Harnessing Versatile Dynamic Carbon Precursors for Multi-Color Emissive Carbon Dots

Carbon dots (CDs), a relatively new kind of fluorescent nanomaterials superior to other contributors of carbon allotrope due to their excellent biocompatibility, controllable photoluminescence, high quantum yield (QY), unique electronic...

Incorporating Fluorescent Nanomaterials in Organically Modified Sol-Gel Materials – Creating Single Composite Optical pH Sensors

Optical sensors hold the promise of providing the coupling between the tangible and the digital world that we are currently experiencing with physical sensors. The core of optical sensor development...

Advances and Challenges of Fluorescent Nanomaterials for Synthesis and Biomedical Applications

With the rapid development of nanotechnology, new types of fluorescent nanomaterials (FNMs) have been springing up in the past two decades. The nanometer scale endows FNMs with unique optical properties which play a critical role in their applications in bioimaging and fluorescence-dependent detections. However, since low selectivity as well as low photoluminescence efficiency of fluorescent nanomaterials hinders their applications in imaging and detection to some extent, scientists are still in search of synthesizing new FNMs with better properties. In this review, a variety of fluorescent nanoparticles are summarized including semiconductor quantum dots, carbon dots, carbon nanoparticles, carbon nanotubes, graphene-based nanomaterials, noble metal nanoparticles, silica nanoparticles, phosphors and organic frameworks. We highlight the recent advances of the latest developments in the synthesis of FNMs and their applications in the biomedical field in recent years. Furthermore, the main theories, methods, and limitations of the synthesis and applications of FNMs have been reviewed and discussed. In addition, challenges in synthesis and biomedical applications are systematically summarized as well. The future directions and perspectives of FNMs in clinical applications are also presented.

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

Ubiquitous on Earth, DNA and other nucleic acids are being increasingly considered as promising biomass resources. Due to their unique chemical structure, which is different from that of more common carbohydrate biomass polymers, materials based on nucleic acids may exhibit new, attractive characteristics. In this study, fluorescent nanoparticles (biodots) were prepared by a hydrothermal (HT) method from various nucleic acids (DNA, RNA, nucleotides, and nucleosides) to establish the relationship between the structure of precursors and fluorescent properties of biodots and to optimize conditions for preparation of the most fluorescent product. HT treatment of nucleic acids results in decomposition of sugar moieties and depurination/depyrimidation of nucleobases, while their consequent condensation and polymerization gives fluorescent nanoparticles. Fluorescent properties of DNA and RNA biodots are drastically different from biodots synthesized from individual nucleotides. In particular, biodots synthesized from purine-containing nucleotides or nucleosides show up to 50-fold higher fluorescence compared to analogous pyrimidine-derived biodots. The polymeric nature of a precursor disfavors formation of a bright fluorescent product. The reported effect of the structure of the nucleic acid precursor on the fluorescence properties of biodots should help designing and synthesizing brighter fluorescent nanomaterials with broader specification for bioimaging, sensing, and other applications.

Efficient Inhibition of Streptococcus agalactiae by AIEgen-Based Fluorescent Nanomaterials

Streptococcus agalactiae, referred to as group B streptococcus (GBS), is a prominent co-pathogenic bacterium causing the onset and death of human, animal, and aquatic products. Although antibiotics are efficient against GBS, antibiotic resistance through antibiotic overuse is an equally serious problem. Therefore, the treatment of GBS infection appears strongly dependent on nonantibiotic therapy, such as photodynamic therapy. Different from other photosensitizers (PSs), luminogens with aggregation-induced emission (AIEgen) can efficiently generate fluorescence and reactive oxygen species (ROS). Herein, TBP-1, an efficient AIE PSs, is chosen to resist GBS, and its antibacterial activity and the killing mechanism toward GBS are investigated. The ROS generation performance and the images of GBS treated with TBP-1 in the dark or under white light irradiation were investigated. TBP-1 with its high ROS generation ability can efficiently kill GBS and serve as a novel treatment strategy against GBS infection.

Precursor-dependent structural diversity in luminescent carbonized polymer dots (CPDs): the nomenclature

Carbon dots (CDs) have received immense attention in the last decade because they are easy-to-prepare, nontoxic, and tailorable carbon-based fluorescent nanomaterials. CDs can be categorized into three subgroups based on their morphology and chemical structure: graphene quantum dots (GQDs), carbon quantum dots (CQDs), and carbonized polymer dots (CPDs). The detailed structures of the materials can vary significantly, even within the same category. This property is particularly predominant in chemically synthesized CPDs, as their formation proceeds via the polymerization–carbonization of molecules or polymer precursors. Abundant precursors endow CPDs with versatile structures and properties. A wide variety of carbon nanomaterials can be grouped under the category of CPDs because of their observed diversity. It is important to understand the precursor-dependent structural diversity observed in CPDs. Appropriate nomenclature for all classes and types of CPDs is proposed for the better utilization of these emerging materials.

Influence of Chitosan-Based Carbon Dots on Astaxanthin Production of Green Alga Tetraselmis sp.

CDs are carbon fluorescent nanomaterials that have gained significant attention in recent years owing to their unique properties. In this work, we utilized a simple solution to produce CDs with func-tionalized amino groups via a one-step carbonization from a chitosan precursor. This simultaneous approach does not use special reagent for either the formation step or the amino-functionalization step of CDs. The as-prepared amino-functionalized CDs that possesses expected characteristics, such as nano-size distribution, monodispersible, high blue light emission, high absolute quantum yield of 5.52%, and functionalized amino groups on the surface. Furthermore, this work demonstrated the low cytotoxicity and high biocompatibility of the CDs, through the improvements in the astaxanthin production of alga Tetraselmis sp. (more than doubled (up to 0.044 mg/L), relative to the control). Thus, as-prepared CDs have promising properties not only for applications in bioimaging, drug delivery or sensors, but also as promoter in algal biorefinery