scholarly journals Impact of dorzolamide, benzalkonium-preserved dorzolamide and benzalkonium-preserved brinzolamide on selected biomarkers of oxidative stress in the tear film

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lech Sedlak ◽  
Marta Świerczyńska ◽  
Weronika Borymska ◽  
Maria Zych ◽  
Dorota Wyględowska-Promieńska
Keyword(s):  
Oxidative Stress ◽  
Benzalkonium Chloride ◽  
Tear Film ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Advanced Oxidation Protein Products ◽  
Oxidative Stress Biomarkers ◽  
Oxidative Stress Index ◽  
Stress Biomarkers ◽  
Oxidant Status

Abstract Background Long-term use of topical, especially benzalkonium chloride (BAC)-preserved, antiglaucoma medications can cause a negative impact on the ocular surface. The aim of the study was to assess the effect of topical carbonic anhydrase inhibitors (CAIs) on selected oxidative stress biomarkers in the tear film. Methods The patients were divided into four sex-matched groups: group C (n = 25) – control group – subjects who did not use topical antiglaucoma medications, group DL (n = 14) – patients using preservative-free dorzolamide, group DL + BAC (n = 16) – patients using topical BAC-preserved dorzolamide, group BL + BAC (n = 17) – patients using BAC-preserved brinzolamide. Subjects in all the study groups have been using the eye drops two times daily for 6–12 months. The oxidative stress biomarkers in the tear film samples were measured: total protein (TP) concentration, advanced oxidation protein products (AOPP) content, total sulfhydryl (-SH) groups content, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as Total Oxidant Status (TOS), Total Antioxidant Response (TAR), and Oxidative Stress Index (OSI). Results The advanced oxidation protein products content, Total Oxidant Status as well as superoxide dismutase and catalase activities in the group DL + BAC and BL + BAC were higher in comparison with the group C. The total sulfhydryl groups content was lower in the group DL + BAC and BL + BAC when compared to group C. Oxidative Stress Index was higher in the groups DL + BAC and BL + BAC in comparison with the groups DL and C. Conclusions Use of topical benzalkonium chloride-preserved carbonic anhydrase inhibitors increases oxidative stress in the tear film.

scholarly journals Antioxidant Potentials of Aqueous and Ethanolic Extracts of Morus mesozygia Linn Stapf Twigs in Streptozotocin-Induced Diabetic Rats

2021 ◽  
pp. 36-46
Author(s):  
Marcella Tari Joshua ◽  
Edna O. Wachuku ◽  
N. Boisa ◽  
Nsirim Nduka
Keyword(s):  
Oxidative Stress ◽  
Diabetic Rats ◽  
Stress Index ◽  
Albino Rats ◽  
Total Oxidant Status ◽  
Oxidative Stress Index ◽  
Methanolic Extracts ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
Antioxidant Effects

Aim: The aim of this study was to assess the antioxidant effects of aqueous, ethanolic and methanolic extracts of Morus mesozygia Linn. Stapf. Twigs in Streptozotocin-Induced Diabetic Rats. Study Design: The study is an experimental case-controlled study. Place and Duration of Study: This study was carried out at the Biochemistry Research Laboratory, University of Port Harcourt, Rivers State, Nigeria, between June, 2018 and April, 2019. Methodology: A total of 65 male albino rats that weighed between 150g to 200g were used for this research study. Three different extracted solvents; aqueous, ethanolic and methanolic twig extracts were administered to different groups of the rats. The male albino rats for this study were induced with a single dose of 40mg/kg b.wt, intraperitoneally of streptozotocin in 0.1M of citrate buffer, pH 4.5. The diabetic male rats were those whose fasting blood glucose (FBG) were from 250mg/dl or 13mmol/L and above. They were then divided into different groups and treated with different concentrations of aqueous, ethanolic and methanolic extracts of the plant material. At the end of treatment period, the rats were kept on fasting for 6 hours prior to the process of euthanasia, they were sacrificed and blood samples were collected through cardiac puncture for analysis into lithium heparin bottle for the estimation of oxidative stress markers, malondaldehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), and antioxidant enzymes, superoxide dismutase (SOD), total antioxidant capacity (TAS). Statistical analysis was done using GraphPad prism (version 6.1) software. Data generated were represented as mean and standard deviations (Mean ±S. D). Level of significant at Tukey’s Multiple Comparative Test was tested at p<0.0001. Charts were made possible with the application of Minitab version 2019. Results: The results showed that there were significant increases in the levels of superoxide dismutase (SOD, 414.2±1.30) ng/ml, total antioxidant status (TAS, 82.97±7.71) mU/ml, total oxidant status (TOS, 355.02± 14.02) mU/ml activity, a reduced oxidative stress index of 4.29±0.26 and concentration of malondialdehyde (MDA of 18.67± 0.26mmol/L) when rats were treated with 400mg/kg of aqueous leaves of Morus mesozygia Linn. S. When compared with those of rats treated with 200mg/kg of aqueous leaf extracts of MMLS. there was a significant increases and decreases respectively. Other methods of extractions (methanolic and ethanolic), also improved the antioxidant statuses of the diabetes induced and treated rats after treatment of the extracts. Conclusion: The three extracts of Morus mesozygia Linn. S showed tremendous antioxidant effects against Streptozotocin-induced diabetic rats, with the methanolic extract showing the most potent effect.

scholarly journals Biochemical Evaluation of the Effects of Quercetin On Experimental Acute Methanol Intoxication in Rats

2020 ◽  
pp. 195-201
Author(s):  
Hasan Hüseyin Kozak ◽  
İbrahim Kılınç ◽  
Alpaslan Özkürkçüler
Keyword(s):  
Oxidative Stress ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Maximum Serum ◽  
Methanol Intoxication ◽  
Oxidative Stress Index ◽  
Group A ◽  
Anti Oxidant ◽  
Level Elevation ◽  
Oxidant Status

Previous studies have shown the role of oxidative stress in methanol toxicity. Also, studies have shown antioxidant, anti-inflammatory effects of quercetin (Q). This study evaluates the effect of quercetin (Q) administration on total oxidant status (TOS), total anti-oxidant status (TAS), oxidative stress index (OSI) and paraoxanase 1 (PON1) levels in rats with experimentally-induced methanol (MeOH) intoxication. Six groups were constituted as control, methotrexate (Mtx), Mtx+MeOH, Mtx+MeOH+ethanol (EtOH), Mtx+MeOH+Q1, Mtx+MeOH+Q2. All rats except controls were injected Mtx (0.3 mg/kg daily) intra-peritoneally (IP) for 7 days. On the 8th day of the test, 3 g/kg MeOH was injected IP in MeOH, EtOH and Q groups. Four hours after MeOH administration, 0.5 g/kg EtOH was injected IP in EtOH group and 50 mg/kg Q was administered IP in Q1 and Q2 groups. In addition, a total of 5 doses of 50 mg/kg Q was injected IP 24, 48, 72 and 96 hours after the first dose in Q2 group. Saline solution was given IP in the other groups. Rats were sacrificed with anesthesia 8 hours after the administrations. Blood samples were obtained for evaluating total oxidant status (TOS), total anti-oxidant status (TAS), oxidative stress index (OSI) and paraoxanase 1 (PON1) levels. The highest TOS level was found in MeOH+MTx group. A significant reduction was detected in serum TOS levels in MeOH+Mtx+EtOH, MeOH+Mtx+Q1 and MeOH+Mtx+Q2 groups. The lowest serum TAS level was detected in MeOH+Mtx group. Maximum TAS level elevation was found in MeOH+Mtx+Q2, MeOH+Mtx+Q1 and MeOH+Mtx+EtOH groups. The highest OSI ratio was found in MeOH+Mtx group. A reduction was detected in OSI ratios in MeOH+Mtx+EtOH, MeOH+Mtx+Q1 and MeOH+Mtx+Q2 groups as compared to MeOH+Mtx group. The lowest serum PON1 level was found in MeOH+Mtx group. Maximum serum PON1 level elevation was found in MeOH+Mtx+Q2 group. The results indicating that quercetin administration could be effective on both acute and subacute processes of methanol intoxication were tried to be revealed through serum TOS, TAS, OSI and PON1 levels. These results show that quercetin could be used as an alternative treatment option in methanol intoxication.

scholarly journals Antioxidant and Oxidant Status of Endemic Helleborus vesicarius

2020 ◽  
Vol 8 (9) ◽  
pp. 2008-2010
Author(s):  
Falah Saleh Mohammed ◽  
Gülcan Çınar ◽  
Serap Sahin Yigit ◽  
Hasan Akgül ◽  
Muhittin Dogan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Oxidative Stress Index ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

Plants are important antioxidant sources. In our study, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of endemic Helleborus vesicarius Aucher ex Boiss.were determined. The aerial parts of the plant samples were dried and extracted with ethanol (EtOH). TAS and TOS values of plant extract were determined using Rel Assay Diagnostics kits. As a result of the studies, the TAS value of H. vesicarius was determined 5.548±0.23, the TOS value was 13.778±0.119 and the OSI value was 0.249±0.009. In this context, the plant has been shown to have significant antioxidant potential.

scholarly journals Evaluation of oxidative stress in sheep infected with Psoroptes ovis using total antioxidant capacity, total oxidant status, and malondialdehyde level

2017 ◽  
Vol 61 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Mustafa Sinan Aktas ◽  
Fatih Mehmet Kandemir ◽  
Akin Kirbas ◽  
Basak Hanedan ◽  
Mehmet Akif Aydin
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Psoroptes Ovis ◽  
Oxidative Stress Index ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

AbstractIntroduction:The study aimed at evaluating oxidative stress using malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) markers in sheep naturally infected withPsoroptes ovis(Acari).Material and Methods:The study was performed on 40 sheep divided into two equal groups: a healthy group (group I) and a group naturally infected withPsoroptes ovis(group II). The sera were obtained by centrifuging blood samples collected from the vena jugularis and serum MDA level changes in the samples were measured spectrophotometrically. Commercially available test kits were used for the measurement of TAC and TOS levels. The percentage ratio of TOS level to TAC level was accepted as OSI.Results:The serum malondialdehyde, total oxidant status levels, and oxidative stress index increased significantly (P < 0.01) in group II, while the serum total antioxidant capacity levels decreased significantly (P < 0.01) in this group. Negative correlations between total antioxidant capacity and total oxidant status and total antioxidant capacity and malondialdehyde, and a positive correlation between total oxidant status and malondialdehyde were found in infected sheep.Conclusion:The obtained results indicated the relationship between oxidant/antioxidant imbalance andPsoroptes ovisinfection in sheep. Their MDA, TAC, TOS, and OSI markers may be used to determine the oxidative stress in natural infections withPsoroptes ovis.

scholarly journals Oxidant and antioxidant parameters in people who fast during Ramadan, and those who do not

2019 ◽  
Vol 19 (3) ◽  
pp. 2713-2717
Author(s):  
Hasan Karsen ◽  
Emine Ayca Güler ◽  
İrfan Binici ◽  
Huseyin Taşkıran ◽  
Suleyman Yıldırım ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorimetric Method ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Blood Samples ◽  
Oxidative Stress Index ◽  
Antioxidant Parameters ◽  
Anti Oxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

Background: Objectives: The main aim of this study was to compare, by a new automated colorimetric method, oxidant and anti-oxidant status of those who fast during Ramadan and those who do not. For this purpose, total oxidant status (TOS), total anti-oxidant capacity (TAC) levels and oxidative stress index (OSI) were analysed. By doing this, we hope to examine whether fasting has any beneficial effect on human health.Materials and methods: Fifty seven people were included in this study. Of these, 30 people had been fasting during the month of Ramadan for at least ten years, but 27 had never fasted in their life. On the 15th day of Ramadan, blood samples were obtained from both groups after 12 hours of fasting. Results: The TAC levels of the fasting group (1.29±0,19) was quite higher than the non-fasting group (1,09±0,16) [EIC2][ICD3][ICD4](p< 0.001). Similarly, in the fasting group TOS level and the OSI (respectively 12,77±2,23 and 1,01±0,25) was quite lower [EIC5][ICD6][ICD7] than in the non-fasting group (respectively 14,15±2,04 and 1,33±0,30), (p<=0.019 and p<0.001, respectively).These findings show that the TOS level of non-fasting group was high. This oxidative stress might cause various illnesses. Therefore, fasting could play a significant part in health-protection by increasing total anti-oxidant capacity.Keywords: Fasting Ramadan, oxidative parameters.

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