YN917 on growth promotion and disease prevention were further evaluated under detached leaf and greenhouse conditions. The results revealed that B. cereus YN917 can promote seed germination and seedling plant growth. The growth status of rice plants was measured from the aspects of rice plumule, radicle lengths, plant height, stem width, root lengths, fresh weights, dry weights, and root activity when YN917 was used as inoculants. YN917 significantly reduced rice blast severity under detached leaf and greenhouse conditions. Genome analysis revealed the presence of gene clusters for biosynthesis of plant promotion and antifungal compounds, such as IAA, tryptophan, siderophores, and phenazine. In summary, YN917 can not only be used as biocontrol agents to minimize the use of chemical substances in rice blast control, but also can be developed as bio-fertilizers to promote the rice plant growth.

scholarly journals Characterization of a Versatile Plant Growth-Promoting Rhizobacterium Pseudomonas mediterranea Strain S58

2020 ◽  
Vol 8 (3) ◽  
pp. 334
Author(s):  
Yilin Gu ◽  
Jing Wang ◽  
Zhenyuan Xia ◽  
Hai-Lei Wei
Keyword(s):  
Cell Death ◽  
Plant Growth ◽  
Bacterial Genome ◽  
Gene Clusters ◽  
Marker Genes ◽  
Circular Chromosome ◽  
A Genome ◽  
Sharp Eyespot ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacterial strain S58 was isolated from the tobacco rhizosphere. It showed strong antagonism against a battery of plant pathogenic fungi and bacteria, and controlled wheat sharp eyespot and tobacco wildfire diseases efficiently. Further tests showed that strain S58 solubilized organic phosphate and produced siderophore, protease, ammonia, and indole-3-acetic acid. In Arabidopsis thaliana, it promoted plant growth and changed root system architecture by restricting the growth of primary roots and increasing lateral root numbers. We relied on morphological, biochemical, physiological characteristics, and molecular phylogenic analysis to identify strain S58 as Pseudomonas mediterranea. The complete genome of strain S58 has a single circular chromosome of 6,150,838 bp with a 61.06% G+C content. The bacterial genome contained 5,312 predicted genes with an average length of 992.90 bp. A genome analysis suggested that P. mediterranea S58 was a rich cyclic lipopeptide (CLP)-producing strain that possessed seven non-ribosomal peptide gene clusters for CLP synthesis. Leaf inoculation of the bacterial culture and supernatants triggered cell death-like immunity in tobacco. Quantitative real-time PCR assays showed that the strain S58 induced the expression of pattern-triggered immunity and cell death marker genes, but not jasmonic acid marker genes. The results suggested that P. mediterranea S58 is a novel, versatile plant growth-promoting agent with multiple beneficial traits for plants.

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