FSH AND LH RESPONSE TO LUTEINIZING HORMONE-RELEASING FACTOR IN PREPUBERTAL AND PUBERTAL CHILDREN, ADULT MALES AND PATIENTS WITH HYPOGONADOTROPIC AND HYPERGONADOTROPIC HYPOGONADISM

1972 ◽  
Vol 35 (6) ◽  
pp. 926-930 ◽  
Author(s):  
J.C. Roth ◽  
R.P. Kelch ◽  
S.L. Kaplan ◽  
M.M. Grumbach
Keyword(s):  
Luteinizing Hormone ◽  
Adult Males ◽  
Hypergonadotropic Hypogonadism

THE ROLE OF PROLACTIN IN THE REGULATION OF TESTIS FUNCTION: THE SYNERGISTIC EFFECTS OF PROLACTIN AND LUTEINIZING HORMONE ON THE INCORPORATION OF [1-14C]ACETATE INTO TESTOSTERONE AND CHOLESTEROL BY TESTES FROM HYPOPHYSECTOMIZED RATS IN VITRO

1972 ◽  
Vol 53 (2) ◽  
pp. 223-230 ◽  
Author(s):  
A. A. HAFIEZ ◽  
A. BARTKE ◽  
C. W. LLOYD
Keyword(s):  
Luteinizing Hormone ◽  
Synergistic Effects ◽  
Male Rats ◽  
Gas Liquid Chromatography ◽  
Adult Males ◽  
Bicarbonate Buffer ◽  
A Value ◽  
Hypophysectomized Rats ◽  
Ovine Prolactin

SUMMARY Hypophysectomized adult male rats were treated twice daily for 3½ days with 0·9% NaCl solution, 200 μg ovine prolactin, 5 μg ovine luteinizing hormone (LH), or 200 μg prolactin +5 μg LH, starting 18 days after the operation. Minced testes from these animals were incubated in Krebs—Ringer bicarbonate buffer containing [1-14C]acetate for 3 h at 37 °C. The conversion of acetate into testosterone was calculated. It was not changed by prolactin treatment, was increased by LH (P < 0·01), and further increased by prolactin + LH (P < 0·01) to approximately the level observed in intact adult controls. The amount of testosterone in some of the incubates was measured by gas liquid chromatography. It was not changed in prolactin-treated animals and was increased in LH-treated animals (P < 0·02 compared with hypophysectomized controls). Treatment with prolactin+LH resulted in an increase in testosterone above that after treatment with LH alone (P < 0·01) and to a value similar to that in intact adult males. The incorporation of acetate into esterified cholesterol was decreased (P < 0·05) in groups treated with LH or prolactin + LH in which testosterone synthesis was increased. The incorporation of acetate into free cholesterol was not changed by any of the treatments. These results demonstrate synergistic action of prolactin and LH on the synthesis of testosterone in vitro confirming our earlier suggestions.

EFFECTS OF PROLACTIN AND LUTEINIZING HORMONE ON THE CHOLESTEROL STORES IN THE MOUSE TESTIS

1971 ◽  
Vol 49 (2) ◽  
pp. 317-324 ◽  
Author(s):  
A. BARTKE
Keyword(s):  
Luteinizing Hormone ◽  
Individual Variation ◽  
Free Cholesterol ◽  
Testosterone Propionate ◽  
Follicle Stimulating Hormone ◽  
Mouse Testis ◽  
Cholesterol Esters ◽  
Adult Males ◽  
Male Mice ◽  
Androgen Synthesis

SUMMARY In intact adult male mice three injections, 2 h apart, of 3 μg luteinizing hormone (LH), 15 μg LH or 100 μg follicle-stimulating hormone (FSH) decreased the concentration of esterified cholesterol in the testes. Treatment with 35 μg testosterone propionate (TP)/day or 4 mg aminoglutethimide/day increased the concentration of esterified cholesterol. Single heterotopic pituitary homografts increased the concentration of esterified cholesterol; injecting 7·5 i.u. prolactin twice daily had similar effect. The concentration of free cholesterol showed little individual variation and little or no response to any of the above treatments. The concentration of testicular total cholesterol was increased in hypophysectomized mice. Administration of 10 μg LH or 50 μg TP daily for 28 days reduced the concentration of both esterified and free cholesterol in these animals to approximately the levels found in intact adult males. Prolactin (12 i.u./day) given alone or with TP had no effect, but it increased the concentration of esterified cholesterol in LH-treated animals. It is concluded that in the mouse testis the concentration of esterified, but not free, cholesterol tends to fluctuate with the apparent changes in the rate of androgen synthesis and that prolactin promotes accumulation of cholesterol esters. This could explain the synergism between prolactin and LH in stimulating spermatogenesis and the growth of androgen dependent tissues.

THE ROLE OF PROLACTIN IN THE REGULATION OF TESTIS FUNCTION: THE EFFECTS OF PROLACTIN AND LUTEINIZING HORMONE ON THE PLASMA LEVELS OF TESTOSTERONE AND ANDROSTENEDIONE IN HYPOPHYSECTOMIZED RATS

1972 ◽  
Vol 52 (2) ◽  
pp. 327-331 ◽  
Author(s):  
A. A. HAFIEZ ◽  
C. W. LLOYD ◽  
A. BARTKE
Keyword(s):  
Luteinizing Hormone ◽  
Plasma Levels ◽  
Treated Group ◽  
Adult Males ◽  
Intact Control ◽  
Testosterone Levels ◽  
Hypophysectomized Rats ◽  
Slight Rise ◽  
Very High

SUMMARY Testosterone and androstenedione levels in the plasma of hypophysectomized rats were measured by a protein-binding method (sensitivity 150 pg testosterone; precision, 10%). Testosterone levels in the plasma of saline-treated hypophysectomized rats were undetectable (<0·3 ng/ml). Administration of 200 μg prolactin twice daily s.c. for a period of 3½ days caused a slight rise in testosterone in four rats (0·61 ± 0·35 ng/ml) while 5 μg luteinizing hormone (LH) administered in the same regimen increased testosterone levels to 1·42 ± 0·35 ng/ml (P < 0·05). Administration of prolactin ± LH (same doses) increased testosterone levels to 5·07 ± 0·51 ng/ml which is significantly higher than when LH was given alone and exceeds testosterone levels in intact adult males (4·12 ± 0·60 ng/ml). Androstenedione levels in the plasma of saline-treated hypophysectomized rats were, in contrast to testosterone levels, very high and averaged 3·37 ± 0·60 ng/ml. This level is higher than that in saline-treated intact controls (1·89 ± 0·36 ng/ml). Treatment with prolactin, LH or prolactin + LH induced insignificant changes in androstenedione levels which were 1·80 ± 0·33, 2·02 ± 0·28 and 4·22 ± 0·75 ng/ml, respectively. The ratio testosterone: androstenedione was calculated to be 0·37 ± 0·08 in the prolactin-treated group, 0·76 ± 0·20 in the LH-treated group and 1·47 ± 0·64 in the prolactin + LH-treated group compared with 2·36 ± 0·28 in intact control males.

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

The associations of age and dentition status with caries treatment in adult males

1988 ◽  
Vol 52 (11) ◽  
pp. 609-614
Author(s):  
JW Reinhardt ◽  
CW Douglass ◽  
JE Alman ◽  
HH Chauncey
Keyword(s):  
Adult Males ◽  
Caries Treatment
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