High levels of human chorionic gonadotropin retard first trimester trophoblast invasion in vitro by decreasing urokinase plasminogen activator and collagenase activities

1993 ◽  
Vol 77 (6) ◽  
pp. 1506-1511 ◽  
Author(s):  
S. Yagel
Keyword(s):  
Plasminogen Activator ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
First Trimester ◽  
Urokinase Plasminogen Activator ◽  
Trophoblast Invasion

scholarly journals Human Chorionic Gonadotropin Produced by the Invasive Trophoblast But Not the Villous Trophoblast Promotes Cell Invasion and Is Down-Regulated by Peroxisome Proliferator-Activated Receptor-γ

Endocrinology ◽  
2007 ◽  
Vol 148 (10) ◽  
pp. 5011-5019 ◽  
Author(s):  
Karen Handschuh ◽  
Jean Guibourdenche ◽  
Vassilis Tsatsaris ◽  
Mickaël Guesnon ◽  
Ingrid Laurendeau ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Cell Invasion ◽  
Human Chorionic Gonadotropin ◽  
Primary Cultures ◽  
Trophoblast Invasion ◽  
Peroxisome Proliferator ◽  
Dependent Manner ◽  
Peroxisome Proliferator Activated Receptor ◽  
Villous Trophoblast

A critical step in the establishment of human pregnancy is the invasion of the uterus wall by extravillous cytotrophoblasts (EVCTs) during the first trimester. It is well established that human chorionic gonadotropin hormone (hCG) is secreted by the endocrine syncytiotrophoblast (ST) into the maternal compartment. We recently reported that invasive EVCTs also produce hCG, suggesting an autocrine role in the modulation of trophoblast invasion. Here we analyzed the role of hCG secreted in vitro by primary cultures of invasive EVCT and noninvasive ST. We first demonstrated that LH/CG receptor was present in EVCTs in situ and in vitro as well as in an EVCT cell line (HIPEC65). We next showed that hCG secreted by EVCTs stimulated progesterone secretion by MA10 cells in a concentration-dependent manner. Incubation of HIPEC65 with EVCT supernatants induced a 10-fold increase in cell invasion, whereas ST supernatants had no effect. This stimulating effect was strongly decreased when hCG was depleted from EVCT supernatants containing a large amount of the hyperglycosylated form of hCG, which is almost undetectable in ST supernatants. Finally, we investigated the regulation of hCG expression by peroxisome proliferator-activated receptor (PPAR)-γ, a nuclear receptor shown to inhibit trophoblast invasion. Activation of PPARγ decreased α- and β-subunit transcript levels and total hCG secretion in primary EVCTs. Our results offer the first evidence that hCG secreted by the invasive trophoblast, likely the hyperglycosylated form of hCG, but not by the syncytiotrophoblast, promotes trophoblast invasion and may be a PPARγ target gene in trophoblast invasion process.

Hyperglycosylated chorionic gonadotropin – prognostic criteria development of complications of pregnancy

2017 ◽  
pp. 82-85
Author(s):  
N.V. Pehnyo ◽  
Keyword(s):  
Pregnant Women ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Prognostic Significance ◽  
Neonatal Morbidity ◽  
First Trimester ◽  
Trophoblast Invasion ◽  
Placental Dysfunction ◽  
Hyperglycosylated Human Chorionic Gonadotropin ◽  
First Trimester Of Pregnancy

The objective: to assess the prognostic significance of the level of serum hyperglycosylated human chorionic gonadotropin (gHCG) reative to the development of pregnancy complications that lead to impaired fetal conditions. Materials and methods. 382 pregnant women were examined who were on the register, were hospitalized in case of complications of pregnancy and gave birth to KCMH №2 (Kiev). All pregnant women were evaluated serum gCGG in the period of 8-10 weeks gestation, evaluated the course and result of pregnancy. Results. Reduction in the level of gHCG is a marker of insufficient trophoblast invasion, which is likely to lead to the formation of placental dysfunction and the development of a variety of obstetric pathology complicating the course of pregnancy and a risk factor for antenatal loss and neonatal morbidity. The conclusion. Determination of the level of hyperglycosylated human chorionic gonadotropin in the first trimester of pregnancy will make it possible to identify pregnant women at risk for the formation of placental dysfunction and propose a set of monitoring activities aimed at early detection of violations of pregnancy and their timely removal. This helps prevent the formation of severe pathological conditions during pregnancy in both the mother and the fetus, which corresponds to the current trends in the development of medical care, namely: prediction, prevention and individual approach. Key words: hyperglycosylated chorionic gonadotrophin, complications of pregnancy, disturbed fetal condition.

Sign in / Sign up

Export Citation Format

Share Document