scholarly journals Whole genome sequence comparison of ten diagnostic brucellaphages propagated on two Brucella abortus hosts

2015 ◽  
Vol 12 (1) ◽  
Author(s):  
Ekaterine Tevdoradze ◽  
Jason Farlow ◽  
Adam Kotorashvili ◽  
Natia Skhirtladze ◽  
Irina Antadze ◽  
...  
PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0123298 ◽  
Author(s):  
Markus H. Antwerpen ◽  
Karola Prior ◽  
Alexander Mellmann ◽  
Sebastian Höppner ◽  
Wolf D. Splettstoesser ◽  
...  

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 574 ◽  
Author(s):  
Laura Grande ◽  
Valeria Michelacci ◽  
Rosangela Tozzoli ◽  
Paola Ranieri ◽  
Antonella Maugliani ◽  
...  

2021 ◽  
Vol 9 (3) ◽  
pp. 570
Author(s):  
Maphuti Betty Ledwaba ◽  
Barbara Akorfa Glover ◽  
Itumeleng Matle ◽  
Giuseppe Profiti ◽  
Pier Luigi Martelli ◽  
...  

The availability of whole genome sequences in public databases permits genome-wide comparative studies of various bacterial species. Whole genome sequence-single nucleotide polymorphisms (WGS-SNP) analysis has been used in recent studies and allows the discrimination of various Brucella species and strains. In the present study, 13 Brucella spp. strains from cattle of various locations in provinces of South Africa were typed and discriminated. WGS-SNP analysis indicated a maximum pairwise distance ranging from 4 to 77 single nucleotide polymorphisms (SNPs) between the South African Brucella abortus virulent field strains. Moreover, it was shown that the South African B. abortus strains grouped closely to B. abortus strains from Mozambique and Zimbabwe, as well as other Eurasian countries, such as Portugal and India. WGS-SNP analysis of South African B. abortus strains demonstrated that the same genotype circulated in one farm (Farm 1), whereas another farm (Farm 2) in the same province had two different genotypes. This indicated that brucellosis in South Africa spreads within the herd on some farms, whereas the introduction of infected animals is the mode of transmission on other farms. Three B. abortus vaccine S19 strains isolated from tissue and aborted material were identical, even though they originated from different herds and regions of South Africa. This might be due to the incorrect vaccination of animals older than the recommended age of 4–8 months or might be a problem associated with vaccine production.


2014 ◽  
Vol 40 (12) ◽  
pp. 2059
Author(s):  
Lin-Yi QIAO ◽  
Xin LI ◽  
Zhi-Jian CHANG ◽  
Xiao-Jun ZHANG ◽  
Hai-Xian ZHAN ◽  
...  

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