scholarly journals Effects of sevoflurane exposure on apoptosis and cell cycle of peripheral blood lymphocytes, and immunologic function

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhimin Ji ◽  
Wanjun Wu ◽  
Fan Zhou ◽  
Junfang Hu ◽  
Qiuping Xu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Operating Room ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Venous Blood ◽  
Peripheral Blood Lymphocytes ◽  
Exposed Group ◽  
Control Group ◽  
Blood Lymphocytes

Abstract Background Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room. Methods A cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident’s breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry. Results The average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05). Conclusions Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples. Trial registration number and date of registration ChiCTR2000040772, December 9, 2020 (Retrospective registration).

scholarly journals Effects of Sevoflurane Exposure on Apoptosis and Cell Cycle of Peripheral Blood Lymphocytes, and Immunologic Function

2021 ◽  
Author(s):  
zhimin ji ◽  
Wanjun Wu ◽  
Fan zhou ◽  
Junfang Hu ◽  
Qiuping Xu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Occupational Exposure ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Peripheral Blood Lymphocytes ◽  
Exposed Group ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Significant Difference

Abstract Background: Waste anesthetic gases (WAGS) leaked from new-type halogenated inhalational anesthetics such as sevoflurane were reported to pose a risk for the health of operation room personnel. The effects of WAGS on peripheral blood lymphocytes remain yet controversial. Purpose: The present study was undertaken to examine whether occupational exposure to sevoflurane has detrimental effects on the peripheral blood lymphocytes of exposed medical personnel in vivo. Methods: A cohort of 56 medical residents were divided into exposed group (n=28) and control group (non-exposed group) (n=28). Gas chromatograph was used to measure the concentration of sevoflurane in the medical resident’s breathing zone during surgeries under inhalation anesthesia in exposure group. The collection time lasted for one hour. Peripheral blood lymphocytes were isolated from venous blood and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry.Results: The average concentration of sevoflurane in exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. Sevoflurane had no significant effect on the apoptosis and cell cycle of peripheral blood lymphocytes in the exposed group relative to the control group (P>0.05). Similarly, there was no significant difference in the lymphocyte subsets and the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P>0.05).Conclusion: Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples.Trial registration number and date of registration：ChiCTR2000040772, December 9, 2020 (Retrospective registration)

scholarly journals CHANGES OF CYCLIN D1-DEPENDENT REGULATION OF CELL CYCLE IN PERIPHERAL BLOOD LYMPHOCYTES OF CHORNOBYL CLEAN-UP WORKERS AT A REMOTE PERIOD AFTER RADIATION EXPOSURE

2020 ◽  
Vol 25 ◽  
pp. 430-442
Author(s):  
N. Golyarnik ◽  
◽  
I. Ilyenko ◽  
L. Zvarych ◽  
D. Bazyka ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Radiation Exposure ◽  
Cyclin D1 ◽  
Peripheral Blood ◽  
Genome Instability ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Proliferative Potential ◽  
Blood Lymphocytes ◽  
Regulation Of Cell Cycle

Objective. To study proliferative potential of peripheral blood lymphocytes of Chornobyl clean-up workers by level of expression of cyclin D1 and quantitative parameters of cell cycle at a remote period after radiation exposure. Materials and methods. The research subject was the peripheral blood lymphocytes (PB) of Chornobyl clean-up workers 30–33 years after radiation exposure. A total of 207 men were surveyed, 164 of them were clean-up workers exposed in the dose range 10.43–3623.31 mSv and 43 persons of the control group. Analysis of proliferation potential (cell cycle initiation) and cyclin D1 expression in PB lymphocytes were performed in vitro by a micro method of whole blood leukocytes culture with phytohemagglutinine-P (PHA). Sample preparation was performed by a standard immunofluorescent assay for intracellular proteins using the FITC labelled Mouse Anti-Human Cyclin D1 Antibody Set. Cell distribution by cell cycle phases studied by propidium iodide DNA staining and analysis on FACSCalibur laser flow cytometer in histogram mode with separation of G0/G1-, S- and G2/M-regions and Sub-G0/G1- region (apoptotic cells). Results and conclusions. An increase in the level of spontaneous сyclin D1 expression and disturbance of сyclin D1-dependent regulation of cell cycle of PB lymphocytes after mitogen activation were determined in a remote period after radiation exposure. An increase in the level of cyclin D1 expression was accompanied by increase in pool of cells in the S- and G2/M-phases of cell cycle which characterizes the high proliferative potential of PB lymphocytes. Mitogen-induced delay of cell cycle of lymphocytes in G1/S check point and reduction of S-phase was revealed. These changes are a manifestation of genomic instability caused by the effect of radiation and depend on the radiation dose. The results confirm the hypothesis about the significance of levels of cyclin D1 expression, as a criterion for manifestations of genome instability and risks of oncogenesis in a remote period after irradiation. Key words: cell cycle, cell proliferation, cyclin D1, genome instability, radiation exposure, Chornobyl clean-up workers.

scholarly journals Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising Radiation

2008 ◽  
Vol 59 (4) ◽  
pp. 251-259 ◽  
Author(s):  
Vilena Kašuba ◽  
Ružica Rozgaj ◽  
Anamarija Jazbec
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Direct Detection ◽  
Hospital Staff ◽  
Peripheral Blood Lymphocytes ◽  
Ionising Radiation ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Occupationally Exposed ◽  
Low Levels

Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising RadiationMedical staff is an occupational group exposed to different agents suspected to induce genetic damage. Among them ionising radiation is the most studied. Cytogenetic analysis of human chromosomes in peripheral lymphocytes allows direct detection of mutation in somatic cells. This study investigated the cytogenetic effects of low-level ionising x-radiation in 48-hour peripheral blood lymphocyte cultures sampled from 765 hospital staff occupationally exposed to several agents known or suspected to induce chromosome damage and compared them with 200 control subjects. The exposed subjects were divided in eight (8) groups according to their specialities and job titles. The exposed groups manifested an increase in all types of chromosome aberrations. Acentric fragments were the most frequent chromosome-type aberration. Dicentric chromosomes were statistically significant only in urologists/gynaecologists. Age and smoking significantly influenced the incidence of dicentrics in the exposed groups. The frequency of ring chromosomes was low in all exposed groups (range: 0-2), and none were found in the control group. These findings indicate the importance of periodic medical checkups of hospital staff occupationally exposed to low doses of ionising radiation. The purpose is to create an individual cytogenetic register, where changes could evidence individual risks.

Aluminum-induced micronuclei and apoptosis in human peripheral-blood lymphocytes treated during different phases of the cell cycle

2005 ◽  
Vol 20 (4) ◽  
pp. 402-406 ◽  
Author(s):  
Anna Banasik ◽  
Anna Lankoff ◽  
Agnieszka Piskulak ◽  
Katarzyna Adamowska ◽  
Halina Lisowska ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes
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