At present, the relevant researches of Glulam columns in China are mainly restricted to short columns. In order to study the mechanical properties of long columns under axial loading, an experimental study on five different slenderness ratios of Larch Glulam columns was carried out. With slenderness ratio changing, the variations of experimental data such as axial strain, lateral deflection at mid-height, ultimate bearing capacity, and peak strain were comparatively analyzed. The failure pattern and failure mechanism of long columns were discussed. The results indicate that the ultimate bearing capacity of Larch Glulam columns gradually decreases as the slenderness radio increases and the failure mode is gradually converted from strength failure to instability failure. The ultimate load reduction factor is obtained by regression analysis based on the experiment results of Larch Glulam short columns. The basis for design and application of Larch Glulam columns are provided.