Effects of the Ayurved Siriraj Wattana recipe on functional and phenotypic characterization of cytokine-induced killer cells and dendritic cells in vitro

To investigate the biocontrol mechanisms by which the antagonistic Fusarium oxysporum strain Fo47 is active against Fusarium wilt, a Fot1 transposon-mediated insertional mutagenesis approach was adopted to generate mutants affected in their antagonistic activity. Ninety strains in which an active Fot1 copy had transposed were identified with a phenotypic assay for excision and tested for their biocontrol activity against F. oxysporum f. sp. lini on flax in greenhouse experiments. Sixteen strains were affected in their capacity to protect flax plants, either positively (more antagonistic than Fo47) or negatively (less antagonistic). The molecular characterization of these mutants confirms the excision of Fot1 and its reinsertion in most of the cases. Moreover, we demonstrate that other transposable elements such as Fot2, impala, and Hop have no transposition activity in the mutant genomes. The phenotypic characterization of these mutants shows that they are affected neither in their in vitro growth habit nor in their competitiveness in soil compared with wild-type strain Fo47. These results show that mutants are not impaired in their saprophytic phase and suggest that the altered biocontrol phenotype should likely be expressed during the interaction with the host plant.

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Phenotypic characterization of lymphokine-activated killer cells from human lymph node lymphocytes

Cellular Immunology ◽

10.1016/0008-8749(89)90104-4 ◽

1989 ◽

Vol 122 (2) ◽

pp. 578-584 ◽

Cited By ~ 9

Author(s):

Tokujiro Yano ◽

Mitsuhiro Murata ◽

Teruyoshi Ishida ◽

Tetsuya Mitsudomi ◽

Genki Kimura ◽

...

Keyword(s):

Lymph Node ◽

Phenotypic Characterization ◽

Lymphokine Activated Killer Cells ◽

Killer Cells ◽

Human Lymph Node

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Characterization of the defective interaction between a subset of natural killer cells and dendritic cells in HIV-1 infection

Journal of Experimental Medicine ◽

10.1084/jem.20060894 ◽

2006 ◽

Vol 203 (10) ◽

pp. 2339-2350 ◽

Cited By ~ 129

Author(s):

Domenico Mavilio ◽

Gabriella Lombardo ◽

Audrey Kinter ◽

Manuela Fogli ◽

Andrea La Sala ◽

...

Keyword(s):

Dendritic Cells ◽

Nk Cells ◽

Natural Killer ◽

Nk Cell ◽

Cell Subset ◽

Interferon Γ ◽

And Function ◽

Hiv 1

In this study, we demonstrate that the in vitro interactions between a CD56neg/CD16pos (CD56neg) subset of natural killer (NK) cells and autologous dendritic cells (DCs) from HIV-1–infected viremic but not aviremic individuals are markedly impaired and likely interfere with the development of an effective immune response. Among the defective interactions are abnormalities in the process of reciprocal NK–DC activation and maturation as well as a defect in the NK cell–mediated editing or elimination of immature DCs (iDCs). Notably, the lysis of mature DCs (mDCs) by autologous NK cells was highly impaired even after the complete masking of major histocompatibility complex I molecules, suggesting that the defective elimination of autologous iDCs is at the level of activating NK cell receptors. In this regard, the markedly impaired expression/secretion and function of NKp30 and TNF-related apoptosis-inducing ligand, particularly among the CD56neg NK cell subset, largely accounts for the highly defective NK cell–mediated lysis of autologous iDCs. Moreover, mDCs generated from HIV-1 viremic but not aviremic patients are substantially impaired in their ability to secrete interleukin (IL)-10 and -12 and to prime the proliferation of neighboring autologous NK cells, which, in turn, fail to secrete adequate amounts of interferon-γ.

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Construction and Phenotypic Characterization of an Auxotrophic Mutant of Mycobacterium tuberculosis Defective in l-Arginine Biosynthesis

Infection and Immunity ◽

10.1128/iai.70.6.3080-3084.2002 ◽

2002 ◽

Vol 70 (6) ◽

pp. 3080-3084 ◽

Cited By ~ 55

Author(s):

Bhavna G. Gordhan ◽

Debbie A. Smith ◽

Heidi Alderton ◽

Ruth A. McAdam ◽

Gregory J. Bancroft ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Mutant Strain ◽

Auxotrophic Mutant ◽

Phenotypic Characterization ◽

Wild Type ◽

Arginine Biosynthesis ◽

High Concentrations

ABSTRACT A mutant of Mycobacterium tuberculosis defective in the metabolism of l-arginine was constructed by allelic exchange mutagenesis. The argF mutant strain required exogenous l-arginine for growth in vitro, and in the presence of 0.96 mM l-arginine, it achieved a growth rate and cell density in stationary phase comparable to those of the wild type. The mutant strain was also able to grow in the presence of high concentrations of argininosuccinate, but its auxotrophic phenotype could not be rescued by l-citrulline, suggesting that the ΔargF::hyg mutation exerted a polar effect on the downstream argG gene but not on argH. The mutant strain displayed reduced virulence in immunodeficient SCID mice and was highly attenuated in immunocompetent DBA/2 mice, suggesting that l-arginine availability is restricted in vivo.

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Characterization of the Phosphodiesterase (PDE) Pattern of in Vitro-Generated Human Dendritic Cells (DC) and the Influence of PDE Inhibitors on DC Function

Pulmonary Pharmacology & Therapeutics ◽

10.1006/pupt.1999.0220 ◽

1999 ◽

Vol 12 (6) ◽

pp. 377-386 ◽

Cited By ~ 31

Author(s):

Florian Gantner ◽

Christian Schudt ◽

Albrecht Wendel ◽

Armin Hatzelmann

Keyword(s):

Dendritic Cells ◽

Dc Function ◽

Pde Inhibitors ◽

Human Dendritic Cells

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A new strategy using autologous dendritic cells and lymphokine-activated killer cells for cancer immunotherapy: efficient maturation of DCs by co-culture with LAK cells in vitro

Oncology Reports ◽

10.3892/or.16.1.147 ◽

2006 ◽

Author(s):

Yutaro Yano ◽

Yuji Ueda ◽

Tsuyohi Itoh ◽

Nobuaki Fuji ◽

Kaori Okugawa ◽

...

Keyword(s):

Dendritic Cells ◽

Cancer Immunotherapy ◽

Lak Cells ◽

Lymphokine Activated Killer Cells ◽

Killer Cells ◽

New Strategy

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Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8α+ dendritic cells

Journal of Experimental Medicine ◽

10.1084/jem.20092618 ◽

2010 ◽

Vol 207 (6) ◽

pp. 1261-1271 ◽

Cited By ~ 471

Author(s):

Lionel Franz Poulin ◽

Mariolina Salio ◽

Emmanuel Griessinger ◽

Fernando Anjos-Afonso ◽

Ligia Craciun ◽

...

Keyword(s):

Dendritic Cells ◽

T Cell ◽

Poly I:C ◽

Clinical Protocols ◽

Cell Responses ◽

Promising Target ◽

Important Player ◽

And Function

In mouse, a subset of dendritic cells (DCs) known as CD8α+ DCs has emerged as an important player in the regulation of T cell responses and a promising target in vaccination strategies. However, translation into clinical protocols has been hampered by the failure to identify CD8α+ DCs in humans. Here, we characterize a population of human DCs that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8α+ DCs in phenotype and function. We describe the presence of such cells in the spleens of humans and humanized mice and report on a protocol to generate them in vitro. Like mouse CD8α+ DCs, human DNGR-1+ BDCA3hi DCs express Necl2, CD207, BATF3, IRF8, and TLR3, but not CD11b, IRF4, TLR7, or (unlike CD8α+ DCs) TLR9. DNGR-1+ BDCA3hi DCs respond to poly I:C and agonists of TLR8, but not of TLR7, and produce interleukin (IL)-12 when given innate and T cell–derived signals. Notably, DNGR-1+ BDCA3+ DCs from in vitro cultures efficiently internalize material from dead cells and can cross-present exogenous antigens to CD8+ T cells upon treatment with poly I:C. The characterization of human DNGR-1+ BDCA3hi DCs and the ability to grow them in vitro opens the door for exploiting this subset in immunotherapy.

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