scholarly journals Hormonal Stimulation of Human Ovarian Xenografts in Mice: Studying Folliculogenesis, Activation, and Oocyte Maturation

Endocrinology ◽  
2020 ◽  
Vol 161 (12) ◽  
Author(s):  
Monica Anne Wall ◽  
Vasantha Padmanabhan ◽  
Ariella Shikanov
Keyword(s):  
Ovarian Tissue ◽  
Oocyte Retrieval ◽  
Ovarian Tissue Cryopreservation ◽  
Hormonal Stimulation ◽  
Ovarian Tissue Transplantation ◽  
Human Ovarian Tissue ◽  
Activation Rates ◽  
Technological Improvements ◽  
Xenotransplantation Model ◽  
Tissue Cryopreservation

Abstract Ovarian tissue cryopreservation and banking provides a fertility preservation option for patients who cannot undergo oocyte retrieval; it is quickly becoming a critical component of assisted reproductive technology programs across the world. While the transplantation of cryopreserved ovarian tissue has resulted in over 130 live births, the field has ample room for technological improvements. Specifically, the functional timeline of grafted tissue and each patient’s probability of achieving pregnancy is largely unpredictable due to patient-to-patient variability in ovarian reserve, lack of a reliable method for quantifying follicle numbers within tissue fragments, potential risk of reintroduction of cancer cells harbored in ovarian tissues, and an inability to control follicle activation rates. This review focuses on one of the most common physiological techniques used to study human ovarian tissue transplantation, xenotransplantation of human ovarian tissue to mice and endeavors to inform future studies by discussing the elements of the xenotransplantation model, challenges unique to the use of human ovarian tissue, and novel tissue engineering techniques currently under investigation.

scholarly journals Comparison between Slow Freezing and Vitrification for Human Ovarian Tissue Cryopreservation and Xenotransplantation

2019 ◽  
Vol 20 (13) ◽  
pp. 3346 ◽  
Author(s):  
Sanghoon Lee ◽  
Ki-Jin Ryu ◽  
Boram Kim ◽  
Dahyeon Kang ◽  
Yoon Young Kim ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Primordial Follicle ◽  
Research Unit ◽  
University Hospital ◽  
Slow Freezing ◽  
Ovarian Tissue Cryopreservation ◽  
Ki 67 ◽  
Immunodeficient Mice ◽  
Human Ovarian Tissue ◽  
Tissue Cryopreservation

Two methods for the cryopreservation of human ovarian tissue were compared using a xenotransplantation model to establish a safe and effective cryopreservation method. Ovarian tissues were obtained from women who underwent benign ovarian surgery in the gynecology research unit of a university hospital. The tissues were transplanted into 112 ovariectomized female severe combined immunodeficient mice 4 weeks after slow freezing or vitrification cryopreservation. Tissues were retrieved 4 weeks later. Primordial follicular counts decreased after cryopreservation and xenotransplantation, and were significantly higher in the slow freezing group than in the vitrification group (p < 0.001). Immunohistochemistry and TUNEL assay showed that the Ki-67 and CD31 markers of follicular proliferation and angiogenesis were higher in the slow freezing group (p < 0.001 and p = 0.006, respectively) and DNA damage was greater in the vitrification group (p < 0.001). Western blotting showed that vitrification increased cellular apoptosis. Anti-Müllerian hormone expression was low in transplanted samples subjected to both cryopreservation techniques. Electron microscopy revealed primordial follicle deformation in the vitrification group. Slow freezing for ovarian tissue cryopreservation is superior to vitrification in terms of follicle survival and growth after xenotransplantation. These results will be useful for fertility preservation in female cancer patients.

scholarly journals Human ovarian tissue cryopreservation: indications and feasibility

2002 ◽  
Vol 17 (6) ◽  
pp. 1447-1452 ◽  
Author(s):  
Catherine Poirot ◽  
Marie-Cécile Vacher-Lavenu ◽  
Pierre Helardot ◽  
Juliette Guibert ◽  
Laurence Brugières ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Ovarian Tissue Cryopreservation ◽  
Human Ovarian Tissue ◽  
Tissue Cryopreservation ◽  
Human Ovarian Tissue Cryopreservation

scholarly journals Optimization of protocols for human ovarian tissue cryopreservation with sucrose, 1,2-propanediol and human serum

2010 ◽  
Vol 21 (6) ◽  
pp. 819-828 ◽  
Author(s):  
Raffaella Fabbri ◽  
Gianandrea Pasquinelli ◽  
Declan Keane ◽  
Valentina Magnani ◽  
Roberto Paradisi ◽  
...  
Keyword(s):  
Human Serum ◽  
Ovarian Tissue ◽  
Ovarian Tissue Cryopreservation ◽  
Human Ovarian Tissue ◽  
Tissue Cryopreservation ◽  
Human Ovarian Tissue Cryopreservation

scholarly journals Clinically applied procedures for human ovarian tissue cryopreservation result in different levels of efficacy and efficiency

2016 ◽  
Vol 33 (12) ◽  
pp. 1605-1614 ◽  
Author(s):  
Lobke Bastings ◽  
Johan R. Westphal ◽  
Catharina C. M. Beerendonk ◽  
Ruud L. M. Bekkers ◽  
Petra L. M. Zusterzeel ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Ovarian Tissue Cryopreservation ◽  
Human Ovarian Tissue ◽  
Tissue Cryopreservation ◽  
Different Levels ◽  
Human Ovarian Tissue Cryopreservation

scholarly journals FERTILITY PRESERVATION: Follicle reserve loss in ovarian tissue transplantation

Reproduction ◽  
2019 ◽  
Vol 158 (5) ◽  
pp. F35-F44 ◽  
Author(s):  
Hadassa Roness ◽  
Dror Meirow
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Young Patients ◽  
Ovarian Tissue Cryopreservation ◽  
Ischemic Damage ◽  
Pharmacological Agents ◽  
Ovarian Tissue Transplantation ◽  
After Cancer ◽  
Tissue Cryopreservation ◽  
Follicle Activation

Ovarian tissue cryopreservation and transplantation (OTCP-TP) has progressed over the past decade from a revolutionary experimental procedure to a well-accepted treatment in many centers for young patients with a high risk of ovarian failure after cancer treatment. The procedure is remarkably successful, with studies reporting return of ovarian function in up to 95% of graft recipients and pregnancy rates of between 30 and 50%. The most significant limitation of OTCP-TP is the massive loss of follicles that occurs following transplantation, which is primarily attributed to ischemic damage and follicle activation. We review the current approaches to reducing follicle loss and maximizing graft lifespan via pharmacological agents which reduce ischemic damage and follicle activation. We further discuss the value and disadvantage of inducing follicle activation in the graft as a means of generating mature follicles in the immediate short term.

Slow-freezing versus vitrification for human ovarian tissue cryopreservation

2014 ◽  
Vol 291 (2) ◽  
pp. 419-426 ◽  
Author(s):  
Silke Klocke ◽  
Nana Bündgen ◽  
Frank Köster ◽  
Ursula Eichenlaub-Ritter ◽  
Georg Griesinger
Keyword(s):  
Ovarian Tissue ◽  
Slow Freezing ◽  
Ovarian Tissue Cryopreservation ◽  
Human Ovarian Tissue ◽  
Tissue Cryopreservation ◽  
Human Ovarian Tissue Cryopreservation
Sign in / Sign up

Export Citation Format

Share Document