Immunotherapy of Melanoma Targeting Human High Molecular Weight Melanoma-Associated Antigen: Potential Role of Nonimmunological Mechanisms

The primary motivation of developing ceramic materials using geopolymer method is to minimize the reliance on high sintering temperatures. The ultra-high molecular weight polyethylene (UHMWPE) was added as binder and reinforces the nepheline ceramics based geopolymer. The samples were sintered at 900 °C, 1000 °C, 1100 °C, and 1200 °C to elucidate the influence of sintering on the physical and microstructural properties. The results indicated that a maximum flexural strength of 92 MPa is attainable once the samples are used to be sintered at 1200 °C. It was also determined that the density, porosity, volumetric shrinkage, and water absorption of the samples also affected by the sintering due to the change of microstructure and crystallinity. The IR spectra reveal that the band at around 1400 cm−1 becomes weak, indicating that sodium carbonate decomposed and began to react with the silica and alumina released from gels to form nepheline phases. The sintering process influence in the development of the final microstructure thus improving the properties of the ceramic materials.

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Actinobacteria challenge the paradigm: A unique protein architecture for a well-known, central metabolic complex

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.2112107118 ◽

2021 ◽

Vol 118 (48) ◽

pp. e2112107118

Author(s):

Eduardo M. Bruch ◽

Pierre Vilela ◽

Lu Yang ◽

Alexandra Boyko ◽

Norik Lexa-Sapart ◽

...

Keyword(s):

Molecular Weight ◽

Metabolic Engineering ◽

High Molecular Weight ◽

Protein Oligomerization ◽

Central Metabolism ◽

Hollow Core ◽

Protein Architecture ◽

Unique Protein ◽

Gene Coding

α-oxoacid dehydrogenase complexes are large, tripartite enzymatic machineries carrying out key reactions in central metabolism. Extremely conserved across the tree of life, they have been, so far, all considered to be structured around a high–molecular weight hollow core, consisting of up to 60 subunits of the acyltransferase component. We provide here evidence that Actinobacteria break the rule by possessing an acetyltranferase component reduced to its minimally active, trimeric unit, characterized by a unique C-terminal helix bearing an actinobacterial specific insertion that precludes larger protein oligomerization. This particular feature, together with the presence of an odhA gene coding for both the decarboxylase and the acyltransferase domains on the same polypetide, is spread over Actinobacteria and reflects the association of PDH and ODH into a single physical complex. Considering the central role of the pyruvate and 2-oxoglutarate nodes in central metabolism, our findings pave the way to both therapeutic and metabolic engineering applications.

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Role of nano-fillers on tribological behaviour of ultra high molecular weight polyethylene composites

Materials Today Proceedings ◽

10.1016/j.matpr.2019.09.089 ◽

2020 ◽

Vol 27 ◽

pp. 2169-2173

Author(s):

B. Suresha ◽

B. Harshavardhan ◽

Ashwij M. Rao ◽

U.R. Koushik ◽

R. Hemanth

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Tribological Behaviour ◽

Ultra High Molecular Weight ◽

Polyethylene Composites

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Immunological Properties of a High Molecular Weight Component from Yeast Cell Autolysate in Dogs and Evaluation of Its Potential Role in Human Dextran Reactions

European Surgical Research ◽

10.1159/000127953 ◽

1977 ◽

Vol 9 (5) ◽

pp. 338-346 ◽

Cited By ~ 5

Author(s):

J. Ring ◽

H. Hedin ◽

W. Richter ◽

F. Jesch ◽

K. Messmer

Keyword(s):

Molecular Weight ◽

Yeast Cell ◽

High Molecular Weight ◽

Potential Role ◽

Immunological Properties ◽

High Molecular Weight Component

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Oligomerization and Dissociation of AP-1 Adaptors Are Regulated by Cargo Signals and by ArfGAP1-induced GTP Hydrolysis

Molecular Biology of the Cell ◽

10.1091/mbc.e05-06-0568 ◽

2005 ◽

Vol 16 (10) ◽

pp. 4745-4754 ◽

Cited By ~ 20

Author(s):

Daniel M. Meyer ◽

Pascal Crottet ◽

Bohumil Maco ◽

Elena Degtyar ◽

Dan Cassel ◽

...

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Adaptor Proteins ◽

Gtpase Activity ◽

Gtpase Activating Protein ◽

Gtp Hydrolysis ◽

Sorting Signals ◽

Initial Assembly ◽

Cargo Sorting

The mechanism of AP-1/clathrin coat formation was analyzed using purified adaptor proteins and synthetic liposomes presenting tyrosine sorting signals. AP-1 adaptors recruited in the presence of Arf1·GTP and sorting signals were found to oligomerize to high-molecular-weight complexes even in the absence of clathrin. The appendage domains of the AP-1 adaptins were not required for oligomerization. On GTP hydrolysis induced by the GTPase-activating protein ArfGAP1, the complexes were disassembled and AP-1 dissociated from the membrane. AP-1 stimulated ArfGAP1 activity, suggesting a role of AP-1 in the regulation of the Arf1 “GTPase timer.” In the presence of cytosol, AP-1 could be recruited to liposomes without sorting signals, consistent with the existence of docking factors in the cytosol. Under these conditions, however, AP-1 remained monomeric, and recruitment in the presence of GTP was short-lived. Sorting signals allowed stable recruitment and oligomerization also in the presence of cytosol. These results suggest a mechanism whereby initial assembly of AP-1 with Arf1·GTP and ArfGAP1 on the membrane stimulates Arf1 GTPase activity, whereas interaction with cargo induces oligomerization and reduces the rate of GTP hydrolysis, thus contributing to efficient cargo sorting.

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