scholarly journals Endogenous microRNA triggered enzyme-free DNA logic self-assembly for amplified bioimaging and enhanced gene therapy via in situ generation of siRNAs

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Qinghua Jiang ◽  
Shuzhen Yue ◽  
Kaixin Yu ◽  
Tian Tian ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cervical Carcinoma ◽  
Self Assembly ◽  
Resonance Energy ◽  
Dna Amplification ◽  
High Sensitivity ◽  
Free Dna ◽  
Therapy Effect ◽  
In Situ Generation

Abstract Background Small interfering RNA (siRNA) has emerged as a kind of promising therapeutic agents for cancer therapy. However, the off-target effect and degradation are the main challenges for siRNAs delivery. Herein, an enzyme-free DNA amplification strategy initiated by a specific endogenous microRNA has been developed for in situ generation of siRNAs with enhanced gene therapy effect on cervical carcinoma. Methods This strategy contains three DNA hairpins (H1, H2/PS and H3) which can be triggered by microRNA-21 (miR-21) for self-assembly of DNA nanowheels (DNWs). Notably, this system is consistent with the operation of a DNA logic circuitry containing cascaded “AND” gates with feedback mechanism. Accordingly, a versatile biosensing and bioimaging platform is fabricated for sensitive and specific analysis of miR-21 in HeLa cells via fluorescence resonance energy transfer (FRET). Meanwhile, since the vascular endothelial growth factor (VEGF) antisense and sense sequences are encoded in hairpin reactants, the performance of this DNA circuit leads to in situ assembly of VEGF siRNAs in DNWs, which can be specifically recognized and cleaved by Dicer for gene therapy of cervical carcinoma. Results The proposed isothermal amplification approach exhibits high sensitivity for miR-21 with a detection limit of 0.25 pM and indicates excellent specificity to discriminate target miR-21 from the single-base mismatched sequence. Furthermore, this strategy achieves accurate and sensitive imaging analysis of the expression and distribution of miR-21 in different living cells. To note, compared to naked siRNAs alone, in situ siRNA generation shows a significantly enhanced gene silencing and anti-tumor effect due to the high reaction efficiency of DNA circuit and improved delivery stability of siRNAs. Conclusions The endogenous miRNA-activated DNA circuit provides an exciting opportunity to construct a general nanoplatform for precise cancer diagnosis and efficient gene therapy, which has an important significance in clinical translation. Graphic abstract

scholarly journals Endogenous microRNA Triggered Enzyme-free DNA Logic Self-assembly for Amplified Bioimaging and Enhanced Gene Therapy via in Situ Generation of siRNAs

2021 ◽  
Author(s):  
Qinghua Jiang ◽  
Shuzhen Yue ◽  
Kaixin Yu ◽  
Tian Tian ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Self Assembly ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Amplification ◽  
High Sensitivity ◽  
Free Dna ◽  
Therapy Effect ◽  
In Situ Generation

Abstract BackgroundSmall interfering RNA (siRNA) has emerged as a kind of promising therapeutic agents for cancer therapy. However, the off-target effect and degradation are the main challenges for siRNAs delivery. Herein, an enzyme-free DNA amplification strategy initiated by a specific endogenous microRNA has been developed for in situ generation of siRNAs with enhanced gene therapy effect on cervical carcinoma.MethodsThis strategy contains three DNA hairpins (H1, H2/PS and H3) which can be triggered by microRNA-21 (miR-21) for self-assembly of DNA nanowheels (DNWs). Notably, this system is consistent with the operation of a DNA logic circuitry containing cascaded “AND” gates with feedback mechanism. Accordingly, a versatile biosensing and bioimaging platform is fabricated for sensitive and specific analysis of miR-21 in HeLa cells via fluorescence resonance energy transfer (FRET). Meanwhile, since the vascular endothelial growth factor (VEGF) antisense and sense sequences are encoded in hairpin reactants, the performance of this DNA circuit leads to in situ assembly of VEGF siRNAs in DNWs, which can be specifically recognized and cleaved by Dicer for gene therapy of cervical carcinoma. ResultsThe proposed isothermal amplification approach exhibits high sensitivity for miR-21 with a detection limit of 0.25 pM and indicates excellent specificity to discriminate target miR-21 from the single-base mismatched sequence. Furthermore, this strategy achieves accurate and sensitive imaging analysis of the expression and distribution of miR-21 in different living cells. To note, compared to naked siRNAs alone, in situ siRNA generation shows a significantly enhanced gene silencing and anti-tumor effect due to the high reaction efficiency of DNA circuit and improved delivery stability of siRNAs.ConclusionThe endogenous miRNA-activated DNA circuit provides an exciting opportunity to construct a general nanoplatform for precise cancer diagnosis and efficient gene therapy, which has an important significance in clinical translation.

scholarly journals An enzyme-free molecular catalytic device: dynamically self-assembled DNA dendrimers for in situ imaging of microRNAs in live cells

2019 ◽  
Vol 10 (6) ◽  
pp. 1651-1658 ◽  
Author(s):  
Shuzhen Yue ◽  
Xinyue Song ◽  
Weiling Song ◽  
Sai Bi
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Live Cells ◽  
Sensitivity And Selectivity ◽  
Catalytic Hairpin Assembly ◽  
Self Assembled ◽  
In Situ Imaging

Branched catalytic hairpin assembly is proposed for dynamic self-assembly of DNA dendrimers for signal amplification and in situ imaging of microRNAs in live cells with high sensitivity and selectivity.

In Situ Generation and Consumption of H2O2 by Bienzyme–Quantum Dots Bioconjugates for Improved Chemiluminescence Resonance Energy Transfer

2016 ◽  
Vol 88 (12) ◽  
pp. 6418-6424 ◽  
Author(s):  
Shuxia Xu ◽  
Xianming Li ◽  
Chaobi Li ◽  
Jialin Li ◽  
Xinfeng Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
In Situ Generation

Vesicular self-assembly of a natural triterpenoid arjunolic acid in aqueous medium: study of entrapment properties and in situ generation of gel–gold nanoparticle hybrid material

RSC Advances ◽  
2014 ◽  
Vol 4 (95) ◽  
pp. 53327-53334 ◽  
Author(s):  
Braja Gopal Bag ◽  
Rakhi Majumdar
Keyword(s):  
Aqueous Medium ◽  
Gold Nanoparticle ◽  
Hybrid Material ◽  
Self Assembly ◽  
Medium Study ◽  
Terminalia Arjuna ◽  
Saw Dust ◽  
Arjunolic Acid ◽  
In Situ Generation

Formation of vesicular gel and gel–gold nanoparticle hybrid material from arjunolic acid extractable from the saw-dust of Terminalia arjuna.

Selected-area in situ generation of highly fluorescent organic nanowires embedded in a polymer film: the solvent-vapor-induced self-assembly process

2010 ◽  
Vol 20 (36) ◽  
pp. 7715 ◽  
Author(s):  
Jong Won Chung ◽  
Byeong-Kwan An ◽  
Fumio Hirato ◽  
Jong H. Kim ◽  
Hiroshi Jinnai ◽  
...  
Keyword(s):  
Polymer Film ◽  
Self Assembly ◽  
Assembly Process ◽  
Solvent Vapor ◽  
In Situ Generation

In situ generation of plasmonic cavities for high sensitivity fluorophore and biomolecule detection

Nanoscale ◽  
2018 ◽  
Vol 10 (39) ◽  
pp. 18555-18564
Author(s):  
Daragh Byrne ◽  
Colette McDonagh
Keyword(s):  
High Sensitivity ◽  
Biomolecule Detection ◽  
In Situ Generation

Plasmonic cavities are grown in situ via Au labelled antibodies yielding a highly responsive fluorescence based biosensing platform.

scholarly journals Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1643 ◽  
Author(s):  
Yue-liang Zhai ◽  
Qiu-bo Wang ◽  
Hao Yu ◽  
Xiao-yuan Ji ◽  
Xian Zhang
Keyword(s):  
Fluorescence Imaging ◽  
Cross Sections ◽  
Self Assembly ◽  
Calcium Ion ◽  
Conjugated Polymer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Cellular Functions ◽  
Two Photon

The calcium ion (Ca2+) isa highly versatile intracellular signal messenger regulating many different cellular functions. It is important to design probes with good fluorescence and two-photon (TP) active cross-sections (Φδ) to explore the concentration distribution of Ca2+. In this manuscript, a novel TP fluorescence calcium probe (BAPTAVP) with positive charges, based on the classical Ca2+ indicator of BAPTA (1,2-bis(2-aminophenoxy)-ethane-N,N,N’,N’-tetra acetic acid), and a conjugated polymer (PCBMB) with negative charges were designed and synthesized. The results from transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), and the zeta potential (ZP) showed that nanoparticles were obtained by the self-assembly of PCBMB and BAPTAVP. Moreover, the fluorescence properties of BAPTAVP were effectively improved by fluorescence resonance energy transfer (FRET) with PCBMB and attenuating the intramolecular charge transfer (ICT) after the addition of Ca2+. The quantum yield and Φδ of PCBMB-BAPTAVP increased by about four and six times in comparison to those of BAPTAVP, respectively. The TP fluorescence imaging experiments indicated that the PCBMB-BAPTAVP system could effectively detect Ca2+ in living cells with high sensitivity.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Highly diastereo- and enantioselective organocatalytic synthesis of trifluoromethylated erythritols based on the in situ generation of unstable trifluoroacetaldehyde

2021 ◽  
Author(s):  
Kazumasa Funabiki ◽  
Toshiya Gotoh ◽  
Ryunosuke Kani ◽  
Toshiyasu Inuzuka ◽  
Yasuhiro Kubota
Keyword(s):  
Trifluoromethyl Group ◽  
In Situ Generation

A highly diastereo- and enantioselective organocatalytic method to synthesise erythritols bearing a trifluoromethyl group has been investigated.

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