Investigating Human Skin Barrier Lipids with In Vitro Skin Models

2001 ◽  
pp. 121-147
Author(s):  
Annie Black ◽  
Odile Damour ◽  
Kordula Schlotmann
Keyword(s):  
Human Skin ◽  
Skin Barrier

Electroperturbation of the human skin barrier in vitro (I): the influence of current density on the thermal behaviour of skin impedance

1997 ◽  
Vol 43 (1) ◽  
pp. 43-50 ◽  
Author(s):  
W.H.M. Craane-van Hinsberg ◽  
J.C. Verhoef ◽  
H.E. Junginger ◽  
H.E. Boddé
Keyword(s):  
Human Skin ◽  
Thermal Behaviour ◽  
Current Density ◽  
Skin Barrier ◽  
Skin Impedance

In vitro human skin barrier perturbation by oleic acid: Thermal analysis and freeze fracture electron microscopy studies

1997 ◽  
Vol 293 (1-2) ◽  
pp. 77-85 ◽  
Author(s):  
Hanafi Tanojo ◽  
Anita Bos-van Geest ◽  
Joke A. Bouwstra ◽  
Hans E. Junginger ◽  
Harry E. Boodé
Keyword(s):  
Electron Microscopy ◽  
Thermal Analysis ◽  
Oleic Acid ◽  
Human Skin ◽  
Freeze Fracture ◽  
Skin Barrier ◽  
Freeze Fracture Electron Microscopy ◽  
Fracture Electron

scholarly journals Niosome Encapsulated Bromelain Reduced IL-6 and TNF-α in LPS Induced Human Skin Fibroblast Cell Line

2021 ◽  
Vol 2 (7) ◽  
pp. 01-10
Author(s):  
Siavash Hosseinpour Chermahini ◽  
Fadzilah Adibah Abdul Majid ◽  
Azila Abdul Aziz ◽  
Roya Anvari
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Skin Fibroblast ◽  
Skin Barrier ◽  
Fibroblast Cell ◽  
Skin Inflammation ◽  
Human Skin Fibroblast ◽  
Necrosis Factor Alpha ◽  
Tnf Α

The topical delivery of bromelain as an anti-inflammatory solution for skin inflammation has attracted the attention of researchers. Due to the skin barrier issue, a new method was designed for the effective delivery of specific doses of bromelain to the desired action sites. A niosome was selected as a novel and practical transdermal vehicle for the delivery of bromelain to inflamed sites. In this regard, a lipopolysaccharide (LPS)-induced human skin fibroblast (HSF1184) cell line was assembled in-vitro as a simulated model. The levels of interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-α), the two immune-modulatory regulators of cell responses to inflammation, were measured to determine the response towards the niosome-encapsulated bromelain treatment. The results showed that the niosome-encapsulated bromelain significantly reduced the levels of IL-6 and TNF-α compared to the non-encapsulated bromelain, the vehicle (niosome) and the control.

P256 electroperturbation of the human skin barrier in vitro: effects on stratum corneum resistance, capacitance, lipid ordering and ultrastructure

1994 ◽  
Vol 2 (1-2) ◽  
pp. 182
Author(s):  
W.H.M. Craane-van Hinsberg ◽  
F. Spies ◽  
G.S. Gooris ◽  
J.A. Bouwstra ◽  
J.C. Verhoef ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Skin Barrier ◽  
In Vitro Effects

scholarly journals Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents

2021 ◽  
Vol 22 (11) ◽  
pp. 5790
Author(s):  
Arnout Mieremet ◽  
Richard W. J. Helder ◽  
Andreea Nadaban ◽  
Walter A. Boiten ◽  
Gert S. Gooris ◽  
...  
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Skin Barrier ◽  
Collagen Matrix ◽  
Optimization Approach ◽  
Skin Equivalents ◽  
Barrier Formation ◽  
Epidermal Morphogenesis

In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions. Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects. However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised. Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR). In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches. The collagen–chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization. Importantly, barrier functionality was improved in the corresponding approach. In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.

Electroperturbation of the human skin barrier in vitro: II. Effects on stratum corneum lipid ordering and ultrastructure

1997 ◽  
Vol 37 (3) ◽  
pp. 200-213 ◽  
Author(s):  
Ine W.H.M. Craane-Van Hinsberg ◽  
J. Coos Verhoef ◽  
Ferry Spies ◽  
Joke A. Bouwstra ◽  
Gert S. Gooris ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Skin Barrier ◽  
Stratum Corneum Lipid

scholarly journals Modulation of the Lipid Profile of Reconstructed Skin Substitutes after Essential Fatty Acid Supplementation Affects Testosterone Permeability

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1142 ◽  
Author(s):  
Mélissa Simard ◽  
Pierre Julien ◽  
Julie Fradette ◽  
Roxane Pouliot
Keyword(s):  
Benzoic Acid ◽  
Human Skin ◽  
Barrier Function ◽  
Percutaneous Absorption ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Permeability ◽  
Skin Substitutes ◽  
Reconstructed Human Skin

Skin models with efficient skin barrier function are required for percutaneous absorption studies. The contribution of media supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) to the development of the skin barrier function of in vitro skin models remains incompletely understood. To investigate whether PUFAs, alpha-linolenic acid (ALA, n-3 PUFA) and linoleic acid (LA, n-6 PUFA), could enhance the impermeability of a three-dimensional reconstructed human skin model, skin substitutes were produced according to the self-assembly method using culture media supplemented with either 10 μM ALA or 10 μM LA. The impact of PUFAs on skin permeability was studied by using a Franz cell diffusion system to assess the percutaneous absorption of testosterone and benzoic acid. Our findings showed that ALA supplementation induced a decrease in the absorption of testosterone, while LA supplementation did not significantly influence the penetration of testosterone and benzoic acid under present experimental conditions. Both ALA and LA were incorporated into phospholipids of the skin substitutes, resulting in an increase in n-3 total PUFAs or n-6 total PUFAs. Collectively, these results revealed the under-estimated impact of n-3 PUFA supplementation as well as the importance of the n-6 to n-3 ratio on the formation of the skin barrier of in vitro reconstructed human skin models.

scholarly journals Optimization and Evaluation of the In Vitro Permeation Parameters of Topical Products with Non-Steroidal Anti-Inflammatory Drugs through Strat-M® Membrane

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1305
Author(s):  
Bartłomiej Milanowski ◽  
Hanna Wosicka-Frąckowiak ◽  
Eliza Główka ◽  
Małgorzata Sosnowska ◽  
Stanisław Woźny ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Barrier ◽  
Pharmaceutical Products ◽  
Topical Formulations ◽  
In Vitro Permeation ◽  
Diffusion Characteristics ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Spray Formulation

Pharmaceutical products containing non-steroidal anti-inflammatory drugs (NSAIDs) are among the most prescribed topical formulations used for analgesic and antirheumatic properties. These drugs must overcome the skin barrier to cause a therapeutic effect. Human skin has been widely used as a model to study in vitro drug diffusion and permeation, however, it suffers from many limitations. Therefore, to perform in vitro permeation test (IVPT), we used a Strat-M® membrane with diffusion characteristics well-correlated to human skin. This study’s objective was to optimize the IVPT conditions using Plackett–Burman experimental design for bio-predictive evaluation of the in vitro permeation rates of five non-steroidal anti-inflammatory drugs (diclofenac, etofenamate, ibuprofen, ketoprofen, naproxen) across Strat-M® membrane from commercial topical formulations. The Plackett–Burman factorial design was used to screen the effect of seven factors in eight runs with one additional center point. This tool allowed us to set the sensitive and discriminative IVPT final conditions that can appropriately characterize the NSAIDs formulations. The permeation rate of etofenamate (ETF) across the Strat-M® membrane was 1.7–14.8 times faster than other NSAIDs from selected semisolids but 1.6 times slower than the ETF spray formulation.

Changes in corneocyte element concentrations occur in skin inner stratum corneum

1990 ◽  
Vol 48 (2) ◽  
pp. 146-147
Author(s):  
R. R. Warner
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Element Concentration ◽  
Skin Barrier ◽  
Barrier Properties ◽  
Brick Wall ◽  
Inorganic Elements ◽  
Dry Skin ◽  
Skin Biopsies ◽  
Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

Desmosomal distribution in the epidermis of a non-contracting human skin equivalent

1992 ◽  
Vol 50 (1) ◽  
pp. 896-897
Author(s):  
L.X. Oakford ◽  
S.D. Dimitrijevich ◽  
R. Gracy
Keyword(s):  
Human Skin ◽  
Basal Layer ◽  
Skin Equivalent ◽  
Tissue Component ◽  
Intact Skin ◽  
Similar Sequence ◽  
Sequence Of Events ◽  
Suprabasal Keratinocytes ◽  
Human Skin Equivalent

In intact skin the epidermal layer is a dynamic tissue component which is maintained by a basal layer of mitotically active cells. The protective upper epidermis, the stratum corneum, is generated by differentiation of the suprabasal keratinocytes which eventually desquamate as anuclear comeocytes. A similar sequence of events is observed in vitro in the non-contracting human skin equivalent (HSE) which was developed in this lab (1). As a part of the definition process for this model of living skin we are examining its ultrastructural features. Since desmosomes are important in maintaining cell-cell interactions in stratified epithelia their distribution in HSE was examined.

Sign in / Sign up

Export Citation Format

Share Document