Carbendazim-induced haematological, biochemical and histopathological changes to the liver and kidney of male rats

2001 ◽  
Vol 20 (12) ◽  
pp. 625-630 ◽  
Author(s):  
G Selmanoğlu ◽  
N Barlas ◽  
S Songür ◽  
E A Koçskaya
Keyword(s):  
Mononuclear Cell ◽  
Biochemical Parameters ◽  
Kidney Tissue ◽  
Male Rats ◽  
Cell Infiltration ◽  
Mononuclear Cell Infiltration ◽  
Hydropic Degeneration ◽  
Wide Range ◽  
Liver And Kidney ◽  
Mean Cell Hemoglobin

Carbendazim is a systemic broad-spectrum fungicide controlling a wide range of pathogens. It is also used as a preservative in paint, textile, papermaking and leather industry, as well as a preservative of fruits. In the present study, carbendazim was administered at 0, 150, 300 and 600 mg/kg per day doses orally to male rats (Rattus rattus) for 15 weeks. At the end of the experiment, blood samples, liver and kidney tissues of each animal were taken. Serum enzyme activities, and haematological and biochemical parameters were analysed. In toxicological tests, 600 mg/kg per day doses of carbendazim caused an increase of albumin, glucose, creatinine and cholesterol levels. Also, at the same doses, white blood cell and lymphocyte counts decreased. However, mean cell hemoglobin and mean cell hemoglobin concentrations increased. Histopathological examinations revealed congestion, an enlargement of the sinusoids, an increase in the number of Kupffer cells, mononuclear cell infiltration and hydropic degeneration in the liver. At the highest doses, congestion, mononuclear cell infiltration, tubular degeneration and fibrosis were observed in the kidney tissue. These results indicate that 300 and 600 mg/kg per day carbendazim affected the liver and kidney tissue and caused some changes on haematological and biochemical parameters of rats.

Comprehensive morphometric analysis of mononuclear cell infiltration during experimental renal allograft rejection

2013 ◽  
Vol 28 (1) ◽  
pp. 24-31 ◽  
Author(s):  
Ute Hoffmann ◽  
Tobias Bergler ◽  
Bettina Jung ◽  
Andreas Steege ◽  
Claudia Pace ◽  
...  
Keyword(s):  
Mononuclear Cell ◽  
Morphometric Analysis ◽  
Allograft Rejection ◽  
Renal Allograft ◽  
Cell Infiltration ◽  
Mononuclear Cell Infiltration ◽  
Renal Allograft Rejection

scholarly journals Human interferon-inducible protein-10 induces mononuclear cell infiltration in mice and promotes the migration of human T lymphocytes into the peripheral tissues and human peripheral blood lymphocytes-SCID mice

Blood ◽  
1996 ◽  
Vol 87 (4) ◽  
pp. 1423-1431 ◽  
Author(s):  
DD Taub ◽  
DL Longo ◽  
WJ Murphy
Keyword(s):  
T Cell ◽  
Mononuclear Cell ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Cell Infiltration ◽  
Mononuclear Cell Infiltration ◽  
Human T Cell ◽  
Inducible Protein ◽  
Interferon Inducible Protein

The human cytokine, interferon-inducible protein-10 (IP-10), is a small glycoprotein secreted by activated monocytes, T cells, keratinocytes, astrocytes, and endothelial cells and is structurally related to the alpha subfamily of chemotactic cytokines called chemokines (Taub and Oppenheim, Cytokine 5:175, 1993). However, in contrast to other alpha chemokines that induce neutrophil migration, IP-10 has been shown to chemoattract monocytes and T lymphocytes in vitro, suggesting a role in T-cell-mediated immune responses. We therefore examined the effects of human IP-10 after in vivo administration. IP-10 induces significant mononuclear cell infiltration after subcutaneous injections in normal mice. In an effort to study the in vivo effects of IP-10 on human leukocyte migration, we then examined the ability of recombinant human IP-10 (rhIP-10) to induce human-T-cell infiltration using a human/severe combined immune deficiency (SCID) mouse model. SCID mice received an intraperitoneal injection of human peripheral blood lymphocytes (10(8) cells), followed by a subcutaneous injection of rhIP- 10 (1 micrograms/injection) in the hind flank for 4 hours or sequential injections for 3 days. The skin and underlying tissue from the rhIP-10 injection site were then biopsied and examined for the extent of mononuclear cell infiltration. rhIP-10 again induced significant mononuclear cell accumulation 72 hours after injection. Immunohistologic evaluation determined that a significant number of human CD3+ T cells were recruited in response to rhIP-10 injections. These results show that rhIP-10 is capable of inducing human T-cell migration in vivo and may play an important role in monocyte and lymphocyte recruitment into inflammatory sites.

scholarly journals Multifaceted Assessment of Chronic Gastritis: A Study of Correlations between Serological, Endoscopic, and Histological Diagnostics

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Toshitatsu Takao ◽  
Takeshi Ishikawa ◽  
Takashi Ando ◽  
Madoka Takao ◽  
Tsuguhiro Matsumoto ◽  
...  
Keyword(s):  
Mononuclear Cell ◽  
Chronic Gastritis ◽  
Classification System ◽  
High Accuracy ◽  
Test Method ◽  
Cell Infiltration ◽  
Mononuclear Cell Infiltration ◽  
Pepsinogen I ◽  
Gastric Corpus ◽  
Corpus Gastritis

Aim. Chronic gastritis was assessed serologically, endoscopically and histologically to identify correlations between these methods.Methods. Subjects comprised 319 patients who had provided informed consent. Serological assessment of chronic gastritis was based on the pepsinogen test method. Endoscopic gastritis and histological gastritis were assessed and scored according to the Kimura-Takemoto classification system and the updated Sydney classification system respectively, and correlations between these three methods were studied.Results. Pepsinogen I/II ratio showed a significant correlation to the extent of mononuclear cell infiltration of the gastric corpus. When histological gastritis was divided, on the basis of the distribution of mononuclear cell infiltration, into gastritis limited to the antrum and corpus gastritis, these types were distinguished with high accuracy using a pepsinogen I/II ratio of 3 as the cutoff. A good correlation was also seen between pepsinogen I/II ratio and development of atrophy in endoscopic gastritis, where groups with and without advanced atrophy were also distinguished with high accuracy using a cutoff value of 3.Conclusion. Significant correlations exist between serum pepsinogen levels, endoscopic gastritis, and histological gastritis. Pepsinogen I/II ratio allows prediction of the existence of endoscopic gastritis and histological gastritis, or the extent of their development, with high accuracy.

Intercellular Adhesion Molecule 1 Mediates Mononuclear Cell Infiltration into Rat Glomeruli after Renal Ablation

Nephron ◽  
1998 ◽  
Vol 79 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Nobuyuki Miyatake ◽  
Kenichi Shikata ◽  
Hikaru Sugimoto ◽  
Masahiko Kushiro ◽  
Yasushi Shikata ◽  
...  
Keyword(s):  
Mononuclear Cell ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Cell Infiltration ◽  
Intercellular Adhesion Molecule ◽  
Mononuclear Cell Infiltration ◽  
Intercellular Adhesion Molecule 1 ◽  
Renal Ablation

Ameliorative Effect of Natural and Synthetic Antioxidants on Arsenic Toxicity in Male Mice: Biochemical and Histological Perspectives

2018 ◽  
Author(s):  
Sayed Amer ◽  
Yousif Al-Zahrani ◽  
Mohammad AL-Harbi
Keyword(s):  
Mononuclear Cell ◽  
Lethal Dose ◽  
Treated Group ◽  
Blood Parameters ◽  
Protective Role ◽  
Cell Infiltration ◽  
Arsenic Toxicity ◽  
Protective Effects ◽  
Mononuclear Cell Infiltration ◽  
Sodium Arsenate

The present study aimed to investigate the protective effects of some natural and artificial antioxidants on the hepato-renal injuries induced by arsenic toxicity. Sixty adult male albino mice weighing 30-40 g were subjected to a sub-lethal dose of sodium arsenate (40 mg/kg body weight) to investigate hematological, biochemical and histopathological alterations resulting from arsenic-induced hepato-renal toxicity. Arsenic-exposed mice were also co-treated with different antioxidants including green tea, garlic and vitamin C to reveal their potential protective role. The antioxidants induced normalization of all blood parameters that showed significant declines by arsenic toxicity. ALT and AST activities were significantly increased in sodium arsenate treated group compared to all other groups. These enzymes did not acquire insignificant differences in antioxidants-treated groups compared to the control mice. Creatinine and urea were significantly increased in arsenate treated mice and become normal in mice co-treated with different antioxidants. Liver sections of arsenate treated mice showed venous congestion, sinusoidal dilatation, mononuclear cell infiltration and periportal fibrosis. Renal sections in the same groups revealed interstitial hemorrhages, mononuclear cell infiltration, glomerulonephritis and proximal tubular necrosis. Hepato-renal histopathology was greatly reduced, particularly, in groups received combined antioxidants. The used antioxidants, therefore, exhibited potential protection against hepato-renal induced arsenic toxicity.

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