scholarly journals Biological control of poplar anthracnose caused by Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Huayi Huang ◽  
Chengming Tian ◽  
Yonghuai Huang ◽  
Huanhua Huang
Keyword(s):  
Biological Control ◽  
Colletotrichum Gloeosporioides

scholarly journals First Report of Anthracnose of Salsola tragus Caused by Colletotrichum gloeosporioides in Greece

Plant Disease ◽  
2006 ◽  
Vol 90 (7) ◽  
pp. 971-971 ◽  
Author(s):  
D. K. Berner ◽  
C. A. Cavin ◽  
M. B. McMahon ◽  
I. Loumbourdis
Keyword(s):  
Biological Control ◽  
Colletotrichum Gloeosporioides ◽  
Type A ◽  
The United States ◽  
Ionic Surfactant ◽  
Potential Candidate ◽  
Conidial Suspension ◽  
Type B ◽  
First Report ◽  
Stems And Leaves

In early October of 2005, dying Salsola tragus L. (Russian thistle, tumbleweed), family Chenopodiaceae, plants were found along the Aegean Sea at Kryopigi Beach, Greece (40°02′29″N, 23°29′02″E, elevation 0 m). All of the 30 to 40 plants in the area were diseased and approximately 80% were dead or dying. All plants were relatively large (approximately 1 m tall × 0.5 m diameter), and living portions of diseased plants were flowering. Dying plants had irregular, necrotic lesions extending the length of the stems. Leaves of these plants were also necrotic. Lesions on stems and leaves were dark brown and usually coalesced. Diseased stem pieces were taken to the European Biological Control Laboratory, USDA, ARS at the American Farm School in Thessaloniki, Greece. There, diseased stem pieces were surface disinfested for 15 min with 0.5% NaOCl and placed on moist filter paper in petri dishes. Numerous, waxy subepidermal acervuli with black setae were observed in all lesions after 2 to 3 days. Conidiophores were simple, short, and erect. Conidia were one-celled, hyaline, ovoid to oblong, falcate to straight, 12.9 to 18.0 × 2.8 to 5.5 μm (mode 16.1 × 4.5 μm). These characters conformed to the description of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. (2). Conidia were placed on modified potato carrot agar and axenic cultures from these isolations were sent to the quarantine facility of the Foreign Disease-Weed Science Research Unit, USDA, ARS, Fort Detrick, MD for testing. On the basis of DNA sequences, two variants within S. tragus have been described in California and named “Type A” and “Type B” (1). Conidia were harvested from 14-day-old cultures grown on 20% V8 juice agar, and healthy stems and leaves of 18 30-day-old plants of S. tragus Type A and 10 Type B plants were spray inoculated with an aqueous conidial suspension (1.0 × 106 conidia/ml plus 0.1% non-ionic surfactant). Three control plants of each type were sprayed with water and surfactant only. Plants were placed in an environmental chamber (18 h of dew in darkness at 25°C). After 1 day, all plants were transferred to a greenhouse (20 to 25°C, 30 to 50% relative humidity, and natural light augmented with 12-h light periods with 500-W sodium vapor lights). Lesions developed on stems of inoculated Type A plants after 5 days. After 14 days, all inoculated Type A plants were dead. Lesions on Type B plants were small and localized; all plants were diseased but no plants died. No symptoms occurred on control plants. C. gloeosporioides was reisolated 14 to 21 days after inoculation from stem pieces of all inoculated plants of both types of S. tragus. This isolate of C. gloeosporioides is a destructive pathogen on S. tragus Type A and is a potential candidate for biological control of this weed in the United States. To our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on S. tragus in Greece. A voucher specimen has been deposited with the U.S. National Fungus Collections, Beltsville, MD (BPI 871126). Nucleotide sequences for the internal transcribed spacers (ITS 1 and 2) were deposited in GenBank (Accession No. DQ344621) and exactly matched sequences of the teleomorph, Glomerella cingulata. References: (1) F. Ryan and D. Ayres. Can. J. Bot. 78:59, 2000. (2) B. C. Sutton. Page 15 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International Mycological Institute, Wallingford, UK, 1992.

scholarly journals BIOLOGICAL CONTROL OF Colletotrichum gloeosporioides IN PEPPER WITH ISOLATES OF Bacillus subtilis

2018 ◽  
Vol 93 (2) ◽  
pp. 195
Author(s):  
Jeniffer Kelly Cortes Amaro ◽  
Bruno Sérgio Vieira ◽  
Luciana Alves Sousa
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Colletotrichum Gloeosporioides

Objetivou-se estudar o potencial antagônico de isolados de Bacillus subtilis a Colletotrichum gloeosporioides, agente causal da antracnose em frutos de pimenta. Foram estudados 21 isolados de Bacillus subtilis quanto a capacidade de inibir o desenvolvimento do fitopatógeno nas seguintes metodologias in vitro: cultivo pareado, metabólitos produzidos pelos isolados, influência da inoculação conjunta e controle da antracnose in vivo (frutos destacados de pimenta). Os isolados BSV-11, BSV-05, BSV-12, BSV-17, BSV-07, BSV-16, BSV-09, BSV-20, BSV-13 e BSV-18 foram promissores como potenciais antagonistas a C. gloeosporioides, com evidente formação de halo de inibição, para a metodologia do cultivo pareado. Estes isolados produziram metabólitos termoestáveis contra C. gloeosporioides evidenciando ser antibiose o mecanismo antagônico envolvido na supressão do patógeno. O contato direto dos isolados bacterianos com o patógeno inibiu totalmente o crescimento micelial de C. gloeosporioides, exceto os isolados BSV-20 e BSV-09. Os isolados BSV-20, BSV-18, BSV-17, BSV-07 e BSV-12 inibiram significativamente infecções de antracnose em frutos de pimenta em pós-colheita.

scholarly journals Biological Control of Anthracnose (Colletotrichum gloeosporioides) in Red Pepper by Bacillus sp. CS-52

2014 ◽  
Vol 50 (3) ◽  
pp. 201-209 ◽  
Author(s):  
Joung-Ja Kwon ◽  
Jung-Bok Lee ◽  
Beam-Soo Kim ◽  
Eun-Ho Lee ◽  
Kyeong-Muk Kang ◽  
...  
Keyword(s):  
Biological Control ◽  
Colletotrichum Gloeosporioides ◽  
Red Pepper ◽  
Bacillus Sp

Biological Control of Northern Jointvetch in Rice by An Endemic Fungal Disease

Weed Science ◽  
1973 ◽  
Vol 21 (4) ◽  
pp. 303-307 ◽  
Author(s):  
J. T. Daniel ◽  
G. E. Templeton ◽  
R. J. Smith ◽  
W. T. Fox
Keyword(s):  
Biological Control ◽  
Oryza Sativa ◽  
Glycine Max ◽  
Gossypium Hirsutum ◽  
Incubation Period ◽  
Colletotrichum Gloeosporioides ◽  
Vegetable Crops ◽  
Liquid Media ◽  
Water Sprays ◽  
Common Field

An endemic anthrocnose disease of northern jointvetch [Aeschynomene virginica(L.) B.S.P.] incited by the fungusColletotrichum gloeosporioides(Penz.) Sacc. f. sp.aeschynomenewas discovered in 1969 at Stuttgart, Arkansas. When grown on solid or in liquid media, the organism grew rapidly and sporulated abundantly. From 1970 through 1972 water sprays of the fungus spores at 2 to 15 million spores per milliliter in 94 to 374 L/ha controlled northern jointvetch grown in the growth chamber, greenhouse, and field. The fungus controlled plants ranging from 5 to 66 cm tall. The disease developed on northern jointvetch most rapidly at temperatures of 23 to 32 C and at relative humidities above 80%. An incubation period of 4 to 7 days and up to 5 weeks was usually required to kill the weeds. The fungus was specific forAeschynomenespecies since it was very virulent onA. virginicaand only slightly virulent on Indian jointvetch (A. indicaL.). It did not affect rice (Oryza sativaL.), soybeans [Glycine max(L.) Merr.], cotton (Gossypium hirsutumL.), or 12 other common field, forage, and vegetable crops or 15 common weeds.

scholarly journals First Report of Glomerella cingulata (Colletotrichum gloeosporioides) Causing Anthracnose and Tip Dieback of Lygodium microphyllum and L. japonicum in Australia

Plant Disease ◽  
2008 ◽  
Vol 92 (9) ◽  
pp. 1369-1369 ◽  
Author(s):  
K. B. Ireland ◽  
N. A. Haji Mohamad Noor ◽  
E. A. B. Aitken ◽  
S. Schmidt ◽  
J. C. Volin
Keyword(s):  
Biological Control ◽  
Colletotrichum Gloeosporioides ◽  
Biological Control Agent ◽  
Direct Sequencing ◽  
Pathogenic Fungi ◽  
In Planta ◽  
Glomerella Cingulata ◽  
Isolation And Identification ◽  
First Report ◽  
Introduced Range

The Old World climbing fern, Lygodium microphyllum (Cav.) R. Br., and Japanese climbing fern, L. japonicum (Thunb.) Sw., are invasive noxious weeds in Florida (1). Exploratory surveys for classical biological control agents of L. microphyllum in the fern's native range of Australia and Asia have focused on aboveground herbivores (1). From February to August 2006, fungi were isolated from symptomatic foliage, including lesions associated with leaf curls caused by the mite Flocarus perrepae Knihinicki & Boczek., obtained from L. microphyllum at sites across southeast Queensland, Australia and from both fern species grown at the CSIRO Long Pocket Laboratories in Brisbane, Australia. Anthracnose symptoms with chlorotic margins, initiating at the tip or base of the individual pinnules, were observed on fronds. Dieback symptoms affected growing tips, with sunken lesions and a gradual necrotic wilt as far as the next growth junction of pinnae. Sections from diseased margins were surface sterilized, placed onto water agar, and incubated at 23°C with a 16-h photoperiod. Variable colonies of white-to-gray mycelia, felted or tufted with complete margins, grew well on oatmeal agar and potato dextrose agar. Conidia were hyaline to light salmon, aseptate, straight, and cylindrical (10.4 to 18.2 × 2.6 to 5.2 μm), borne in salmon-to-bright orange masses at 25°C, and consistent with previous descriptions of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. (3), anamorph of Glomerella cingulata (2). Asci that formed after 3 to 4 weeks in culture were eight-spored, clavate to cylindrical (46.8 to 62.4 × 9.1 to 11.7 μm), and thickened at the apex, and ascospores were cylindrical (11.7 to 18.2 × 3.9 to 5.2 μm), slightly curved, unicellular and hyaline, which is consistent with descriptions of G. cingulata (2). No fruiting bodies were observed in planta; acervuli, setae, and perethecia were not observed. Identification was further confirmed by molecular analysis using the primer pair ITS1/ITS4 (4) (GenBank Accession No. EU697014), indicating 100% similarity to isolates of G. cingulata. To confirm pathogenicity, Koch's postulates were performed on three plants of L. japonicum and 12 plants of L. microphyllum, with an equal number of controls. Conidial suspensions were made to 1.7 × 106 conidia ml–1. During the experiments in the glasshouse, temperatures ranged from 12.6 to 40°C and relative humidity from 39 to 85%. Tips and fronds were collected after 2 to 8 weeks and isolation and identification performed. G. cingulata was consistently reisolated from diseased tissue. No symptoms appeared on controls and isolations did not yield the pathogen. To our knowledge, this is the first report of G. cingulata infecting L. microphyllum and L. japonicum in Australia. Its potential as a biological control agent in the ferns' introduced range remains to be tested. References: (1) J. A. Goolsby et al. Biol. Control. 28:33, 2003. (2) J. E. M. Mordue. Glomerella cingulata. No. 315 in: CMI Descriptions of Pathogenic Fungi and Bacteria. CAB, Kew, UK, 1971. (3) B. C. Sutton. The Genus Glomerella and its Anamorph Colletotrichum. In: Colletotrichum: Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK, 1992. (4) T. M. White et al. Amplification and Direct Sequencing of Fungal Ribosomal RNA for Phylogenetics. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, San Diego, 1990.

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