scholarly journals 84 A universal screening culture medium for polymyxin-resistant Gram-negative bacteria

2016 ◽  
Vol 15 ◽  
pp. S72 ◽  
Author(s):  
P. Nordmann ◽  
A. Jayol ◽  
L. Poirel
Keyword(s):  
Universal Screening ◽  
Culture Medium ◽  
Gram Negative Bacteria ◽  
Gram Negative

scholarly journals Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jiangqin Song ◽  
Xiaorong Hu ◽  
Xiaolong Li ◽  
Youping Chen ◽  
Xiangyuan Yan ◽  
...  
Keyword(s):  
Chronic Infection ◽  
Culture Medium ◽  
Q Fever ◽  
Brucella Melitensis ◽  
Gram Negative Bacteria ◽  
Left Forearm ◽  
Poor Appetite ◽  
Gram Negative ◽  
Brucella Ovis ◽  
Missed Diagnosis

AbstractRickettsia is the pathogen of Q fever, Brucella ovis is the pathogen of brucellosis, and both of them are Gram-negative bacteria which are parasitic in cells. The mixed infection of rickettsia and Brucella ovis is rarely reported in clinic. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Here, we report a case of co-infection Rickettsia burneti and Brucella melitensis. The patient is a 49-year-old sheepherder, who was hospitalized with left forearm trauma. Three days after admission, the patient developed fever of 39.0°C, accompanied by sweating, fatigue, poor appetite and headache. Indirect immunofluorescence (IFA) was used to detect Rickettsia burneti IgM. After 72 hours of blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. Patients were treated with doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for 4 weeks. After treatment, the symptoms disappeared quickly, and there was no sign of recurrence or chronic infection. Q fever and Brucella may exist in high-risk practitioners, so we should routinely detect these two pathogens to prevent missed diagnosis.

scholarly journals Antiadherent and Antibiofilm Activity ofHumulus lupulusL. Derived Products: New Pharmacological Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Marcin Rozalski ◽  
Bartlomiej Micota ◽  
Beata Sadowska ◽  
Anna Stochmal ◽  
Dariusz Jedrejek ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Culture Medium ◽  
Antimicrobial Properties ◽  
Antibiofilm Activity ◽  
Pharmacological Properties ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Abiotic Surfaces ◽  
First Time ◽  
Plant Compounds

New antimicrobial properties of products derived fromHumulus lupulusL. such as antiadherent and antibiofilm activities were evaluated. The growth of gram-positive but not gram-negative bacteria was inhibited to different extents by these compounds. An extract of hop cones containing 51% xanthohumol was slightly less active againstS. aureusstrains (MIC range 31.2–125.0 μg/mL) than pure xanthohumol (MIC range 15.6–62.5 μg/mL). The spent hop extract, free of xanthohumol, exhibited lower but still relevant activity (MIC range 1-2 mg/mL). There were positive coactions of hop cone, spent hop extracts, and xanthohumol with oxacillin against MSSA and with linezolid against MSSA and MRSA. Plant compounds in the culture medium at sub-MIC concentrations decreased the adhesion ofStaphylococcito abiotic surfaces, which in turn caused inhibition of biofilm formation. The rate of mature biofilm eradication by these products was significant. The spent hop extract at MIC reduced biofilm viability by 42.8%, the hop cone extract by 74.8%, and pure xanthohumol by 86.5%. When the hop cone extract or xanthohumol concentration was increased, almost complete biofilm eradication was achieved (97–99%). This study reveals the potent antibiofilm activity of hop-derived compounds for the first time.

scholarly journals A Selective Culture Medium for Screening Ceftazidime-Avibactam Resistance in Enterobacterales and Pseudomonas aeruginosa

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Mustafa Sadek ◽  
Laurent Poirel ◽  
Camille Tinguely ◽  
Patrice Nordmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lower Limit ◽  
Culture Medium ◽  
Limit Of Detection ◽  
Resistance Mechanisms ◽  
Gram Negative Bacteria ◽  
Excellent Performance ◽  
Gram Negative ◽  
Content Type ◽  
Selective Culture Medium

ABSTRACT The SuperCAZ/AVI medium was developed for screening ceftazidime-avibactam (CZA) resistance among Gram-negative bacteria (Enterobacterales and Pseudomonas aeruginosa). It was evaluated using 50 CZA-susceptible and 42 CZA-resistant Gram-negative isolates. Its sensitivity and specificity of detection were 100%. Excellent performance of the medium was also observed by testing spiked stools, with the lower limit of detection ranging from 101 to 102 CFU/ml. This screening medium provides the opportunity to detect CZA-resistant isolates regardless of their resistance mechanisms.

Relationship between Adaptive Changing of Lysophosphatidylethanolamine Content in the Bacterial Envelope and Ampicillin Sensitivity of Yersinia pseudotuberculosis

2018 ◽  
Vol 28 (5) ◽  
pp. 236-239
Author(s):  
Nina Sanina ◽  
Lyudmila Pomazenkova ◽  
Svetlana Bakholdina ◽  
Natalia Chopenko ◽  
Anna Zabolotnaya ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Culture Medium ◽  
Bacterial Resistance ◽  
Yersinia Pseudotuberculosis ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Control Value ◽  
And Function

The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in <i>Yersinia pseudotuberculosis</i> induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content affect the resistance of bacteria to ampicillin. The addition of glucose to the culture medium was shown to simultaneously increase the level of LPE and minimum inhibitory concentration (MIC) for ampicillin of <i>Y. pseudotuberculosis</i> cells 6- and 2-fold, respectively. However, the coadministration of glucose and polyphenol extract from buckwheat husks reduced the content of LPE 2-fold and restored MIC to the control value. Thus, PBEH can be used as antibiotic adjuvant to improve an antibiotic’s ability to cross the outer membrane. The present work demonstrated: (i) the role of adaptive changes in the lipid composition of <i>Y. pseudotuberculosis</i> in<i></i> the development of antibiotic resistance, and (ii) the promising use of PBEH in combination therapy to increase the susceptibility of Gram-negative bacteria to the conventional β-lactam antibiotics, probably attenuating in vivo a previously demonstrated effect of LPE on the conformation and function of the OmpF channel.

scholarly journals A Universal Culture Medium for Screening Polymyxin-Resistant Gram-Negative Isolates

2016 ◽  
Vol 54 (5) ◽  
pp. 1395-1399 ◽  
Author(s):  
Patrice Nordmann ◽  
Aurélie Jayol ◽  
Laurent Poirel
Keyword(s):  
Sensitivity And Specificity ◽  
Culture Medium ◽  
Resistant Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Universal Culture

The colistin-containing SuperPolymyxin medium was developed for screening polymyxin-resistant Gram-negative bacteria. It was evaluated with 88 polymyxin-susceptible or polymyxin-resistant cultured Gram-negative isolates. Its sensitivity and specificity of detection were ca. 100%. The SuperPolymyxin medium is the first screening medium that is able to detect intrinsic and acquired polymyxin-resistant bacteria.

scholarly journals A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria

2018 ◽  
Vol 91 (2) ◽  
pp. 118-122 ◽  
Author(s):  
Patrice Nordmann ◽  
Alain Mazé ◽  
Esther Culebras ◽  
Jan Dobias ◽  
Aurélie Jayol ◽  
...  
Keyword(s):  
16S Rrna ◽  
Culture Medium ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
16S Rrna Methylase

Bacterial-Mucosal Cell Junctional Complexes

1974 ◽  
Vol 32 ◽  
pp. 144-145
Author(s):  
Roger C. Wagner
Keyword(s):  
Intestinal Wall ◽  
Rat Colon ◽  
Mucosal Cell ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Mucosal Cells ◽  
Mucosal Lining ◽  
Degenerative Alterations ◽  
Junctional Complexes ◽  
Intestinal Mucosal Cells

Bacteria exhibit the ability to adhere to the apical surfaces of intestinal mucosal cells. These attachments either precede invasion of the intestinal wall by the bacteria with accompanying inflammation and degeneration of the mucosa or represent permanent anchoring sites where the bacteria never totally penetrate the mucosal cells.Endemic gram negative bacteria were found attached to the surface of mucosal cells lining the walls of crypts in the rat colon. The bacteria did not intrude deeper than 0.5 urn into the mucosal cells and no degenerative alterations were detectable in the mucosal lining.

Rapid demonstration of septic or infectious arthritis due to Gram-negative bacteria

1992 ◽  
Vol 50 (1) ◽  
pp. 686-687
Author(s):  
Jacob S. Hanker ◽  
Paul R. Gross ◽  
Beverly L. Giammara
Keyword(s):  
Chronic Arthritis ◽  
Blood Cultures ◽  
Gram Negative Bacteria ◽  
Infectious Arthritis ◽  
Bacterial Arthritis ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Blood cultures are positive in approximately only 50 per cent of the patients with nongonococcal bacterial infectious arthritis and about 20 per cent of those with gonococcal arthritis. But the concept that gram-negative bacteria could be involved even in chronic arthritis is well-supported. Gram stains are more definitive in staphylococcal arthritis caused by gram-positive bacteria than in bacterial arthritis due to gram-negative bacteria. In the latter situation where gram-negative bacilli are the problem, Gram stains are helpful for 50% of the patients; they are only helpful for 25% of the patients, however, where gram-negative gonococci are the problem. In arthritis due to gram-positive Staphylococci. Gramstained smears are positive for 75% of the patients.

Ultrastructure of periplasmic bodies in gram-negative bacteria

1990 ◽  
Vol 48 (3) ◽  
pp. 640-641
Author(s):  
Xie Nianming ◽  
Ding Shaoqing ◽  
Wang Luping ◽  
Yuan Zenglin ◽  
Zhan Guolai ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Campylobacter Jejuni ◽  
Proteus Mirabilis ◽  
Shigella Flexneri ◽  
Morphological Description ◽  
Gram Negative Bacteria ◽  
Periplasmic Space ◽  
Clostridium Tetani ◽  
Gram Negative ◽  
Brucella Canis

Perhaps the data about periplasmic enzymes are obtained through biochemical methods but lack of morphological description. We have proved the existence of periplasmic bodies by electron microscope and described their ultrastructures. We hope this report may draw the attention of biochemists and mrophologists to collaborate on researches in periplasmic enzymes or periplasmic bodies with each other.One or more independent bodies may be seen in the periplasmic space between outer and inner membranes of Gram-negative bacteria, which we called periplasmic bodies. The periplasmic bodies have been found in seven species of bacteria at least, including the Pseudomonas aeroginosa. Shigella flexneri, Echerichia coli. Yersinia pestis, Campylobacter jejuni, Proteus mirabilis, Clostridium tetani. Vibrio cholerae and Brucella canis.

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