Adult ovarian granulosa cell tumor: Role of systemic chemotherapy and surgical treatment.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e18083-e18083
Author(s):  
Anna Beliaeva ◽  
Elena Bakhidze
Keyword(s):  
Surgical Treatment ◽  
Adjuvant Chemotherapy ◽  
Drug Treatment ◽  
Granulosa Cell ◽  
Critical Role ◽  
Granulosa Cell Tumor ◽  
Cell Tumor ◽  
Low Grade ◽  
Significant Difference

e18083 Background: Granulosa cell tumor (GCT) are rare sex cord-stromal tumours. Their frequency is approximately 3% - 5% of all malignant ovarian tumors, and the incidence is from 0.6 to 2.1 cases per 100 000 women per year. According to the histological features, GCT are divided into two types: adult and juvenile. In the 2014 WHO histological classification, GCT were divided into borderline, which included the GCT of the juvenile type, and the malignant GCT of the adult type (AGCT). Despite the definition of AGCT not as borderline, but as low-grade malignant tumors, there are no specific recommendations for the surgical and drug treatment in such patients. Main method of treatment is surgical. Role of systemic treatment due to rarity of AGCT and late recurrence still has not determined. Methods: Data of 93 patients in our institution who were diagnosed with AGCT I-IV stage of the disease between 1980 and 2017 were evaluated. The data were obtained from the files of the patients, electronic database of the gynecologic oncology clinic, operation notes, and pathology records. Results: Stage of the disease, the spread of the tumor beyond the capsule and the number of mitoses of more than 10 significantly influenced the OS rates (p < 0.05). The average time before the onset of the first relapse in IA, IB stage was 134.5 months, in IC stage - 67.4 months, in II-IV stage - 34.3 months. Stage of the disease is an independent prognostic factor for the occurrence of a relapse of the disease. In stage I disease there was no influence of the volume of surgical treatment and adjuvant chemotherapy on DFS and the duration of disease-free period (p > 0.05). The duration of the non-progressive period was some, but not significantly more when performing optimal cytoreduction for the recurrence of the disease, and did not depend on the implementation regimen and adjuvant chemotherapy. Conclusions: There were not found significant difference in the overall and relapse-free survival of patients with ovarian AGCT, depending on the options of surgical and drug treatment they underwent. Studies showed that hormones play a critical role in the pathogenesis and treatment of GCT, especially in some ineffective cases for radiotherapy and chemotherapy. Additional multicenter randomized trials are needed to clarify the effectiveness of the various options for surgical and drug treatment of adult GCT patients.

scholarly journals FOXO1 mitigates the SMAD3/FOXL2C134W transcriptomic effect in a model of human adult granulosa cell tumor

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Christian Secchi ◽  
Paola Benaglio ◽  
Francesca Mulas ◽  
Martina Belli ◽  
Dwayne Stupack ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Chromatin Remodeling ◽  
Granulosa Cell Tumor ◽  
Cell Tumor ◽  
Rna Seq ◽  
Pathogenic Role ◽  
Rare Type ◽  
Cellular Models ◽  
Genome Wide

Abstract Background Adult granulosa cell tumor (aGCT) is a rare type of stromal cell malignant cancer of the ovary characterized by elevated estrogen levels. aGCTs ubiquitously harbor a somatic mutation in FOXL2 gene, Cys134Trp (c.402C < G); however, the general molecular effect of this mutation and its putative pathogenic role in aGCT tumorigenesis is not completely understood. We previously studied the role of FOXL2C134W, its partner SMAD3 and its antagonist FOXO1 in cellular models of aGCT. Methods In this work, seeking more comprehensive profiling of FOXL2C134W transcriptomic effects, we performed an RNA-seq analysis comparing the effect of FOXL2WT/SMAD3 and FOXL2C134W/SMAD3 overexpression in an established human GC line (HGrC1), which is not luteinized, and bears normal alleles of FOXL2. Results Our data shows that FOXL2C134W/SMAD3 overexpression alters the expression of 717 genes. These genes include known and novel FOXL2 targets (TGFB2, SMARCA4, HSPG2, MKI67, NFKBIA) and are enriched for neoplastic pathways (Proteoglycans in Cancer, Chromatin remodeling, Apoptosis, Tissue Morphogenesis, Tyrosine Kinase Receptors). We additionally expressed the FOXL2 antagonistic Forkhead protein, FOXO1. Surprisingly, overexpression of FOXO1 mitigated 40% of the altered genome-wide effects specifically related to FOXL2C134W, suggesting it can be a new target for aGCT treatment. Conclusions Our transcriptomic data provide novel insights into potential genes (FOXO1 regulated) that could be used as biomarkers of efficacy in aGCT patients.

Ovarian granulosa cell tumor: A Clinicoradiologic series with literature review

2020 ◽  
Vol 16 ◽  
Author(s):  
Yagmur Kilinc ◽  
Lutfullah Sari ◽  
Huseyin Toprak ◽  
Mehmet Gultekin ◽  
Ummuhan Karabulut ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Wide Spectrum ◽  
Case Series ◽  
Granulosa Cell Tumor ◽  
Cell Tumor ◽  
University Hospital ◽  
Low Grade ◽  
Granulosa Cell Tumors ◽  
Mri Examination ◽  
Ovarian Granulosa Cell

Background: Ovarian granulosa cell tumors that originate from the sex cord-stromal cells represent 2% to 5% of all ovarian cancers. These tumors constitute two subgroups according to their clinical and histopathological features: juvenile granulosa cell tumors (JGCT) and adult granulosa cell tumors (AGCT). Granulosa cell tumor (GCT) is considered to be a low-grade malignancy with a favorable prognosis. Methods: This case series includes four patients who admitted to our university hospital and had an MRI examination within 5 years. Results: The histopathological subtype of granulosa tumor was the adult type in 3 patients and juvenile type in 1 patient. Even though its extremely rare, bone metastases were present in one of our patients. Liver metastases were also detected in one patient. The MRI examination of tumors revealed a heterogeneous solid mass that contained cystic components in 3 patients. In one of our patients, the tumor had a multiseptated cystic feature, and all of the tumors were ovoid or round with smooth margins. T1 signal hyperintensity, not suppressed on fat saturation sequences, observed in our 3 patients, which represents its hemorrhagic content. Conclusion: Even though granulosa cell tumor shows a wide spectrum in terms of tumor appearance, some common findings have been shown and especially a hemorrhagic content could be a clue for us. The tumor is known to have a good prognosis, but it may have an unpredictable clinical course, so close follow-up has a great importance.

scholarly journals Role of Anti-Müllerian Hormone in Diagnosing Granulosa Cell Tumor: A Case Report

2012 ◽  
Vol 5 (2) ◽  
pp. 94-96
Author(s):  
Aarti Umranikar ◽  
Mili Saran ◽  
Nick Brook ◽  
Neeta Singh ◽  
Darren Fowler ◽  
...  
Keyword(s):  
Case Report ◽  
Granulosa Cell ◽  
Granulosa Cell Tumor ◽  
Cell Tumor

scholarly journals A very rare case of adult-type granulosa cell tumor

2015 ◽  
Vol 87 (1) ◽  
pp. 98 ◽  
Author(s):  
Roberto Giulianelli ◽  
Gabriella Mirabile ◽  
Giorgio Vincenti ◽  
Francesco Pellegrino ◽  
Giuseppe Soda
Keyword(s):  
Surgical Treatment ◽  
Granulosa Cell ◽  
Rare Case ◽  
Granulosa Cell Tumor ◽  
Cell Tumor ◽  
Adult Type ◽  
Rare Neoplasm

Granulosa cell tumor (GST) of the testis is a rare neoplasm. Here we describe a case of an adult type GST. More than a year after surgical treatment, without any other treatment, the patient is alive without sign of disease.

scholarly journals Is There a Way to Predict Granulosa Cell Tumor of the Ovary? The Role of Peripheral Blood Test Parameters

2020 ◽  
Author(s):  
Muzaffer Seyhan Cikman ◽  
İsmet Gün ◽  
Önder Sakin ◽  
Kazibe Koyuncu ◽  
Ali Doğukan Anğın ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Peripheral Blood ◽  
Blood Test ◽  
Granulosa Cell Tumor ◽  
Cell Tumor ◽  
Test Parameters

scholarly journals LncRNA gas5 regulates granulosa cell apoptosis and viability following radiation by x-ray via sponging miR-205- 5p and Wnt/β-catenin signaling pathway in granulosa cell tumor of ovary

2020 ◽  
Vol 19 (6) ◽  
pp. 1153-1159
Author(s):  
Yan Li ◽  
Xing Ma ◽  
Jun Li ◽  
Saifei He ◽  
Juhua Zhuang ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Signaling Pathway ◽  
Granulosa Cell ◽  
Granulosa Cell Tumor ◽  
Cell Tumor ◽  
X Ray ◽  
Qrt Pcr ◽  
Lncrna Gas5

Purpose: To investigate the role of lncRNA gas5 in ovarian granulosa cells exposed to x-ray in granulosa cell tumor of ovary (GCTO). Methods: KGN cell line was exposed to X-ray to mimic the radiotherapy for GCSO patients in vitro, cell viability was checked by CCK8 assays. RNA expression of apoptosis-related genes was determined by quantitative reverse transcriptase-polymerase reaction (qRT-PCR) while Western Blot for biomarkers in wnt/β-catenin signaling. Differential expressions of lncRNA gas5 were examined after cells were exposed to a ray for 0,24,48hs. We over expressed gas5 and assessed resultant cell viability, apoptosis and signaling. The sponging between gas5 and miR-205-5p was verified by luciferase assay. CCK8, qRT-PCR and Western blot were applied to investigate the correlation between miR-205-5p, cell viability, and apoptosis after miR-205-5p augmentation. Similarly, interaction between gas5 and miR-205-5p was assessed after co-transfection of miR-205-5p mimics and oe-gas5. Finally, wnt inhibitor was used to study the role of signaling pathway in KGN cells. Results: Exposure of KGN to x-ray reduced cell viability and increased apoptosis. Gas5showed reduced expression in the cells, while miR-205-5p  expression increased. Gas5 upregulation protected the cells against apoptosis and contributed to cell viability and activation of wnt//β-catenin signaling. lncRNA gas5 targeted miR-205-5p and miR-205-5p mimics counteracted the functions of up-regulated lncRNA gas5, regulating Wnt/β-catenin signaling pathway. Inactivation of Wnt/β-catenin suppressed cell viability. Conclusions: lncRNA gas5 regulates cell apoptosis and viability following cellular radiation, thus presenting a potential therapeutic target for the application radiotherapy in GCTO patients. Keywords: Ovary, Proliferation, Apoptosis, lncRNA gas5, Radiotherapy, β-catenin signaling

scholarly journals Is adjuvant chemotherapy beneficial for patients with FIGO stage IC adult granulosa cell tumor of the ovary?

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Dan Wang ◽  
Yang Xiang ◽  
Ming Wu ◽  
Keng Shen ◽  
Jiaxin Yang ◽  
...  
Keyword(s):  
Adjuvant Chemotherapy ◽  
Granulosa Cell ◽  
Granulosa Cell Tumor ◽  
Figo Stage ◽  
Cell Tumor

scholarly journals Three Different Ways to Miss a Granulosa Cell Tumor – A Lesson to be Learne

2021 ◽  
Vol 6 (3) ◽  
pp. 345-348
Author(s):  
Neethu Puthalon Kunnath ◽  
Sony Nanda ◽  
Janmejaya Mohapatra
Keyword(s):  
Endometrial Carcinoma ◽  
Granulosa Cell ◽  
Frozen Section ◽  
Granulosa Cell Tumor ◽  
Total Abdominal Hysterectomy ◽  
Late Recurrence ◽  
Cell Tumor ◽  
Low Grade ◽  
Biological Behaviour ◽  
Operative Recovery

Granulosa cell tumours (GCTs) account for approximately 70% of malignant sex-cord stromal tumors but are still uncommon and comprise only 2–5% of all ovarian neoplasms. They are classified as adult and juvenile GCTs. These are often low grade malignancies and are usually diagnosed in early stages, but with an potential for late recurrence. Case 1: A 51-year woman with c/o PMB with biopsy showing endometrial polyp. She underwent a total abdominal hysterectomy and a bilateral salpingo-oophorectomy (TAH BSO) with omental biopsy after intraoperative pathology confirmation of a Adult granulosa cell tumor (AGCT) of the ovary. She had a good post-operative recovery and was advised for regular and long term follow up. Case 2: A 63-year woman c/o PMB with an adult granulosa cell tumor that initially presented as endometrial hyperplasia on biopsy. She underwent a TAH BSO and omental biopsy after intraop frozen section confirmed of AGCT of the ovary. She had an uneventful post-operative recovery. Case 3: A 68-year womanwith an AGCT that was initially treated as endometrial carcinoma. She underwent a Comprehensive Surgical Staging due to initial misdiagnosis of endometrial carcinoma. Her final biopsy report showed it to be a granulosa cell tumor of the ovary and adenocarcinoma of the endometrium. She had a good post-operative recovery and is being followed up till now. The multifaceted presentations with its erratic biological behaviour coupled with late recurrences are diagnostic pitfalls necessitating a high degree of suspicion for accurate clinical diagnosis.

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