Markers of melanoma progression in the blood serum structure.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e21035-e21035
Author(s):  
Alla I. Shikhlyarova ◽  
Oleg I. Kit ◽  
Olga N. Shatokhina ◽  
Amira A. Akhmedova ◽  
Yury Valentinovich Przhedetskiy ◽  
...  
Keyword(s):  
Solid State ◽  
Blood Serum ◽  
Prognostic Markers ◽  
Treatment Effectiveness ◽  
Polarized Light ◽  
Cover Glass ◽  
Early Appearance ◽  
M Proteins ◽  
Cell Dehydration ◽  
Extracellular Environment

e21035 Background: Lack of prognostic markers of melanoma to assess its progression, metastasis (MTS), and treatment effectiveness, determines the feasibility of studying biophysical aspects of the transmission of events from cellular to extracellular environment. Receipt and circulation of pathological proteins of melanoma (M) in the blood carries important information, and its trap is the phase transition of biofluids into a solid state. The purpose of the study was to determine markers of melanoma at the supramolecular level in solid-state samples of the blood serum (BS). Methods: 240 samples of BS obtained prior to the surgery from 60 patients with M were studied. The preparations were prepared by the Shatokhina-Shabalin method by creating a closed "analytic cell": dehydration of a BS drop (10 mcL) under a cover glass at 20–240С, humidity 65–70%, for 72 hours. The folding of M proteins was assessed by a polarized light microscopy (Leica DM LS2 microscope, x400-640) using photo and video control and the Morphotest program. Results: The initial stages of M development were characterized by the early appearance of single combined melanoform crystals — anisotropic microspherolites (Mi) embedded in the center of a large anisomorphone (lAM). As M progressed, Mi aggregation with basic lAMs was enhanced. Anisotropy of Mi increased, revealing clear sectors filled with melanin. In the later stages of M growth, melanoform aggregations dominated with point, filamentous, and chained melanin structures. The manifestation of MTS was accompanied by the destruction of the aggregations and the filling of the analytic cell space with numerous Mi. Conclusions: Correspondence of crystallogenesis of M melanomorphic markers in BS in tumor progression allows using the studied supramolecular factors to predict the development of melanoma.

Microscopy in Pharmaceutical Development

1998 ◽  
Vol 4 (S2) ◽  
pp. 478-479
Author(s):  
R. A. Carlton
Keyword(s):  
Solid State ◽  
Polarized Light ◽  
Particle Size Analysis ◽  
Primary Objective ◽  
Drug Substance ◽  
Size Analysis ◽  
Pharmaceutical Development ◽  
Drug Candidates ◽  
Infrared Microspectroscopy ◽  
The Face

Many different microscopic techniques are utilized in the development of new pharmaceuticals. For that reason, pharmaceutical companies have supported microscopy and microscopists even in the face of retrenchments in many other industries. Microscopy has the following three major uses in pharmaceutical development: 1) solid-state characterization of drug substance; 2) particle size analysis; 3) contaminant identification. Microscopy is also an important component of the study of the biological activity of drugs but that subject will not be discussed here.The analysis of the solid-state properties of new drug candidates is probably the most important and challenging subject of study for pharmaceutical microscopists.1 The primary objective of these studies is identification of the most suitable solid-state form (i.e. polymorph, solvate, hydrate) of the drug substance for development. Polarized light microscopy (PLM), thermal microscopy (TM), infrared microspectroscopy (IR), and Raman microspectroscopy (RM) are all utilized in these studies. Both PLM and TM are used to discover new polymorphs and solvates of the chemical.

scholarly journals Optical anticounterfeiting photonic bilayer film based on handedness of solid-state helicoidal structure

RSC Advances ◽  
2021 ◽  
Vol 11 (59) ◽  
pp. 37498-37503
Author(s):  
Saddam Hussain ◽  
Sajjad Haider ◽  
Waheed Al-Masry ◽  
Soo-Young Park
Keyword(s):  
Solid State ◽  
Polarized Light ◽  
Bilayer Film ◽  
Helicoidal Structure ◽  
Circular Polarized Light

Anticounterfeiting photonic bilayer DH-CLCsolid films were fabricated by sandwiching two CLCsolid films having different handedness. The encrypted information is only disclosed under right-handed circular polarized light.

The Role of Microscopy in the Study of Chemical Polymorphism

2000 ◽  
Vol 6 (S2) ◽  
pp. 1000-1001
Author(s):  
R.A. Carlton
Keyword(s):  
Solid State ◽  
Development Process ◽  
Polarized Light ◽  
Crystal Form ◽  
Chemical Polymorphism ◽  
Optic Angle ◽  
Temperature And Pressure ◽  
Metastable Forms ◽  
The Relationship

Chemical polymorphism refers to the ability of compounds to exist in different solid state forms. In general, these differ solely in their arrangement in space (i.e., their crystal form). Polymorphs typically have different solid state properties such as melting point, solubility, density, etc. They also commonly exhibit distinctive optical properties such as refractive index, extinction position, optic sign and optic angle. The choice of polymorph can be an important consideration in the drug development process if the solid state properties have wide differences. Further, it is generally desirable to develop the polymorph which is stable at room temperature and pressure, since metastable forms convert spontaneously to the stable ones, albeit often at a very slow rate. Optical, thermal and scanning electron microscopy each play important roles in the study of chemical polymorphism.Optical microscopy, and more specifically polarized light microscopy, contributes both to the discovery of new polymorphs and to the elucidation of the relationship among polymorphs.

Water dispersed fluorescent organic aggregates for the picomolar detection of ClO4−in water, soil and blood serum and the attogram detection of ClO4−in the solid state by a contact mode method

2016 ◽  
Vol 4 (31) ◽  
pp. 7420-7429 ◽  
Author(s):  
Rahul Kumar ◽  
Sana Sandhu ◽  
Prabhpreet Singh ◽  
Subodh Kumar
Keyword(s):  
Solid State ◽  
Blood Serum ◽  
Inorganic Anions ◽  
Molecular Probe ◽  
Contact Mode ◽  
Mode Method

An aggregation–disaggregation based molecular probe allows the detection of 10 pM ClO4−in solution and 6 × 10−18g cm−2ClO4−in the solid state by a contact mode method with a selectivity of ∼10 000 over other inorganic anions.

scholarly journals II. On the anatomy of the connective tissues

1875 ◽  
Vol 23 (156-163) ◽  
pp. 184-187
Keyword(s):  
Blood Serum ◽  
Aqueous Humour ◽  
Animal Tissues ◽  
Connective Tissues ◽  
Cover Glass ◽  
Horizontal Section

Transparent animal tissues, when sealed up fresh in aqueous humour or blood-serum, by running Brunswick black round the edge of the cover glass, undergo a series of slow changes, by which, generally within a period of 2 to 5 days, anatomical elements mostly otherwise invisible become distinct. The paper is chiefly a record of observations made by this method. The author shows:— After a horizontal section of the cornea has been sealed up for about 2 hours, the stellate branched cells are seen to consist of a mass of pro-tlasma, sharply defined on every side, except where it is continued for scarcely perceptible distance into the processes. The nucleus is flatted.

A Marker of Cerebral Ischemia in Solid State Structures of Blood Serum

2018 ◽  
Vol 164 (3) ◽  
pp. 366-370 ◽  
Author(s):  
S. N. Shatokhina ◽  
V. V. Aleksandrin ◽  
I. S. Shatokhina ◽  
A. A. Kubatiev ◽  
V. N. Shabalin
Keyword(s):  
Solid State ◽  
Cerebral Ischemia ◽  
Blood Serum ◽  
Solid State Structures

scholarly journals SYNTHESIS AND PROPERTIES OF NANOCELLULOSE-DYE CONJUGATES

2021 ◽  
Vol 64 (2) ◽  
pp. 10-18
Author(s):  
Oleg V. Surov ◽  
Marina I. Voronova ◽  
Natalia V. Rubleva ◽  
Anatoly G. Zakharov ◽  
Andrei V. Afineevskii
Keyword(s):  
Solid State ◽  
Elemental Analysis ◽  
Colloidal Stability ◽  
Polarized Light ◽  
Degree Of Crystallinity ◽  
Elemental Analysis Data ◽  
Absorption Bands ◽  
Aqueous Suspensions ◽  
Vis Spectroscopy ◽  
Scanning Electron

Synthesis of conjugates of cellulose nanocrystals (CNC) with chlortriazine reactive dyes (RD) has been developed.  The obtained CNC-RD conjugates were characterized using a complex of modern methods: UV-Vis, IR and 13C solid state NMR spectroscopy, thermogravimetric and elemental analysis, particle size and zeta potential analysis, scanning electron and polarization optical microscopy. IR spectra of CNC-RD films show absorption bands typical of cellulose. The formation of a covalent cellulose-dye bond is confirmed by the presence of characteristic absorption bands of the resulting ether bond in the region of 1000-1300 cm-1. Based on the analysis of solid-state 13C NMR spectra, it was concluded that the degree of crystallinity of the CNC-RD conjugates does not change compared to the CNC, i.e. the RD covalently bind to the surface of the CNC particles and do not affect the crystal structure of the CNC. Using a simple model of CNC with square cross-section, and based on the elemental analysis data, the amount of covalently bound dye on the surface of CNC particles was estimated. The results obtained allow us to conclude that covalent bound of RD occurs selectively at one of the ends of the cellulose nanocrystal (the so-called reducing end of the cellulose polymer chain). Successful CNC surface modification is confirmed by UV-Vis spectroscopy. It was found that aqueous suspensions of CNC-RD conjugates have indicator properties. The sizes and charges of the particles of CNC-RD conjugates in dilute aqueous suspensions were determined. High colloidal stability of the CNC-RD aqueous suspensions was confirmed. It has been shown that CNC-RD colloidal suspensions possess liquid crystalline properties, which is clearly demonstrated by their iridescent color in polarized light with stirring. CNC films modified with RD also possess birefringence properties and exhibit an iridescent color in polarized light. The morphology of CNC-RD particles was studied using scanning electron microscopy. The thermal stability of the CNC-RD conjugates was studied.

scholarly journals Rediscovery of Darkfieid Dispersion Staining while Building a Universal Student Microscope

2003 ◽  
Vol 11 (1) ◽  
pp. 24-28
Author(s):  
Theodore M. Clarke
Keyword(s):  
Light Source ◽  
Light Microscope ◽  
Fiber Optic ◽  
Light Guide ◽  
Polarized Light ◽  
Cover Glass ◽  
Artistic Value ◽  
Aperture Diaphragm ◽  
Reflected Light

My first universal student microscope, shown in Figure 1, began life as a Monolux microscope from the 1960rs. Its development into a universal student microscope began when my wife wanted a polarized light microscope with the ability to photograph microscopic crystals under a cover glass for their artistic value. My background as a metallurgist was with the reflected light metallurgical microscope, I have also designed and built vertical illuminators for brightfield illumination of complete metal log raphic specimens using a fiber optic light guide end as the light source with lens configurations giving an imaged field diaphragm and an illumination aperture diaphragm imaged in the aperture of the macro lens.

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