scholarly journals Insulin Receptor Isoforms and Insulin-Like Growth Factor Receptor in Human Follicular Cell Precursors from Papillary Thyroid Cancer and Normal Thyroid

2011 ◽  
Vol 96 (3) ◽  
pp. 766-774 ◽  
Author(s):  
Roberta Malaguarnera ◽  
Francesco Frasca ◽  
Aldo Garozzo ◽  
Fiorenza Gianì ◽  
Giuseppe Pandini ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Growth Factor ◽  
Insulin Receptor ◽  
Papillary Thyroid Cancer ◽  
Growth Factor Receptor ◽  
Insulin Like Growth Factor ◽  
Papillary Thyroid ◽  
Normal Thyroid ◽  
Receptor Isoforms ◽  
Insulin Receptor Isoforms

scholarly journals Insulin Receptor Isoforms and Insulin Receptor/Insulin-Like Growth Factor Receptor Hybrids in Physiology and Disease

2009 ◽  
Vol 30 (6) ◽  
pp. 586-623 ◽  
Author(s):  
Antonino Belfiore ◽  
Francesco Frasca ◽  
Giuseppe Pandini ◽  
Laura Sciacca ◽  
Riccardo Vigneri
Keyword(s):  
Growth Factor ◽  
Insulin Receptor ◽  
Growth Factor Receptor ◽  
Insulin Like Growth Factor ◽  
Receptor Isoforms ◽  
Insulin Receptor Isoforms

scholarly journals INSULIN-LIKE GROWTH FACTORS IN THE SERUM OF PATIENTS WITH PAPILLARY THYROID CANCER

2021 ◽  
Vol 74 (8) ◽  
pp. 1925-1930
Author(s):  
Nataliia S. Pidchenko ◽  
Mykola V. Krasnoselskyi ◽  
Nataliia A. Mitriaieva ◽  
Lidiya V. Grebenik ◽  
Olha M. Astapieva ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Growth Factors ◽  
Growth Factor ◽  
Blood Serum ◽  
Papillary Thyroid Cancer ◽  
The Body ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Papillary Thyroid ◽  
Insulin Like Growth Factors

The aim is to study the level of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-2 (IGF-2) in the blood serum of patients with papillary thyroid cancer, depending on the main clinical and morphological features of the disease. Materials and methods: The material was the information about 60 patients with papillary thyroid cancer (group 1). In group 2 there were 10 patients without oncopathology. All patients underwent clinical examination after total thyroidectomy before special treatment (radioiodine therapy): ultrasound diagnosis of the neck, confirmed diagnosis of papillary thyroid cancer by morphological examination of operative material. All patients underwent anthropometric studies (height, weight), on the basis of which the body mass index (BMI) was calculated. The study program also included determination of the level of thyroid-stimulating hormone of the pituitary gland (TSH), thyroglobulin (TG), antibodies to thyroglobulin (AB-TG). It was also determined the serum glucose level. In order to assess insulin resistance, the HOMA-IR index was calculated. All patients were tested for serum IGF-1 and IGF-2. Results: In the blood serum of patients with papillary thyroid cancer in 63% of patients the level of IGF-1 and in 85% – IGF-2 was probably higher than in the control group. There is a relationship between the level of IGF-1, IGF-2 and elevated level of proliferating factor – insulin in the serum of patients with papillary thyroid cancer. This may indicate an aggressive potential of the disease (i.e. clinical data on the prevalence of papillary thyroid cancer coincide with laboratory data). There was found a relationship between the expression of IGF-1, IGF-2 and insulin: at elevated levels of insulin> 24.9 μIU/ml, IGF-1 increases 4.2 times, and IGF-2 – 2.5 times. Evaluation of the relationship between the level of IGF-1 and IGF-2 and cervical lymph node involvement shows that in the absence of lesion (N0) there is an increase in these indicators by 2.2 and 1.8 times, respectively. Conclusions: The signaling system of insulin-like growth factors (IGF-1 and IGF-2) plays an important role in the occurrence and progression of malignant tumors. It is especially true for papillary thyroid cancer, so its components can be considered as potential diagnostic and prognostic markers of the disease and targets for anticancer therapy.

Insulin-like growth factor (IGF)-I A- and B-domain analogues with altered type 1 IGF and insulin receptor binding specificities

1996 ◽  
Vol 17 (3) ◽  
pp. 237-246 ◽  
Author(s):  
G K Shooter ◽  
B Magee ◽  
M A Soos ◽  
G L Francis ◽  
K Siddle ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Receptor ◽  
Receptor Binding ◽  
Insulin Like Growth Factor ◽  
Receptor Isoforms ◽  
Insulin Receptor Isoforms ◽  
Igf I ◽  
Igf Receptor ◽  
Specificity Of Binding

ABSTRACT Insulin-like growth factor-I (IGF-I) analogues were produced with the aim of identifying IGF-I residues that contribute to the specificity of binding to the type 1 IGF receptor as opposed to the insulin receptor. Receptor binding properties of a series of A- and B-domain analogues were compared using rat L6 myoblasts, soluble human IGF type 1 receptors and soluble human insulin receptor isoforms HIR-A (−Ex11) and HIR-B (+Ex11). IGF-I analogues, [Leu8] IGF-I and [Phe59] IGF-I, were shown to exhibit respectively, a 28- and 17-fold decrease in affinity for the HIR-A with only a 6- and 5-fold decrease in affinity for the human IGF type 1 receptor. In contrast, the analogue [His4] IGF-I was equipotent to IGF-I in binding to the soluble type 1 IGF receptor while showing 7-fold and 4-fold increases in HIR-A and HIR-B binding respectively. Furthermore, [Leu62] IGF-I was 8-fold less potent than IGF-I in soluble IGF type 1 receptor binding but only showed a 2-fold decrease in HIR-A and HIR-B binding. Our study supports the conclusion that the co-evolution of the IGF-I and insulin receptor/ligand systems has resulted in subtle structural differences in the A- and B-regions of each ligand important for defining receptor binding specificity.

Molecular imaging of platelet-derived growth factor receptor-alpha (PDGFRα) in papillary thyroid cancer using immuno-PET

2018 ◽  
Vol 58 ◽  
pp. 51-58 ◽  
Author(s):  
Michael Wagner ◽  
Melinda Wuest ◽  
Ingrit Hamann ◽  
Ana Lopez-Campistrous ◽  
Todd P.W. McMullen ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Molecular Imaging ◽  
Growth Factor ◽  
Papillary Thyroid Cancer ◽  
Growth Factor Receptor ◽  
Platelet Derived Growth Factor ◽  
Papillary Thyroid ◽  
Receptor Alpha ◽  
Factor Receptor Alpha

scholarly journals Development of a Quantitative PCR Assay for Detection of Human Insulin-Like Growth Factor Receptor and Insulin Receptor Isoforms

Endocrinology ◽  
2016 ◽  
Vol 157 (4) ◽  
pp. 1702-1708 ◽  
Author(s):  
Clare A. Flannery ◽  
Anne M. Rowzee ◽  
Gina H. Choe ◽  
Farrah L. Saleh ◽  
Caitlin C. Radford ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Receptor ◽  
Splice Variants ◽  
Growth Factor Receptor ◽  
Cell Types ◽  
Receptor Expression ◽  
Molecular Signature ◽  
Insulin Like Growth Factor ◽  
Insulin Receptor Isoforms ◽  
Signaling Receptors

Abstract The biological activity of insulin and the insulin-like growth factor (IGF) ligands, IGF-I and IGF-II, is based in part on the relative abundance and distribution of their target receptors: the insulin receptor (IR) splice variants A (IR-A) and B (IR-B) and IGF 1 receptor (IGF-1R). However, the relative quantity of all three receptors in human tissues has never been measured together on the same scale. Due to the high homology between insulin receptor (IR)-A and IR-B proteins and lack of antibodies that discern the two IR splice variants, their mRNA sequence is the most reliable means of distinguishing between the receptors. Hence, highly specific primers for IR-A, IR-B, and IGF-1R mRNA were designed to accurately detect all three receptors by quantitative RT-PCR and enable direct quantification of relative receptor expression levels. A standard concentration curve of cDNA from each receptor was performed. Assay specificity was tested using competition assays and postamplification analysis by gel electrophoresis and cloning. Forward and reverse primer concentrations were optimized to ensure equal efficiencies across primer pairs. This assay enables a specific molecular signature of IGF/insulin signaling receptors to be assayed in different tissues, cell types, or cancers.

Platelet-derived growth factor receptor-α promotes lymphatic metastases in papillary thyroid cancer

2012 ◽  
Vol 228 (2) ◽  
pp. 241-250 ◽  
Author(s):  
Jingdong Zhang ◽  
Peng Wang ◽  
Mark Dykstra ◽  
Pascale Gelebart ◽  
David Williams ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Growth Factor ◽  
Papillary Thyroid Cancer ◽  
Growth Factor Receptor ◽  
Platelet Derived Growth Factor ◽  
Papillary Thyroid ◽  
Lymphatic Metastases
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