scholarly journals Ballchen is required for self-renewal of germline stem cells in Drosophila melanogaster

Biology Open ◽  
2014 ◽  
Vol 3 (6) ◽  
pp. 510-521 ◽  
Author(s):  
B. Herzig ◽  
T. A. Yakulov ◽  
K. Klinge ◽  
U. Gunesdogan ◽  
H. Jackle ◽  
...  
Keyword(s):  
Stem Cells ◽  
Drosophila Melanogaster ◽  
Germline Stem Cells ◽  
Self Renewal

scholarly journals The miRNA Pathway Intrinsically Controls Self-Renewal of Drosophila Germline Stem Cells

2007 ◽  
Vol 17 (6) ◽  
pp. 533-538 ◽  
Author(s):  
Joseph K. Park ◽  
Xiang Liu ◽  
Tamara J. Strauss ◽  
Dennis M. McKearin ◽  
Qinghua Liu
Keyword(s):  
Stem Cells ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Mirna Pathway

scholarly journals Endocrine regulation of female germline stem cells in the fruit fly Drosophila melanogaster

2019 ◽  
Vol 31 ◽  
pp. 14-19 ◽  
Author(s):  
Yuto Yoshinari ◽  
Yoshitomo Kurogi ◽  
Tomotsune Ameku ◽  
Ryusuke Niwa
Keyword(s):  
Stem Cells ◽  
Drosophila Melanogaster ◽  
Fruit Fly ◽  
Endocrine Regulation ◽  
Germline Stem Cells ◽  
Female Germline

scholarly journals Functional Assessment of Self-Renewal Activity of Male Germline Stem Cells Following Cytotoxic Damage and Serial Transplantation1

2003 ◽  
Vol 68 (5) ◽  
pp. 1801-1807 ◽  
Author(s):  
Mito Kanatsu-Shinohara ◽  
Shinya Toyokuni ◽  
Takeshi Morimoto ◽  
Shigeyuki Matsui ◽  
Tasuku Honjo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Assessment ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Male Germline ◽  
Male Germline Stem Cells

scholarly journals Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Cuie Chen ◽  
Ryan Cummings ◽  
Aghapi Mordovanakis ◽  
Alan J Hunt ◽  
Michael Mayer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Cell Fate ◽  
Cell Polarity ◽  
Adult Stem Cells ◽  
Asymmetric Cell Division ◽  
Cytokine Receptor ◽  
Spindle Orientation ◽  
Germline Stem Cells ◽  
Self Renewal

Asymmetric stem cell division is a critical mechanism for balancing self-renewal and differentiation. Adult stem cells often orient their mitotic spindle to place one daughter inside the niche and the other outside of it to achieve asymmetric division. It remains unknown whether and how the niche may direct division orientation. Here we discover a novel and evolutionary conserved mechanism that couples cell polarity to cell fate. We show that the cytokine receptor homolog Dome, acting downstream of the niche-derived ligand Upd, directly binds to the microtubule-binding protein Eb1 to regulate spindle orientation in Drosophila male germline stem cells (GSCs). Dome’s role in spindle orientation is entirely separable from its known function in self-renewal mediated by the JAK-STAT pathway. We propose that integration of two functions (cell polarity and fate) in a single receptor is a key mechanism to ensure an asymmetric outcome following cell division.

scholarly journals Centrosome misorientation mediates slowing of the cell cycle under limited nutrient conditions in Drosophila male germline stem cells

2012 ◽  
Vol 23 (8) ◽  
pp. 1524-1532 ◽  
Author(s):  
Therese M. Roth ◽  
C.-Y. Ason Chiang ◽  
Mayu Inaba ◽  
Hebao Yuan ◽  
Viktoria Salzmann ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Stem Cell Regulation ◽  
Male Germline ◽  
Male Germline Stem Cells ◽  
Nutrient Conditions

Drosophila male germline stem cells (GSCs) divide asymmetrically, balancing self-renewal and differentiation. Although asymmetric stem cell division balances between self-renewal and differentiation, it does not dictate how frequently differentiating cells must be produced. In male GSCs, asymmetric GSC division is achieved by stereotyped positioning of the centrosome with respect to the stem cell niche. Recently we showed that the centrosome orientation checkpoint monitors the correct centrosome orientation to ensure an asymmetric outcome of the GSC division. When GSC centrosomes are not correctly oriented with respect to the niche, GSC cell cycle is arrested/delayed until the correct centrosome orientation is reacquired. Here we show that induction of centrosome misorientation upon culture in poor nutrient conditions mediates slowing of GSC cell proliferation via activation of the centrosome orientation checkpoint. Consistently, inactivation of the centrosome orientation checkpoint leads to lack of cell cycle slowdown even under poor nutrient conditions. We propose that centrosome misorientation serves as a mediator that transduces nutrient information into stem cell proliferation, providing a previously unappreciated mechanism of stem cell regulation in response to nutrient conditions.

Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis

2011 ◽  
Vol 112 (4) ◽  
pp. 1009-1021 ◽  
Author(s):  
Shanshan Zhang ◽  
Junwei Sun ◽  
Shaohui Pan ◽  
Haijing Zhu ◽  
Long Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vitamin A ◽  
Adult Mouse ◽  
Mouse Testis ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Male Germline ◽  
Male Germline Stem Cells

AKT3 Is a Pivotal Molecule of Cadherin-22 and GDNF Family Receptor-α1 Signal Pathways Regulating Self-Renewal in Female Germline Stem Cells

Stem Cells ◽  
2019 ◽  
Vol 37 (8) ◽  
pp. 1095-1107 ◽  
Author(s):  
Xiaoyu Zhang ◽  
Rui Wei ◽  
Yizhuo Sun ◽  
Qin Xia ◽  
Wenhai Xie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Signal Pathways ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Female Germline

The male stem cell niche of Drosophila melanogaster: Interactions between the germline stem cells and the hub

2019 ◽  
Vol 383 (1) ◽  
pp. 111489
Author(s):  
Veronica Persico ◽  
Giuliano Callaini ◽  
Maria Giovanna Riparbelli
Keyword(s):  
Stem Cells ◽  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Germline Stem Cells ◽  
Cell Niche

scholarly journals Enrichment of Female Germline Stem Cells from Mouse Ovaries Using the Differential Adhesion Method

2018 ◽  
Vol 46 (5) ◽  
pp. 2114-2126 ◽  
Author(s):  
Meng Wu ◽  
Jiaqiang Xiong ◽  
Lingwei Ma ◽  
Zhiyong Lu ◽  
Xian Qin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Akt Pathway ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Differential Adhesion ◽  
Proliferation And Differentiation ◽  
Phosphoinositide 3 Kinase ◽  
Female Germline

Background/Aims: The isolation and establishment of female germline stem cells (FGSCs) is controversial because of questions regarding the reliability and stability of the isolation method using antibody targeting mouse vasa homologue (MVH), and the molecular mechanism of FGSCs self-renewal remains unclear. Thus, there needs to be a simple and reliable method for sorting FGSCs to study them. Methods: We applied the differential adhesion method to enrich FGSCs (DA-FGSCs) from mouse ovaries. Through four rounds of purification and 7-9 subsequent passages, DA-FGSC lines were established. In addition, we assessed the role of the phosphoinositide-3 kinase (PI3K)-AKT pathway in regulating FGSC self-renewal. Results: The obtained DA-FGSCs spontaneously differentiated into oocyte-like cells in vitro and formed functional eggs in vivo that were fertilized and produced healthy offspring. AKT was rapidly phosphorylated when the proliferation rate of FGSCs increased after 10 passages, and the addition of a chemical PI3K inhibitor prevented FGSCs self-renewal. Furthermore, over-expression of AKT-induced proliferation and differentiation of FGSCs, c-Myc, Oct-4 and Gdf-9 levels were increased. Conclusions: The differential adhesion method provides a more feasible approach and is an easier procedure to establish FGSC lines than traditional methods. The AKT pathway plays an important role in regulation of the proliferation and maintenance of FGSCs. These findings could help promote stem cell studies and provide a better understanding of causes of ovarian infertility, thereby providing potential treatments for infertility.

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