Noninvasive Bioluminescence Imaging of Luciferase Expressing Intracranial U87 Xenografts: Correlation with Magnetic Resonance Imaging Determined Tumor Volume and Longitudinal Use in Assessing Tumor Growth and Antiangiogenic Treatment Effect

Small-animal tumor models are essential for developing translational therapeutic strategies in oncology research, with imaging having an increasingly important role. Magnetic resonance imaging (MRI) offers tumor localization, volumetric measurement, and the potential for advanced physiologic imaging but is less well suited to high-throughput studies and has limited capacity to assess early tumor growth. Bioluminescence imaging (BLI) identifies tumors early, monitors tumor growth, and efficiently measures response to therapeutic intervention. Generally, BLI signals have been found to correlate well with magnetic resonance measurements of tumor volume. However, in our studies of small-animal models of malignant brain tumors, we have observed specific instances in which BLI data do not correlate with corresponding MRIs. These observations led us to hypothesize that use of BLI and MRI together, rather than in isolation, would allow more effective and efficient measures of tumor growth in preclinical studies. Herein we describe combining BLI and MRI studies to characterize tumor growth in a mouse model of glioblastoma. The results led us to suggest a cost-effective, multimodality strategy for selecting cohorts of animals with similar tumor growth patterns that improves the accuracy of longitudinal in vivo measurements of tumor growth and treatment response in preclinical therapeutic studies.

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Tumor growth analysis by magnetic resonance imaging of the C6 glioblastoma model with prospects for the assessment of magnetohyperthermia therapy

Einstein (São Paulo) ◽

10.1590/s1679-45082012000100004 ◽

2012 ◽

Vol 10 (1) ◽

pp. 11-15 ◽

Cited By ~ 6

Author(s):

André César da Silva ◽

Francisco Romero Cabral ◽

Javier Bustamante Mamani ◽

Jackeline Moraes Malheiros ◽

Roberson Saraiva Polli ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Tumor Growth ◽

Wistar Rats ◽

Culture Medium ◽

Tumor Volume ◽

Magnetic Hyperthermia ◽

Resonance Imaging ◽

Hyperthermia Therapy ◽

Spin Echo Sequence

OBJECTIVE: The objective was to establish a pattern of tumor growth of the C6 model of glioblastoma multiform in Wistar rats via magnetic resonance imaging (MRI) for the subsequent verification of tumor volume reduction due to magnetic hyperthermia therapy. METHODS: Young male Wistar rats weighing between 250 and 300 g were used for the C6 model. After the rates were anesthetized (55 mg/kg ketamine and 11 mg/kg xylazine), C6 lineage tumorigenic cells suspended in culture medium (10(5) cells in 10 µl) were stereotaxically injected into the right frontal cortex (bregma coordinates: 2.0 mm anteroposterior, 3.0 mm laterolateral, and 2.5 mm depth) of the rats using a Hamilton syringe. For the control group, the rats were injected with culture medium without cells. MRI scans were performed at 14, 21, and 28 d after the injection using a 2.0 T MRI scanner (Bruker BioSpec, Germany). The animals were anesthetized with 55 mg/kg ketamine and 11 mg/kg xylazine before being examined. Coronal multilayers were acquired using a standard spin echo sequence with the following parameters: repetition/echo time = 4.000 ms/67.1 ms, field of view = 3.50, matrix = 192, slice thickness = 0.4 mm, and slice separation = 0 mm. RESULTS: The MRI analysis enabled a clear visualization of the tumor mass, and it was possible to establish the tumor volume parameters on the various days that were examined. The volume at 14 d after induction was 13.7 ± 2.5 mm³. On days 21 and 28, the tumor volumes were 31.7 ± 6.5 mm³ and 122.1 ± 11.8 mm³, respectively. CONCLUSION: These results demonstrated that it is possible to evaluate the C6 model tumor volume in rats, which will allow for the future implementation and verification of magnetic hyperthermia therapy.

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Validation of a combined ultrasound and bioluminescence imaging system with magnetic resonance imaging in orthotopic pancreatic murine tumors

Scientific Reports ◽

10.1038/s41598-021-03684-z ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Juan D. Rojas ◽

Jordan B. Joiner ◽

Brian Velasco ◽

Kathlyne Jayne B. Bautista ◽

Adam M. Aji ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Tumor Volume ◽

Bioluminescence Imaging ◽

Imaging System ◽

Cancer Therapeutics ◽

Detection Sensitivity ◽

Photon Flux ◽

Resonance Imaging ◽

Tumor Models

AbstractPreclinical mouse solid tumor models are widely used to evaluate efficacy of novel cancer therapeutics. Recent reports have highlighted the need for utilizing orthotopic implantation to represent clinical disease more accurately, however the deep tissue location of these tumors makes longitudinal assessment challenging without the use of imaging techniques. The purpose of this study was to evaluate the performance of a new multi-modality high-throughput in vivo imaging system that combines bioluminescence imaging (BLI) with robotic, hands-free ultrasound (US) for evaluating orthotopic mouse models. Long utilized in cancer research as independent modalities, we hypothesized that the combination of BLI and US would offer complementary advantages of detection sensitivity and quantification accuracy, while mitigating individual technological weaknesses. Bioluminescent pancreatic tumor cells were injected into the pancreas tail of C57BL/6 mice and imaged weekly with the combination system and magnetic resonance imaging (MRI) to serve as a gold standard. BLI photon flux was quantified to assess tumor activity and distribution, and US and MRI datasets were manually segmented for gross tumor volume. Robotic US and MRI demonstrated a strong agreement (R2 = 0.94) for tumor volume measurement. BLI showed a weak overall agreement with MRI (R2 = 0.21), however, it offered the greatest sensitivity to detecting the presence of tumors. We conclude that combining BLI with robotic US offers an efficient screening tool for orthotopic tumor models.

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Quantification of Glioma Removal by Intraoperative High-Field Magnetic Resonance Imaging: An Update

Neurosurgery ◽

10.1227/neu.0b013e318225ea6b ◽

2011 ◽

Vol 69 (4) ◽

pp. 852-863 ◽

Cited By ~ 54

Author(s):

Daniela Kuhnt ◽

Oliver Ganslandt ◽

Sven-Martin Schlaffer ◽

Michael Buchfelder ◽

Christopher Nimsky

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

High Field ◽

Tumor Volume ◽

Tumor Resection ◽

World Health ◽

Incomplete Resection ◽

Resonance Imaging ◽

Glioma Surgery ◽

Who Grade

Abstract BACKGROUND: The beneficial role of the extent of resection (EOR) in glioma surgery in correlation to increased survival remains controversial. However, common literature favors maximum EOR with preservation of neurological function, which is shown to be associated with a significantly improved outcome. OBJECTIVE: In order to obtain a maximum EOR, it was examined whether high-field intraoperative magnetic resonance imaging (iMRI) combined with multimodal navigation contributes to a significantly improved EOR in glioma surgery. METHODS: Two hundred ninety-three glioma patients underwent craniotomy and tumor resection with the aid of intraoperative 1.5 T MRI and integrated multimodal navigation. In cases of remnant tumor, an update of navigation was performed with intraoperative images. Tumor volume was quantified pre- and intraoperatively by segmentation of T2 abnormality in low-grade and contrast enhancement in high-grade gliomas. RESULTS: In 25.9% of all cases examined, additional tumor mass was removed as a result of iMRI. This led to complete tumor resection in 20 cases, increasing the rate of gross-total removal from 31.7% to 38.6%. In 56 patients, additional but incomplete resection was performed because of the close location to eloquent brain areas. Volumetric analysis showed a significantly (P < .01) reduced mean percentage of tumor volume following additional further resection after iMRI from 33.5% ± 25.1% to 14.7% ± 23.3% (World Health Organization [WHO] grade I, 32.8% ± 21.9% to 6.1% ± 18.8%; WHO grade II, 24.4% ± 25.1% to 10.8% ± 11.0%; WHO grade III, 35.1% ± 27.3% to 24.8% ± 26.3%; WHO grade IV, 34.2% ± 23.7% to 1.2% ± 16.2%). CONCLUSION: MRI in conjunction with multimodal navigation and an intraoperative updating procedure enlarges tumor-volume reduction in glioma surgery significantly without higher postoperative morbidity.

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Volumetric Analysis Using Low-Field Intraoperative Magnetic Resonance Imaging for 168 Newly Diagnosed Supratentorial Glioblastomas: Effects of Extent of Resection and Residual Tumor Volume on Survival and Recurrence

World Neurosurgery ◽

10.1016/j.wneu.2016.10.109 ◽

2017 ◽

Vol 98 ◽

pp. 73-80 ◽

Cited By ~ 19

Author(s):

Atsushi Fukui ◽

Yoshihiro Muragaki ◽

Taiichi Saito ◽

Takashi Maruyama ◽

Masayuki Nitta ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Tumor Volume ◽

Residual Tumor ◽

Extent Of Resection ◽

Volumetric Analysis ◽

Newly Diagnosed ◽

Resonance Imaging ◽

Intraoperative Magnetic Resonance Imaging ◽

Low Field

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Pre- and Post-Operative Volume Estimation of Medulloblastoma Using Magnetic Resonance Imaging: A Stereological Study

Iranian Journal of Radiology ◽

10.5812/iranjradiol.97047 ◽

2020 ◽

Vol 17 (3) ◽

Author(s):

Farzaneh Dehghani ◽

Seyede Fatemeh Hosseini ◽

Reza Jalli ◽

Fatemeh Karimi ◽

Narges Sotoudeh ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Tumor Volume ◽

Tumor Regression ◽

Normal Subjects ◽

Volume Estimation ◽

Unbiased Estimation ◽

Cerebellar Tumor ◽

Resonance Imaging ◽

Counting Approach

Background: Brain tumors are among the most lethal and devastating cancers. Medulloblastoma tumor is a common solid brain malignancy, arising in the posterior fossa. Stereological methods are used in combination with magnetic resonance imaging (MRI) to obtain unbiased estimation of the total volume of the structure of interest. Objectives: In the current study, we evaluated cerebellar tumor volume pre- and post-operative in patients suffering from meduloblastoma tumor using MRI and Cavalieri method. Patients and Methods: The study was performed on 16 subjects consisting of two groups of eight people including patient and control groups. Slide direction were in both sagittal and axial planes. Pre- and post-operative volume of medulloblastoma tumor were determined on MR scanning images using the point-counting approach of Cavalieri method. Results: The post-operative tumor volume in both sagittal (P = 0.028) and axial (P = 0.046) MR images was statistically reduced by 90% compared to the pre-operative volume. Moreover, the pre-operative volume of the cerebellum in patients increased by 38% in patients as compared to the normal subjects (P = 0.028). Conclusion: It could be concluded that MRI-estimated tumor-volume might be useful in evaluating the efficiency of surgical treatment and prognostication of tumor regression rate.

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