scholarly journals Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
L. Fattorini ◽  
A. Veloccia ◽  
F. Della Rovere ◽  
S. D’Angeli ◽  
G. Falasca ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Rooting ◽  
Anthranilate Synthase ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Indole 3 Acetic Acid ◽  
Stimulation Of

Identification of IAA-regulated genes in Pseudomonas syringae pv. tomato strain DC3000

2021 ◽  
Author(s):  
Arnaud-Thierry Djami-Tchatchou ◽  
Zipeng Alex Li ◽  
Paul Stodghill ◽  
Melanie J. Filiatrault ◽  
Barbara N. Kunkel
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Stress Response ◽  
Pseudomonas Syringae ◽  
Plant Pathogen ◽  
Type Iii Secretion ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid ◽  
Exogenous Iaa

The auxin indole-3-acetic acid (IAA) is a plant hormone that not only regulates plant growth and development but also plays important roles in plant-microbe interactions. We previously reported that IAA alters expression of several virulence-related genes in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000 ( Pto DC3000). To learn more about the impact of IAA on regulation of Pto DC3000 gene expression we performed a global transcriptomic analysis of bacteria grown in culture, in the presence or absence of exogenous IAA. We observed that IAA repressed expression of genes involved in the Type III secretion (T3S) system and motility and promoted expression of several known and putative transcriptional regulators. Several of these regulators are orthologs of factors known to regulate stress responses and accordingly expression of several stress response-related genes was also upregulated by IAA. Similar trends in expression for several genes were also observed by RT-qPCR. Using an Arabidopsis thaliana auxin receptor mutant that accumulates elevated auxin, we found that many of the P. syringae genes regulated by IAA in vitro were also regulated by auxin in planta . Collectively the data indicate that IAA modulates many aspects of Pto DC3000 biology, presumably to promote both virulence and survival under stressful conditions, including those encountered in or on plant leaves. IMPORTANCE Indole-3-acetic acid (IAA), a form of the plant hormone auxin, is used by many plant-associated bacteria as a cue to sense the plant environment. Previously, we showed that IAA can promote disease in interactions between the plant pathogen Pseudomonas syringae strain Pto DC000 and one of its hosts, Arabidopsis thaliana . However, the mechanisms by which IAA impacts the biology of Pto DC3000 and promotes disease are not well understood. Here we demonstrate that IAA is a signal molecule that regulates gene expression in Pto DC3000. The presence of exogenous IAA affects expression of over 700 genes in the bacteria, including genes involved in Type III secretion and genes involved in stress response. This work offers insight into the roles of auxin promoting pathogenesis.

scholarly journals Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN

2013 ◽  
Vol 26 (5) ◽  
pp. 546-553 ◽  
Author(s):  
Ana Zúñiga ◽  
María Josefina Poupin ◽  
Raúl Donoso ◽  
Thomas Ledger ◽  
Nicolás Guiliani ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Quorum Sensing ◽  
Plant Growth ◽  
Growth Promotion ◽  
Auxin Signaling ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
Burkholderia Phytofirmans Psjn

Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant–bacteria association. Burkholderia phytofirmans PsJN is a well-known plant-growth-promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essential for plant-growth-promoting effects produced by bacteria. Using appropriate mutant strains of B. phytofirmans, we obtained evidence for the importance of N-acyl homoserine lactone-mediated (quorum sensing) cell-to-cell communication in efficient colonization of Arabidopsis thaliana plants and the establishment of a beneficial interaction. We also observed that bacterial degradation of the auxin indole-3-acetic acid (IAA) plays a key role in plant-growth-promoting traits and is necessary for efficient rhizosphere colonization. Wildtype B. phytofirmans but not the iacC mutant in IAA mineralization is able to restore promotion effects in roots of A. thaliana in the presence of exogenously added IAA, indicating the importance of this trait for promoting primary root length. Using a transgenic A. thaliana line with suppressed auxin signaling (miR393) and analyzing the expression of auxin receptors in wild-type inoculated plants, we provide evidence that auxin signaling in plants is necessary for the growth promotion effects produced by B. phytofirmans. The interplay between ethylene and auxin signaling was also confirmed by the response of the plant to a 1-aminocyclopropane-1-carboxylate deaminase bacterial mutant strain.

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