scholarly journals Does intra-articular injection of adipose-derived stem cells improve cartilage mass? A case report using three-dimensional image analysis software in knee osteoarthritis

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Ayano Kuwasawa ◽  
Kotaro Nihei
Keyword(s):  
Stem Cells ◽  
Image Analysis ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Analysis Software ◽  
Mesenchymal Stem Cell Therapy ◽  
Dimensional Image ◽  
Image Analysis Software ◽  
Before And After

Abstract Background Mesenchymal stem cells are currently a research focus because of the possibility of cartilage regeneration through several mechanisms, including mesenchymal stem cell sheets. However, there are no published reports visualizing cartilage in three dimensions. Here, we report a case of improved cartilage volume. We purified and cultured adipose-derived mesenchymal stem cells and then performed adipose-derived mesenchymal stem cell therapy by directly injecting these cells into the articular cartilage. Cartilage was quantitatively evaluated before and after injection using three-dimensional image analysis software based on the magnetic resonance imaging. Case presentation The patient, a 55-year-old Japanese woman, experienced pain in both knees and was diagnosed with osteoarthritis of the knee. We performed adipose-derived mesenchymal stem cell therapy in both knees at our hospital and quantitatively evaluated cartilage before and after the treatment using the three-dimensional image analysis software “SYNAPSE VINCENT”. Conclusions Preoperatively, the cartilage defect area was 33.59 mm2 in the femur and 122.31 mm2 in the tibia; however, 12 months postoperatively, it improved to 13.59 mm2 and 51.43 mm2, respectively. Furthermore, the preoperative femur and tibia volumes were 9.58 mL and 3.82 mL, respectively; however, 12 months postoperatively, these values improved to 10.00 mL and 4.17 mL, respectively. For the quantitative analysis of cartilage, SYNAPSE VINCENT visualizes the state of cartilage in a high-definition three-dimensional image, which is excellent for understanding the state of the disease and explaining it to the patient. Although SYNAPSE VINCENT can only analyze the thickness of cartilage, and the reproducibility of the error is debatable, SYNAPSE VINCENT would be useful as a clinical tool for regenerative medicine. We have shown in this case report the promising effects of adipose-derived stem cell intraarticular injections in treating osteoarthritis and the use of new diagnostic instruments.

scholarly journals Does intra-articular injection of adipose-derived stem cells improve cartilage mass? A case report on a 3D MRI quantitative evaluation of cartilage in knee osteoarthritis using SYNAPSE VINCENT: a case report

2020 ◽  
Author(s):  
Ayano Kuwasawa ◽  
Kotaro Nihei
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Image Analysis ◽  
Case Report ◽  
The State ◽  
3D Image ◽  
Analysis Software ◽  
3D Image Analysis ◽  
Image Analysis Software ◽  
Before And After

Abstract Background: Mesenchymal stem cells (MSC) are currently in focus because of the possibility of cartilage regeneration through several ways, including MSC sheets. However, there is no published report that visualizes cartilage in three dimensions. Here, we report a case of improved cartilage volume. We purified and cultured adipose-derived mesenchymal stem cells (ASC) and then performed ASC therapy by directly injecting these cells into the articular cartilage. Cartilage was quantitatively evaluated before and after injection using a three-dimensional (3D) image analysis software based on the MRI imagery.Case presentation: The patient, a 55-year-old woman, experienced pain in both knees and was diagnosed with osteoarthritis of the knee. We performed ASC therapy in both knees at our hospital and quantitatively evaluated cartilage before and after the treatment using the 3D image analysis software “SYNAPSE VINCENT”.Conclusions: For the quantitative analysis of cartilage, SYNAPSE VINCENT visualizes the state of cartilage in a high-definition 3D image, which is excellent for understanding the state of the disease and explaining it to the patient. Though there is room for debate about the reproducibility of errors, etc., SYNAPSE VINCENT would be useful as a clinical tool for regenerative medicine.

scholarly journals Does intra-articular injection of adipose-derived stem cells improve cartilage mass? A case report on a 3D MRI quantitative evaluation of cartilage in knee osteoarthritis using SYNAPSE VINCENT: a case report

2020 ◽  
Author(s):  
Ayano Kuwasawa ◽  
Kotaro Nihei
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Image Analysis ◽  
Case Report ◽  
The State ◽  
3D Image ◽  
Analysis Software ◽  
3D Image Analysis ◽  
Image Analysis Software ◽  
Before And After

Abstract Background: Mesenchymal stem cells (MSC) are currently in focus because of the possibility of cartilage regeneration through several ways, including MSC sheets. However, there is no published report that visualizes cartilage in three dimensions. Here, we report a case of improved cartilage volume. We purified and cultured adipose-derived mesenchymal stem cells (ASC) and then performed ASC therapy by directly injecting these cells into the articular cartilage. Cartilage was quantitatively evaluated before and after injection using a three-dimensional (3D) image analysis software based on the MRI imagery.Case presentation: The patient, a 55-year-old woman, experienced pain in both knees and was diagnosed with osteoarthritis of the knee. We performed ASC therapy in both knees at our hospital and quantitatively evaluated cartilage before and after the treatment using the 3D image analysis software “SYNAPSE VINCENT”.Conclusions: For the quantitative analysis of cartilage, SYNAPSE VINCENT visualizes the state of cartilage in a high-definition 3D image, which is excellent for understanding the state of the disease and explaining it to the patient. Though there is room for debate about the reproducibility of errors, etc., SYNAPSE VINCENT would be useful as a clinical tool for regenerative medicine.

scholarly journals Anatomical Characteristics of Garcinia lucida (Vesque) and Scorodophloeus zenkeri (Harms) Wood and Debarking Response in the South Region Cameroon

2017 ◽  
Vol 6 (4) ◽  
pp. 132
Author(s):  
Marie Caroline Momo Solefack ◽  
Hans Beeckman ◽  
Lucie Felicite Temgoua ◽  
Ghislain Kenguem Kinjouo
Keyword(s):  
Image Analysis ◽  
Cross Section ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Analysis Software ◽  
Anatomical Changes ◽  
Image Analysis Software ◽  
Conductive Zone ◽  
Before And After

The aim of this work was to investigate the possible anatomical changes of Garcinia lucida and Scorodophloeus zenkeri after the removal of their bark. Debarking was done on individuals of each species at 1.30 m from the soil. The wound was rectangular in shape with 30 cm side. There was a follow-up every three months for nine months during which the survival and rate of regeneration of the bark were recorded. A block of cube was cut from the regenerated and intact wood of species for microtomy and microscopy activities. On the cross-section of each wood, vessel features like density and diameter were measured before and after wounding. Semi-automatic measurements were made using the SpectrumSee digital image analysis software. In the wood of the two species, it appeared that the density of the vessels before debarking was significantly comparable to the density after debarking, while the diameter of vessels in the regenerated wood was smaller. The cambial area increased slightly in the rainy season for all species. After nine months all the species started the restoration of their conductive zone. G. lucida heals its wound more rapidly than S. zenkeri.

scholarly journals Automated three-dimensional measurements of version, inclination, and subluxation

2019 ◽  
Vol 12 (1) ◽  
pp. 31-37
Author(s):  
Dave R Shukla ◽  
Richard J McLaughlin ◽  
Julia Lee ◽  
Ngoc Tram V Nguyen ◽  
Joaquin Sanchez-Sotelo
Keyword(s):  
Computed Tomography ◽  
Image Analysis ◽  
Three Dimensional ◽  
Automated Image Analysis ◽  
Two Dimensional ◽  
Analysis Software ◽  
Glenoid Version ◽  
Image Analysis Software ◽  
Planning Software ◽  
Dimensional Measurements

Background Preoperative planning software has been developed to measure glenoid version, glenoid inclination, and humeral head subluxation on computed tomography (CT) for shoulder arthroplasty. However, most studies analyzing the effect of glenoid positioning on outcome were done prior to the introduction of planning software. Thus, measurements obtained from the software can only be extrapolated to predict failure provided they are similar to classic measurements. The purpose of this study was to compare measurements obtained using classic manual measuring techniques and measurements generated from automated image analysis software. Methods Ninety-five two-dimensional computed tomography scans of shoulders with primary glenohumeral osteoarthritis were measured for version according to Friedman method, inclination according to Maurer method, and subluxation according to Walch method. DICOM files were loaded into an image analysis software (Blueprint, Wright Medical) and the output was compared with values obtained manually using a paired sample t-test. Results Average manual measurements included 13.8° version, 13.2° inclination, and 56.2% subluxation. Average image analysis software values included 17.4° version (3.5° difference, p < 0.0001), 9.2° inclination (3.9° difference, p < 0.001), and 74.2% for subluxation (18% difference, p < 0.0001). Conclusions Glenoid version and inclination values from the software and manual measurement on two-dimensional computed tomography were relatively similar, within approximately 4°. However, subluxation measurements differed by approximately 20%.

Should Mesenchymal Stem Cell Therapy be used in the treatment of COVID-19?

2020 ◽  
Vol 54 ◽  
Author(s):  
Namnama P. Villarta-De Dios ◽  
Evelyn S. Osio-Salido
Keyword(s):  
Stem Cells ◽  
Randomized Controlled Trial ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Standard Treatment ◽  
Controlled Trial ◽  
Mesenchymal Stem Cell Therapy ◽  
Randomized Controlled

KEY FINDINGSThere is some short-term benefit from the use of mesenchymal stem cell therapy for severe COVID-19 in a lowquality, small randomized controlled trial (RCT). Further studies, ideally with good quality RCTs, are needed toconfirm benefit and safety.• Mesenchymal stem cells (MSCs) are non-hematopoietic adult stem cells that are able to self-renew anddifferentiate into various cells of any cell lineage. MSCs have the ability to migrate (homing) to damaged tissuefor repair and regeneration, as well as signal other cells to help in tissue repair. It can effect cellular differentiation,consequently shifting the immune system from Th1 to Th2 responses.1,2• A recent review showed improved disease-associated parameters in experimental acute respiratory distresssyndrome (ARDS).3• We found three studies (one case report, one prospective cohort, and one small randomized controlled trial)reporting the effects of MSC on COVID-19. Based on low-quality evidence, it appeared that human umbilicalcord blood-derived MSC, given after failure to improve with standard treatment, had beneficial effects in termsof earlier onset of clinical improvement among individuals with severe or critical COVID-19. However, 28-dayclinical improvement and mortality were not significantly different compared to standard treatment. There wasno reported adverse reaction.• There are 52 registered and ongoing clinical trials to investigate the efficacy and safety of mesenchymal stemcells as treatment for COVID-19.• Mesenchymal stem cell therapy is not included in any of the existing guidelines for the treatment of COVID-19.

Mesenchymal Stem Cell Therapy for Nonmusculoskeletal Diseases: Emerging Applications

2009 ◽  
Vol 18 (9) ◽  
pp. 1013-1028 ◽  
Author(s):  
Tom K. Kuo ◽  
Jennifer H. Ho ◽  
Oscar K. Lee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Musculoskeletal Diseases ◽  
Cartilage Regeneration ◽  
Building Blocks ◽  
Differentiation Potential ◽  
Mesenchymal Stem Cell Therapy

Mesenchymal stem cells are stem/progenitor cells originated from the mesoderm and can different into multiple cell types of the musculoskeletal system. The vast differentiation potential and the relative ease for culture expansion have established mesenchymal stem cells as the building blocks in cell therapy and tissue engineering applications for a variety of musculoskeletal diseases, including repair of fractures and bone defects, cartilage regeneration, treatment of osteonecrosis of the femoral head, and correction of genetic diseases such as osteogenesis imperfect. However, research in the past decade has revealed differentiation potentials of mesenchymal stem cells beyond lineages of the mesoderm, suggesting broader applications than originally perceived. In this article, we review the recent developments in mesenchymal stem cell research with respect to their emerging properties and applications in nonmusculoskeletal diseases.

scholarly journals ID3026 Current use and clinical studies of stem cells therapy for osteoarthritis in Viet Nam

2017 ◽  
Vol 4 (S) ◽  
pp. 36
Author(s):  
Tung Dang-Xuan Tran
Keyword(s):  
Rheumatoid Arthritis ◽  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Viet Nam ◽  
Medical Problems ◽  
Mesenchymal Stem Cell Therapy ◽  
Advanced Therapies ◽  
Stem Cells Therapy

Mesenchymal Stem Cell Therapy (MSC) is one of the newest and most advanced therapies in Regenerative and Integrative Medicine for a variety of medical problems that are not easily treated with traditional conventional allopathic medical approaches. There is an abundance of clinical experience by numerous physicians, patients and researchers proving consistently that mesenchymal stem cell-MSC therapy can be extremely helpful in Osteoarthritis and Rheumatoid Arthritis. In Viet Nam we have several researches in stem cells clinical application in OA

scholarly journals Will Mesenchymal Stem Cell Therapy Be Effective In COVID-19?

2021 ◽  
Vol 11 (6) ◽  
pp. 281-285
Author(s):  
Srinivas Kalai ◽  
M Senthil ◽  
R Sambath Kumar ◽  
R Kameshwaran
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Immune System ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Umbilical Cord ◽  
Stem Cell Therapy ◽  
Human Umbilical Cord ◽  
Cytokine Storm ◽  
Mesenchymal Stem Cell Therapy

Background: The World Health Organization (WHO) reports that the outbreak of the deadly virus had been noted almost in all the countries worldwide. Newly no standard therapies are available to combat the situation and this remains the major challenge for healthcare professionals to provide effective treatment against the life-threatening condition. A potential regenerative medicine method using the infusion of stem cells for the treatment of lung disorders has been reported. This review attempted to explore the immunomodulatory characteristics of Mesenchymal Stem Cells (MSCs) and how these properties make them beneficial for the treatment of Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) patients. Objectives: To study the effect of Mesenchymal Stem Cell therapy in treating COVID-19. Methodology: A literature search was conducted to identify recent research relating to the review's goal of analyzing the relevance of stem cells in battling SARS-CoV-2. Results: The MSCs settle in the lungs intravenously to enhance the pulmonary microenvironment, minimize immune system over-activation, and encourage regeneration of damaged lung tissues. Its therapeutic properties like immune response inhibition play a major role in combating viruses. The avoidance of cytokine storm is the most important stage in COVID-19 therapy. Their potent immunomodulatory properties have positive effects in avoiding or attenuating the cytokine storm and assisting in the regeneration of injured lung tissues/other organs. Conclusion: Intravenous human Umbilical Cord-Mesenchymal Stem Cell therapy (hUC-MSC) transplantation is a safe and effective technique that may be used as a restoration and prioritized therapeutic option for treating severe COVID-19. Keywords: Covid-19, human Umbilical Cord-Mesenchymal Stem Cell therapy (huc-msc), Immune system.

Application of Bone Marrow–Derived Mesenchymal Stem Cells for Muscle Healing After Contusion Injury in Mice

2020 ◽  
Vol 48 (5) ◽  
pp. 1226-1235 ◽  
Author(s):  
Chih-Hao Chiu ◽  
Tsan-Hsuan Chang ◽  
Shih-Sheng Chang ◽  
Gwo-Jyh Chang ◽  
Alvin Chao-Yu Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Mesenchymal Stem Cell Therapy ◽  
Contusion Injury ◽  
Fast Twitch

Background: Skeletal muscle injuries are very common in sports medicine. Conventional therapies have limited clinical efficacy. New treatment methods should be developed to allow athletes to return to play with better function. Purpose: To evaluate the in vitro differentiation potential of bone marrow–derived mesenchymal stem cells and the in vivo histologic and physiologic effects of mesenchymal stem cell therapy on muscle healing after contusion injury. Study Design: Controlled laboratory study. Methods: Bone marrow cells were flushed from both femurs of 5-week-old C57BL/6 mice to establish immortalized mesenchymal stem cell lines. A total of 36 mice aged 8 to 10 weeks were used to develop a muscle contusion model and were divided into 6 groups (6 mice/group) on the basis of the different dosages of IM2 cells to be injected (0, 1.25 × 105, and 2.5 × 105 cells with/without F-127 in 100 μL of phosphate-buffered saline). Histological analysis of muscle regeneration was performed, and the fast-twitch and tetanus strength of the muscle contractions was measured 28 days after muscle contusion injury, after injections of different doses of mesenchymal stem cells with or without the F-127 scaffold beginning 14 days after contusion injury. Results: The mesenchymal stem cell–treated muscles exhibited numerous regenerating myofibers. All the groups treated with mesenchymal stem cells (1.25 × 105 cells, 2.5 × 105 cells, 1.25 × 105 cells plus F-127, and 2.5 × 105 cells plus F-127) exhibited a significantly higher number of regenerating myofibers (mean ± SD: 111.6 ± 14.77, 133.4 ± 21.44, 221.89 ± 32.65, and 241.5 ± 25.95, respectively) as compared with the control group and the control with F-127 (69 ± 18.79 and 63.2 ± 18.98). The physiologic evaluation of fast-twitch and tetanus strength did not reveal differences between the age-matched uninjured group and the groups treated with various doses of mesenchymal stem cells 28 days after contusion. Significant differences were found between the control group and the groups treated with various doses of mesenchymal stem cells after muscle contusion. Conclusion: Mesenchymal stem cell therapy increased the number of regenerating myofibers and improved fast-twitch and tetanus muscle strength in a mouse model of muscle contusion. However, the rapid decay of transplanted mesenchymal stem cells suggests a paracrine effect of this action. Treatment with mesenchymal stem cells at various doses combined with the F-127 scaffold is a potential therapy for a muscle contusion. Clinical Relevance: Mesenchymal stem cell therapy has an effect on sports medicine because of its effects on myofiber regeneration and muscle strength after contusion injury.

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