scholarly journals SAT-035 In Vitro Effect of Different Follicle-Stimulating Hormone Preparations on Sertoli Cells

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Giuseppe Grande ◽  
Arato Iva ◽  
Ferran Barrachina ◽  
Catia Bellucci ◽  
Lilli Cinzia ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Pcr Analysis ◽  
A Chain ◽  
Main Regulator ◽  
And Function ◽  
Vitro Effect ◽  
Stimulating Hormone

Abstract Follicle-stimulating hormone (FSH) is the main regulator of spermatogenesis and plays a key role in the development and function of the reproductive system. To assess the effects of different FSH preparations in combination with testosterone on porcine pre-pubertal Sertoli cells, we performed Real Time PCR analysis of AMH, inhibin B and FSH-r, Western blotting analysis of AKT-posphoAKT, ERK1/2-posphoERK1/2, ELISA assay for AMH and inhibin B and a high-throughput proteomic analysis.We observed that all three preparations induced a reduction of AMH in terms of mRNA and secreted protein and, an increase of inhibin B in terms of mRNA in all the formulations while, only α-follitropin induced an increase of inhibin B secreted in the culture medium. Proteomic analysis permitted us to identify 46 secreted proteins.Of those, the SPARC protein was down-regulated after the treatment with testosterone associated with α-follitropin, β-follitropin and urofollitropin (vs group stimulated with T alone). 11 proteins were up-regulated by the different FSH preparations. In detail, Hemoglobin subunit beta, TPA and TPI have been observed to be up-regulated by stimulation with testosterone in addition with α-follitropin or with β-follitropin and or with urofollitropin. All preparations induced an increase in the secreted inhibin beta A chain, but in the medium after stimulation with urofollitropin we observed an higher increase in the levels of this protein. β-follitropin, associated with testosterone, specifically induces an up-regulation of 8 specific secreted proteins.Our study, showing that the three FSH preparations were associated with different effects, could offer the opportunity to shed light inside applications to personalized reproductive medicine.

scholarly journals Effects of GH and IGF1 on Basal and FSH-Modulated Porcine Sertoli Cells In-Vitro

2019 ◽  
Vol 8 (6) ◽  
pp. 811 ◽  
Author(s):  
Rossella Cannarella ◽  
Francesca Mancuso ◽  
Rosita A. Condorelli ◽  
Iva Arato ◽  
Laura M. Mongioì ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibitory Effect ◽  
Inhibin B ◽  
Neonatal Pigs ◽  
And Function ◽  
Lines Of Evidence

Several lines of evidence suggest that insulin-like growth factor 1 (IGF1) is involved in Sertoli cell (SC) proliferation and that its receptor (IGF1R) could mediate follicle-stimulating hormone (FSH) effects. To examine the role of the growth hormone (GH)-IGF1 axis on SC function, we evaluated the effects of GH and IGF1 on basal and FSH-modulated SC proliferation, as well as on anti-Müllerian hormone (AMH) and inhibin B expression and secretion in-vitro. SCs from neonatal pigs were incubated with (1) placebo, (2) 100 nM highly purified urofollitropin (hpFSH), (3) 100 nM recombinant GH (rGH), (4) 100 nM recombinant IGF1 (rIGF1), (5) 100 nM hpFSH plus 100 nM rGH, (6) 100 nM hpFSH plus 100 nM rIGF1, for 48 h. We found that IGF1, but not FSH nor GH, stimulated SC proliferation. Furthermore, an inhibitory effect of FSH, GH and IGF1 on AMH secretion, and a stimulatory role of FSH and IGF1, but not GH, on inhibin B secretion were found. These results suggest that the GH-IGF1 axis influences basal and FSH-modulated SC proliferation and function. We speculate that SC proliferation occurring in childhood might be supported by the increased serum IGF1 levels observed during this period of life.

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

scholarly journals Inhibin B Is a More Potent Suppressor of Rat Follicle-Stimulating Hormone Release than Inhibin A in Vitro and in Vivo

Endocrinology ◽  
2009 ◽  
Vol 150 (10) ◽  
pp. 4784-4793 ◽  
Author(s):  
Yogeshwar Makanji ◽  
Peter D. Temple-Smith ◽  
Kelly L. Walton ◽  
Craig A. Harrison ◽  
David M. Robertson
Keyword(s):  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Hormone Release ◽  
Inhibin A ◽  
Stimulating Hormone

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes†

2019 ◽  
Vol 101 (4) ◽  
pp. 719-732 ◽  
Author(s):  
Anna Tetkova ◽  
Andrej Susor ◽  
Michal Kubelka ◽  
Lucie Nemcova ◽  
Denisa Jansova ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Follicle Stimulating Hormone ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Fsh Receptor ◽  
Cytoplasmic Maturation ◽  
Vitro Effect ◽  
Stimulating Hormone

Abstract Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology.

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