Quantitative analysis of androgen receptor messenger ribonucleic acid in developing Leydig cells and Sertoli cells by in situ hybridization.

Endocrinology ◽  
1995 ◽  
Vol 136 (9) ◽  
pp. 3856-3862 ◽  
Author(s):  
L X Shan ◽  
L J Zhu ◽  
C W Bardin ◽  
M P Hardy
Keyword(s):  
In Situ Hybridization ◽  
Androgen Receptor ◽  
Quantitative Analysis ◽  
Sertoli Cells ◽  
Ribonucleic Acid ◽  
Leydig Cells ◽  
Messenger Ribonucleic Acid

scholarly journals Prolactin Receptor Messenger Ribonucleic Acid in Normal and Neoplastic Human Pituitary Tissues1

1997 ◽  
Vol 82 (3) ◽  
pp. 963-968
Author(s):  
Long Jin ◽  
Xiang Qian ◽  
Elzbieta Kulig ◽  
Bernard W. Scheithauer ◽  
Rocio Calle-Rodrigue ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Reverse Transcription ◽  
Ribonucleic Acid ◽  
Prolactin Receptor ◽  
Pituitary Surgery ◽  
Specific Cell ◽  
Human Pituitary ◽  
Messenger Ribonucleic Acid ◽  
Reverse Transcription Pcr

Abstract We examined the specific cell types in normal human pituitaries that expressed PRL receptor (PRL-R) messenger ribonucleic acid (mRNA) by combined in situ hybridization and immunohistochemistry. The distribution of PRL-R mRNA in 28 pituitary adenomas was examined by in situ hybridization and reverse transcription-PCR in 12 cases of adenomas. In another set of experiments, 34 PRL adenomas from men, women, and bromocriptine-treated patients were analyzed for PRL-R by in situ hybridization. In the normal pituitary, PRL- and LH-producing cells had significantly more mean grain counts per cell and higher percentages of cells positive for PRL-R than GH and TSH cells. PRL-R mRNA was present in all groups of adenomas by in situ hybridization and reverse transcription-PCR. PRL adenomas had a significantly higher density of labeling compared to other adenoma types. Although there was no difference in the levels of PRL-R mRNA in PRL adenomas from men and premenopausal and postmenopausal women, patients treated with bromocriptine before pituitary surgery had significantly lower levels of PRL-R compared to all other groups. These results indicate that in the normal pituitary, PRL and LH cells have the highest level of PRL-R mRNA, whereas PRL adenomas have significantly higher levels of PRL-R mRNA than other types of adenomas, and bromocriptine treatment decreases the levels of PRL-R mRNA in PRL adenomas.

In Situ Hybridization Detection of Marked Differences in Pre-Proopiomelanocortin Messenger Ribonucleic Acid Content of Individual Corticotropes and Melanotropes*

Endocrinology ◽  
1989 ◽  
Vol 124 (3) ◽  
pp. 1359-1364 ◽  
Author(s):  
J. M. HATFIELD ◽  
D. I. DAIKH ◽  
J. P. ADELMAN ◽  
J. DOUGLASS ◽  
C. T. BOND ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Messenger Ribonucleic Acid ◽  
Hybridization Detection
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