scholarly journals Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jiangqin Song ◽  
Xiaorong Hu ◽  
Xiaolong Li ◽  
Youping Chen ◽  
Xiangyuan Yan ◽  
...  
Keyword(s):  
Chronic Infection ◽  
Culture Medium ◽  
Q Fever ◽  
Brucella Melitensis ◽  
Gram Negative Bacteria ◽  
Left Forearm ◽  
Poor Appetite ◽  
Gram Negative ◽  
Brucella Ovis ◽  
Missed Diagnosis

AbstractRickettsia is the pathogen of Q fever, Brucella ovis is the pathogen of brucellosis, and both of them are Gram-negative bacteria which are parasitic in cells. The mixed infection of rickettsia and Brucella ovis is rarely reported in clinic. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Here, we report a case of co-infection Rickettsia burneti and Brucella melitensis. The patient is a 49-year-old sheepherder, who was hospitalized with left forearm trauma. Three days after admission, the patient developed fever of 39.0°C, accompanied by sweating, fatigue, poor appetite and headache. Indirect immunofluorescence (IFA) was used to detect Rickettsia burneti IgM. After 72 hours of blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. Patients were treated with doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for 4 weeks. After treatment, the symptoms disappeared quickly, and there was no sign of recurrence or chronic infection. Q fever and Brucella may exist in high-risk practitioners, so we should routinely detect these two pathogens to prevent missed diagnosis.

scholarly journals Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

2021 ◽  
Author(s):  
Jiangqin Song ◽  
Xiaolong Li ◽  
Xiaorong Hu ◽  
Yan Ding ◽  
Junyang Zhou ◽  
...  
Keyword(s):  
Causative Agent ◽  
Q Fever ◽  
Brucella Melitensis ◽  
Total Duration ◽  
Immunofluorescence Assay ◽  
Excessive Sweating ◽  
Case Presentation ◽  
Gram Negative ◽  
Missed Diagnosis ◽  
Loss Of Appetite

Abstract Background Rickettsia burneti is the causative agent of Q fever, Brucella melitensis is the causative agent of brucellosis, both of which are intracellular parasitic gram-negative bacteria. Rickettsia burneti and Brucella melitensis coinfection is fairly rarely reported in clinical. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Case Presentation Here, we report a case of Rickettsia burneti and Brucella melitensis co-infection. The patient is a 49-year-old sheepherder, was hospitalized for left forearm trauma. Three days after admission, the patient’s fever up to 39.0°C with excessive sweating, weakness, loss of appetite and headache. Rickettsia burneti IgM was detected positive by indirect immunofluorescence assay (IFA). After 72 hours blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. The patient accept therapy of doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for a total duration of four weeks. After receiving treatment, the patient’s symptoms disappeared rapidly, there has been no relapse or signs of chronic infection.Conclusion For high-risk practitioners, Q fever and brucellosis may be present in one patient, we should routinely test for both pathogens through a variety of tests to prevent missed diagnosis.

scholarly journals Antiadherent and Antibiofilm Activity ofHumulus lupulusL. Derived Products: New Pharmacological Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Marcin Rozalski ◽  
Bartlomiej Micota ◽  
Beata Sadowska ◽  
Anna Stochmal ◽  
Dariusz Jedrejek ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Culture Medium ◽  
Antimicrobial Properties ◽  
Antibiofilm Activity ◽  
Pharmacological Properties ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Abiotic Surfaces ◽  
First Time ◽  
Plant Compounds

New antimicrobial properties of products derived fromHumulus lupulusL. such as antiadherent and antibiofilm activities were evaluated. The growth of gram-positive but not gram-negative bacteria was inhibited to different extents by these compounds. An extract of hop cones containing 51% xanthohumol was slightly less active againstS. aureusstrains (MIC range 31.2–125.0 μg/mL) than pure xanthohumol (MIC range 15.6–62.5 μg/mL). The spent hop extract, free of xanthohumol, exhibited lower but still relevant activity (MIC range 1-2 mg/mL). There were positive coactions of hop cone, spent hop extracts, and xanthohumol with oxacillin against MSSA and with linezolid against MSSA and MRSA. Plant compounds in the culture medium at sub-MIC concentrations decreased the adhesion ofStaphylococcito abiotic surfaces, which in turn caused inhibition of biofilm formation. The rate of mature biofilm eradication by these products was significant. The spent hop extract at MIC reduced biofilm viability by 42.8%, the hop cone extract by 74.8%, and pure xanthohumol by 86.5%. When the hop cone extract or xanthohumol concentration was increased, almost complete biofilm eradication was achieved (97–99%). This study reveals the potent antibiofilm activity of hop-derived compounds for the first time.

scholarly journals Lipopolysaccharide modification in Gram-negative bacteria during chronic infection

2016 ◽  
Vol 40 (4) ◽  
pp. 480-493 ◽  
Author(s):  
Rita F. Maldonado ◽  
Isabel Sá-Correia ◽  
Miguel A. Valvano
Keyword(s):  
Chronic Infection ◽  
Gram Negative Bacteria ◽  
Gram Negative

scholarly journals The Ton System, an ABC Transporter, and a Universally Conserved GTPase Are Involved in Iron Utilization by Brucella melitensis 16M

2004 ◽  
Vol 72 (10) ◽  
pp. 5783-5790 ◽  
Author(s):  
Isabelle Danese ◽  
Valerie Haine ◽  
Rose-May Delrue ◽  
Anne Tibor ◽  
Pascal Lestrate ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Brucella Melitensis ◽  
Iron Acquisition ◽  
Regulatory Function ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Cellular Models ◽  
Iron Assimilation ◽  
Transposon Mutants ◽  
Iron Utilization

ABSTRACT Brucella spp. are gram-negative intracellular facultative pathogens that are known to produce 2,3-dihydroxybenzoic acid (DHBA), a catechol siderophore that is essential for full virulence in the natural host. The mechanism of DHBA entry into Brucella and other gram-negative bacteria is poorly understood. Using mini-Tn5Kmcat mutagenesis, we created a transposon library of Brucella melitensis 16M and isolated 32 mutants with a defect in iron acquisition or assimilation. Three of these transposon mutants are deficient in utilization of DHBA. Analysis of these three mutants indicated that the ExbB, DstC, and DugA proteins are required for optimal assimilation of DHBA and/or citrate. ExbB is part of the Ton complex, and DstC is a permease homologue of an iron(III) ABC transporter; in gram-negative bacteria these two complexes are involved in the uptake of iron through the outer and inner membranes, respectively. DugA is a new partner in iron utilization that exhibits homology with the bacterial conserved GTPase YchF. Based on this homology, DugA could have a putative regulatory function in iron assimilation in Brucella. None of the three mutants was attenuated in cellular models or in the mouse model of infection, which is consistent with the previous suggestion that DHBA utilization is not required in these models.

scholarly journals A Selective Culture Medium for Screening Ceftazidime-Avibactam Resistance in Enterobacterales and Pseudomonas aeruginosa

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Mustafa Sadek ◽  
Laurent Poirel ◽  
Camille Tinguely ◽  
Patrice Nordmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lower Limit ◽  
Culture Medium ◽  
Limit Of Detection ◽  
Resistance Mechanisms ◽  
Gram Negative Bacteria ◽  
Excellent Performance ◽  
Gram Negative ◽  
Content Type ◽  
Selective Culture Medium

ABSTRACT The SuperCAZ/AVI medium was developed for screening ceftazidime-avibactam (CZA) resistance among Gram-negative bacteria (Enterobacterales and Pseudomonas aeruginosa). It was evaluated using 50 CZA-susceptible and 42 CZA-resistant Gram-negative isolates. Its sensitivity and specificity of detection were 100%. Excellent performance of the medium was also observed by testing spiked stools, with the lower limit of detection ranging from 101 to 102 CFU/ml. This screening medium provides the opportunity to detect CZA-resistant isolates regardless of their resistance mechanisms.

Relationship between Adaptive Changing of Lysophosphatidylethanolamine Content in the Bacterial Envelope and Ampicillin Sensitivity of Yersinia pseudotuberculosis

2018 ◽  
Vol 28 (5) ◽  
pp. 236-239
Author(s):  
Nina Sanina ◽  
Lyudmila Pomazenkova ◽  
Svetlana Bakholdina ◽  
Natalia Chopenko ◽  
Anna Zabolotnaya ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Culture Medium ◽  
Bacterial Resistance ◽  
Yersinia Pseudotuberculosis ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Control Value ◽  
And Function

The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in <i>Yersinia pseudotuberculosis</i> induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content affect the resistance of bacteria to ampicillin. The addition of glucose to the culture medium was shown to simultaneously increase the level of LPE and minimum inhibitory concentration (MIC) for ampicillin of <i>Y. pseudotuberculosis</i> cells 6- and 2-fold, respectively. However, the coadministration of glucose and polyphenol extract from buckwheat husks reduced the content of LPE 2-fold and restored MIC to the control value. Thus, PBEH can be used as antibiotic adjuvant to improve an antibiotic’s ability to cross the outer membrane. The present work demonstrated: (i) the role of adaptive changes in the lipid composition of <i>Y. pseudotuberculosis</i> in<i></i> the development of antibiotic resistance, and (ii) the promising use of PBEH in combination therapy to increase the susceptibility of Gram-negative bacteria to the conventional β-lactam antibiotics, probably attenuating in vivo a previously demonstrated effect of LPE on the conformation and function of the OmpF channel.

scholarly journals Proteus mirabilis Targets Atherosclerosis Plaques in Human Coronary Arteries via DC-SIGN (CD209)

2021 ◽  
Vol 11 ◽  
Author(s):  
Ying Xue ◽  
Qiao Li ◽  
Chae Gyu Park ◽  
John D. Klena ◽  
Andrey P. Anisimov ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Coronary Arteries ◽  
Proteus Mirabilis ◽  
Chronic Infection ◽  
Intercellular Adhesion Molecule ◽  
Atherosclerotic Plaques ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Infection And Inflammation ◽  
Cluster Of Differentiation

Bacterial DNAs are constantly detected in atherosclerotic plaques (APs), suggesting that a combination of chronic infection and inflammation may have roles in AP formation. A series of studies suggested that certain Gram-negative bacteria were able to interact with dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin [DC-SIGN; cluster of differentiation (CD) 209] or langerin (CD207), thereby resulting in deposition of CD209s at infection sites. We wondered if Proteus mirabilis (a member of Proteobacteria family) could interact with APs through CD209/CD207. In this study, we first demonstrated that CD209/CD207 were also receptors for P. mirabilis that mediated adherence and phagocytosis by macrophages. P. mirabilis interacted with fresh and CD209s/CD207-expressing APs cut from human coronary arteries, rather than in healthy and smooth arteries. These interactions were inhibited by addition of a ligand-mimic oligosaccharide and the coverage of the ligand, as well as by anti-CD209 antibody. Finally, the hearts from an atherosclerotic mouse model contained higher numbers of P. mirabilis than that of control mice during infection-challenging. We therefore concluded that the P. mirabilis interacts with APs in human coronary arteries via CD209s/CD207. It may be possible to slow down the progress of atherosclerosis by blocking the interactions between CD209s/CD207 and certain atherosclerosis-involved bacteria with ligand-mimic oligosaccharides.

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