scholarly journals Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

2021 ◽  
Author(s):  
Jiangqin Song ◽  
Xiaolong Li ◽  
Xiaorong Hu ◽  
Yan Ding ◽  
Junyang Zhou ◽  
...  
Keyword(s):  
Causative Agent ◽  
Q Fever ◽  
Brucella Melitensis ◽  
Total Duration ◽  
Immunofluorescence Assay ◽  
Excessive Sweating ◽  
Case Presentation ◽  
Gram Negative ◽  
Missed Diagnosis ◽  
Loss Of Appetite

Abstract Background Rickettsia burneti is the causative agent of Q fever, Brucella melitensis is the causative agent of brucellosis, both of which are intracellular parasitic gram-negative bacteria. Rickettsia burneti and Brucella melitensis coinfection is fairly rarely reported in clinical. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Case Presentation Here, we report a case of Rickettsia burneti and Brucella melitensis co-infection. The patient is a 49-year-old sheepherder, was hospitalized for left forearm trauma. Three days after admission, the patient’s fever up to 39.0°C with excessive sweating, weakness, loss of appetite and headache. Rickettsia burneti IgM was detected positive by indirect immunofluorescence assay (IFA). After 72 hours blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. The patient accept therapy of doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for a total duration of four weeks. After receiving treatment, the patient’s symptoms disappeared rapidly, there has been no relapse or signs of chronic infection.Conclusion For high-risk practitioners, Q fever and brucellosis may be present in one patient, we should routinely test for both pathogens through a variety of tests to prevent missed diagnosis.

scholarly journals Imported brucellosis and Q-fever coinfection in Croatia: a case report

2018 ◽  
Vol 12 (06) ◽  
pp. 499-503
Author(s):  
Ljiljana Peric ◽  
Dario Sabadi ◽  
Ilija Rubil ◽  
Maja Bogdan ◽  
Marija Guzvinec ◽  
...  
Keyword(s):  
Case Report ◽  
Q Fever ◽  
Brucella Melitensis ◽  
16S Rrna Gene Sequencing ◽  
Immunofluorescence Assay ◽  
Rrna Gene ◽  
Excessive Sweating ◽  
First Case ◽  
Acute Q Fever ◽  
Loss Of Appetite

The brucellosis and Q-fever coinfection is very rarely reported. To our knowledge, this is the first case report of concomitant brucellosis and Q-fever, most likely imported in Croatia. A 30-year-old male agricultural worker was hospitalized on 22 April 2017 after a ten days fever up to 40°C with chills, shivering, excessive sweating, general weakness, loss of appetite and headache. A month and a half prior to the hospitalization he lost 18 kg of body weight. Three weeks before hospitalization the patient returned from Kupres (Bosnia and Herzegovina) where he was working for the past year on a sheep farm and consumed unpasteurized dairy products of sheep origin. At admission, his condition was moderately severe due to pronounced dehydration. Routine laboratory tests showed slightly elevated erythrocyte sedimentation rate, anemia, thrombocytopenia and elevated liver transaminases. The chest X-ray showed an inhomogeneous infiltrate of the lower right lung. Three sets of blood culture were cultivated. After 48 hours incubation, bacterial growth was detected in aerobic bottles. Gram-stained smear revealed small, gram-negative coccobacilli. Specimens were subcultured on blood and chocolate agar plates. Using a Vitek GN identification card, the isolated organism was identified as Brucella melitensis. 16S rRNA gene sequencing of the isolate confirmed it as a Brucella sp. Rose-Bengal test was positive, while Wright agglutination test showed a significant increase in antibody titer from 80 to 640 in paired sera. Using indirect immunofluorescence assay (IFA), Coxiella burnetii phase II IgM/IgG titers were 50 and 1024, respectively indicating acute Q-fever. The patient was treated with doxycycline and rifampicin. So far, there has been no relapse or signs of chronic infection.

scholarly journals Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jiangqin Song ◽  
Xiaorong Hu ◽  
Xiaolong Li ◽  
Youping Chen ◽  
Xiangyuan Yan ◽  
...  
Keyword(s):  
Chronic Infection ◽  
Culture Medium ◽  
Q Fever ◽  
Brucella Melitensis ◽  
Gram Negative Bacteria ◽  
Left Forearm ◽  
Poor Appetite ◽  
Gram Negative ◽  
Brucella Ovis ◽  
Missed Diagnosis

AbstractRickettsia is the pathogen of Q fever, Brucella ovis is the pathogen of brucellosis, and both of them are Gram-negative bacteria which are parasitic in cells. The mixed infection of rickettsia and Brucella ovis is rarely reported in clinic. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Here, we report a case of co-infection Rickettsia burneti and Brucella melitensis. The patient is a 49-year-old sheepherder, who was hospitalized with left forearm trauma. Three days after admission, the patient developed fever of 39.0°C, accompanied by sweating, fatigue, poor appetite and headache. Indirect immunofluorescence (IFA) was used to detect Rickettsia burneti IgM. After 72 hours of blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. Patients were treated with doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for 4 weeks. After treatment, the symptoms disappeared quickly, and there was no sign of recurrence or chronic infection. Q fever and Brucella may exist in high-risk practitioners, so we should routinely detect these two pathogens to prevent missed diagnosis.

scholarly journals Septic shock due to Yersinia pseudotuberculosis infection in an adult immunocompetent patient: a case report and literature review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Takehiro Hashimoto ◽  
Ryuichi Takenaka ◽  
Haruka Fukuda ◽  
Kazuhiko Hashinaga ◽  
Shin-ichi Nureki ◽  
...  
Keyword(s):  
Septic Shock ◽  
Therapeutic Strategy ◽  
Yersinia Pseudotuberculosis ◽  
Immunocompromised Host ◽  
Immunocompetent Patient ◽  
Case Presentation ◽  
Gram Negative ◽  
Old Japanese ◽  
The Right ◽  
Immunocompetent Patients

Abstract Background Yersinia pseudotuberculosis infection can occur in an immunocompromised host. Although rare, bacteremia due to Y. pseudotuberculosis may also occur in immunocompetent hosts. The prognosis and therapeutic strategy, especially for immunocompetent patients with Y. pseudotuberculosis bacteremia, however, remains unknown. Case presentation A 38-year-old Japanese man with a mood disorder presented to our hospital with fever and diarrhea. Chest computed tomography revealed consolidation in the right upper lobe with air bronchograms. He was diagnosed with pneumonia, and treatment with intravenous ceftriaxone and azithromycin was initiated. The ceftriaxone was replaced with doripenem and the azithromycin was discontinued following the detection of Gram-negative rod bacteria in 2 sets of blood culture tests. The isolated Gram-negative rod bacteria were confirmed to be Y. pseudotuberculosis. Thereafter, he developed septic shock. Doripenem was switched to cefmetazole, which was continued for 14 days. He recovered without relapse. Conclusions We herein report a case of septic shock due to Y. pseudotuberculosis infection in an adult immunocompetent patient. The appropriate microorganism tests and antibiotic therapy are necessary to treat patients with Y. pseudotuberculosis bacteremia.

scholarly journals De novoNAD synthesis is required for intracellular replication ofCoxiella burnetii, the causative agent of the neglected zoonotic disease Q fever

2018 ◽  
Vol 293 (48) ◽  
pp. 18636-18645 ◽  
Author(s):  
Mebratu A. Bitew ◽  
Chen Ai Khoo ◽  
Nitika Neha ◽  
David P. De Souza ◽  
Dedreia Tull ◽  
...  
Keyword(s):  
Causative Agent ◽  
Q Fever ◽  
Zoonotic Disease ◽  
Intracellular Replication

Cases of Q fever detected in residents of the novosibirsk region hospitalized with suspection of infections transmitted by ticks

2021 ◽  
Vol 66 (4) ◽  
pp. 229-236
Author(s):  
E. I. Bondarenko ◽  
E. S. Filimonova ◽  
E. I. Krasnova ◽  
E. V. Krinitsina ◽  
S. E. Tkachev
Keyword(s):  
Causative Agent ◽  
Q Fever ◽  
Clinical Manifestations ◽  
Test Methods ◽  
Pcr Analysis ◽  
Laboratory Diagnostics ◽  
Novosibirsk Region ◽  
Complex Approach ◽  
Wide Range ◽  
The Republic

Coxiella burnetii is the causative agent of Q fever (coxiellosis), which, in addition to acute manifestations, often occurs in a latent form, is prone to chronic course and, in the absence of antibiotic therapy, has a high risk of disability or death. As a result of the presence of a wide range of clinical manifestations specific to other infectious diseases, the use of laboratory test methods (LTM) is required to make a diagnosis. The presence of Q fever anthropurgic foci in the Novosibirsk region was described in the 90s of the last century, but due attention to its laboratory diagnostics is not paid in this region. The aim of the study was to identify genetic and serological markers of the causative agent, C. burnetii, in patients of the Novosibirsk region who were admitted for treatment with fever with suspected tick-borne infections (TBIs). DNA marker of the causative agent of Q fever was detected in blood samples by real time PCR in 9 out of 325 patients. In three patients, the presence of C. burnetii DNA was confirmed by sequencing of the IS1111 and htpB gene fragments. In ELISA tests, antibodies against the causative agent of coxiellosis were detected in the blood sera of 4 patients with positive results of PCR analysis. Contact with tick was registered in 7 out of 9 patients who had C. burnetii DNA and lacked markers of other TBIs. Six people were infected in the Novosibirsk region, two suffered from tick’s bite in Altai, and one case was from the Republic of Kyrgyzstan. Thus, a complex approach using both PCR analysis and ELISA provided the identification of markers of the Q fever causative agent in patients admitted with suspected TBIs, thereby differentiating it from other infections. Contact with ticks in most cases suggests that infection with C. burnetii had a transmissible pathway.

scholarly journals Genome Sequence of Pantoea ananatis LMG20103, the Causative Agent of Eucalyptus Blight and Dieback

2010 ◽  
Vol 192 (11) ◽  
pp. 2936-2937 ◽  
Author(s):  
Pieter De Maayer ◽  
Wai Yin Chan ◽  
Stephanus N. Venter ◽  
Ian K. Toth ◽  
Paul R. J. Birch ◽  
...  
Keyword(s):  
South Africa ◽  
Genome Sequence ◽  
Causative Agent ◽  
Plant Pathogen ◽  
Human Disease ◽  
Host Plants ◽  
Pantoea Ananatis ◽  
Gram Negative

ABSTRACT Pantoea ananatis is a Gram-negative plant pathogen that causes disease on a broad range of host plants, including pineapple, maize, rice, onion, melons, and Eucalyptus, and has been implicated in several cases of human disease. Here, we report the genome sequence of P. ananatis LMG20103 isolated from diseased Eucalyptus in South Africa.

scholarly journals Complete Genome Sequence ofBartonella ancashensisStrain 20.00, Isolated from the Blood of a Patient with Verruga Peruana

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Jun Hang ◽  
Kristin E. Mullins ◽  
Robert J. Clifford ◽  
Fatma Onmus-Leone ◽  
Yu Yang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Causative Agent ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Bartonella Bacilliformis ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus

Here we present the complete genome sequence ofBartonella ancashensisstrain 20.00, isolated from the blood of a Peruvian patient with verruga peruana, known as Carrion’s disease.Bartonella ancashensisis a Gram-negative bacillus, phylogenetically most similar toBartonella bacilliformis, the causative agent of Oroya fever and verruga peruana.

scholarly journals THE INFECTION WITH HELICOBACTER PYLORI FAVORS THE PRESENCE OF GIARDIA LAMBLIA IN THE GASTRIC MUCOSA – CASE PRESENTATION

2016 ◽  
Vol 19 (3) ◽  
pp. 78-82
Author(s):  
Meliț Lorena Elena ◽  
◽  
Cristina Oana Mărginean ◽  
Simona Mocan ◽  
Nicoleta Suciu ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Giardia Lamblia ◽  
Eradication Therapy ◽  
Gastrointestinal Symptoms ◽  
Case Presentation ◽  
Acid Environment ◽  
Antiparasitic Drugs ◽  
Loss Of Appetite ◽  
Favorable Conditions

Helicobacter pylori and Giardia lamblia are common worldwide. Helicobacter pylori is a favoring factor for the presence of Giardia lamblia in the stomach due to the neutralization of the gastric pH through the secretion of urease. We present the case of a 5-year-old child, with intermittent gastro-intestinal symptoms in the recent personal pathological history, admitted in the Pediatrics Clinic 1 Târgu Mureș for abdominal pain, nausea, loss of appetite and hematemesis. The superior digestive endoscopy revealed multiple hemorrhagic lesions of the gastric mucosa, and the pathological exam of the antral gastric mucosa identified the coexistence of Helicobacter pylori and Giardia lamblia. The evolution of the patient was favorable under the eradication therapy of the infection with Helicobacter pylori and the antiparasitic drugs, the control endoscopy revealing a gastric mucosa without macroscopically obvious modifications, and the pathological re-evaluation pointed out regenerative modifications of the gastric mucosa. The particularity of the case is represented by the identification of the parasite Giardia lamblia in the gastric mucosa, a physiologically acid environment, alkalinized by the urease secreted by Helicobacter pylori, therefore providing favorable conditions for the development of this parasite in a 5-year-old child, from a favorable socio-economic environment, with intermittent gastrointestinal symptoms in his recent personal pathological history.

scholarly journals Q Fever in Pregnancy—Case Presentation and Literature Review

2013 ◽  
Vol 04 (08) ◽  
pp. 364-368
Author(s):  
Kugler Eitan ◽  
Amital Howard ◽  
Alon Danny
Keyword(s):  
Literature Review ◽  
Q Fever ◽  
Case Presentation ◽  
In Pregnancy

scholarly journals A case of Brucellosis with Large Periaortic Mass And Secondary Vasculitis In A Patient Presenting Intermittent Abdominal Pain

2021 ◽  
Author(s):  
Huilan Liu ◽  
Yutong Zhang ◽  
Xiaomei Leng ◽  
Yunjiao Yang ◽  
Xiaofeng Zeng
Keyword(s):  
Abdominal Pain ◽  
Clinical Manifestations ◽  
Detailed Examination ◽  
Blood Cultures ◽  
Organ System ◽  
High Morbidity ◽  
Case Presentation ◽  
Intermittent Abdominal Pain ◽  
Cardiovascular Involvement ◽  
Missed Diagnosis

Abstract Background Brucellosis is still highly prevalent and causes high morbidity. It can involve any organ system and has been implicated in protean complications. Cardiovascular involvement is the main cause of mortality. This case described a female patient infected by Brucella with large periaortic mass and secondary vasculitis whose manifestation was abdominal pain. The aim was to raise the awareness of earlier recognition of Brucella infections without fever and remitting risk by taking appropriate treatments for those with cardiovascular involvement.Case presentation A case was reported regarding a female present intermittent abdominal pain. Two months later, she was admitted to the Department of Rheumatology and Immunology in our hospital due to high inflammatory markers, the large periaortic mass and vasculitis manifestations in aortic computed tomography angiography. By detailed examination, Brucellosis was highly suggestive based on positive blood cultures. Finally, no discomfort has ever occurred, and the mass was prominently reduced after antibiotic therapy of half a year.Conclusions The clinical manifestations of brucellosis are protean. For those without fever and cardiovascular involvement as the main manifestation, especially vasculitis, we must not just set the standard rigidly, instead, it is recommended to carry out the relevant differential diagnosis to avoid misdiagnosis or missed diagnosis.

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