scholarly journals Designing an ideal alcohol-based hand sanitizer: in vitro antibacterial responses of ethanol and isopropyl alcohol solutions to changing composition

AAPS Open ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ifeanyi T. Nzekwe ◽  
Onyedika I. Agwuka ◽  
Moses U. Okezie ◽  
Daniel O. Fasheun ◽  
Petra O. Nnamani ◽  
...  
Keyword(s):  
Isopropyl Alcohol ◽  
Electrolyte Concentration ◽  
Aloe Vera ◽  
Ammonium Compound ◽  
Herbal Extracts ◽  
Time Intervals ◽  
In Vitro Methods ◽  
Hand Sanitizer ◽  
Formulation Factors

AbstractThis study aimed to achieve an in vitro quantification of the effects of composition and formulation factors on the killing rates of alcohol-based hand sanitizers. The killing rates of 85% ethyl alcohol (ET) and isopropyl alcohol (IPA) were studied under different conditions such as pH, electrolyte concentration, or inclusion of herbal extracts (cucumber, carrot, and aloe vera), a quaternary ammonium compound, or thickener over different time intervals. Changes in the activities were retested after 3 months as an indication of stability. From two-way ANOVA, both the time of exposure and the sanitizer type affected the activity against Staphylococcus aureus (P = 0.001 for both alcohols), whereas for Escherichia coli, time of exposure was significant (P = 0.027), while sanitizer type was less significant (P = 0.063). Extreme pHs, the presence of ions, and the inclusion of additives such as benzalkonium chloride (BAC), plant extracts, or carbomer impacted the 3-month activity of the samples differently. Important differences existing in the activities of ET and IPA, as a function of formulation factors or use conditions have been quantified using in vitro methods. Formulations should best be tailored for particular purposes and the all-purpose hand sanitizer may not exist. Graphic Abstract

Comparison of antibacterial efficacy of herbal extracts of aloe vera leaves and mushroom against streptococcus mutansand lactobacillus - an in vitro study

2017 ◽  
Vol 9 (1) ◽  
pp. 23-29
Author(s):  
Bijo Kurian
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Aloe Vera ◽  
In Vitro Study ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Group Iii ◽  
Mushroom Extract ◽  
Group I

BACKGROUND AND OBJECTIVES: This study evaluated and compared the antimicrobial efficacy and minimal inhibitory concentration (MIC) of chlorhexidine 0.12% and extracts of aloe vera and mushroom against Streptococcus mutans and lactobacillus. MATERIALS & METHODOLOGY: The agar disc diffusion and broth micro dilution method was used to check the antimicrobial activity of 0.12% chlorhexidine and aqueous extracts of two medicinal plants. The test samples were divided as follows- Streptococcus mutans, group I: chlorhexidine, group II: Aloe vera and group III: Mushroom. Lactobacillus, Group I: chlorhexidine, group II: Aloe vera extract and group III: Mushroom extract. The zone of inhibition and MIC values were tabulated and the data was statistically analyzed using ANOVA and Bonferroni post- hoc tests. RESULTS: Chlorhexidine shows maximum antibacterial action against Streptococcus mutans and lactobacillus followed by mushroom extract and aloe veraextracts with the zone of inhibition of (20.3mm/ 24.13), (17.03/ 16.05), (14.09/14.26) respectively. Both the extracts had MIC value of 80µg/ ml and chlorhexidine was 40µg/ml. CONCLUSION: Within the limitations of this in- vitro study, it can be concluded that all the herbal extracts tested in this study demonstrated antibacterial activity against MS and lactobacillus. Chlorhexidine showed maximum antibacterial action against Streptococcus mutans and lactobacillus followed by mushroom extract and then aloe veraextracts with the zone of inhibition of (20.3mm/24.13), (17.03/ 16.05), (14.09/14.26) respectively. Herbal products have potent antimicrobial activity that can be looked at as an alternative to chlorhexidine. However, further in vitro and long-term in vivo studies are recommended to confirm and correlate the findings of this study clinically.

In vitro evaluation of the effect of selected herbal extracts on drug transport across porcine jejunal tissue

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
C Tarirai ◽  
A Viljoen ◽  
P Chinyemba ◽  
A Khatib ◽  
P Jiyane ◽  
...  
Keyword(s):  
Drug Transport ◽  
Herbal Extracts ◽  
In Vitro Evaluation

Anti-amnesic activity screening of the seed ethanol extracts of Turkish Paeonia taxa by in vitro methods

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
D Sevim ◽  
FS Senol ◽  
I Orhan ◽  
B Şener ◽  
E Kaya
Keyword(s):  
In Vitro Methods ◽  
Activity Screening ◽  
Ethanol Extracts

In vitro strategy to quantify changes in hepatobiliary drug clearance by herbal extracts

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
JP Jackson ◽  
K Freeman ◽  
J Hatfield ◽  
B St Claire ◽  
C Hubert ◽  
...  
Keyword(s):  
Drug Clearance ◽  
Herbal Extracts

Analysis of antidepressant properties of some Papaver species by in vivo and in vitro methods

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
OML Bayazeid ◽  
F Yalcin ◽  
M İlhan ◽  
H Karahan ◽  
E Kupeli-Akkol ◽  
...  
Keyword(s):  
In Vitro Methods

In vitro study of the interaction between human gut microbiota and herbal extracts using willow bark extract as an example

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EM Pferschy-Wenzig ◽  
K Koskinen ◽  
C Moissl-Eichinger ◽  
R Bauer
Keyword(s):  
Gut Microbiota ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bark Extract ◽  
Herbal Extracts ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Willow Bark

In Vitro Incorporation of Sodium Acetate-l-C14 into Leukocyte Lipids of Normal and Leukaemic Subjects as a Function of Incubation Time

1962 ◽  
Vol 02 (02) ◽  
pp. 165-172
Author(s):  
C Miras ◽  
G Lewis ◽  
J Mantzos
Keyword(s):  
Sodium Acetate ◽  
Linear Part ◽  
Lipid Synthesis ◽  
Normal Subjects ◽  
Cytostatic Agents ◽  
Time Intervals ◽  
Total Blood ◽  
Effect Of Treatment ◽  
Product Relation

Summary1. Separated leukocytes or total blood from normal subjects, untreated leukaemic patients and from leukaemic patients treated with cytostatic agents were incubated with CH3COONa-l-C14. Radioactivity of mixed lipids was measured at standard time intervals.2. The time incorporation curve observed with leukocytes from treated leukaemic patients showed after an initial linear part, a more rapid levelling off than the curves observed with leukocytes from untreated and normal subjects.3. Therefore, an indirect effect of treatment on leukocyte lipid synthesis seems to be present.4. Phospholipid and neutral lipid synthesis by leukaemic leukocytes was also studied. The results give no evidence that these fractions as a whole have any precursor-product relation.

The Effects of Some Synthetic Compounds on In Vitro Fibrinolytic Activity Measured by Different Methods and the Relevance to Activity In Vivo

1972 ◽  
Vol 28 (03) ◽  
pp. 351-358
Author(s):  
A.J Baillie ◽  
A. K Sim
Keyword(s):  
Inhibitory Activity ◽  
Substrate Concentration ◽  
Fibrinolytic Activity ◽  
Synthetic Compounds ◽  
In Vitro Methods ◽  
Narrow Concentration Range

SummaryThe activity of several synthetic compounds, rated from good to poor (or inactive) fibrinolytic activators, has been assessed by two different commonly-used in vitro methods. Compounds shown to be active over a narrow concentration range in the hanging clot test were shown to be inhibitors of plasmin and trypsin in the casein-olytic test. The inhibitory activity of these compounds was shown to increase with increasing substrate concentration and apparent activity in the hanging clot test. Possible explanations and relevance of these observations are discussed.

Sign in / Sign up

Export Citation Format

Share Document