scholarly journals Polymerase Chain Reaction-Based Microsatellite Polymorphism Analysis of Follicular and Hürthle Cell Neoplasms of the Thyroid1

1998 ◽  
Vol 83 (6) ◽  
pp. 2036-2042
Author(s):  
Dorry L. Segev ◽  
Motoyasu Saji ◽  
Grace S. Phillips ◽  
William H. Westra ◽  
Yumi Takiyama ◽  
...  
Keyword(s):  
Surgical Management ◽  
Frozen Section ◽  
Follicular Adenoma ◽  
Follicular Carcinoma ◽  
Thyroid Neoplasms ◽  
Needle Aspiration ◽  
Microsatellite Polymorphism ◽  
Polymorphism Analysis ◽  
Exact Test ◽  
Allelic Alteration

Follicular and Hürthle cell carcinomas of the thyroid cannot be differentiated from adenomas by either preoperative fine needle aspiration or intraoperative frozen section examination, and yet there exist potentially significant differences in the recommended surgical management. We examined, by PCR-based microsatellite polymorphism analysis, DNA obtained from 83 thyroid neoplasms [22 follicular adenomas, 29 follicular carcinomas, 20 Hürthle cell adenomas (HA), and 12 Hürthle cell carcinomas (HC)] to determine whether a pattern of allelic alteration exists that could help distinguish benign from malignant lesions. Alterations were found in only 7.5% of informative PCR reactions from follicular neoplasms, whereas they were found in 23.3% of reactions from Hürthle cell neoplasms. Although there were no significant differences between follicular adenoma and follicular carcinoma, HC demonstrated a significantly greater percentage of allelic alteration than HA on chromosomal arms 1q (P < 0.001) and 2p (P < 0.05) by Fisher’s exact test. The documentation of an alteration on either 1q or 2p was 100% sensitive and 65% specific in the detection of HC (P < 0.0005, by McNemar’s test). In conclusion, PCR-based microsatellite polymorphism analysis may be a useful technique in distinguishing HC from HA. Potentially, the application of this technique to aspirated material may allow this distinction preoperatively and thus facilitate more optimal surgical management. Consistent regions of allelic alteration may also indicate the locations of critical genes, such as tumor suppressor genes or oncogenes, that are important in the progression from adenoma to carcinoma. Finally, this study demonstrates that Hürthle cell neoplasms, now considered variants of follicular neoplasms, differ significantly from follicular neoplasms on a molecular level.

scholarly journals Better Understanding in the Differentiation of Thyroid Follicular Adenoma, Follicular Carcinoma, and Follicular Variant of Papillary Carcinoma: A Retrospective Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Jung Hyun Yoon ◽  
Eun-Kyung Kim ◽  
Ji Hyun Youk ◽  
Hee Jung Moon ◽  
Jin Young Kwak
Keyword(s):  
Differential Diagnosis ◽  
Frozen Section ◽  
Follicular Adenoma ◽  
Follicular Carcinoma ◽  
Needle Aspiration ◽  
Follicular Neoplasm ◽  
Papillary Thyroid ◽  
Follicular Variant ◽  
Fine Needle

Background.To evaluate the role of ultrasonography (US), US-guided fine-needle aspiration (USFNA) and intraoperative frozen section (FS) in follicular neoplasm.Methods. US features, USFNA cytology, and FS results were compared based on the pathology results of patients with follicular adenoma (FA), follicular carcinoma (FC), and follicular variant of papillary thyroid carcinoma (FVPTC).Results. FC and FVPTC showed significantly higher rates of suspicious US features (P<0.05) and positive findings on either US or cytology, 80.0% and 90.7%, compared to FA, 64.5% (P=0.001). Intraoperative FS showed higher malignant rates in FVPTC and FC (81.8% and 75.0%, resp.), compared to FA (3.8%,P<0.001).Conclusion. Suspicious US features were more significantly seen in FC and FVPTC compared to FA. Intraoperative FS is useful in the differential diagnosis of these lesions and supplements cytology results of USFNA.

The Thyroid Gland: Recurring Problems in Histologic and Cytologic Evaluation

2008 ◽  
Vol 132 (4) ◽  
pp. 622-632
Author(s):  
William C. Faquin
Keyword(s):  
Thyroid Gland ◽  
Follicular Adenoma ◽  
Endocrine System ◽  
Follicular Carcinoma ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Poorly Differentiated Carcinoma ◽  
Different Types ◽  
Poorly Differentiated ◽  
Common Malignancy

Abstract Nodules of the thyroid gland are frequently encountered, occurring in up to 7% of the population, and although most of these nodules are benign, carcinomas of the thyroid gland are the most common malignancy of the endocrine system. Although the different types of thyroid carcinoma are few, a wide variety of recurring problems exists in both their histologic and cytologic evaluation. Here, I will review a selected group of problematic areas, including unusual histologic variants of follicular adenoma, criteria for diagnosing minimally invasive follicular carcinoma, the use of fine-needle aspiration as a screening test for follicular neoplasia, challenging variants of papillary carcinoma, and features of poorly differentiated carcinoma.

scholarly journals Diagnostic Pitfall of Thyroid Fine-needle Aspiration Induced Fibrosis: Follicular Adenoma Mimicking Medullary Thyroid Carcinoma in Frozen Section

2020 ◽  
Author(s):  
Woo Sung Moon ◽  
Myoung Jae Kang ◽  
Hyun Jo Youn ◽  
Kyoung Min Kim
Keyword(s):  
Thyroid Carcinoma ◽  
Medullary Thyroid Carcinoma ◽  
Fine Needle Aspiration ◽  
Frozen Section ◽  
Follicular Adenoma ◽  
Needle Aspiration ◽  
Medullary Thyroid ◽  
Fine Needle ◽  
Histological Alterations ◽  
Diagnostic Pitfall

Abstract Background:Fine-needle aspiration (FNA) is a frequently utilized method for the diagnosis of thyroid nodules. Although the technique has clear advantages, the injury caused by the aspiration needle can induce various histological alterations. Herein, we report a case of follicular adenoma showing histological alterations possibly caused by FNA biopsy. Furthermore, the histological appearance of the lesion mimicked those of medullary thyroid carcinoma, particularly in the frozen section. Case presentation: Ultrasonography of a thyroid nodule in a 39-year-old man revealed a mass (2.2cm in diameter) in the right thyroid lobe. FNA was performed three times on the mass, and the results of the cytology were atypia of undetermined significance. Thereafter, the patient underwent right hemithyroidectomy. The histological findings of the operative frozen section analysis indicated medullary thyroid carcinoma. However, after evaluation and immunohistochemical staining of the permanent section, the mass was diagnosed as follicular adenoma with extensive fibrosis.Conclusion:The histological alterations observed in the follicular adenoma are believed to have been caused by injury during the repeated FNA procedures.

scholarly journals The Potential Diagnostic Utility of TROP-2 & C-Kit in Thyroid Neoplasms

2021 ◽  
pp. 110-123
Author(s):  
Amal Abd El-Halim El-Dakrany ◽  
Yomna Abd El-Monem Zamzam ◽  
Rania Elsayed Wasfy ◽  
Assia Mahfouz Abd El-Raouf
Keyword(s):  
Diagnostic Tool ◽  
Follicular Adenoma ◽  
Follicular Carcinoma ◽  
Thyroid Neoplasms ◽  
Common Finding ◽  
Diagnostic Utility ◽  
Large Set ◽  
Benign Thyroid ◽  
Nuclear Features ◽  
Thyroid Lesions

Background: Thyroid nodules are common finding, only 5% of nodules are malignant and the vast majority is non-neoplastic lesions or benign neoplasms. Thyroid cancer incidence is increasing faster than any other cancer types, thus representing one of the most common and clinically worrying malignant tumors of the endocrine system. Trophoblast antigen 2 (TROP2) is a transmembrane receptor glycoprotein encoded by the tumor-associated calcium signal transducer 2(Tacstd2) gene, which is located on chromosome 1p32. Although the biological function of TROP2 is unclear, accumulating evidence has demonstrated that its expression is elevated in various malignant tissues, whereas in human normal tissues relatively low or no TROP2 expression is observed. C-Kit is a type III receptor tyrosine kinase. C-Kit expression and signaling have been well characterized in several tumors, including gastrointestinal stromal tumors (GISTs). However, few studies have investigated c-Kit in the thyroid gland or in thyroid malignancies. The aim of this study was to investigate the diagnostic utility of TROP-2 on a large set of neoplastic thyroid lesions & to investigate the utility of TROP-2 & c-Kit markers to distinguish between benign and malignant thyroid neoplasms on Paraffin blocks. Methods: Immunohistochemistry for TROP2 and c-Kit was carried out on 85 different thyroid lesions (40 benign, 7 borderline and 38 malignant). Results: Malignant thyroid lesions were found to have negative expression of c-Kit in contrast to 80% of benign thyroid neoplasms. TROP2 was strong positive in 87.5% of papillary thyroid carcinomas (PTC), but there was no TROP2 expression in benign thyroid neoplasms, non-invasive follicular thyroid neoplasm with papillary like nuclear features, follicular carcinoma, anaplastic and poorly differentiated thyroid carcinoma. Conclusions: TROP2 is a good diagnostic tool for PTCs to differentiate between PTCs & other lesions with papillary like nuclear features as NIFTP, c-Kit is a good diagnostic tool for follicular adenoma & to differentiate between follicular adenoma & follicular carcinoma.

The value of frozen section in intraoperative surgical management of thyroid follicular carcinoma

Head & Neck ◽  
2003 ◽  
Vol 25 (7) ◽  
pp. 521-528 ◽  
Author(s):  
Danijel Do?en ◽  
Marko Turi? ◽  
Josip ?malcelj ◽  
Renato Janu?i? ◽  
Marija Pastor?i? Grgi? ◽  
...  
Keyword(s):  
Surgical Management ◽  
Frozen Section ◽  
Follicular Carcinoma ◽  
Thyroid Follicular Carcinoma

The Role of Fine-Needle Aspiration and Intraoperative Frozen Section in the Surgical Management of Solitary Thyroid Nodules

Surgery Today ◽  
2002 ◽  
Vol 32 (10) ◽  
pp. 857-861 ◽  
Author(s):  
Simon D. Duek ◽  
David Goldenberg ◽  
Shai Linn ◽  
Michael M. Krausz ◽  
Dan D. Hershko
Keyword(s):  
Surgical Management ◽  
Fine Needle Aspiration ◽  
Thyroid Nodules ◽  
Frozen Section ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Intraoperative Frozen Section

scholarly journals Diagnostic pitfall of thyroid fine-needle aspiration induced fibrosis: follicular adenoma mimicking medullary thyroid carcinoma in frozen section

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Woo Sung Moon ◽  
Myoung Jae Kang ◽  
Hyun Jo Youn ◽  
Kyoung Min Kim
Keyword(s):  
Thyroid Carcinoma ◽  
Medullary Thyroid Carcinoma ◽  
Fine Needle Aspiration ◽  
Frozen Section ◽  
Follicular Adenoma ◽  
Needle Aspiration ◽  
Medullary Thyroid ◽  
Fine Needle ◽  
Histological Alterations ◽  
Diagnostic Pitfall

Abstract Background Fine-needle aspiration (FNA) is a frequently utilized method for the diagnosis of thyroid nodules. Although the technique has clear advantages, the injury caused by the aspiration needle can induce various histological alterations. Herein, we report a case of follicular adenoma showing histological alterations possibly caused by FNA biopsy. Furthermore, the histological appearance of the lesion mimicked those of medullary thyroid carcinoma, particularly in the frozen section. Case presentation Ultrasonography of a thyroid nodule in a 39-year-old man revealed a mass (2.2 cm in diameter) in the right thyroid lobe. FNA was performed three times on the mass, and the results of the cytology were atypia of undetermined significance. Thereafter, the patient underwent right hemithyroidectomy. The histological findings of the operative frozen section analysis indicated medullary thyroid carcinoma. However, after evaluation and immunohistochemical staining of the permanent section, the mass was diagnosed as follicular adenoma with extensive fibrosis. Conclusion The histological alterations observed in the follicular adenoma are believed to have been caused by injury during the repeated FNA procedures.

scholarly journals Adenoma thyroid with supraclavicular tuberculous lymphadenitis Masquerading as malignancy thyroid with Metastasis - Diagnostic dilemma

2020 ◽  
Vol 8 (2) ◽  
pp. 106-108
Author(s):  
Nagendra Mahendra ◽  
Abhijeet Ingle ◽  
Dharmendra kumar borad ◽  
Majed Abdul Basit Momin
Keyword(s):  
Lymph Node ◽  
Clinical Examination ◽  
Follicular Adenoma ◽  
Clinical Importance ◽  
Follicular Carcinoma ◽  
Needle Aspiration ◽  
Tuberculous Lymphadenitis ◽  
Supraclavicular Lymph Node ◽  
Diagnostic Dilemma ◽  
Thyroid Swelling

Thyroid swelling or goitre is one of the commonest presentations among female patients more than males attending ENT out patients. The clinical importance of thyroid swelling is related to evaluating their functional status and assess,whether it is benign,malignant ,hypo or hyper active.Even if benign should look for pressure symptoms which justifies surgery.Tuberculous lymphadenitis is the most common form of extrapulmonary tuberculosis.Coexistence of thyroid swelling with supraclavicular lymph node mimicking malignancy with metastatic deposit is unusual and always to be evaluated. We report a case of a 45-year female,who on outside initial evaluation was diagnosed as follicular carcinoma of thyroid. However clinical examination revealed a palpable supraclavicular lymph node. Thus subjected to a repeat FNA (fine needle aspiration) from thyroid which revealed benign thyroid follicular adenoma and FNA from supraclavicular lymph node revealed tuberculous lymphadenitis, which was further confirmed by histological sections post operatively. This case emphasizes the need for thorough clinical examination and utility of image guided FNA to rule out the biological nature of these lesions.

scholarly journals Glutathione S-transferase T1 and M1 polymorphisms and risk of thyroid neoplasms

2003 ◽  
Vol 11 (3) ◽  
pp. 185-185
Author(s):  
Karmen Stankov ◽  
Stefano Landi ◽  
Marco Volante ◽  
Mauro Papotti
Keyword(s):  
Confidence Interval ◽  
Thyroid Carcinoma ◽  
Odds Ratio ◽  
Follicular Adenoma ◽  
Follicular Carcinoma ◽  
Thyroid Neoplasms ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Gstt1 Null Genotype ◽  
Increased Risk

Background: In order to test the possibility of association between GSTT1 and M1 (glutathione S-transferase) null allele variant, in which the entire gene is absent, and the risk of TCO (thyroid carcinoma with cell oxyphilia), the case-control study was carried out. Methods: Genotypes for GSTT1 and GSTM1 were determined by multiplex PCR in the DNA from 108 healthy individuals and in DNA from samples of thyroid tumors from 130 patients of the same race and origin as the control group (Caucasian, Italian). The following types of NMTC were analyzed: oxyphilic adenoma (OA), oxyphilic carcinoma (OC) papillary thyroid carcinoma with oxyphilic features (PTCof), follicular adenoma (FA), follicular carcinoma (FC), follicular variant of PTC (fvPTC) and classical PTC. Associations between prevalence of particular genotypes and the occurrence of TCO (versus controls) and other subtypes of NMTC were tested. Associations were quantified by calculating OR (odds ratio) with 95% confidence interval. StatGraphics Plus v. 5 software (Manugistics) was used for statistical analysis. Results: In this study of the association between the GSTT1 and M1 null genotype and the increased risk of TCO, the frequency of GSTT1 null genotype of 19.2% in cases and 15.7% in controls was found with an adjusted odds ratio (OR) of 1.4 (95% confidence interval (CI) 0.70-2.81), and the frequency of GSTM1 null genotype of 59% in cases with oxyphilic tumors and of 55.6% in controls (OR 1.24; 95% CI, 0.62-2.48). Conclusion: These results indicate that the GSTT1 and M1 null genotypes do not increase the risk of development of oxyphilic tumors, as well as other types of NMTC that have been included in this study.

Sign in / Sign up

Export Citation Format

Share Document