Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil

Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).

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Socio demographic characterization of users of Child and Adolescent Psychosocial Care Centers in the state of São Paulo

Estudos de Psicologia (Campinas) ◽

10.1590/s0103-166x2013000300008 ◽

2013 ◽

Vol 30 (3) ◽

pp. 383-391 ◽

Cited By ~ 1

Author(s):

Ana Cecília Andrade de Moraes Weintraub ◽

Michelle de Sousa Vasconcellos ◽

Isabella Teixeira Bastos ◽

Felipe Lessa da Fonseca ◽

Alberto Olavo Advíncula Reis

Keyword(s):

Mental Disorders ◽

High Frequency ◽

Nuclear Family ◽

Psychosocial Care ◽

Sao Paulo ◽

The State ◽

São Paulo ◽

Severe Mental Disorders ◽

Regular School

The "Centros de Atenção Psicossocial Infanto-Juvenil" are dispositives of treatment for children and adolescents suffering from severe mental disorders. This article describes sociodemografic characteristics of users of those centers between September 2008 and February 2009 in the state of São Paulo, Brazil. Data from active files randomly selected was collected in 19 Centers in the state of São Paulo. The characteristics analyzed were divided in four groups of variables: identity; living conditions; family structure; schooling and occupation. The results indicated a higher concentration of users between 10 to 14 years-old; prevalence of males; tendency to nuclear and reduced family arrangements (45%); care primarily given by the mother (57%) and high frequency of users attending the regular school (86%). It was possible to identify a well-defined socio demographic profile of users, with differences regarding gender, attending the regular school and/or other institutions, as well as sharing a nuclear family arrangement.

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GENOTYPE CHARACTERIZATION OF Leishmania (Viannia) braziliensis ISOLATED FROM HUMAN AND CANINE BIOPSIES WITH AMERICAN CUTANEOUS LEISHMANIASIS

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652015000300013 ◽

2015 ◽

Vol 57 (3) ◽

pp. 257-262

Author(s):

Lasaro Teixeira FERREIRA ◽

Aparecida Helena de Souza GOMES ◽

Vera Lucia PEREIRA-CHIOCCOLA

Keyword(s):

Genetic Diversity ◽

Natural Populations ◽

Sao Paulo ◽

The State ◽

Internal Transcribed Spacers ◽

São Paulo ◽

Three Samples ◽

Two Samples ◽

American Tegumentary Leishmaniasis

Introduction: American tegumentary leishmaniasis (ATL) can be caused by Leishmania (Viannia) braziliensis complex. The evolution of ATL initially results in lesions and can develop into disseminated or diffuse forms. The genetic diversity of L. (V.) braziliensis in some endemic areas of Brazil has been poorly studied, such as in the state of São Paulo. This study analyzed the genetic diversity of L. (V.) braziliensis isolates collected from patients and dogs with LTA from the state of São Paulo. Methods: Leishmaniasis diagnosis was determined by PCR. The 132 biopsies were collected in different regions of Sao Paulo State, Brazil (36 municipalities). The genetic characterization of L. (V.) braziliensis isolates was tested by RFLP-PCR using DNA extracted from biopsies. The primer set amplified a specific region of Leishmania internal transcribed spacers of the ribosomal DNA locus. Results: Of the 132 samples, 52 (40%) were completely genotyped by RFLP-PCR (44 from human patients and eight from dogs). The results showed nine distinct patterns. The majority of the genotyped samples were from Sorocaba (30), and the others were distributed among 14 other municipalities. The first pattern was more frequent (29 samples), followed by pattern 2 (nine samples) and pattern 3 (three samples). Patterns 4, 6, 7, 8 and 9 were composed of two samples each and pattern 5 of one sample. Conclusion: These results suggest that polymorphic strains of L. (V.) braziliensis circulate in the state of São Paulo. These data agree with studies from other regions of Brazil, showing great variability among the natural populations of endemic foci.

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