Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cells

Abstract Odontoblasts are cells that contribute to the formation of the dental pulp complex. The differentiation of dental tissue-derived mesenchymal stem cells into odontoblasts comprises many factors and signaling pathways. Noncoding RNAs (ncRNAs), comprising a substantial part of poly-A tail mature RNAs, are considered “transcriptional noise.” Emerging evidence has shown that ncRNAs have key functions in the differentiation of mesenchymal stem cells. In this review, we discussed two major types of ncRNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), in terms of their role in the odontogenic differentiation of dental tissue-derived stem cells. Recent findings have demonstrated important functions for miRNAs and lncRNAs in odontogenic differentiation. It is expected that ncRNAs will become promising therapeutic targets for dentin regeneration based on stem cells.

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Bi-Directional Exchange of Membrane Components Occurs during Co-Culture of Mesenchymal Stem Cells and Nucleus Pulposus Cells

PLoS ONE ◽

10.1371/journal.pone.0033739 ◽

2012 ◽

Vol 7 (3) ◽

pp. e33739 ◽

Cited By ~ 51

Author(s):

Sandra Strassburg ◽

Nigel W. Hodson ◽

Patrick I. Hill ◽

Stephen M. Richardson ◽

Judith A. Hoyland

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nucleus Pulposus ◽

Nucleus Pulposus Cells ◽

Membrane Components

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Long Noncoding RNAs: New Players in the Osteogenic Differentiation of Bone Marrow- and Adipose-Derived Mesenchymal Stem Cells

Stem Cell Reviews and Reports ◽

10.1007/s12015-018-9801-5 ◽

2018 ◽

Vol 14 (3) ◽

pp. 297-308 ◽

Cited By ~ 24

Author(s):

Qiaolin Yang ◽

Lingfei Jia ◽

Xiaobei Li ◽

Runzhi Guo ◽

Yiping Huang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Noncoding Rnas ◽

Long Noncoding Rnas

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Transplantation of mesenchymal stem cells and nucleus pulposus cells in a degenerative disc model in rabbits: a comparison of 2 cell types as potential candidates for disc regeneration

Journal of Neurosurgery Spine ◽

10.3171/2010.11.spine10285 ◽

2011 ◽

Vol 14 (3) ◽

pp. 322-329 ◽

Cited By ~ 59

Author(s):

Ganjun Feng ◽

Xianfeng Zhao ◽

Hao Liu ◽

Huina Zhang ◽

Xiangjun Chen ◽

...

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nucleus Pulposus ◽

Disc Degeneration ◽

Signal Intensity ◽

Disc Height ◽

Nucleus Pulposus Cells ◽

Disc Regeneration ◽

Disc Model

Object The aim of this study was to compare transplanted mesenchymal stem cells (MSCs) with nucleus pulposus cells (NPCs) in a degenerative disc model in rabbits to determine the better candidate for disc cell therapy. Methods Mesenchymal stem cells and NPCs were transplanted in a rabbit model of disc degeneration. Changes in disc height, according to plain radiography, T2-weighted signal intensity on MR imaging, histology, sulfated glycosaminoglycan (sGAG)/DNA, and associated gene expression levels, were evaluated among healthy controls without surgery, sham-operated animals in which only disc degeneration was induced, MSC-transplanted animals, and NPC-transplanted animals for a 16-week period. Results Sixteen weeks after cell transplantation, in the MSC- and NPC-transplanted groups, the decline in the disc height index was reduced and T2-weighted signal intensity increased compared with the sham-operated group. Safranin O staining showed a high GAG content, which was also supported by sGAG/DNA assessment. Disc regeneration was also confirmed at the gene expression level using real-time polymerase chain reaction. However, no significant differences in expression were found between the NPC- and MSC-transplanted groups. Conclusions Study data showed that MSC transplantation is effective for the treatment of disc degeneration and seems to be an ideal substitute for NPCs.

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Effect of Lentivirus-mediated GDF5 Transfection on Differentiation of Rabbit Nucleus Pulposus Mesenchymal Stem Cells

10.21203/rs.3.rs-40034/v1 ◽

2020 ◽

Author(s):

kun zhu ◽

Rui Zhao ◽

Yuchen Ye ◽

Gang Xu ◽

Changchun Zhang

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nucleus Pulposus ◽

Lentiviral Vector ◽

Intervertebral Discs ◽

Degenerative Disease ◽

The Other ◽

Control Group ◽

Nucleus Pulposus Cells ◽

Qrt Pcr

Abstract Background: Disc degenerative disease is a common senile degenerative disease, which seriously affects the quality of life of patients.The purpose of this study is to observe the biological and cytological characteristics of rabbit nucleus pulposus mesenchymal stem cells (NPMSCs), and to determine the effect of growth differentiation factor 5(GDF5) on the differentiation of rabbit NPMSCs by lentivirus transfection.Methods: In vitro culture model of rabbit NPMSCs was established and NPMSCs cells were identified by flow cytometry (FCM)and quantitative real-time PCR(qRT-PCR). Then NPMSCs were divided into three groups: lentiviral vector carrying GDF5 was used to transfect NPMSCs, to determine the transfection rate, which was recorded as transfection group, and the NPMSCs transfected with ordinary lentiviral vector was recorded as control group, NPMSCs without processing was recorded as normal group. FCM, qRT-PCR and Western Blot(WB) were used to detected the change of NPMSCs.Results: The transfected NPMSCs by GDF5 became longer and narrower, and the cell density decreased,and the positive rate of GDF5 in the transfected group was significantly higher than that in the other two groups (P<0.05). The mRNA expression of KRT8, KRT18, KRT19 in the transfected group was significantly higher than the other two groups(P<0.05),the result of WB were the same to qRT-PCR. Conclusions: GDF5 can induce the differentiation of NPMSCs and repair degenerative intervertebral discs. Lentiviral vector carrying GDF5 can be integrated into the chromosome genome of NPMSCs and promote differentiation of NPMSCs into nucleus pulposus cells(NPCs).

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Dynamic compression and co-culture with nucleus pulposus cells promotes proliferation and differentiation of adipose-derived mesenchymal stem cells

Journal of Biomechanics ◽

10.1016/j.jbiomech.2014.01.023 ◽

2014 ◽

Vol 47 (5) ◽

pp. 966-972 ◽

Cited By ~ 17

Author(s):

Jun Dai ◽

Huan Wang ◽

Guo Liu ◽

Zhanjiang Xu ◽

Feng Li ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nucleus Pulposus ◽

Dynamic Compression ◽

Nucleus Pulposus Cells ◽

Proliferation And Differentiation

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MicroRNAs and long noncoding RNAs: new regulators in cell fate determination of mesenchymal stem cells

RSC Advances ◽

10.1039/c9ra06563f ◽

2019 ◽

Vol 9 (64) ◽

pp. 37300-37311 ◽

Cited By ~ 1

Author(s):

Zixiang Wu ◽

Shujing Liang ◽

Wenyu Kuai ◽

Lifang Hu ◽

Airong Qian

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cell Fate ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Cell Fate Determination ◽

Fate Determination ◽

Recent Advances

The recent advances of miRNAs and lncRNAs in determining the cell fate of MSCs.

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Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells

Stem Cells International ◽

10.1155/2016/6205485 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

Shihua Wang ◽

Xiaoxia Li ◽

Robert Chunhua Zhao

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Molecular Mechanisms ◽

Expression Patterns ◽

Length Distribution ◽

Noncoding Rnas ◽

Long Noncoding Rnas ◽

Differentially Expressed ◽

Therapeutic Effects ◽

Wide Range

Mesenchymal stem cells (MSCs) possess great immunomodulatory capacity which lays the foundation for their therapeutic effects in a variety of diseases. Recently, toll-like receptors (TLR) have been shown to modulate MSC functions; however, the underlying molecular mechanisms are poorly understood. Emerging evidence suggests that long noncoding RNAs (lncRNAs) are an important class of regulators involved in a wide range of biological processes. To explore the potential involvement of lncRNAs in TLR stimulated MSCs, we performed a comprehensive lncRNA and mRNA profiling through microarray. 10.2% of lncRNAs (1733 out of 16967) and 15.1% of mRNA transcripts (1760 out of 11632) were significantly differentially expressed (absolute fold-change≥5 ,Pvalue≤0.05) in TLR3 stimulated MSCs. Furthermore, we characterized the differentially expressed lncRNAs through their classes and length distribution and correlated them with differentially expressed mRNA. Here, we are the first to determine genome-wide lncRNAs expression patterns in TLR3 stimulated MSCs by microarray and this work could provide a comprehensive framework of the transcriptome landscapes of TLR3 stimulated MSCs.

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