TRICOT encodes an AMP1-related carboxypeptidase that regulates root nodule development and shoot apical meristem maintenance in Lotus japonicus

Development ◽  
2012 ◽  
Vol 140 (2) ◽  
pp. 353-361 ◽  
Author(s):  
T. Suzaki ◽  
C. S. Kim ◽  
N. Takeda ◽  
K. Szczyglowski ◽  
M. Kawaguchi
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Meristem Maintenance

scholarly journals Spatiotemporal Sequestration of miR165/166 by Arabidopsis Argonaute10 Promotes Shoot Apical Meristem Maintenance

Cell Reports ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. 1819-1827 ◽  
Author(s):  
Yuyi Zhou ◽  
Minami Honda ◽  
Hongliang Zhu ◽  
Zhonghui Zhang ◽  
Xinwei Guo ◽  
...  
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Maintenance

scholarly journals Control of Rice Embryo Development, Shoot Apical Meristem Maintenance, and Grain Yield by a Novel Cytochrome P450

2013 ◽  
Vol 6 (6) ◽  
pp. 1945-1960 ◽  
Author(s):  
Weibing Yang ◽  
Mingjun Gao ◽  
Xin Yin ◽  
Jiyun Liu ◽  
Yonghan Xu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Grain Yield ◽  
Embryo Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Maintenance ◽  
Rice Embryo

scholarly journals Expression Analysis of PIN Genes in Root Tips and Nodules of Lotus japonicus

2019 ◽  
Vol 20 (2) ◽  
pp. 235 ◽  
Author(s):  
Izabela Sańko-Sawczenko ◽  
Dominika Dmitruk ◽  
Barbara Łotocka ◽  
Elżbieta Różańska ◽  
Weronika Czarnocka
Keyword(s):  
Root Nodule ◽  
Root Nodules ◽  
Quantitative Polymerase Chain Reaction ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Vascular Bundles ◽  
Root Tips ◽  
Gus Reporter ◽  
Gene Assay ◽  
Development And Differentiation

Auxins are postulated to be one of the pivotal factors in nodulation. However, their transporters in Lotus japonicus, the model species for the study of the development of determinate-type root nodules, have been scarcely described so far, and thus their role in nodulation has remained unknown. Our research is the first focusing on polar auxin transporters in L. japonicus. We analyzed and compared expression of PINs in 20 days post rhizobial inoculation (dpi) and 54 dpi root nodules of L. japonicus by real-time quantitative polymerase chain reaction (qPCR) along with the histochemical β-glucuronidase (GUS) reporter gene assay in transgenic hairy roots. The results indicate that LjPINs are essential during root nodule development since they are predominantly expressed in the primordia and young, developing nodules. However, along with differentiation, expression levels of several PINs decreased and occurred particularly in the nodule vascular bundles, especially in connection with the root’s stele. Moreover, our study demonstrated the importance of both polar auxin transport and auxin intracellular homeostasis during L. japonicus root nodule development and differentiation.

scholarly journals L1 Division and Differentiation Patterns Influence Shoot Apical Meristem Maintenance

2006 ◽  
Vol 141 (4) ◽  
pp. 1349-1362 ◽  
Author(s):  
Sharon Kessler ◽  
Brad Townsley ◽  
Neelima Sinha
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Meristem Maintenance

scholarly journals FAR-RED ELONGATED HYPOCOTYL3 activates SEPALLATA2 but inhibits CLAVATA3 to regulate meristem determinacy and maintenance in Arabidopsis

2016 ◽  
Vol 113 (33) ◽  
pp. 9375-9380 ◽  
Author(s):  
Dongming Li ◽  
Xing Fu ◽  
Lin Guo ◽  
Zhigang Huang ◽  
Yongpeng Li ◽  
...  
Keyword(s):  
Flower Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Target Genes ◽  
Developmental Stages ◽  
Binding Motif ◽  
Phytochrome B ◽  
Helix Loop Helix ◽  
Before And After ◽  
Meristem Maintenance

Plant meristems are responsible for the generation of all plant tissues and organs. Here we show that the transcription factor (TF) FAR-RED ELONGATED HYPOCOTYL3 (FHY3) plays an important role in both floral meristem (FM) determinacy and shoot apical meristem maintenance in Arabidopsis, in addition to its well-known multifaceted roles in plant growth and development during the vegetative stage. Through genetic analyses, we show that WUSCHEL (WUS) and CLAVATA3 (CLV3), two central players in the establishment and maintenance of meristems, are epistatic to FHY3. Using genome-wide ChIP-seq and RNA-seq data, we identify hundreds of FHY3 target genes in flowers and find that FHY3 mainly acts as a transcriptional repressor in flower development, in contrast to its transcriptional activator role in seedlings. Binding motif-enrichment analyses indicate that FHY3 may coregulate flower development with three flower-specific MADS-domain TFs and four basic helix–loop–helix TFs that are involved in photomorphogenesis. We further demonstrate that CLV3, SEPALLATA1 (SEP1), and SEP2 are FHY3 target genes. In shoot apical meristem, FHY3 directly represses CLV3, which consequently regulates WUS to maintain the stem cell pool. Intriguingly, CLV3 expression did not change significantly in fhy3 and phytochrome B mutants before and after light treatment, indicating that FHY3 and phytochrome B are involved in light-regulated meristem activity. In FM, FHY3 directly represses CLV3, but activates SEP2, to ultimately promote FM determinacy. Taken together, our results reveal insights into the mechanisms of meristem maintenance and determinacy, and illustrate how the roles of a single TF may vary in different organs and developmental stages.

scholarly journals Grass Meristems I: Shoot Apical Meristem Maintenance, Axillary Meristem Determinacy and the Floral Transition

2013 ◽  
Vol 54 (3) ◽  
pp. 302-312 ◽  
Author(s):  
Michael Pautler ◽  
Wakana Tanaka ◽  
Hiro-Yuki Hirano ◽  
David Jackson
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Floral Transition ◽  
Axillary Meristem ◽  
Meristem Maintenance

Flower meristem maintenance by TILLERS ABSENT 1 is essential for ovule development in rice

Development ◽  
2021 ◽  
Vol 148 (24) ◽  
Author(s):  
Wakana Tanaka ◽  
Suzuha Ohmori ◽  
Naoto Kawakami ◽  
Hiro-Yuki Hirano
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Flower Development ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Stem Cell Marker ◽  
Ovule Development ◽  
Flower Meristem ◽  
Ovule Formation ◽  
Meristem Maintenance

ABSTRACT Plant development depends on the activity of pluripotent stem cells in meristems, such as the shoot apical meristem and the flower meristem. In Arabidopsis thaliana, WUSCHEL (WUS) is essential for stem cell homeostasis in meristems and integument differentiation in ovule development. In rice (Oryza sativa), the WUS ortholog TILLERS ABSENT 1 (TAB1) promotes stem cell fate in axillary meristem development, but its function is unrelated to shoot apical meristem maintenance in vegetative development. In this study, we examined the role of TAB1 in flower development. The ovule, which originates directly from the flower meristem, failed to differentiate in tab1 mutants, suggesting that TAB1 is required for ovule formation. Expression of a stem cell marker was completely absent in the flower meristem at the ovule initiation stage, indicating that TAB1 is essential for stem cell maintenance in the ‘final’ flower meristem. The ovule defect in tab1 was partially rescued by floral organ number 2 mutation, which causes overproliferation of stem cells. Collectively, it is likely that TAB1 promotes ovule formation by maintaining stem cells at a later stage of flower development.

scholarly journals Spontaneous Root-Nodule Formation in the Model Legume Lotus japonicus: A Novel Class of Mutants Nodulates in the Absence of Rhizobia

2006 ◽  
Vol 19 (4) ◽  
pp. 373-382 ◽  
Author(s):  
Leïla Tirichine ◽  
Euan K. James ◽  
Niels Sandal ◽  
Jens Stougaard
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Nodule Formation ◽  
Wild Type ◽  
Model Legume ◽  
Isolation And Characterization ◽  
Mutant Lines ◽  
Spontaneous Nodules

Root-nodule development in legumes is an inducible developmental process initially triggered by perception of lipochitin-oligosaccharide signals secreted by the bacterial microsymbiont. In nature, rhizobial colonization and invasion of the legume root is therefore a prerequisite for formation of nitrogen-fixing root nodules. Here, we report isolation and characterization of chemically induced spontaneously nodulating mutants in a model legume amenable to molecular genetics. Six mutant lines of Lotus japonicus were identified in a screen for spontaneous nodule development under axenic conditions, i.e., in the absence of rhizobia. Spontaneous nodules do not contain rhizobia, bacteroids, or infection threads. Phenotypically, they resemble ineffective white nodules formed by some bacterial mutants on wild-type plants or certain plant mutants inoculated with wild-type Mesorhizobium loti. Spontaneous nodules formed on mutant lines show the ontogeny and characteristic histological features described for rhizobia-induced nodules on wild-type plants. Physiological responses to nitrate and ethylene are also maintained, as elevated levels inhibit spontaneous nodulation. Activation of the nodule developmental program in spontaneous nodules was shown for the early nodulin genes Enod2 and Nin, which are both upregulated in spontaneous nodules as well as in rhizobial nodules. Both monogenic recessive and dominant spontaneous nodule formation (snf) mutations were isolated in this mutant screen, and map positions were determined for three loci. We suggest that future molecular characterization of these mutants will identify key plant determinants involved in regulating nodulation and provide new insight into plant organ development.

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