scholarly journals A cationic quantum dot-based ratiometric fluorescent probe to visually detect berberine hydrochloride in human blood serums

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mingyang Liu ◽  
Xuejun Du ◽  
Ke Xu ◽  
Binwei Yan ◽  
Zaibi Fan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Human Blood ◽  
Cetylpyridinium Chloride ◽  
High Sensitivity ◽  
Water Soluble ◽  
Detection Methods ◽  
Detection Range ◽  
Berberine Hydrochloride ◽  
Relative Standard ◽  
Ratiometric Fluorescent Probe

AbstractBerberine hydrochloride (BH) is an isoquinoline alkaloid normally used as drug to treat diseases. Compared with the traditional detection methods, the carbon quantum dots (CQDs) have better selectivity, high sensitivity, easy operation, and is inexpensive which could be widely utilized as fluorescent nanoprobes to detect various compounds quantificationally. And ratiometric fluorescent sensors conspicuously increase sensitivity and precision detection and improve quantification. In this work, we use water-soluble and fluorescent cationic carbon dots cetylpyridinium chloride monohydrate (CPC)-CQDs to connect with pinacyanol chloride (PC) and sodium tetraphenylborate (ST) as the ratiometric fluorescent probe to detect BH. The ratiometric fluorescent probe has high sensitivity towards alkaloids and metal ions, photochemical stability (60 min), and pH stability (from 6.0 to 8.0), with the detection range from 0 to 200 μM, and limit was as low as 57.35 nM. The accuracy of the method was verified by spiked recovery experiment in different human blood serums which were drawn from healthy adult volunteers to explore the practicability. The recoveries were in the range 94.34 to 105.48% with relative standard deviations (RSD) of 0.80 to 2.92%. In addition, we could observe that the fluorescence was gradually darkened, and the color turned yellow to realize the visual detection. It is expected that this work would open up a new strategy for detecting BH in the environment and human blood serums.

A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

2021 ◽  
Vol 368 (6) ◽  
Author(s):  
Liwen Zhang ◽  
Qingyu Lv ◽  
Yuling Zheng ◽  
Xuan Chen ◽  
Decong Kong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Method ◽  
Detection Methods ◽  
Cereal Crops ◽  
Detection Range ◽  
Highly Sensitive ◽  
Food And Feed ◽  
Good Repeatability ◽  
Feed Samples ◽  
Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

Near-infrared mito-specific fluorescent probe for ratiometric detection and imaging of alkaline phosphatase activity with high sensitivity

2019 ◽  
Vol 7 (3) ◽  
pp. 443-450 ◽  
Author(s):  
Qian Zhang ◽  
Shasha Li ◽  
Caixia Fu ◽  
Yuzhe Xiao ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Fluorescent Probe ◽  
Alkaline Phosphatase Activity ◽  
Near Infrared ◽  
High Sensitivity ◽  
Cyanine Dye ◽  
Intracellular Imaging ◽  
Ratiometric Detection ◽  
Ratiometric Fluorescent Probe

A NIR ratiometric fluorescent probe based on cyanine dye was developed for detecting and intracellular imaging of ALP activity with high sensitivity.

A Novel Water-soluble Ratiometric Fluorescent Probe Based on FRET for Sensing Lysosomal pH

2016 ◽  
Vol 26 (6) ◽  
pp. 2079-2086 ◽  
Author(s):  
Guang-Jie Song ◽  
Su-Yun Bai ◽  
Jing Luo ◽  
Xiao-Qun Cao ◽  
Bao-Xiang Zhao
Keyword(s):  
Fluorescent Probe ◽  
Water Soluble ◽  
Lysosomal Ph ◽  
Ratiometric Fluorescent Probe

scholarly journals Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-Kyung Oh ◽  
Kihyeun Kim ◽  
Jinhee Park ◽  
Hyungjun Jang ◽  
Min-Gon Kim
Keyword(s):  
Polyvinyl Alcohol ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Ease Of Use ◽  
Water Soluble ◽  
Stress Disorders ◽  
Lateral Flow Immunoassay ◽  
Detection Range ◽  
Bodily Fluids

AbstractPaper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL−1) over a broad detection range (0.01–1000 ng∙mL−1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human real saliva samples using this sensor and a conventional ELISA (R2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.

A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5472-5478 ◽  
Author(s):  
Peng Zhang ◽  
Shasha Li ◽  
Caixia Fu ◽  
Qian Zhang ◽  
Yuzhe Xiao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
High Sensitivity ◽  
Tyrosinase Activity ◽  
Cyanine Dye ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe for TYR activity with high sensitivity was developed based on the H-aggregation of a cyanine dye.

A novel near-infrared ratiometric fluorescent probe for SO2 detection with a large emission shift

2020 ◽  
Vol 44 (11) ◽  
pp. 4554-4557 ◽  
Author(s):  
Shaohui Han ◽  
Xiuxiu Yue ◽  
Jingpei Wang ◽  
Yun Zhang ◽  
Benhua Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
High Sensitivity ◽  
Sensitivity And Selectivity ◽  
Ratiometric Fluorescent Probe

A novel near-infrared ratiometric fluorescent probe MQC (λem = 715 nm) for SO2 detection was developed with a large emission shift (245 nm) and high sensitivity and selectivity.

A dual-ratiometric fluorescent probe for individual and continuous detection of H2S and HClO in living cells

2020 ◽  
Vol 56 (19) ◽  
pp. 2849-2852 ◽  
Author(s):  
Xiuxiu Yue ◽  
Jingpei Wang ◽  
Jinliang Han ◽  
Benhua Wang ◽  
Xiangzhi Song
Keyword(s):  
Fluorescent Probe ◽  
High Sensitivity ◽  
Living Cells ◽  
The Individual ◽  
Ratiometric Fluorescent Probe

A dual-ratiometric fluorescent probe, Han-HClO-H2S, was developed for the individual and continuous detection of H2S and HClO with high sensitivity and good selectivity, and had been applied to detect intracellular H2S and/or HClO in living cells.

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