The Potential Role of Gymnema inodorum Leaf Extract Treatment in Hematological Parameters in Mice Infected with Plasmodium berghei

Malaria remains a significant cause of death in tropical and subtropical regions by serious complications with hematological abnormalities consistent with high parasitemia. Hence, this study aimed to determine the efficacy of the Gymnema inodorum leaf extract (GIE) on hematological alteration in Plasmodium berghei infection in mice. Groups of ICR mice were infected intraperitoneally with parasitized red blood cells of P. berghei ANKA (PbANKA). They were administered orally by gavage of 100, 250, and 500 mg/kg of GIE for 4 consecutive days. Healthy and untreated groups were given distilled water, while 10 mg/kg of chloroquine was treated as the positive control. Hematological parameters including RBC count, hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), RBC distribution width (RDW), white blood cell (WBC) count, and WBC differential count were measured. The results showed that significant decreases of RBC count, Hb, Hct, MCV, MCH, MCHC, and reticulocytes were observed in the untreated group, while RDW was significantly increased compared with the healthy control. Furthermore, the WBC, neutrophil, monocyte, basophil, and eosinophil of untreated mice increased significantly, while the lymphocyte was significantly decreased compared with the healthy control. Interestingly, GIE normalized the hematological alteration induced by PbANKA infection in GIE-treated groups compared with healthy and untreated groups. The highest efficacy of GIE was observed at a dose of 500 mg/kg. Our results confirmed that GIE presented the potential role in the treatment of hematological alteration during malaria infection.

Immunological and hematological effects of Irvingia gabonensis stem bark in sodium arsenite-exposed rats

This study investigated the effect of ethanol stem bark extract of Irvingia gabonensis (ESEIG) on sodium arsenite (SA)-induced pro-inflammatory cytokines and hematological perturbations in Wistar rats. Fifty-five Wistar rats weighing 100 g - 179 g were distributed into eleven groups (n=5). Group 1 had feed and water only. Group 2 received 4.1 mg/kg body weight (kgbw) of SA. Groups 3-11 received SA and/or ESEIG. Treatment was done orally for 14 days. Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), interleukin-4 (IL-4) concentrations, hemoglobin (HB) concentration, red blood cell (RBC) count, packed cell volume (PCV), mean cell hemoglobin concentration (MCHC), mean cell hemoglobin (MCH), mean corpuscular volume (MCV), white blood cell (WBC) count and its differentials and platelet (PLT) count were used to investigate the immunological and hematological effects of ESEIG. Exposure to SA produced significant (p ˂ 0.05) increases in hepatic IL-1β, TNF-α, IL-10 and IL-4 concentrations relative to control. Administration of SA also caused significant (p ˂ 0.05) decreases in HB, RBC, PCV, MCHC, MCH, MCV and PLT and significant (p ˂ 0.05) increases in WBC, lymphocytes, monocytes, eosinophils and neutrophils compared with control. Treatment with ESEIG concomitantly and 2 weeks after SA exposure, mitigated the deleterious effect of SA. However, ESEIG alone at various doses caused significant (p ˂ 0.05) increases in some of the assayed parameters, compared with control. These results imply that ESEIG may be protective against SA-induced inflammation and hematological derangements in Wistar rats. Its exclusive administration on chronic basis may also be slightly toxic.

Evaluation of Age Related Changes Associated with Exposure of Wistar Rats to Dichlorvor on some Haematological Parameters

Different studies proved that exposure over prolonged periods to pesticides increase the likelihood of developing adverse effects. 2,2-dichlorovinyl dimethyl phosphate (DDVP) is a predominant pesticide used in controlling insects. Therefore this study was aimed to investigate the age related changes in hematological indices of animals exposed to DDVP. Thirty healthy male Wistar rats were used in this study. Animals were grouped into three major groups of ten animals each. Group one animals served as young group (5 - 6 weeks) while group two and three was the middle-aged (2 months) and old groups (3-4 months) respectively. All the groups were subdivided into control and DDVP group with five animals in each subgroup. The DDVP groups were exposed to DDVP for five weeks. At the end of fifth week, they were euthanized with blood collected through cardiac puncture, hematological indices were analysed with autohematological analyzer.Exposure to DDVP significantly increased white blood cell counts and basophiles in old group. Platelets count and plateletcrit (PCT) were significantly decreased in young animals that were exposed to DDVP while platelet distribution width (PDW) and Mean platelets volume (MPV) were not significantly different across the groups. Red blood cells count, Hematocrit, Hemoglobin concentration, Mean cell volume, Mean cell hemoglobin and Mean cell hemoglobin concentration also show no significant difference across groups. Exposure of Wistar rats to Dichlorvos has no impact on haematological parameters in middle agerats. However, dichlorvos affects young and old animals proving age dependent effect of DDVP on hematological parameters. Keywords: DDVP, hematology, white blood cells, platelet, age

Hematological and Biochemical Parameters of Pregnant and Lactating Goats in Rangeland of Cholistan Desert, Bahawalpur, Pakistan

Jattal goats (n=90) of approximately 2-6 years of age being reared in Cholistan desert of Pakistan were studied during January to December, 2015 to examine the alterations in hematochemical parameters of Jattal goats at different reproductive phases while feeding on natural vegetations of desert areas of Cholistan. These were divided into three equal groups (non-pregnant, pregnant and lactating). Blood samples were collected by jugular vein puncture from goats of these three groups. Hematological parameters, white blood cells (WBC), lymphocytes (LYM), monocytes (MON), granulocytes (GRA), red blood cells ( RBC), hemoglobin (Hb), hematocrit ( HCT), mean corpuscular volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration ( MCHC) and red cell distribution width RDW were recorded. The results obtained revealed that all these parameters were generally lower in non-pregnant goats. The study revealed that significant higher values of hemoglobin (Hb), mean cell hemoglobin (MCH) and mean corpuscular volume (MCV) were observed in the lactating goats (P<0.05). Pregnant goats showed a significantly (P<0.05) higher values of leucocytes (WBC) than lactating and non-pregnant goats. Plasma sodium (Na) and potassium (K) concentration were markedly lower in lactating goats. Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly lower in pregnant goats. The Plasma concentration of cholesterol and triglycerides were higher in pregnant goats. In conclusion in present study changes in some hematochemical parameters have been determined in pregnant, non-pregnant and lactating goats of Jattal breed.

Suplementasi mikronutrien pada pasien eritema multiforme dengan penurunan kualitas eritrositMicronutrient supplementation in erythema multiforme patients with decreased erythrocyte quality

Pendahuluan: Eritema multiforme (EM) adalah penyakit peradangan akut yang merupakan reaksi hipersensitivitas pada mukokutan dan jarang ditemukan. Gambaran klinis EM berupa lesi erosi-ulseratif dan memiliki tendensi perdarahan. Kualitas eritrosit yang menurun dapat mempengaruhi proses penyembuhan penyakit ini, namun hal ini dapat diatasi dengan pemenuhan asupan mikronutrien. Tujuan laporan kasus ini untuk memaparkan hasil terapi suplementasi mikronutrien yang diberikan untuk tatalaksana EM pada pasien yang mengalami penurunan kualitas eritrosit. Laporan kasus: Seorang wanita berusia 21 tahun mengeluhkan perdarahan bibir dan sariawan dalam rongga mulut, terasa sakit sejak 3 minggu sebelumnya. Riwayat keluhan yang sama pernah dialami 7 tahun sebelumnya. Pemeriksaan ekstraoral memperlihatkan krusta hemoragik pada bibir, sedangkan hasil pemeriksaan intraoral menunjukkan ulserasi multipel pada hampir seluruh mukosa rongga mulut. Hasil pemeriksaan laboratorium menunjukkan adanya penurunan kualitas eritrosit berdasarkan parameter Mean Corpuscular Volume (MCV), Mean Cell Hemoglobin (MCH), dan Mean Cell Hemoglobin Concentration (MCHC); peningkatan jumlah eritrosit; dan eosinofil pada borderline atas, serta anti HSV-I IgG non-reaktif. Diagnosis pasien adalah eritema multiforme dengan riwayat stomatitis aftosa rekuren (SAR). Tatalaksana farmakologis diberikan asam folat dan vitamin B12 sebagai suplementasi mikronutrien. Diberikan pula chlorhexidine digluconate 0,12% mouthrinse sebagai antiseptik, dan petroleum jelly untuk pelembab bibir. Lesi oral pasien sembuh setelah 2 minggu terapi. Simpulan: Suplementasi mikronutrien berperan penting dalam mempercepat penyembuhan lesi mukosa oral pada pasien eritema multiforme yang mengalami penurunan kualitas eritrosit. Mikronutrien berupa asam folat dan vitamin B12 berperan penting dalam sintesis DNA eritrosit, pembelahan sel, dan perbaikan jaringan. Kata kunci: Mikronutrien, eritema multiforme, kualitas eritrosit. ABSTRACTIntroduction: Erythema multiforme (EM) is an acute inflammatory disease as a hypersensitivity reaction to mucocutaneous and is rarely found. The clinical feature of EM is an erosive-ulcerative lesion with a tendency of bleeding. Decreasing erythrocyte quality can affect its healing process, although able to be overcome by fulfilling the micronutrient intake. The purpose of this case report was to describe the results of micronutrient supplementation therapy given in the management of EM patients with decreasing erythrocyte quality. Case report: A 21-year-old woman complained of lip bleeding and oral thrush, which felt pain three weeks prior. A similar complaint history had been experienced in the previous seven years. Extraoral examination revealed haemorrhagic crusting on the lips, whereas intraoral examination showed multiple ulcerations of almost the entire oral mucosa. Laboratory examination results showed a decreasing erythrocyte quality based on the parameters of Mean Corpuscular Volume (MCV), Mean Cell Haemoglobin (MCH), and Mean Cell Haemoglobin Concentration (MCHC); an increasing number of erythrocytes and eosinophils in the upper borderline, as well as non-reactive anti-HSV-I IgG. The patient was diagnosed with erythema multiforme with a history of recurrent aphthous stomatitis (RAS). Pharmacological management was performed by given folic acid and vitamin B12 as micronutrient supplementation. Also, given a 0.12% chlorhexidine digluconate mouth rinse as an antiseptic and petroleum jelly for lip balm. The patient’s oral lesions resolved after two weeks of therapy. Conclusion: Micronutrient supplementation plays an essential role in accelerating the oral mucosal lesions healing in erythema multiforme patients with decreasing erythrocyte quality. Micronutrients in folic acid and vitamin B12 play an important role in erythrocyte DNA synthesis, cell division, and tissue repair.Keywords: Micronutrient, erythema multiforme, erythrocyte quality.

Does iron-fortified chewing gum influence the biochemical profile of school-going children (6–10 yrs.)?

Iron deficiency has become a common nutritional problem of developing countries, especially in children. This study approached to tackle the issue of iron deficiency by inexpensive fortified food such as chewing gums, which is commonly consumed by children. In this study, iron-fortified chewing gums were prepared by adding ferrous sulfate (FeSO4) and sodium iron EDTA (NaFeEDTA) 30 mg/100 g. An efficacy trial was conducted to determine the impact of iron-fortified chewing gums on the blood profile and iron status of school-going children (n = 300). Results showed maximum increase in blood profile and iron status that is, serum ferritin (10.43%), hemoglobin (3.22%), hematocrit (3.42%), red blood cells (3.05%), mean cell volume (1.55%), mean cell hemoglobin (5.43%), total white blood cells count (9.09%), and platelets count (4.40%) as compared with control whereas decrease in mean cell hemoglobin concentration (1.90%) and neutrophils (3.33%) was also observed. The study concluded that FeSO4 and NaFeEDTA (1:1) fortification of chewing gums is an appropriate approach for mitigating iron deficiency among the target population.

BLOOD MORPHOLOGICAL STATUS OF BLACK PIED SEED BULLSWHEN USING MINERAL SUPPLEMENT BASED ON MARAL ANTLER PRESS-CAKE

The usage period of seed-bulls, their sexual activity, the amount and quality of semen obtained from them depend both on the individual characteristics of the animal organ-ism and numerous environmental factors. The diet should contain all the necessary organic substances: easily digest-ible carbohydrates (starch and sugar), lipids, minerals and vitamins. The lack or deficiency of macro-and trace ele-ments in the diet and inadequate ratio of individual mineral elements disrupts the processes of nutrient intake into the animal body, thereby contributing to the disruption of meta-bolic processes. However, the mineral composition of plant forages depends largely on soil and climatic conditions, production and storage techniques. The average trace element deficiency in energy-balanced diets is 30-50%, and that necessitates using mineral supplements in animal diets. The nature of changes occurring in an animal body when feeding new forages, feed supplements, biological growth promoters and other products should and may be controlled through the main blood values. The research goal was to study the blood morphological composition of Black Pied seed bulls when using a mineral supplement based on maral antler press-cake. The experimental stud-ies were carried out in the breeding company AO “Plempredpriyatiye Barnaulskoye”, the Altai Region, in 2021, in the control and trial groups of comparable Black Pied seed bulls used on the farms of the Altai Region. Along with the basic diet, the seed bulls of the trial group received the mineral supplement based on maral antler press-cake; the daily dose -15.0 g per head, for 30 days with 10 days’ interval aftereach application. The supple-ment was mixed with compound feed before feeding. The mineral supplement was made at the Federal Altai Scien-tific Center of Agro-Biotechnologiesafter fermentation and autoclaving of maral antler press-cake; a significant amount of biologically active substances was found in ant-ler press-cake. The morphological status of the blood of seed bulls when using the mineral supplement made from maral antlers was characterized by a higher counts of erythrocytes, hemoglobin and leukocytes by 8.8%, 8.31% and 3.6%, higher values of hematocrit volume, mean cell hemoglobin (MCH), mean corpuscular volume (MCV), and mean cell hemoglobin concentration (MCHC) by 9.78%, 0.3%, 1.8% and 1.4%, respectively, as compared to those blood indices of the control group animals; that was indica-tive of its hematopoietic effect.

Effects of Justicia carnea Leave on Hematological Parameters in Albino Mice Carried Out in Mbingo Annex Hospital Laboratory in Bamenda, North West Region, Cameroon

This study is design to ascertain the hematological status in albino mice treated with Justicia carnea leave, which could aid in the treatment of anemia. The hematological parameters investigated include Mean Cell Volume (MCV), Mean Cell Hemoglobin (MCH), Mean Cell Hemoglobin Concentration (MCHC), Platelets (PLTS), Hemoglobin (Hb), Red Blood Cells (RBCs), Pack Cell Volume (PCV) and White Blood Cells (WBCs). Nine (9) male albino mice of approximately the same weight 300 g were grouped into 3 groups that’s Negative Control (NC) Positive Control (PC) and Test Control (TC), each made up of 3 mice. Negative control mice were given normal feed moisten with water, Positive control received diluted iron folate with normal feed, finally Test control mice were administered powdered leaves of Justicia carnea with normal feed for 7 days, after which blood samples were collected using EDTA tubes by exsanguination and run for hematological parameters using auto-hematological analyzer. The results of various groups were found to be; as there was a significant increase in RBCs, Hb, & PCV (P=0.05), between the groups. No significant change was observed on the MCV, MCH, and MCHC. Lymphocytosis was observed in all groups with mark difference in granulocytosis. TC had granulocytosis, NC showed normal granulocyte scores and the PC show granulocytopenia with a statistical difference of P=0.0017. A significant difference was seen in platelets between the groups P=0.02. This study shows that J. carnea leaves possess anti-anemic potential, lending credence to the use of these plant leaves in folk medicine for the management of hemolytic anemia would be helpful. Further research on the various phytochemicals of the plant should be done and also its toxicological aspects.

Profil Darah Ikan Gelodok (Periophthalmodon schlosseri) dan (Boleophthalamus boddarti) di Desa Kuala Tambangan Pelaihari, Kalimantan Selatan

Profil darah memiliki peran yang sangat penting dalam fisiologi metabolisme dan aktifitas tubuh hewan. Kuala Tambangan memiliki banyak potensi sumber daya ikan salah satunya ikan gelodok. Ikan gelodok di kawasan ini mudah ditemukan, akan tetapi sampai sekarang belum dimanfaatkan dengan baik oleh masyarakat. Tujuan penelitian ini untuk mengetahui profil darah ikan gelodok jenis Periophthalmodon schlosseri dan Boleophthalamus boddarti pada ekosistem mangrove yang berada di desa Kuala Tambangan, Kabupaten Tanah Laut, Kalimantan Selatan. Metode yang digunakan pada penelitian ini yaitu metode penangkapan hewan langka yaitu line transek, metode hemositometer dan metode sahli parameter yang dihitung hemoglobin, eritrosit, leukosit, hematokrit, MCV (Mean Corpusculla Volume), MCH (Mean Cell Hemoglobin), MCHC (Mean Cell Hemoglobin Concentration) pada 2 jenis ikan gelodok 34 ekor P. schlosseri dan 34 ekor B. boddarti. Hasil yang didapatkan adalah eritrosit P. schlosseri 3,87±0,58 x 106 sel/μL; B. boddarti 3,78±0,73 x 106 sel/μL 2 jenis ikan gelodok ini memiliki nilai eritrosit diatas batas normal; leukosit P. schlosseri 11,91±5,61 x103 sel/μL dan B. boddartii 9,72±4,24 x103 sel/μL nilai leukosit pada 2 jenis ikan gelodok ini berada di atas batas normal; hemoglobin P. schlosseri 11,59±1,75 % dan B. boddartii 11,75±1,96 % dari hasil yang didapat kadar hemoglobin pada 2 jenis ikan gelodok ini berada di atas batas normal kadar hemoglobin ikan pada umumnya; hematokrit P. schlosseri 34,32±5,57 % dan B. boddartii 35,71±5,44 % hasil hematokrit yang didapat dari 2 jenis ikan gelodok ini yaitu di atas batas normal; jenis P. schlosseri memiliki nilai MCV 88,72±6,62 μm3 yang berada di bawah batas normal; MCH 29,92±0,69 pg/sel yang berada di bawah batas normal; MCHC 33,99±3,97 g/dL pada jenis ini nilai MCHC masih berada pada batas normal; jenis B. boddartii memiliki nilai MCV 96,16±17,96 μm3 yang berada di bawah batas normal; MCH 31,51±5,50 pg/sel yang masih berada di batas normal; MCHC 32,87±1,77 g/dL nilai MCHC pada jenis ini masih berada pada batas normal. Blood profile has a very important role in the physiology of metabolism and animal body activities. Kuala Tambangan has a lot of potential fish resources, one of which is the fish Mudskipper. Mudskipper fish in this area are easy to find, but until now it has not been utilized properly by the community. The purpose of this study was to determine the blood profile of the Periophthalmodon Schlosseri and Boleophthalamus boddarti fish species in the mangrove ecosystem in the village of Kuala Tambangan, Tanah Laut District, South Kalimantan . The method used in this study is the method of catching endangered animals namely trasnek line, hemocytometer method and parameter Sahli method which is calculated hemoglobin, erythrocytes, leukocytes, hematocrit, MCV (Mean Corpuscular Volume), MCH (Mean Cell Hemoglobin), MCHC (Mean Cell Hemoglobin Concentration) on 2 types of fish, 34 Periophthalmodon schlosseri and 34 Boleophthalmus boddarti. The results obtained were P. schlosseri erythrocytes 3.87 ± 0.58 x 106 cells / μL; B. boddarti 3.78 ± 0.73 x 106 cells / μL 2 types of fish Mudskipper has erythrocyte values above the normal limit; P. schlosseri leukocytes 11.91 ± 5.61 x103 cells / μL and B. boddartii 9.72 ± 4.24 x103 cells / μL leukocyte values in these 2 types of jagged fish are above normal limits; hemoglobin P. schlosseri 11.59 ± 1.75 % and B. boddartii 11.75 ± 1.96 % from the results obtained by the hemoglobin levels in these 2 types of fishes are above the normal limit of fish hemoglobin levels in general; hematocrit P.schlosseri 34.32 ± 5.57 % and B. boddartii 35.71 ± 5.44 % hematocrit results obtained from these 2 types of mudskipper fish are above normal limits; P.schlosseri species had MCV values of 88.72 ± 6.62 μm3 which were below normal limits; MCH 29.92 ± 0.69 pg / cell that is below the normal range; MCHC 33.99 ± 3.97 g / dL in this type the MCHC value is still in the normal range; type B. boddartii has a MCV value of 96.16 ± 17.96 μm3 which is below normal limits; MCH 31,51 ± 5.50 pg / cell which is still in the normal range; MCHC 32.87 ± 1.77 g / dL MCHC values in this type are still within normal limits.Keywords: gelodok, blood, kuala tambangan

Hematological parameters in apparently Healthy Eritrean blood donors at the National Blood Transfusion Center, Asmara, Eritrea.

Introduction: This study was done to measure the Hemoglobin levels, Red Blood Cells count, and Red Blood cell parameters: MCV, MCH, MCHC, RDW, and Hematocrit of the apparently healthy blood donors attending the National Blood Bank of Eritrea. Methods: Blood samples were collected from the peripheral veins into the (EDTA)-tube and examined for Red Blood Cell count (RBC), hemoglobin (Hb), Hematocrit (%), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), and red cell distribution width (RDW), analyzed and measured by automated blood analyzer. Results: The mean Hemoglobin level was 14.428±1.485 g/dl, RBCs count was 4.744±0.482×1012/L, HCT was 41.929 ±3.75%, Red cell distribution width (RDW) mean was 13.571±0.744%, MCV was 88.582± 4.0558 Fimtoliter, MCH was 30.470±2.188 picogram, and MCHC was a mean of 34.393±1.347g/dl. The difference between males and females in MCV and MCHC was significant in favor of female donors. And a weak positive correlation had been found between the weight and age of donors and the Hemoglobin level. All the measured values were found to be within the global referenced ranges. Conclusion: Hemoglobin, RBCs count and RBCs indices of apparently healthy Eritrean blood donors were measured for reference, and all values were found within normal reference ranges.