Genotype-specific and cross-reactive neutralizing antibodies induced by dengue virus infection: detection of antibodies with different levels of neutralizing activities against homologous and heterologous genotypes of dengue virus type 2 in common marmosets (Callithrix jacchus)

ABSTRACT In 2015, a female patient returning to Australia from Sabah, Malaysia, was diagnosed with a suspected sylvatic dengue virus type 2 (DENV-2) infection, becoming the second case of imported highly divergent dengue virus infection recorded in Australia. We describe here the complete genome sequencing of the DENV-2 strain isolated from this patient.

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An outbreak of dengue virus (DENV) type 2 Cosmopolitan genotype in Israeli travellers returning from the Seychelles, April 2017

Eurosurveillance ◽

10.2807/1560-7917.es.2017.22.26.30563 ◽

2017 ◽

Vol 22 (26) ◽

Cited By ~ 6

Author(s):

Yaniv Lustig ◽

Dana Wolf ◽

Ora Halutz ◽

Eli Schwartz

Keyword(s):

Phylogenetic Analysis ◽

Virus Infection ◽

Dengue Virus ◽

Virus Type ◽

Dengue Virus Infection ◽

Diagnostic Capabilities ◽

Dengue Virus Type 2

Dengue virus infection was diagnosed in six Israeli travellers returning from the Seychelles in April 2017. Phylogenetic analysis identified identical sequences belonging to the Cosmopolitan genotype of dengue virus type 2 in all samples sequenced, thus providing evidence for a probable dengue type 2 outbreak in the Seychelles. This report further demonstrates the role of travellers as sentinels for arboviral infections, especially in countries with limited diagnostic capabilities.

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Induction of neutralizing antibodies and T cell responses by dengue virus type 2 envelope domain III encoded by plasmid and adenoviral vectors

Vaccine ◽

10.1016/j.vaccine.2006.06.031 ◽

2006 ◽

Vol 24 (42-43) ◽

pp. 6513-6525 ◽

Cited By ~ 33

Author(s):

Saima Khanam ◽

Navin Khanna ◽

Sathyamangalam Swaminathan

Keyword(s):

T Cell ◽

Dengue Virus ◽

Virus Type ◽

Neutralizing Antibodies ◽

T Cell Responses ◽

Adenoviral Vectors ◽

Cell Responses ◽

Domain Iii ◽

Dengue Virus Type 2

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Replication-Defective Adenoviral Vaccine Vector for the Induction of Immune Responses to Dengue Virus Type 2

Journal of Virology ◽

10.1128/jvi.77.23.12907-12913.2003 ◽

2003 ◽

Vol 77 (23) ◽

pp. 12907-12913 ◽

Cited By ~ 34

Author(s):

Smita Jaiswal ◽

Navin Khanna ◽

S. Swaminathan

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Neutralizing Antibodies ◽

Adenovirus Vector ◽

Interleukin 4 ◽

Promising Alternative ◽

Infected Cells ◽

Dengue Virus Type 2

ABSTRACT A recombinant replication-defective adenovirus vector that can overexpress the ectodomain of the envelope protein of dengue virus type 2 (NGC strain) has been constructed. This virus was immunogenic in mice and elicited dengue virus type 2 specific B- and T-cell responses. Sera from immunized mice contained neutralizing antibodies that could specifically recognize dengue virus type 2 and neutralize its infectivity in vitro, indicating that this approach has the potential to confer protective immunity. In vitro stimulation of splenocytes (from immunized mice) with dengue virus type 2 resulted in a significant proliferative response accompanied by the production of high levels of gamma interferon but did not show significant changes in interleukin-4 levels. This is suggestive of a Th1-like response (considered to be important in the maturation of cytotoxic T lymphocytes that are essential for the elimination of virus-infected cells). The data show that adenovirus vectors offer a promising alternative strategy for the development of dengue virus vaccines.

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