scholarly journals Biological pretreatment of corn stover for enhancing enzymatic hydrolysis using Bacillus sp. P3

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yanwen Wu ◽  
Haipeng Guo ◽  
Md. Shafiqur Rahman ◽  
Xuantong Chen ◽  
Jinchi Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Fermentation Broth ◽  
Removal Rate ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Biological Pretreatment ◽  
Hydrolysis Process ◽  
Hemicellulose Removal ◽  
Hydrolysis Of

AbstractThe biological pretreatment for the enzymatic hydrolysis of lignocellulosic biomasses depends exclusively on the effective pretreatment process. Herein, we report a significant enhancement of enzymatic saccharification obtained with corn stover using a bacterial strain Bacillus sp. P3. The hemicellulose removal from corn stover by the strain Bacillus sp. P3 was evaluated for enhancing subsequent enzymatic hydrolysis. Therefore, our study revealed that an alkaline-resistant xylanase as well as other enzymes produced by Bacillus sp. P3 in fermentation broth led to a substantially enhanced hemicellulose removal rate from corn stover within pH 9.36–9.68. However, after a 20-day pretreatment of corn stover by the strain P3, the glucan content was increased by 51% and the xylan content was decreased by 35%. After 72 h of saccharification using 20 U/g of commercial cellulase, the yield of reducing sugar released from 20-day pretreated corn stover was increased by 56% in comparison to the untreated corn stover. Therefore, the use of the strain P3 could be a promising approach to pretreat corn stover for enhancing the enzymatic hydrolysis process of industrial bioenergy productions.

scholarly journals Biological Pretreatment of Corn Stover for Enhancing Enzymatic Hydrolysis Using Thermoalkalotolerant Cellulolytic Enzymes Produced by Bacillus sp. P3

2021 ◽  
Author(s):  
Yanwen Wu ◽  
Haipeng Guo ◽  
Md. Shafiqur Rahman ◽  
Xuantong Chen ◽  
Jinchi Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Fermentation Broth ◽  
Removal Rate ◽  
Enzymatic Saccharification ◽  
Cellulolytic Enzymes ◽  
Biological Pretreatment ◽  
Hydrolysis Process ◽  
Hemicellulose Removal ◽  
Hydrolysis Of

Abstract The biological pretreatment for the enzymatic hydrolysis of lignocellulosic biomasses largely depends on an effective pretreatment process. A significant enhancement of enzymatic saccharification was obtained with corn stover using Bacillus sp. P3. The hemicellulose removal from corn stover by the strain Bacillus sp. P3 was evaluated for enhancing subsequent enzymatic hydrolysis. Therefore, our study revealed that an alkaline resistant xylanase produced by Bacillus sp. P3 in fermentation broth led to a substantially enhanced hemicellulose removal rate from corn stover within pH 9.36–9.68. However, after 20 d pretreatment of corn stover by the strain P3, the glucan content was increased by 51% and the xylan content was decreased by 35%. After 72 h of saccharification using 20 U g− 1 of commercial cellulase, the yield of reducing sugar released from 20 d pretreated corn stover was increased by 56% in comparison to the untreated corn stover. Therefore, the use of the strain P3 could be a promising approach to pretreat corn stover for enhancing the enzymatic hydrolysis process of industrial bioenergy productions.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

Reducing sugar loss in enzymatic hydrolysis of ethylenediamine pretreated corn stover

2017 ◽  
Vol 224 ◽  
pp. 405-410 ◽  
Author(s):  
Wen-Chao Li ◽  
Xia Li ◽  
Lei Qin ◽  
Jia-Qing Zhu ◽  
Xiao Han ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Reducing Sugar ◽  
Pretreated Corn Stover ◽  
Hydrolysis Of ◽  
Sugar Loss

scholarly journals Effect of Ultrasound-Assisted on Sequential Peracetic Acid and Alkaline Peroxide Pretreatment to The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch

2021 ◽  
Author(s):  
Dwini Normayulisa Putri ◽  
Meka Saima Perdani ◽  
Masafumi Yohda ◽  
Tania Surya Utami ◽  
Muhamad Sahlan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Peracetic Acid ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Empty Fruit Bunch ◽  
Hydrolysis Time ◽  
Alkaline Peroxide ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstract Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) that has been pretreated by modified pretreatment has been investigated in this study. The OPEFB used was pretreated by using sequential peracetic acid – alkaline peroxide solution. As the modification method, the assistance of pretreatment by ultrasound was conducted, in order to increase the enzyme accessibility. Therefore, it enhances the production of reducing sugar on the hydrolysis process. Prior to hydrolysis process, OPEFB was initially treated by using peracetic acid solution, comprise of CH3COOH (> 99%) and H2O2 (30% w/w), assisted by ultrasound for 3 hours at 35oC. Afterwards, OPEFB was treated by using alkaline peroxide solution, comprise of NaOH (40% w/w) and H2O2 (35% w/w), assisted by ultrasound for 10 hours at 35oC. OPEFB that has been pretreated was then subjected to enzymatic hydrolysis process using cellulase enzyme, in order to convert cellulose content into reducing sugar. Enzymatic hydrolysis was carried out at 50oC in a shaker incubator with 150 rpm for 48 hours. In this study, the effect of different enzyme concentration and hydrolysis time towards the sugar concentration in modified-pretreated OPEFB was observed and analyzed. Three different concentrations of enzyme were used, including 1.25, 2.5, and 5 g/L, and reducing sugar concentrations were analyzed at 30 and 45 minutes, and 1, 2, 4, 6, 24, 30, and 48 hours. Based on results, enzyme concentration has a significant effect to the production of reducing sugar. The reducing sugar concentrations obtained at the end of the hydrolysis process were 8.48, 11.06, 19.16 g/L, at the enzyme concentrations of 1.25, 2.5, and 5 g/L, respectively. At any hydrolysis time, the highest sugar concentration has been achieved on the highest enzyme concentration of 5 g/L. Moreover, the effective hydrolysis time were achieved at 6 hours, at all concentration of enzyme, since the production of reducing sugar were insignificant after 6 hours. This study showed an increase in reducing sugar production by 8.25% in the hydrolysis process using OPEFB pretreated by modified pretreatment compared to the non-modified pretreatment.

Acid-Alkaline Two-Stage Pretreatments of Corn Stover for Enhancing Enzymatic Saccharification

2013 ◽  
Vol 724-725 ◽  
pp. 207-211 ◽  
Author(s):  
Hai Song Wang ◽  
Hong Ling Gao ◽  
Bin Li ◽  
Xin Dong Mu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Lignocellulosic Biomass ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Alkaline Pretreatment ◽  
Two Stage ◽  
Glucose Yield ◽  
Second Stage

For the enzymatic saccharification of lignocellulosic biomass, single acid or alkaline pretreatment is not satisfactory because of the low sugar yields together with the neutralization of residual chemicals before enzymatic hydrolysis. Herein, an acid-alkaline two-stage pretreatment process was designed to treat corn stover. During the process, the pretreated liquid from the first stage and the solid residues from the second stage were mixed together for the subsequent simultaneous enzymatic hydrolysis, where a mixture of cellulase with an activity loading of 20 FPU/g substrate, cellobiase with an activity loading of 5 U/g substrate, and xylanase with an activity loading of 200 U/g substrate was used. Compared to the single acid or alkaline pretreatment, the acid-alkaline two-stage pretreatment could significantly improve the enzymatic saccharification, and 91.2% glucose yield with 52.56% of the theoretical total reducing sugar yield was achieved after the subsequent enzymatic hydrolysis.

scholarly journals IMPROVEMENT OF BIOETHANOL PRODUCTION FROM LOW GRADE AND DAMAGED LONGAN FRUITS WITH THERMAL PRETREATMENT AND DIFFERENT TYPES OF THE ENZYMATIC HYDROLYSIS

2020 ◽  
pp. 6-11
Author(s):  
TU VY THUY NGUYEN ◽  
YUWALEE UNPAPROM ◽  
PIYAPAT CHAICHOMPOO ◽  
RAMESHPRABU RAMARAJ
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Low Grade ◽  
Sugar Production ◽  
Bioethanol Production ◽  
Thermal Pretreatment ◽  
Hydrolysis Process ◽  
Different Types ◽  
Hydrolysis Of

Pretreatment is a vital step in the enzymatic hydrolysis of biomass and the successive production of bioethanol. The present study is focused on thermal pretreatment (boiling & autoclave) methods of low grade and damaged longan fruits using three different types of the enzymatic sources from commercial cellulase, an enzyme from algae and mixed enzymes (i.e., commercial cellulase with algal enzyme). Total sugar production after the hydrolysis process from commercial cellulase, the enzyme from algae and mixed enzymes were 326.41 ± 08.97 g/L, 348.68 ± 01.95 g/L and 368.42 ± 01.16 g/L, respectively. Reducing sugar after the hydrolysis process generated from commercial cellulase, the enzyme from algae and mixed enzymes was 182.54 ± 03.05 g/L, 183.33 ± 04.70 g/L and 297.78 ± 02.94 g/L, respectively. Fermentation of these hydrolysate using Saccharomyces cerevisiae TISTR 5020 produced the highest ethanol production from using commercial cellulase, the enzyme from algae and mixed enzymes was 16.74 ± 0.62 g/L, 5.38 ± 0.54 g/L and 14.32 ± 1.89 g/L, respectively. Consequently, this study suggested that suitable pretreatment and hydrolysis processes are performing a significant role in bioethanol production from low grade and damaged longan fruits.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

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