Nano-hydroxyapatite/chitosan-chondroitin sulfate composites materials with different weight ratios were prepared through liquid co-precipitation method. The nano-Hap、Hap/Cs and Hap/Cs-Chs composites materials under the same conditions preparation materials were researched By XRD, SEM and EDS respectively. The results show that nano-Hap particles with poorly crystallinity, the size is about 20nm, nano-Hap particles are spherical in sHape and dispersed uniformly, combined with relatively loose between particles, the small nano-particles aggregate into larger particles when the Hap composite with the Cs and Chs, nano-Hap were dispersed uniformly in the organic phase Cs and Chs, Hap particles have the trend to grow large in composites materials,crystal become more perfect, combination become relatively dense between particles, The composites materials were annealed at 700°C,Hap crystal particles become more perfect clear and larger with relatively loose between particles.EDS analysis showed that the compound before and after annealing of the material ratio of calcium had no influence, material does not contain any impurities,The ratio of calcium and phosphorus is about 1.75:1,Hap belonging to the type of calcium-rich
