scholarly journals Measuring Brain Tumor Growth: Combined Bioluminescence Imaging–Magnetic Resonance Imaging Strategy

2009 ◽  
Vol 8 (5) ◽  
pp. 7290.2009.00023 ◽  
Author(s):  
Sarah C. Jost ◽  
Lynne Collins ◽  
Sarah Travers ◽  
David Piwnica-Worms ◽  
Joel R. Garbow
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Tumor Growth ◽  
Bioluminescence Imaging ◽  
Small Animal ◽  
Cost Effective ◽  
Growth Patterns ◽  
Resonance Imaging ◽  
Oncology Research

Small-animal tumor models are essential for developing translational therapeutic strategies in oncology research, with imaging having an increasingly important role. Magnetic resonance imaging (MRI) offers tumor localization, volumetric measurement, and the potential for advanced physiologic imaging but is less well suited to high-throughput studies and has limited capacity to assess early tumor growth. Bioluminescence imaging (BLI) identifies tumors early, monitors tumor growth, and efficiently measures response to therapeutic intervention. Generally, BLI signals have been found to correlate well with magnetic resonance measurements of tumor volume. However, in our studies of small-animal models of malignant brain tumors, we have observed specific instances in which BLI data do not correlate with corresponding MRIs. These observations led us to hypothesize that use of BLI and MRI together, rather than in isolation, would allow more effective and efficient measures of tumor growth in preclinical studies. Herein we describe combining BLI and MRI studies to characterize tumor growth in a mouse model of glioblastoma. The results led us to suggest a cost-effective, multimodality strategy for selecting cohorts of animals with similar tumor growth patterns that improves the accuracy of longitudinal in vivo measurements of tumor growth and treatment response in preclinical therapeutic studies.

scholarly journals Magnetic Resonance Imaging in Animal Models of Alzheimer’s Disease Amyloidosis

2021 ◽  
Author(s):  
Ruiqing Ni
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Animal Models ◽  
Amyloid Beta ◽  
High Field ◽  
Diffusion Tensor ◽  
Small Animal ◽  
Resonance Imaging ◽  
Magnetic Resonance Imaging Mri

Amyloid-beta plays an important role in the pathogenesis of Alzheimer’s disease. Aberrant amyloid-beta and tau accumulation induce neuroinflammation, cerebrovascular alterations, synaptic deficits, functional deficits, and neurodegeneration, leading to cognitive impairment. Animal models recapitulating the amyloid-beta pathology such as transgenic, knock-in mouse and rat models have facilitated the understanding of disease mechanisms and development of therapeutics targeting at amyloid-beta. There is a rapid advance in high-field MR in small animals. Versatile high-field magnetic resonance imaging (MRI) sequences such as diffusion tensor imaging, arterial spin labelling, resting-state functional MRI, anatomical MRI, MR spectroscopy as well as contrast agents have been developed for the applications in animal models. These tools have enabled high-resolution in vivo structural, functional, and molecular readouts with a whole brain field-of-view. MRI have been utilized to visualize non-invasively the amyloid-beta deposits, synaptic deficits, regional brain atrophy, impairment in white matter integrity, functional connectivity, cerebrovascular and glymphatic system in animal models of amyloidosis. Many of the readouts are translational in clinical MRI in the brain of patients with Alzheimer’s disease. In this review, we summarize the recent advance of using MRI for visualizing the pathophysiology in amyloidosis animal model. We discuss the outstanding challenges in brain imaging using MRI in small animal and propose future outlook in visualizing amyloid-beta-related alterations in brain of animal models.

scholarly journals Magnetic Resonance Imaging for Translational Research in Oncology

2019 ◽  
Vol 8 (11) ◽  
pp. 1883 ◽  
Author(s):  
Maria Felicia Fiordelisi ◽  
Carlo Cavaliere ◽  
Luigi Auletta ◽  
Luca Basso ◽  
Marco Salvatore
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Small Animal ◽  
Laboratory Animals ◽  
Resonance Imaging ◽  
Preclinical Research ◽  
Ongoing Research ◽  
Wide Range

The translation of results from the preclinical to the clinical setting is often anything other than straightforward. Indeed, ideas and even very intriguing results obtained at all levels of preclinical research, i.e., in vitro, on animal models, or even in clinical trials, often require much effort to validate, and sometimes, even useful data are lost or are demonstrated to be inapplicable in the clinic. In vivo, small-animal, preclinical imaging uses almost the same technologies in terms of hardware and software settings as for human patients, and hence, might result in a more rapid translation. In this perspective, magnetic resonance imaging might be the most translatable technique, since only in rare cases does it require the use of contrast agents, and when not, sequences developed in the lab can be readily applied to patients, thanks to their non-invasiveness. The wide range of sequences can give much useful information on the anatomy and pathophysiology of oncologic lesions in different body districts. This review aims to underline the versatility of this imaging technique and its various approaches, reporting the latest preclinical studies on thyroid, breast, and prostate cancers, both on small laboratory animals and on human patients, according to our previous and ongoing research lines.

scholarly journals Development of the 1.2 T~1.5 T Permanent Magnetic Resonance Imaging Device and Its Application for Mouse Imaging

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Guangxin Wang ◽  
Huantong Xie ◽  
Shulian Hou ◽  
Wei Chen ◽  
Qiang Zhao ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Permanent Magnet ◽  
Three Dimensional ◽  
Small Animal ◽  
Cost Effective ◽  
Main Magnetic Field ◽  
Resonance Imaging ◽  
Imaging Device ◽  
Mouse Imaging

By improving the main magnet, gradient, and RF coils design technology, manufacturing methods, and inventing new magnetic resonance imaging (MRI) special alloy, a cost-effective and small animal specific permanent magnet-type three-dimensional magnetic resonance imager was developed. The main magnetic field strength of magnetic resonance imager with independent intellectual property rights is 1.2~1.5 T. To demonstrate its effectiveness and validate the mouse imaging experiments in different directions, we compared the images obtained by small animal specific permanent magnet-type three-dimensional magnetic resonance imager with that obtained by using superconductor magnetic resonance imager for clinical diagnosis.

scholarly journals Value CMR: Towards a Comprehensive, Rapid, Cost-Effective Cardiovascular Magnetic Resonance Imaging

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
El-Sayed H. Ibrahim ◽  
Luba Frank ◽  
Dhiraj Baruah ◽  
V. Emre Arpinar ◽  
Andrew S. Nencka ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Tissue Characterization ◽  
T2 Mapping ◽  
Cost Effective ◽  
Cine Imaging ◽  
Resonance Imaging ◽  
4D Flow ◽  
Scan Time

Cardiac magnetic resonance imaging (CMR) is considered the gold standard for measuring cardiac function. Further, in a single CMR exam, information about cardiac structure, tissue composition, and blood flow could be obtained. Nevertheless, CMR is underutilized due to long scanning times, the need for multiple breath-holds, use of a contrast agent, and relatively high cost. In this work, we propose a rapid, comprehensive, contrast-free CMR exam that does not require repeated breath-holds, based on recent developments in imaging sequences. Time-consuming conventional sequences have been replaced by advanced sequences in the proposed CMR exam. Specifically, conventional 2D cine and phase-contrast (PC) sequences have been replaced by optimized 3D-cine and 4D-flow sequences, respectively. Furthermore, conventional myocardial tagging has been replaced by fast strain-encoding (SENC) imaging. Finally, T1 and T2 mapping sequences are included in the proposed exam, which allows for myocardial tissue characterization. The proposed rapid exam has been tested in vivo. The proposed exam reduced the scan time from >1 hour with conventional sequences to <20 minutes. Corresponding cardiovascular measurements from the proposed rapid CMR exam showed good agreement with those from conventional sequences and showed that they can differentiate between healthy volunteers and patients. Compared to 2D cine imaging that requires 12-16 separate breath-holds, the implemented 3D-cine sequence allows for whole heart coverage in 1-2 breath-holds. The 4D-flow sequence allows for whole-chest coverage in less than 10 minutes. Finally, SENC imaging reduces scan time to only one slice per heartbeat. In conclusion, the proposed rapid, contrast-free, and comprehensive cardiovascular exam does not require repeated breath-holds or to be supervised by a cardiac imager. These improvements make it tolerable by patients and would help improve cost effectiveness of CMR and increase its adoption in clinical practice.

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