Chlorophyll fluorescence as a tool for describing the operation and regulation of photosynthesis in vivo

2018 ◽  
pp. 539-571
Keyword(s):  
Chlorophyll Fluorescence ◽  
Regulation Of Photosynthesis

In Vivo Fluorescence of Chlorophyll A: Estimation of Phytoplankton Biomass and Activity in Římov Reservoir (Czech Republic)

1993 ◽  
Vol 28 (6) ◽  
pp. 29-33 ◽  
Author(s):  
V. Vyhnálek ◽  
Z. Fišar ◽  
A. Fišarová ◽  
J. Komárková
Keyword(s):  
Czech Republic ◽  
Chlorophyll Fluorescence ◽  
Primary Production ◽  
Chlorophyll A ◽  
Phytoplankton Biomass ◽  
In Vivo Fluorescence ◽  
Fluorescence Of Chlorophyll A ◽  
Římov Reservoir ◽  
Natural Phytoplankton

The in vivo fluorescence of chlorophyll a was measured in samples of natural phytoplankton taken from the Římov Reservoir (Czech Republic) during the years 1987 and 1988. The fluorescence intensities of samples either with or without addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron, DCMU) were found reliable for calculating the concentration of chlorophyll a during periods when cyanobacteria were not abundant. The correction for background non-chlorophyll fluorescence appeared to be essential. No distinct correlation between a DCMU-induced increase of the fluorescence and primary production of phytoplankton was found.

Drought resistance of hard wheat cultivars measured by a rapid chlorophyll fluorescence test

1985 ◽  
Vol 104 (3) ◽  
pp. 501-504 ◽  
Author(s):  
M. Havaux ◽  
R. Lannoye
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Induction ◽  
Chlorophyll Fluorescence Induction ◽  
Quenching Rate ◽  
Simple Method ◽  
Hard Wheat ◽  
Drought Tolerant ◽  
Resistant Varieties ◽  
Slow Fluorescence Induction

SummaryDisks of hard wheat (Triticum durum Desf.) leaves subjected to rapid desiccation over 4 h showed noticeable changes in the shape of the in vivo chlorophyll fluorescence induction curves. In drought-sensitive varieties (such as Claridoc), water stress resulted in a strong inhibition of the slow fluorescence induction transients. In particular, the fluorescence quenching rate was markedly decreased in water-stressed leaf disks. In contrast, leaves of drought-resistant varieties (such as Aouedj) showed only minor changes in chlorophyll fluorescence. The results of this investigation suggest that the slow transient of the in vivo chlorophyll fluorescence induction phenomenon may provide a simple method for selecting drought-tolerant wheats.

scholarly journals An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5589
Author(s):  
João Serôdio ◽  
William Schmidt ◽  
Jörg C. Frommlet ◽  
Gregor Christa ◽  
Matthew R. Nitschke
Keyword(s):  
Chlorophyll Fluorescence ◽  
High Throughput ◽  
Light Stress ◽  
Limiting Factor ◽  
Phenotypic Traits ◽  
Light Responses ◽  
Illumination System ◽  
Digital Manipulation ◽  
Multiple Samples

The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosynthetic productivity in algae and plants. The quantitative characterization of light responses is often time-consuming and calls for cost-effective high throughput approaches that enable the fast screening of multiple samples. Here we present a novel illumination system based on the concept of ‘multi-actinic imaging’ of in vivo chlorophyll fluorescence. The system is based on the combination of an array of individually addressable low power RGBW LEDs and custom-designed well plates, allowing for the independent illumination of 64 samples through the digital manipulation of both exposure duration and light intensity. The illumination system is inexpensive and easily fabricated, based on open source electronics, off-the-shelf components, and 3D-printed parts, and is optimized for imaging of chlorophyll fluorescence. The high-throughput potential of the system is illustrated by assessing the functional diversity in light responses of marine macroalgal species, through the fast and simultaneous determination of kinetic parameters characterizing the response to light stress of multiple samples. Although the presented illumination system was primarily designed for the measurement of phenotypic traits related to photosynthetic activity and photoinhibition, it can be potentially used for a number of alternative applications, including the measurement of chloroplast phototaxis and action spectra, or as the basis for microphotobioreactors.

scholarly journals Impact of Exposure to Low Temperature Degrees in Field Conditions on Leaf Pigments and Chlorophyll Fluorescence in Leaves of Mango Trees

2020 ◽  
Vol 10 ◽  
pp. 30-45
Author(s):  
Ali A.S. Sayed ◽  
Farouk M. Gadallah ◽  
Mohamed A. Seif El-Yazal ◽  
Gamal A. Abdel-Samad
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Chlorophyll Concentration ◽  
Ambient Air ◽  
Sampling Time ◽  
Chlorophyll B ◽  
Physiological And Biochemical ◽  
Sampling Times ◽  
Fayoum Governorate

This experiment was conducted to found the connection between low temperature stress in vivo conditions (ambient-air temperature) and the changes in some physiological and biochemical events (leaf pigments and chlorophyll fluorescence) of mango trees in response to exposure to natural low temperature (cold). To verify this objective, 12 popular commonly mango cultivars (25 years old) which grown in private orchard in Fayoum Governorate, Egypt were selected for this study which carried out during the period from November to March of years; 2012 and 2013. The selected cultivars were: Alphonso, Baladi, Bullock's Heart, Helmand, Hindi Besennara, Mabrouka, Mestekawy, Nabeeh, Oweisi, Spates, Taimour and Zebda. Based on the obtained results, it can be stated that, chlorophyll (a) concentration in the leaves was significantly differed among the cultivars throughout the whole sampling times, in this respect, Helmand one gave the highest one while, and the highest one by sampling times was November one. The concentration of chlorophyll (b) was significant as effected by the effect of cultivars and sampling time recorded the highest value by the cultivar of Spates and December sample, respectively. Total chlorophyll concentration in the leaves reached its peak by the cultivar of Nabeeh and sampling time of December as compared to others. The both of Ewais cultivar and the sample of March showed the highest values of carotenoids concentration in the leaves. The levels of anthocyanin in leaves were significantly differed as affected by the cultivars and sampling times, indicating that the cultivar of Helmand and November sample recorded the highest values of anthocyanin in leaves. The greatest reductions in Fv/Fmratio were recorded at month of November and indicated that the reductions were in the order of Alphonso˃ Mabrouka˃Taimour˃ others. The effect of sampling time, cultivars and their interaction on Fv/Fm were significant, but small between some values of Fv/Fm.

The Role of Orthophosphate in the Regulation of Photosynthesis in vivo

1987 ◽  
pp. 325-328 ◽  
Author(s):  
I. Madhusudana Rao ◽  
Javier Abadia ◽  
Norman Terry
Keyword(s):  
Regulation Of Photosynthesis
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