scholarly journals Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production, and its application in high-temperature fermentation

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Cleiton D. Prado ◽  
Gustavo P. L. Mandrujano ◽  
Jonas. P. Souza ◽  
Flávia B. Sgobbi ◽  
Hosana R. Novaes ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
High Temperature ◽  
Ethanol Production ◽  
Thermotolerant Yeast ◽  
Secondary Products ◽  
High Concentration ◽  
Physiological Characterization ◽  
Expression Of Genes ◽  
Thermotolerant Strain

Abstract Background The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This characteristic could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. Results We identified and characterized the physiology of a new thermotolerant strain (LBGA-01) able to ferment at 40 °C, which is more resistant to stressors as sucrose, furfural and ethanol than CAT-1 industrial strain. Furthermore, this strain showed similar CAT-1 resistance to acetic acid and lactic acid, and it was also able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). Genes related to the production of proteins involved in secondary products of fermentation were also differentially regulated at 40 °C, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-coenzyme A synthetase 2 (ACS2). Fermentation tests using chemostats showed that LBGA-01 had an excellent performance in ethanol production in high temperature. Conclusion The thermotolerant LBGA-01 strain modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its resistance to high concentration of ethanol, sugar, lactic acid, acetic acid, and furfural. Results indicate that this strain can be used to improve first- and second-generation ethanol production in Brazil.

scholarly journals Physiological characterization of a new thermotolerant yeast strain isolated during Brazilian ethanol production and its application in high temperature fermentation

2020 ◽  
Author(s):  
Cleiton Dias do Prado ◽  
Gustavo Patricio Lorca Mandrujano ◽  
Jonas Paulino de Souza ◽  
Flavia Beatriz Sgobbi ◽  
Hosana Ribeiro Novaes ◽  
...  
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Second Generation ◽  
Thermotolerant Yeast ◽  
Secondary Products ◽  
High Concentration ◽  
Physiological Characterization ◽  
Thermotolerant Strain ◽  
First And Second Generation ◽  
Second Generation Ethanol

Abstract Background The use of thermotolerant yeast strains can improve the efficiency of ethanol fermentation, allowing fermentation to occur at temperatures higher than 40 °C. This increment in temperature could benefit traditional bio-ethanol production and allow simultaneous saccharification and fermentation (SSF) of starch or lignocellulosic biomass. Results We identified and characterized the physiology of a new thermotolerant strain able to fermentate at 40 °C while producing high yields of ethanol. Our results showed that, in comparison to the industrial yeast CAT-1, our strain was more resistant to various stressors generated during the production of first- and second-generation ethanol, and it also was able to change the pattern of genes involved in sucrose assimilation (SUC2 and AGT1). The formation of secondary products of fermentation was different at 40ºC, with reduced expression of genes involved in the formation of glycerol (GPD2), acetate (ALD6 and ALD4), and acetyl-CoA (ACS2). Conclusion The LBGA-01 strain is a thermotolerant strain that modulates the production of key genes, changing metabolic pathways during high-temperature fermentation, and increasing its tolerance to the high concentration of ethanol, sugar, acetic lactic, acetic acid, furfural and HMF. This indicates that this strain can be used to improve first- and second-generation ethanol production in Brazil.

scholarly journals The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production

2018 ◽  
Vol 49 (2) ◽  
pp. 378-391 ◽  
Author(s):  
Nuttaporn Chamnipa ◽  
Sudarat Thanonkeo ◽  
Preekamol Klanrit ◽  
Pornthap Thanonkeo
Keyword(s):  
High Temperature ◽  
Ethanol Production ◽  
Thermotolerant Yeast ◽  
Pichia Kudriavzevii

scholarly journals l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

2021 ◽  
Vol 7 (11) ◽  
pp. 928
Author(s):  
Byeong-Kwan Jang ◽  
Yebin Ju ◽  
Deokyeol Jeong ◽  
Sung-Keun Jung ◽  
Chang-Kil Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acid Tolerance ◽  
Production Costs ◽  
High Concentration ◽  
Adaptive Laboratory Evolution ◽  
Engineered Saccharomyces Cerevisiae

Lactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered Saccharomyces cerevisiae can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to increased production costs. In this study, lactic acid-producing S. cerevisiae BK01 was developed with improved lactic acid tolerance through adaptive laboratory evolution (ALE) on 8% lactic acid. The genetic basis of BK01 could not be determined, suggesting complex mechanisms associated with lactic acid tolerance. However, BK01 had distinctive metabolomic traits clearly separated from the parental strain, and lactic acid production was improved by 17% (from 102 g/L to 119 g/L). To the best of our knowledge, this is the highest lactic acid titer produced by engineered S. cerevisiae without the use of pH neutralizers. Moreover, cellulosic lactic acid production by BK01 was demonstrated using acetate-rich buckwheat husk hydrolysates. Particularly, BK01 revealed improved tolerance against acetic acid of the hydrolysates, a major fermentation inhibitor of lignocellulosic biomass. In short, ALE with a high concentration of lactic acid improved lactic acid production as well as acetic acid tolerance of BK01, suggesting a potential for economically viable cellulosic lactic acid production.

scholarly journals Lachancea thermotolerans, the Non-Saccharomyces Yeast that Reduces the Volatile Acidity of Wines

Fermentation ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 56 ◽  
Author(s):  
Alice Vilela
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
High Concentration ◽  
Volatile Acidity ◽  
Saccharomyces Yeast ◽  
Lachancea Thermotolerans

To improve the quality of fermented drinks, or more specifically, wine, some strains of yeast have been isolated, tested and studied, such as Saccharomyces and non-Saccharomyces. Some non-conventional yeasts present good fermentative capacities and are able to ferment in quite undesirable conditions, such as the case of must, or wines that have a high concentration of acetic acid. One of those yeasts is Lachancea thermotolerants (L. thermotolerans), which has been studied for its use in wine due to its ability to decrease pH through L-lactic acid production, giving the wines a pleasant acidity. This review focuses on the recent discovery of an interesting feature of L. thermotolerans—namely, its ability to decrease wines’ volatile acidity.

scholarly journals Physiological characterization of thermotolerant yeast for cellulosic ethanol production

2014 ◽  
Vol 98 (8) ◽  
pp. 3829-3840 ◽  
Author(s):  
Daniela A. Costa ◽  
Carlos J. A. de Souza ◽  
Patrícia S. Costa ◽  
Marina Q. R. B. Rodrigues ◽  
Ancély F. dos Santos ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Thermotolerant Yeast ◽  
Physiological Characterization
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