Quantitative ultrasound assessment of the effect of parity on bone mineral density in females

Abstract Background The effect of pregnancy and breastfeeding on a female’s bone mineral density (BMD) is controversial. This prospective study aims to investigate the effect of parity on BMD among pre-menopausal multiparous females using quantitative ultrasound as a screening method and females with no pregnancies (nulliparous) as a control group. Methods A portable ultrasound-based bone densitometer (DMS PEGASUS SMART, Mauguio, France) was used to indirectly assess the BMD in 51 multiparous (29–45 years) and 51 nulliparous Arabic females (18–35 years) by quantifying the broadband ultrasound attenuation (BUA) from their right calcaneus bone. BUA > 70 db/mhz = normal, BUA 65–69.9 db/mhz = below average, BUA 55–64.9 db/mhz = osteopenia and BUA < 55 db/mhz = osteoporosis. Results There was a significant difference in mean BUA between multiparous and nulliparous females (74.1 db/mhz vs. 69.3 db/mhz, p = 0.006). The prevalence of normal BMD was significantly higher in the nulliparous group than in the multiparous group (70.6% vs. 47.1%, p = 0.02). Osteoporosis was found in the multiparous group only (3/51). Among the multiparous females who breastfed (43/51), a total of 51.2% (22/43) had normal BMD, 25.6% (11/43) had BMD below average, 18.6% (8/43) had osteopenia and 4.7% (2/43) had osteoporosis. No significant differences in mean BUA (p = 0.2) were found between the group of females who breastfed for one year (13/43; BUA: 70.5 ± 9.4), the group of females who breastfed for 6–11 months (8/43; BUA: 70.6 ± 10.0) and those who breastfed for less than six months (22/43; BUA: 71.6 ± 9.4). A binary logistic regression model built for predicting BMD normality showed significance for the variable parity (p = 0.03), while the effect of the possible confounding variables BMI and age on BMD normality was found to be non- significant (p = 0.1 and p = 0.6, respectively). Conclusion Parity affects the BMD, as assessed by a portable ultrasound-based bone densitometer, of young and middle-aged females as compared to the BMD of nulliparous females.

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Overexpression of Mig-6 in Cartilage Induces an Osteoarthritis-Like Phenotype in Mice

10.21203/rs.2.24144/v2 ◽

2020 ◽

Author(s):

Melina Bellini ◽

Michael Andrew Pest ◽

Manuela Miranda Rodrigues ◽

Ling Qin ◽

Jae-Wook Jeong ◽

...

Keyword(s):

Bone Mineral Density ◽

Body Composition ◽

Articular Cartilage ◽

Bone Mineral ◽

Cartilage Degeneration ◽

Negative Regulator ◽

Control Group ◽

Multiple Time ◽

Mineral Density ◽

Significant Difference

Abstract Background: Osteoarthritis (OA) is the most common form of arthritis and characterized by degeneration of articular cartilage. Mitogen-inducible gene 6 (Mig-6) has been identified as a negative regulator of the Epidermal Growth Factor Receptor (EGFR). Cartilage-specific Mig-6 knockout (KO) mice display increased EGFR signaling, an anabolic buildup of articular cartilage and formation of chondro-osseous nodules. Since our understanding of the EGFR/Mig-6 network in cartilage remains incomplete, we characterized mice with cartilage-specific overexpression of Mig-6 in this study. Methods: Utilizing knee joints from cartilage-specific Mig-6 overexpressing (Mig-6over/over) mice (at multiple time points), we evaluated the articular cartilage using histology, immunohistochemical staining and semi-quantitative histopathological scoring (OARSI) at multiple ages. MicroCT analysis was employed to examine skeletal morphometry, body composition, and bone mineral density.Results: Our data show that cartilage-specific Mig-6 overexpression did not cause any major developmental abnormalities in articular cartilage, although Mig-6over/over mice have slightly shorter long bones compared to the control group. Moreover, there was no significant difference in bone mineral density and body composition in any of the groups. However, our results indicate that Mig-6over/over male mice show accelerated cartilage degeneration at 12 and 18 months of age. Immunohistochemistry for SOX9 demonstrated that the number of positively stained cells in Mig-6over/over mice was decreased relative to controls. Immunostaining for MMP13 appeared increased in areas of cartilage degeneration in Mig-6over/over mice. Moreover, staining for phospho-EGFR (Tyr-1173) and lubricin (PRG4) was decreased in the articular cartilage of Mig-6over/over mice. Conclusion: Overexpression of Mig-6 in articular cartilage causes no major developmental phenotype; however, these mice develop earlier OA during aging. These data demonstrate that Mig-6/EGFR pathways is critical for joint homeostasis and might present a promising therapeutic target for OA.

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Overexpression Of Mig-6 In Cartilage Induces An Osteoarthritis-like Phenotype In Mice

10.21203/rs.2.24144/v1 ◽

2020 ◽

Author(s):

Melina Bellini ◽

Michael Andrew Pest ◽

Manuela Miranda Rodrigues ◽

Ling Qin ◽

Jae-Wook Jeong ◽

...

Keyword(s):

Bone Mineral Density ◽

Body Composition ◽

Articular Cartilage ◽

Bone Mineral ◽

Cartilage Degeneration ◽

Negative Regulator ◽

Control Group ◽

Multiple Time ◽

Mineral Density ◽

Significant Difference

Abstract Background: Osteoarthritis (OA) is the most common form of arthritis and characterized by degeneration of articular cartilage. Mitogen-inducible gene 6 (Mig-6) has been identified as a negative regulator of the Epidermal Growth Factor Receptor (EGFR). Cartilage-specific Mig-6 knockout (KO) mice display increased EGFR signaling, an anabolic buildup of articular cartilage and formation of chondro-osseous nodules. Since our understanding of the EGFR/Mig-6 network in cartilage remains incomplete, we characterized mice with cartilage-specific overexpression of Mig-6 in this study. Methods : Utilizing knee joints from cartilage-specific Mig-6 overexpressing ( Mig- 6over/over ) mice (at multiple time points), we evaluated the articular cartilage using histology, immunohistochemical staining and semi-quantitative OARSI scoring at multiple ages. MicroCT analysis was employed to examine skeletal morphometry, body composition, and bone mineral density. Results: Our data show that cartilage-specific Mig-6 overexpression did not cause any major developmental abnormalities in articular cartilage, although Mig-6 over/over mice have slightly shorter long bones compared to the control group. Moreover, there was no significant difference in bone mineral density and body composition in any of the groups. However, our results indicate that Mig-6 over/over male mice show accelerated cartilage degeneration at 12 and 18 months of age. Immunohistochemistry for SOX9 demonstrated that the number of positively stained cells in Mig-6 over/over mice decreased relative to controls. Immunostaining for MMP13 staining is increased in areas of cartilage degeneration in Mig-6 over/over mice. Moreover, staining for phospho-EGFR (Tyr-1173) and lubricin (PRG4) was decreased in the articular cartilage of Mig-6 over/over mice. Conclusion: Overexpression of Mig-6 in articular cartilage causes no major developmental phenotype; however these mice develop earlier OA during aging. These data demonstrate that Mig-6/EGFR pathways is critical for joint homeostasis and might present a promising therapeutic target for OA.

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Clinical effect of Zoledronic Acid in the treatment of Senile Osteoporosis

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.36.7.1964 ◽

2020 ◽

Vol 36 (7) ◽

Author(s):

Li Kong ◽

Kai Zuo ◽

Long Ma

Keyword(s):

Bone Mineral Density ◽

Zoledronic Acid ◽

Bone Mineral ◽

Adverse Reactions ◽

Clinical Effect ◽

Control Group ◽

Observation Group ◽

Mineral Density ◽

Senile Osteoporosis ◽

Significant Difference

Objective: To investigate the clinical efficacy of zoledronic acid in the treatment of senile osteoporosis. Methods: One hundred and six cases of senile osteoporosis who visited to our hospital from August 2017 to December 2018 for treatment were selected and randomly divided into a control group and an observation group. The control group was treated with conventional therapy, while the observation group was treated with zoledronic acid in addition to the treatment of the control group. Bone mineral density, pain degree, therapeutic effect and adverse reactions of the two groups were compared. Results: The total effective rate of the observation group was 96.67%, higher than 80.00% of the control group (P<0.05); the bone mineral density of lumbar vertebrae, femoral neck and Ward’ area in the two groups increased after 6 months of treatment, and the bone mineral density of the observation group increased more than that of the control group (P<0.05); the pain degree of the observation group was lower than that of the control group after 6 months of treatment, and the difference was significant (P<0.05). There was no significant difference in the occurrence of adverse reactions between the two groups (P>0.05). Conclusion: Zoledronic acid is helpful to alleviate clinical symptoms, reduce the degree of bone pain, and promote the increase of bone mass, and has high safety in the treatment of senile osteoporosis, which is worth promotion. doi: https://doi.org/10.12669/pjms.36.7.1964 How to cite this:Kong L, Zuo K, Ma L. Clinical effect of Zoledronic Acid in the treatment of Senile Osteoporosis. Pak J Med Sci. 2020;36(7):1703-1707. doi: https://doi.org/10.12669/pjms.36.7.1964 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Peripheral and Central Bone Mineral Density in Charcot’s Neuroarthropathy Compared in Diabetic and Nondiabetic Populations

Journal of the American Podiatric Medical Association ◽

10.7547/1020213 ◽

2012 ◽

Vol 102 (3) ◽

pp. 213-222 ◽

Cited By ~ 7

Author(s):

Robert M. Greenhagen ◽

Dane K. Wukich ◽

Rachel H. Jung ◽

Vassilios Vardaxis ◽

Robert M. Yoho

Keyword(s):

Diabetes Mellitus ◽

Bone Mineral Density ◽

Bone Mineral ◽

Serum Vitamin ◽

Control Group ◽

Mineral Density ◽

X Ray ◽

Serum Vitamin D ◽

Significant Difference ◽

Patients With Diabetes

Background: This prospective study was performed to compare calcaneal and lumbar bone mineral density (BMD) in individuals with and without diabetes mellitus. We compared bone density with the time from onset of Charcot’s neuroarthropathy (CN) in patients with unilateral, nonoperative, reconstructive-stage CN. The final purpose was to investigate the role that sex, age, and serum vitamin D level may have in osseous recovery. Methods: Thirty-three individuals were divided into three groups: controls and patients with diabetes mellitus with and without CN. Peripheral instantaneous x-ray imaging and dual-energy x-ray absorptiometry were performed. Results: The calcaneal BMD of patients with diabetes mellitus and CN was lower than that of the control group (P < .01) but was not significantly lower than that of patients with diabetes mellitus alone. There was no statistically significant difference in lumbar T-scores between groups. Women demonstrated lower BMD than did men (P = .02), but patients 60 years and older did not demonstrate significantly lower BMD than did patients younger than 60 years (P = .135). A negative linear relationship was demonstrated between time and BMD in patients with CN. Conclusions: The results of this study suggest that lumbar BMD does not reflect peripheral BMD in patients with diabetes mellitus and reconstructive-stage CN. This study has clinical implications when reconstructive osseous surgery is planned in patients with CN. (J Am Podiatr Med Assoc 102(3): 213–222, 2012)

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The effect of different exercise programs on bone mineral density and physical function in women with osteoporosis: A randomized controlled trial

International Journal of Growth and Development ◽

10.25081/ijgd.2017.v1i1.48 ◽

2017 ◽

Vol 1 (1) ◽

pp. 47

Author(s):

Lumnije Hoxha Kamberi

Keyword(s):

Bone Mineral Density ◽

Muscle Strength ◽

Physical Function ◽

Postmenopausal Women ◽

Bone Mineral ◽

Controlled Trial ◽

Intervention Group ◽

Control Group ◽

Mineral Density ◽

Significant Difference

Aim: Osteoporosis is a multifactorial progressive skeletal disorder characterized by reduced bone mass. Exercise is widely recommended to reduce osteoporosis, falls and related fragility fractures. The purpose of this study was to investigate the effects of land exercise (LE) and aquatic exercise (AE) on physical function and bone mineral density (BMD). Methods: Fifty-eight postmenopausal women, aged 50-70 years, diagnosed with osteoporosis according to BMD measures, enrolled in this study. The subjects were randomly assigned to either the intervention group (LE group) or the control group (AE group). Physical function and BMD were assessed in all subjects in both groups before and after 10 months of intervention. The muscle strength, flexibility, balance, gait time and pain were measured to assess physical function. Bone mineral density at the lumbar spine was measured by dual energy X-ray absorptiometry (DEXA). Results: There were no significant differences between the two groups in the baseline anthropometric data. The two groups were similar with respect to age, weight, height, and body mass index (p>0.05). After the exercise program, muscle strength, flexibility, gait time, pain, and bone density (p<0.001) significantly improved with LE compared to AE. There was no significant difference between the two groups in balance at the 10-month follow-up. Conclusion: Significant improvements in physical function and BMD suggest that LE is a possible alternative for postmenopausal women with OP. Clinical rehabilitation impact: In the current available literature there is insufficient data regarding combined regimens, additionally, conclusions from our research can inspire further studies in order to promote land and water based exercise.

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THE RELATIONSHIP OF THE DISTAL DIABETIC POLYNEUROPATHY WITH PARAMETERS OF FOOT BONE MINERAL DENSITY IN PATIENTS WITH DIABETES MELLITUS

Osteoporosis and Bone Diseases ◽

10.14341/osteo2013114-17 ◽

2013 ◽

Vol 16 (1) ◽

pp. 14-17

Author(s):

N A Molitvoslovova ◽

T O Zernova ◽

M V Yaroslavtseva ◽

G R Galstyan

Keyword(s):

Diabetes Mellitus ◽

Bone Mineral Density ◽

Bone Mineral ◽

Diabetic Polyneuropathy ◽

Control Group ◽

Mineral Density ◽

Group 13 ◽

Significant Difference ◽

Patients With Diabetes ◽

Total Body

The aim of the study was to evaluate foot bone mineral density (BMD) in diabetes mellitus (DM) complicated with distal neuropathy (DN). Materials and methods. 61 patients with DM (DM1-27, DM2-34) were included. 37patients had Charcot osteoarthropathy (the 1st group), the 2nd group (13 patients) with severe DN, the 3rd group (11 patients) with mild DN, and control group consisted of 15 healthy people. All patients underwent dual energy X-ray absorptiometry (DXA) Lunar Prodigy scan. BMD was measured in lumbar spine, hip and radius. Foot BMD was measured using the «Total Body» region’s analysis. Results. There was a significant difference in foot BMD between controls and the 1st (р=0,031) and the 3rd (р=0,027) groups with no significant difference between the groups of patients. Foot BMD significantly correlated with spine, hip and radiusBMD (г=0,5-0,63, р<0,00001), BMI (r=0,4, р=0,000). Negative correlation was found between foot BMD and diabetes duration (r=-0,3, p<0,005) and HbA1c (r=-0,2, р=0,045). No correlation was found between DN and foot BMD. Conclusion. No association between severity of DN and foot BMD was found.

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Status of bone mineral content and body composition in boys engaged in intensive physical activity

Vojnosanitetski pregled ◽

10.2298/vsp1005386m ◽

2010 ◽

Vol 67 (5) ◽

pp. 386-390 ◽

Cited By ~ 4

Author(s):

Dejan Madic ◽

Borislav Obradovic ◽

Miroslav Smajic ◽

Jelena Obradovic ◽

Dusan Maric ◽

...

Keyword(s):

Physical Activity ◽

Bone Mineral Density ◽

Body Composition ◽

Bone Mineral ◽

Soccer Players ◽

Control Group ◽

Mineral Density ◽

Significant Difference ◽

Prepubertal Boys ◽

The Right

Background/Aim. It is well known that physical activity has an anabolic effect on bone tissue. But there is a lack of information about the effect of intensive physical activity in childhood, particularly at the prepubertal stage. To examine the influence of training on body composition and bone mineral density we have studied a group of prepubertal soccer players as well as a group of inactive prepubertal boys at the starting phase of their peak bone mass acquisition. Methods. A total of 62 healthy prepubertal boys took part in this study. They were divided into two groups. The first one consisted of 32 soccer players (aged 10.7 ? 0.5 years), who had been playing football for at least 1 year (10-15 h per week). The second group a control group 30 boys (aged 11.2 ? 0.7 years) doing 1.5 h per week physical activity at school. Body composition was assessed by a Body Fat Analyzer 'BES 200 Z'. Bone mineral density measurements of the left and the right calcaneus were done by using ultrasound densitometer 'Sahara' (Hologic, Inc., MA, USA). Results. There were significant differences between soccer players and the control group in fat mass (p = 0.01). Besides, a significant difference was determined between the group of athletes and the control group in bone mineral density of both calcaneal bones (p = 0.01). Conclusion. The results of this study confirm the significant effects of physical activity on reducing body mass and increasing bone density. Considering that football training can be very easily implemented in the broader population of children and young people, which does not apply to many other sports, it should be used more in the prevention of obesity and osteoporosis.

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Bone mineral changes during pregnancy: a cross-sectional study

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20192159 ◽

2019 ◽

Vol 8 (6) ◽

pp. 2172

Author(s):

Ritu Sharma ◽

Deepali Garg ◽

Huma Khan

Keyword(s):

Bone Mineral Density ◽

Bone Turnover ◽

Pregnant Women ◽

Bone Mineral ◽

Serum Calcium ◽

Bone Turnover Markers ◽

Control Group ◽

Mineral Density ◽

Significant Difference ◽

Turnover Markers

Background: Changes in bone mineral density during pregnancy are not widely studied because of the risk of radiation hazard to the fetus. But newer technology like DEXA has made it possible to measure bone density accurately with low dose radiations which are safe even during pregnancy. The aim of this study was to evaluate the changes in maternal bone turnover markers and bone mineral content at forearm during pregnancy.Methods: A total of 32 pregnant women with singleton pregnancy of more than thirty five weeks gestational age and thirty non-pregnant, non-lactating women as controls were recruited. Baseline blood investigations, serum calcium, serum alkaline phosphatase and DEXA of the forearm at ultra-distal, mid radius and proximal 1/3rd of radius were done at the time of recruitment into the study.Results: Bone mineral density of forearm of pregnancy group was compared with non-pregnant, non-lactating control group to see the effect of pregnancy. Bone turnover markers like serum calcium and serum ALP were also compared among pregnancy group and controls. The mean bone mineral density of controls at ultra-distal radius was 0.437±0.058g/cm2, while in pregnant women it was 0.431±0.58g/cm2 that was not statistically significant. Bone mineral density at mid radius in control was 0.599±0.051g/cm2 and in pregnant women it was 0.597±0.048 g/cm2 with no significant difference. BMD at proximal 1/3rd radius in controls was 0.670±0.36 g/cm2 as compared to pregnant women where it was 0.660±0.036 g/cm2 without any statistical significance. Total BMD at forearm in control and pregnant women was 0.586±0.035 and 0.582±0.036 respectively and again there was no statistically significant difference.Conclusions: There is no significant impact of pregnancy on bone mineral density of forearm. Although more bone areas need to be investigated for the further confirmation.

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AB0477 EFFECT OF ANTI-TNF THERAPY ON BONE MINERAL DENSITY IN ANKYLOSING SPONDYLORTHITIS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.2940 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 1266.1-1266

Author(s):

A. Ajerouassi ◽

K. Nassar ◽

S. Janani

Keyword(s):

Bone Mineral Density ◽

Femoral Neck ◽

Bone Mineral ◽

Spinal Mobility ◽

Tnf Alpha ◽

Control Group ◽

Mineral Density ◽

T Score ◽

Significant Difference ◽

The Mean

Background:Osteoporosis is common in spondyloarthritis, due to reduced spinal mobility, and inflammation. Anti-inflammatory treatments have a beneficial effect on the bone, and there is a significant increase in bone density during treatment with anti-TNF alpha.Objectives:to study bone mineral density in patients with ankylosing spondyloarthritis (AS) treated with anti-TNF alpha.Methods:This is a retrospective descriptive study of patients with AS meeting the modified New York criteria. Bone mineral density, assessed by dual energy x-ray absorptiometry (DXA), of AS patients treated with anti-TNF alpha was compared to that of a control group of AS patients not treated with anti-TNF alpha.Inclusion criteria:- Male patients- Patients who do not have an abnormality disrupting phosphocalcic and bone metabolism- For Patients on anti-TNF alpha: the treatment must be received for more than 6 monthsResults:A total of 22 patients were included, including 11 patients on anti-TNF alpha and 11 patients not on anti-TNF. The mean age (standard deviation) was 28 (± 7.2) years and 41 (± 14.8) in the cases and controls respectively. The mean body mass index in the AS group on anti-TNF was 22.16 kg / m2 and in the control group was 19.64 kg / m2. In the AS group on anti-TNF alpha, the mean bone mineral density of the spine was 1.092 g / cm2 (mean T score = -0.63) and that of the femoral neck, the mean bone mineral density was 0.888g / cm2 (mean T score = -1.04). In the control group, the mean bone mineral density of the spine was 0.959 g / cm2 (mean T score = -1.91) and the mean bone mineral density of the femoral neck was 0.774 g / cm2 (mean T score = -1.99). Bone mineral density in the spine and cervix was higher in the group receiving anti-TNF alpha (p = 0.09, p = 0.173 respectively)Conclusion:Our study shows the increase, although not statistically significant, in bone mineral density in AS patients receiving anti-TNF alpha agents compared to controls. Our results agree with those of the literature which support the bone protective effect of anti-TNF alpha. The non-significant difference can be explained by the delay in the introduction of biotherapy at the advanced stage of the structural evolution of AS. The best solution is to start TNF inhibitors at the early inflammatory stage of AS.Disclosure of Interests:None declared.

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Overexpression of mig-6 in cartilage induces an osteoarthritis-like phenotype in mice

10.1101/764142 ◽

2019 ◽

Cited By ~ 1

Author(s):

Melina Bellini ◽

Michael A. Pest ◽

M. Miranda-Rodrigues ◽

JW Jeong ◽

Frank Beier

Keyword(s):

Bone Mineral Density ◽

Body Composition ◽

Articular Cartilage ◽

Bone Mineral ◽

Cartilage Degeneration ◽

Negative Regulator ◽

Control Group ◽

Multiple Time ◽

Mineral Density ◽

Significant Difference

ABSTRACTBackgroundOsteoarthritis (OA) is the most common form of arthritis and characterized by degeneration of articular cartilage. Mitogen-inducible gene 6 (Mig-6) has been identified as a negative regulator of the Epidermal Growth Factor Receptor (EGFR). Cartilage-specific Mig-6 knockout (KO) mice display increased EGFR signaling, an anabolic buildup of articular cartilage and formation of chondro-osseous nodules. Since our understanding of the EGFR/Mig-6 network in cartilage remains incomplete, we characterized mice with cartilage-specific overexpression of Mig-6 in this study.MethodsUtilizing knee joints from cartilage-specific Mig-6 overexpressing (Mig-6over/over) mice (at multiple time points), we evaluated the articular cartilage using histology, immunohistochemical staining and semi-quantitative OARSI scoring at multiple ages. MicroCT analysis was employed to examine skeletal morphometry, body composition, and bone mineral density.ResultsOur data show that cartilage-specific Mig-6 overexpression did not cause any major developmental abnormalities in articular cartilage, although Mig-6over/over mice have slightly shorter long bones compared to the control group. Moreover, there was no significant difference in bone mineral density and body composition in any of the groups. However, our results indicate that Mig-6over/over male mice show accelerated cartilage degeneration at 12 and 18 months of age. Immunohistochemistry for SOX9 demonstrated that the number of positively stained cells in Mig-6over/over mice decreased relative to controls. Immunostaining for MMP13 staining is increased in areas of cartilage degeneration in Mig-6over/over mice. Moreover, staining for phospho-EGFR (Tyr-1173) and lubricin (PRG4) was decreased in the articular cartilage of Mig-6over/over mice.ConclusionOverexpression of Mig-6 in articular cartilage causes no major developmental phenotype; however these mice develop earlier OA during aging. These data demonstrate that Mig-6/EGFR pathways is critical for joint homeostasis and might present a promising therapeutic target for OA.

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