Stationary Phase Retention versus Peak Elution in CCC

2009 ◽  
Author(s):  
Philip Wood
Keyword(s):  
Stationary Phase ◽  
Versus Peak ◽  
Phase Retention

Americium(III)/curium(III) separation by countercurrent chromatography using malonamide extractants

2005 ◽  
Vol 93 (1) ◽  
Author(s):  
B. F. Myasoedov ◽  
T. A. Maryutina ◽  
M. N. Litvina ◽  
D. A. Malikov ◽  
Yu. M. Kulyako ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Stationary Phase ◽  
Flow Rate ◽  
Mobile Phase ◽  
Countercurrent Chromatography ◽  
Column Length ◽  
Isocratic Elution ◽  
Phase Systems ◽  
Phase Retention

AbstractThe separation of Am(III) and Cm(III) by countercurrent chromatography (CCC) was achieved using the liquid phase systems "diamide–hydrogenated tetrapropylene (TPH)–HNOThe following diamide extractants have been studied: (i) N,N´-dimethyl-N,N´-dibutyltetradecylmalonamide (DMDBTDMA), (ii) N,N´-dimethyl-N,N´-dioctylhexyl-ethoxymalonamide (DMDOHEMA) and (iii) N,N´-dimethyl-N,N´-dibutyldodecylethoxymalonamide (DMDBDDEMA). It is shown that these diamides can be used for the separation of Am(III) and Cm(III) by CCC. Increasing the column length leads to an increase of the stationary phase retention on the column while improving the Am/Cm separation. Increasing the speed of rotation of the centrifuge from 660 to 950 rpm also results in increasing the stationary phase retention but does not influence the resolution of the Am/Cm separation. Decreasing the flow rate of the mobile phase from 1.0 to 0.5 mL/min leads to a better resolution of Am and Cm separation. The best Am/Cm separation was achieved with systems based on DMDBDDEMA and DMDOHEMA in TPH using a two-layer coil column and an isocratic elution mode. The application of CCC makes it possible to separate the elements within 100 min: the Cm fraction contains 99.5% of Cm(III) and 0.6% of Am(III) inventories and the Am fraction contains 99.4% of Am(III) and 0.5% of Cm(III).

Stationary phase retention and preliminary application of a spiral disk assembly designed for high-speed counter-current chromatography

2008 ◽  
Vol 1188 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Xueli Cao ◽  
Guanghui Hu ◽  
Liangsheng Huo ◽  
Xiaoping Zhu ◽  
Ting Li ◽  
...  
Keyword(s):  
Stationary Phase ◽  
High Speed ◽  
Phase Retention ◽  
Counter Current ◽  
Preliminary Application

scholarly journals DETECTION AND PURIFICATION OF HARMFUL COMPOUNDS IN BEVERAGES USING HPLC

2016 ◽  
Vol 12 (20) ◽  
pp. 5215-5217 ◽  
Author(s):  
Rukkumani V ◽  
Saravanakumar M
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Retention Time ◽  
Mobile Phase ◽  
Migration Rate ◽  
High Performance ◽  
Human Beings ◽  
Carcinogenic Effects ◽  
Phase Retention

The presence of harmful compounds like caffeine and carbonated compounds in different beverages like soft drinks, fruit juices deserves great attention because of its toxic and carcinogenic effects on  human beings. We report on the detection and purification of those substances with the help of HPLC(High Performance  Liquid Chromatography).According to the migration rate, stationary phase and mobile phase, retention time we can extract the desire compounds. Depending upon the solvent and sample we can detect the compounds with  the  help  of the detector.The chromatogram will be  displayed and it can be viewed in the PC with the help of  Osiris  software. Compounds like Caffeine, Aspartame, Neotame, Saccharin, Maltodextrin, sucrose, fructose etc can be detected and purified. Detection and purification takes place in the column of HPLC where the process called adsorption takes place. Retention time can be calculated by the total time taken of a component that spends  in both mobile phase and stationary phase. It is always expressed in minutes

Dependence of Gas Chromatographic Retention Data of Hydrocarbons on the Film Thickness of the Polydimethylsiloxane Stationary Phase

1994 ◽  
Vol 59 (11) ◽  
pp. 2390-2396
Author(s):  
Ján Krupčík ◽  
Ivan Skačáni ◽  
Eva Benická ◽  
Pat Sandra
Keyword(s):  
Film Thickness ◽  
Stationary Phase ◽  
Aromatic Hydrocarbons ◽  
Active Sites ◽  
Capillary Columns ◽  
Retention Indices ◽  
Chromatographic Retention ◽  
Gas Liquid Chromatography ◽  
Retention Data ◽  
Phase Retention

Kovats indices of cyclic and aromatic hydrocarbons, separated by capillary gas liquid chromatography on polydimethylsiloxane capillary columns, were found to increase with increasing stationary phase film thickness. This effect is explained in terms of adsorption of the stationary phase on the active sites of the inner surface of the capillary column. Since the number of active sites is limited, the overall polarity of the polydimethylsiloxane stationary phase is better defined in columns with thick stationary phase films. Interlaboratory reproducibility of retention indices of cyclic and aromatic hydrocarbons is therefore also better when using capillary columns with thick films of the polydimethylsiloxane stationary phase. Retention data obtained on such thick-film columns are influenced by adsorption of the stationary phase on the column walls to a lesser extent than in columns with thinner films.

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