scholarly journals Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-Kyung Oh ◽  
Kihyeun Kim ◽  
Jinhee Park ◽  
Hyungjun Jang ◽  
Min-Gon Kim
Keyword(s):  
Polyvinyl Alcohol ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Ease Of Use ◽  
Water Soluble ◽  
Stress Disorders ◽  
Lateral Flow Immunoassay ◽  
Detection Range ◽  
Bodily Fluids

AbstractPaper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL−1) over a broad detection range (0.01–1000 ng∙mL−1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human real saliva samples using this sensor and a conventional ELISA (R2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.

scholarly journals Advanced Trap Lateral Flow Immunoassay Sensor for the Detection of Cortisol in Human Bodily Fluids

2021 ◽  
Author(s):  
Hyun-Kyung Oh ◽  
Kihyeun Kim ◽  
Hyungjun Jang ◽  
Jinhee Park ◽  
Min-Gon Kim
Keyword(s):  
Polyvinyl Alcohol ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Ease Of Use ◽  
Water Soluble ◽  
Stress Disorders ◽  
Lateral Flow Immunoassay ◽  
Detection Range ◽  
Bodily Fluids

Abstract Paper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL-1) over a broad detection range (0.01–1000 ng∙mL-1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human saliva samples using this sensor and a conventional ELISA (R2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.

scholarly journals Lateral Flow Immunoassay with Quantum-Dot-Embedded Silica Nanoparticles for Prostate-Specific Antigen Detection

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Sungje Bock ◽  
Hyung-Mo Kim ◽  
Jaehi Kim ◽  
Jaehyun An ◽  
Yun-Sik Choi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Silica Nanoparticles ◽  
Prostate Specific Antigen ◽  
False Negative ◽  
Specific Antigen ◽  
Calf Serum ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay

Prostate cancer can be detected early by testing the presence of prostate-specific antigen (PSA) in the blood. Lateral flow immunoassay (LFIA) has been used because it is cost effective and easy to use and also has a rapid sample-to-answer process. Quantum dots (QDs) with very bright fluorescence have been previously used to improve the detection sensitivity of LFIAs. In the current study, a highly sensitive LFIA kit was devised using QD-embedded silica nanoparticles. In the present study, only a smartphone and a computer software program, ImageJ, were used, because the developed system had high sensitivity by using very bright nanoprobes. The limit of PSA detection of the developed LFIA system was 0.138 ng/mL. The area under the curve of this system was calculated as 0.852. The system did not show any false-negative result when 47 human serum samples were analyzed; it only detected PSA and did not detect alpha-fetoprotein and newborn calf serum in the samples. Additionally, fluorescence was maintained on the strip for 10 d after the test. With its high sensitivity and convenience, the devised LFIA kit can be used for the diagnosis of prostate cancer.

scholarly journals A cationic quantum dot-based ratiometric fluorescent probe to visually detect berberine hydrochloride in human blood serums

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mingyang Liu ◽  
Xuejun Du ◽  
Ke Xu ◽  
Binwei Yan ◽  
Zaibi Fan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Human Blood ◽  
Cetylpyridinium Chloride ◽  
High Sensitivity ◽  
Water Soluble ◽  
Detection Methods ◽  
Detection Range ◽  
Berberine Hydrochloride ◽  
Relative Standard ◽  
Ratiometric Fluorescent Probe

AbstractBerberine hydrochloride (BH) is an isoquinoline alkaloid normally used as drug to treat diseases. Compared with the traditional detection methods, the carbon quantum dots (CQDs) have better selectivity, high sensitivity, easy operation, and is inexpensive which could be widely utilized as fluorescent nanoprobes to detect various compounds quantificationally. And ratiometric fluorescent sensors conspicuously increase sensitivity and precision detection and improve quantification. In this work, we use water-soluble and fluorescent cationic carbon dots cetylpyridinium chloride monohydrate (CPC)-CQDs to connect with pinacyanol chloride (PC) and sodium tetraphenylborate (ST) as the ratiometric fluorescent probe to detect BH. The ratiometric fluorescent probe has high sensitivity towards alkaloids and metal ions, photochemical stability (60 min), and pH stability (from 6.0 to 8.0), with the detection range from 0 to 200 μM, and limit was as low as 57.35 nM. The accuracy of the method was verified by spiked recovery experiment in different human blood serums which were drawn from healthy adult volunteers to explore the practicability. The recoveries were in the range 94.34 to 105.48% with relative standard deviations (RSD) of 0.80 to 2.92%. In addition, we could observe that the fluorescence was gradually darkened, and the color turned yellow to realize the visual detection. It is expected that this work would open up a new strategy for detecting BH in the environment and human blood serums.

scholarly journals Serotype Sensitivity of a Lateral Flow Immunoassay for Cryptococcal Antigen

2013 ◽  
Vol 20 (4) ◽  
pp. 634-635 ◽  
Author(s):  
Marcellene A. Gates-Hollingsworth ◽  
Thomas R. Kozel
Keyword(s):  
High Sensitivity ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Global Community ◽  
Cryptococcal Antigen

ABSTRACTTo meet the needs of a global community, an immunoassay for cryptococcal antigen (CrAg) must have high sensitivity for CrAg of all major serotypes. A new immunoassay for CrAg in lateral flow format was evaluated and found to have a high sensitivity for detection of serotypes A, B, C, and D.

Nanozyme amplification mediated on-demand multiplex lateral flow immunoassay with dual-readout and broadened detection range

2020 ◽  
Vol 169 ◽  
pp. 112610 ◽  
Author(s):  
Sijie Liu ◽  
Leina Dou ◽  
Xiaolin Yao ◽  
Wentao Zhang ◽  
Man Zhao ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Detection Range ◽  
On Demand

scholarly journals Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection

Biosensors ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 13
Author(s):  
Tao Peng ◽  
Xueshima Jiao ◽  
Zhanwei Liang ◽  
Hongwei Zhao ◽  
Yang Zhao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Nucleocapsid Protein ◽  
High Efficiency ◽  
Protein Detection ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Copper Deposition ◽  
Detection Sensitivity ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.

scholarly journals Development of a Europium Nanoparticles Lateral Flow Immunoassay for NGAL Detection in Urine and Diagnosis of Acute Kidney Injury

2021 ◽  
Author(s):  
Moli Yin ◽  
Yuanwang Nie ◽  
Hao Liu ◽  
Lei Liu ◽  
Lu Tang ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Kidney Injury ◽  
Lateral Flow ◽  
Adverse Outcomes ◽  
Detection Methods ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Detection Range ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Europium Nanoparticles

Abstract Background:AKI is related to severe adverse outcomes and mortality with Coronavirus Infection Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney injury (AKI), but current detection methods are inadequacy, so more rapid, convenient and accuracy methods are needed to detect NGAL for early diagnosis of AKI. Herein, we established a rapid, reliable and accuracy lateral flow immunoassay based on europium nanoparticles (Eu-NPS-LFIA) for the detection of NGAL in human urine specimens. Methods:A double-antibody sandwich immunofluorescent assay using europium doped nanoparticles was employed and the NGAL monoclonal antibodies conjugate as labels were generated by optimizing electric fusion parameters. Eighty-three urine samples were used to evaluate the clinical application efficiency of this method. Results:The quantitative detection range of NGAL in AKI was 1-3000 ng/mL, and the detection sensitization was 0.36 ng/mL. The CV of intra-assay and inter-assay were 2.57%-4.98% and 4.11%-7.83%, respectively. Meanwhile, the correlation coefficient between Eu-NPS-LFIA and ARCHITECT analyzer was significant (R2=0.9829, n=83, p<0.01). Conclusions:Thus, a faster and easier operation quantitative assay of NGAL for AKI has been established, which is very important and meaningful to diagnose the early AKI, suggesting that the assay can provide an early warning of final outcome of disease.

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