scholarly journals Effect of flavophospholipol on fecal microbiota in weaned pigs challenged with Salmonella Typhimurium

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Saranya Nair ◽  
Abdolvahab Farzan ◽  
J. Scott Weese ◽  
Zvonimir Poljak ◽  
Robert M. Friendship
Keyword(s):  
Salmonella Typhimurium ◽  
Fecal Microbiota ◽  
Weaned Pigs

scholarly journals Effect of flavophospholipol on fecal microbiota in weaned pigs challenged with Salmonella Typhimurium

2020 ◽  
Author(s):  
Saranya Nair ◽  
Vahab Farzan ◽  
J Scott Weese ◽  
Zvonimir Poljak ◽  
Robert M Friendship
Keyword(s):  
Treatment Group ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Safety Concern ◽  
Illumina Miseq ◽  
Fecal Microbiota ◽  
Control Group ◽  
Rrna Gene ◽  
Sequencing Data ◽  
Weaned Pigs

Abstract Background The heightened prevalence of Salmonella Typhimurium remains a public health and food safety concern. Studies have reported antibiotic, flavophospholipol, may have the ability to reduce Salmonella in swine, as well as alter the gut microbiota in favour of beneficial bacteria by inhibiting pathogenic bacteria. Thus, the objective of this study was to investigate the fecal microbiota of weaned pigs receiving in-feed flavophospholipol and challenged with Salmonella Typhimurium.Results Twenty-one weaned pigs were fed either a diet containing 4 ppm of flavophospholipol (treatment group) or a non-medicated feed (control group) for 36 days post-weaning (Day 1 to Day 36). The pigs were orally challenged with a 2 mL dose of 108 CFU/mL of S. Typhimurium at Day 7 and Day 8. Community bacterial DNA extracted from fecal samples collected at Day 6 (before challenge) and Day 36 (28 days after challenge) were used to assess the fecal microbiota using the V4 region of the 16S rRNA gene with Illumina MiSeq next-generation sequencing. Sequencing data were visualized using mothur and analyzed in JMP and R software. The fecal microbiota of pigs in the treatment group had differences in abundance of phyla (Firmicutes, Proteobacteria) and genera (Lactobacillus, Roseburia, Treponema, unclassified Ruminococcaceae, Blautia, Streptococcus, Megasphaera, Dorea, Sporobacter, Peptococcus, unclassified Firmicutes, Clostridium IV and Campylobacter) when compared to pigs that were controls, 28 days after challenge with Salmonella (P<0.05). Specifically, results demonstrated a significant increase in phylum Proteobacteria (P=0.001) and decrease in Firmicutes (P=0.012) and genus Roseburia (P=0.003) in the treated pigs suggestive of possible microbial dysbiosis. An increased abundance of genera Lactobacillus (P=0.012) was also noted in the treated group in comparison to the control.Conclusion Based on these findings, it is difficult to conclude whether treatment with 4 ppm of flavophospholipol is promoting favorable indigenous bacteria in the pig microbiota as previous literature has suggested.

scholarly journals Effect of flavophospholipol on fecal microbiota in weaned pigs challenged with Salmonella Typhimurium

2020 ◽  
Author(s):  
Saranya Nair ◽  
Vahab Farzan ◽  
J Scott Weese ◽  
Zvonimir Poljak ◽  
Robert M Friendship
Keyword(s):  
Treatment Group ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Safety Concern ◽  
Illumina Miseq ◽  
Fecal Microbiota ◽  
Control Group ◽  
Rrna Gene ◽  
Sequencing Data ◽  
Weaned Pigs

Abstract Background The heightened prevalence of Salmonella Typhimurium remains a public health and food safety concern. Studies have reported antibiotic, flavophospholipol, may have the ability to reduce Salmonella in swine, as well as alter the gut microbiota in favour of beneficial bacteria by inhibiting pathogenic bacteria. Thus, the objective of this study was to investigate the fecal microbiota of weaned pigs receiving in-feed flavophospholipol and challenged with Salmonella Typhimurium. Results Twenty-one weaned pigs were fed either a diet containing 4 ppm of flavophospholipol (treatment group) or a non-medicated feed (control group) for 36 days post-weaning (Day 1 to Day 36). The pigs were orally challenged with a 2 mL dose of 10 8 CFU/mL of S. Typhimurium at Day 7 and Day 8. Community bacterial DNA extracted from fecal samples collected at Day 6 (before challenge) and Day 36 (28 days after challenge) were used to assess the fecal microbiota using the V4 region of the 16S rRNA gene with Illumina MiSeq next-generation sequencing. Sequencing data were visualized using mothur and analyzed in JMP and R software. The fecal microbiota of pigs in the treatment group had differences in abundance of phyla (Firmicutes, Proteobacteria) and genera ( Lactobacillus, Roseburia , Treponema, unclassified Ruminococcaceae, Blautia , Streptococcus , Megasphaera , Dorea , Sporobacter , Peptococcus , unclassified Firmicutes, Clostridium IV and Campylobacter) when compared to pigs that were controls, 28 days after challenge with Salmonella ( P <0.05). Specifically, results demonstrated a significant increase in phylum Proteobacteria ( P= 0.001) and decrease in Firmicutes ( P =0.012) and genus Roseburia ( P =0.003) in the treated pigs suggestive of possible microbial dysbiosis. An increased abundance of genera Lactobacillus ( P =0.012) was also noted in the treated group in comparison to the control. Conclusion Based on these findings, it is difficult to conclude whether treatment with 4 ppm of flavophospholipol is promoting favorable indigenous bacteria in the pig microbiota as previous literature has suggested.

scholarly journals Effect of flavophospholipol on fecal microbiota in weaned pigs challenged with Salmonella Typhimurium

2019 ◽  
Author(s):  
Saranya Nair ◽  
Vahab Farzan ◽  
J Scott Weese ◽  
Zvonimir Poljak ◽  
Robert M Friendship
Keyword(s):  
Treatment Group ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Safety Concern ◽  
Illumina Miseq ◽  
Fecal Microbiota ◽  
Control Group ◽  
Rrna Gene ◽  
Sequencing Data ◽  
Weaned Pigs

Abstract Background The heightened prevalence of Salmonella Typhimurium in nursery pigs remains a public health and food safety concern. Studies have reported antibiotic, flavophospholipol, may have the ability to reduce Salmonella in swine, as well as alter the gut microbiota in favour of beneficial bacteria by inhibiting pathogenic bacteria. Thus, the objective of this study was to investigate the fecal microbiota of weaned pigs receiving in-feed flavophospholipol and challenged with Salmonella Typhimurium. Results Twenty-one weaned pigs were fed either a diet containing 4 ppm of flavophospholipol (treatment group) or a non-medicated feed (control group) for 36 days post-weaning (Day 1 to Day 36). The pigs were orally challenged with a 2 mL dose of 10 8 CFU/mL of S. Typhimurium at Day 7 and Day 8. Community bacterial DNA extracted from fecal samples collected at Day 6 (before challenge) and Day 36 (28 days after challenge) were used to assess the fecal microbiota using the V4 region of the 16S rRNA gene with Illumina MiSeq next-generation sequencing. Sequencing data were visualized using mothur, and analyzed in JMP and R. The fecal microbiota of pigs in treatment group had significant differences in abundance of phyla (Firmicutes, Proteobacteria) and genera ( Lactobacillus, Roseburia , Treponema, unclassified Ruminococcaceae, Blautia , Streptococcus , Megasphaera , Dorea , Sporobacter , Peptococcus , unclassified Firmicutes, Clostridium IV and Campylobacter) when compared to pigs that controls 28 days after challenge with Salmonella ( P <0.05). Specifically, results demonstrated a significant increase in phylum Proteobacteria ( P= 0.001) and decrease in Firmicutes ( P =0.012) and genus Roseburia ( P =0.003) in the treated pigs suggestive of possible microbial dysbiosis. An increased abundance of genera Treponema ( P =0.012) and Lactobacillus ( P =0.012) was also noted in the treated group in comparison to the control. Conclusion Based on these findings, it is difficult to conclude whether treatment with 4 ppm of flavophospholipol is aiding in reducing Salmonella and promoting favorable indigenous bacteria in the pig microbiota as previous literature has suggested.

Nose-to-nose transmission of Salmonella Typhimurium between weaned pigs

2007 ◽  
Vol 125 (3-4) ◽  
pp. 355-361 ◽  
Author(s):  
Celso J.B. Oliveira ◽  
Thiago B. Garcia ◽  
Luiz Fernando O.S. Carvalho ◽  
Patrícia E.N. Givisiez
Keyword(s):  
Salmonella Typhimurium ◽  
Weaned Pigs

Development of a Salmonella Typhimurium challenge model in weaned pigs to evaluate effects of water and feed interventions on fecal shedding and growth performance1

2017 ◽  
Vol 95 (7) ◽  
pp. 2879-2890 ◽  
Author(s):  
P. J. van der Wolf ◽  
J. G. M. Wientjes ◽  
A. E. Heuvelink ◽  
A. M. B. Veldhuis ◽  
H. M. J. van Hees ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Weaned Pigs ◽  
Fecal Shedding

scholarly journals Effect of benzoic acid in the feed on Salmonella typhimurium in weaned pigs

2005 ◽  
Author(s):  
Charlotte S. Kristensen ◽  
Lisbeth Jørgensen ◽  
Jaap Boes
Keyword(s):  
Benzoic Acid ◽  
Salmonella Typhimurium ◽  
Weaned Pigs

scholarly journals Influence of light exposure on horizontal transmission of Salmonella typhimurium in weaned pigs

2007 ◽  
Author(s):  
T. Edrington ◽  
T. Callaway ◽  
K. J. Genovese ◽  
Robin C. Anderson ◽  
David J. Nisbet
Keyword(s):  
Salmonella Typhimurium ◽  
Light Exposure ◽  
Horizontal Transmission ◽  
Weaned Pigs

scholarly journals Functional amino acid supplementation, regardless of dietary protein content, improves growth performance and immune status of weaned pigs challenged with Salmonella Typhimurium

2021 ◽  
Vol 99 (2) ◽  
Author(s):  
Lucas A Rodrigues ◽  
Michael O Wellington ◽  
J Caroline González-Vega ◽  
John K Htoo ◽  
Andrew G Van Kessel ◽  
...  
Keyword(s):  
Body Temperature ◽  
Serum Albumin ◽  
Salmonella Typhimurium ◽  
Growth Performance ◽  
Protein Content ◽  
Dietary Protein ◽  
Saline Solution ◽  
Immune Status ◽  
Weaned Pigs ◽  
Fecal Score

Abstract High dietary protein may increase susceptibility of weaned pigs to enteric pathogens. Dietary supplementation with functional amino acids (FAA) may improve growth performance of pigs during disease challenge. The objective of this study was to evaluate the interactive effects of dietary protein content and FAA supplementation above requirements for growth on performance and immune response of weaned pigs challenged with Salmonella. Sixty-four mixed-sex weanling pigs (13.9 ± 0.82 kg) were randomly assigned to dietary treatments in a 2 × 2 factorial arrangement with low (LP) or high protein (HP) content and basal (AA–) or FAA profile (AA+; Thr, Met, and Trp at 120% of requirements) as factors. After a 7-d adaptation period, pigs were inoculated with either a sterile saline solution (CT) or saline solution containing Salmonella Typhimurium (ST; 3.3 × 109 CFU/mL). Growth performance, body temperature, fecal score, acute-phase proteins, oxidant/antioxidant balance, ST shedding score in feces and intestinal colonization, fecal and digesta myeloperoxidase (MPO), and plasma urea nitrogen (PUN) were measured pre- and postinoculation. There were no dietary effects on any measures pre-inoculation or post-CT inoculation (P &gt; 0.05). Inoculation with ST increased body temperature and fecal score (P &lt; 0.05), serum haptoglobin, plasma superoxide dismutase (SOD), malondialdehyde (MDA), PUN, and fecal MPO, and decreased serum albumin and plasma reduced glutathione (GSH):oxidized glutathione (GSSG) compared with CT pigs (P &lt; 0.05). ST-inoculation reduced average daily gain (ADG) and feed intake (ADFI) vs. CT pigs (P &lt; 0.05) but was increased by AA+ vs. AA– in ST pigs (P &lt; 0.05). Serum albumin and GSH:GSSG were increased while haptoglobin and SOD were decreased in ST-inoculated pigs fed AA+ vs. AA– (P &lt; 0.05). PUN was higher in HP vs. LP-fed pigs postinoculation (P &lt; 0.05). Fecal ST score was increased in ST-inoculated pigs on days 1 and 2 postinoculation and declined by day 6 (P &lt; 0.05) in all pigs while the overall score was reduced in AA+ vs. AA– pigs (P &lt; 0.05). Cecal digesta ST score was higher in HP vs. LP-fed pigs and were lower in AA+ compared with AA– fed pigs in the colon (P &lt; 0.05). Fecal and digesta MPO were reduced in ST pigs fed AA+ vs. AA– (P &lt; 0.05). These results demonstrate a positive effect of FAA supplementation, with minimal effects of dietary protein, on performance and immune status in weaned pigs challenged with Salmonella.

Efficacy of Bacillus subtilis RX7 and Bacillus methylotrophicus C14 as probiotics on growth performance, digestibility, blood profile and fecal microbiota in weaned pigs.

2018 ◽  
Author(s):  
Santi Devi Upadhaya ◽  
Xin Jian Lei ◽  
Subin Serpunja ◽  
In Ho Kim
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Feed Intake ◽  
Basal Diet ◽  
Fecal Microbiota ◽  
Weaned Pigs ◽  
Blood Profile ◽  
Bacillus Methylotrophicus ◽  
Dietary Treatments

A total of 60 weaned piglets (28-day old; [Landrace ×Yorkshire]×Duroc) were used to evaluate the efficacy of Bacillus-based probiotic on performance, digestibility, blood profiles, and fecal microbiota in weaned pigs. The piglets were randomly allotted to three treatments with 4 pens per treatment and 5 pigs per pen. Dietary treatments consisted of: CON, basal diet; BS, basal diet + 0.1% of Bacillus subtilis RX7; BM, basal diet + 0.1% of Bacillus methylotrophicus C14. The average daily feed intake (ADFI) was reduced (P=0.01) in BS and BM treatments compared with CON. The apparent total tract digestibility of energy was increased (P less than 0.05) in BS and BM compared with CON on day 14. The supplementation of probiotics led to increase (P less than 0.05) in fecal lactic acid bacteria counts on day 42. In conclusion, probiotic supplementation reduced feed intake, increased energy digestibility and lactic acid bacteria counts and numerically reduced Salmonella counts in weaned pigs.

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