scholarly journals Quantitative imaging of cell dynamics in mouse embryos using light-sheet microscopy

2014 ◽  
Vol 127 (23) ◽  
pp. e1-e1
Author(s):  
R. S. Udan ◽  
V. G. Piazza ◽  
C.-w. Hsu ◽  
A.-K. Hadjantonakis ◽  
M. E. Dickinson
Keyword(s):  
Quantitative Imaging ◽  
Light Sheet ◽  
Mouse Embryos ◽  
Cell Dynamics ◽  
Light Sheet Microscopy

scholarly journals Quantitative imaging of cell dynamics in mouse embryos using light-sheet microscopy

Development ◽  
2014 ◽  
Vol 141 (22) ◽  
pp. 4406-4414 ◽  
Author(s):  
R. S. Udan ◽  
V. G. Piazza ◽  
C.-w. Hsu ◽  
A.-K. Hadjantonakis ◽  
M. E. Dickinson
Keyword(s):  
Quantitative Imaging ◽  
Light Sheet ◽  
Mouse Embryos ◽  
Cell Dynamics ◽  
Light Sheet Microscopy

scholarly journals High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Benjamin Schmid ◽  
Gopi Shah ◽  
Nico Scherf ◽  
Michael Weber ◽  
Konstantin Thierbach ◽  
...  
Keyword(s):  
High Speed ◽  
Light Sheet ◽  
Endodermal Cell ◽  
Cell Dynamics ◽  
Light Sheet Microscopy

scholarly journals Erratum: Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools

2014 ◽  
Vol 9 (10) ◽  
pp. 2513-2513 ◽  
Author(s):  
Takehiko Ichikawa ◽  
Kenichi Nakazato ◽  
Philipp J Keller ◽  
Hiroko Kajiura-Kobayashi ◽  
Ernst H K Stelzer ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Light Sheet ◽  
Live Imaging ◽  
Mouse Embryos ◽  
3D Tracking ◽  
Light Sheet Microscopy

scholarly journals Quantitative Imaging of Cellular Morphodynamics and Signaling with Light-Sheet Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 6a-7a
Author(s):  
Reto P. Fiolka ◽  
Kevin M. Dean ◽  
Meghan Driscoll ◽  
Erik Welf ◽  
Gaudenz Danuser
Keyword(s):  
Quantitative Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Light Sheet Theta Microscopy for High-resolution Quantitative Imaging of Large Biological Systems

2017 ◽  
Author(s):  
Bianca Migliori ◽  
Malika S. Datta ◽  
Christophe Dupre ◽  
Mehmet C. Apak ◽  
Shoh Asano ◽  
...  
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Quantitative Imaging ◽  
Biological Systems ◽  
Light Sheet ◽  
Planar Imaging ◽  
Light Sheet Microscopy ◽  
Significant Step ◽  
Resolution Imaging ◽  
And Function

AbstractAdvances in tissue clearing and molecular labelling methods are enabling unprecedented optical access to large intact biological systems. These advances fuel the need for high-speed microscopy approaches to image large samples quantitatively and at high resolution. While Light Sheet Microscopy (LSM), with its high planar imaging speed and low photo-bleaching, can be effective, scaling up to larger imaging volumes has been hindered by the use of orthogonal light-sheet illumination. To address this fundamental limitation, we have developed Light Sheet Theta Microscopy (LSTM), which uniformly illuminates samples from same side as the detection objective, thereby eliminating limits on lateral dimensions without sacrificing imaging resolution, depth and speed. We present detailed characterization of LSTM, and show that this approach achieves rapid high-resolution imaging of large intact samples with superior uniform high-resolution than LSM. LSTM is a significant step in high-resolution quantitative mapping of structure and function of large intact biological systems.

Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools

2014 ◽  
Vol 9 (3) ◽  
pp. 575-585 ◽  
Author(s):  
Takehiko Ichikawa ◽  
Kenichi Nakazato ◽  
Philipp J Keller ◽  
Hiroko Kajiura-Kobayashi ◽  
Ernst H K Stelzer ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Light Sheet ◽  
Live Imaging ◽  
Mouse Embryos ◽  
3D Tracking ◽  
Light Sheet Microscopy

scholarly journals 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yu Shan Huang ◽  
Hui Yu Ku ◽  
Yun Chi Tsai ◽  
Chin Hao Chang ◽  
Sih Hua Pao ◽  
...  
Keyword(s):  
Light Sheet ◽  
Cell Dynamics ◽  
Light Sheet Microscopy

scholarly journals Achromatic terahertz Airy beam generation with dielectric metasurfaces

Nanophotonics ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Qingqing Cheng ◽  
Juncheng Wang ◽  
Ling Ma ◽  
Zhixiong Shen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Biomedical Imaging ◽  
Frequency Dispersion ◽  
Light Sheet ◽  
Photonic Devices ◽  
Self Healing ◽  
Beam Focusing ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Potential Applications ◽  
Airy Beams

AbstractAiry beams exhibit intriguing properties such as nonspreading, self-bending, and self-healing and have attracted considerable recent interest because of their many potential applications in photonics, such as to beam focusing, light-sheet microscopy, and biomedical imaging. However, previous approaches to generate Airy beams using photonic structures have suffered from severe chromatic problems arising from strong frequency dispersion of the scatterers. Here, we design and fabricate a metasurface composed of silicon posts for the frequency range 0.4–0.8 THz in transmission mode, and we experimentally demonstrate achromatic Airy beams exhibiting autofocusing properties. We further show numerically that a generated achromatic Airy-beam-based metalens exhibits self-healing properties that are immune to scattering by particles and that it also possesses a larger depth of focus than a traditional metalens. Our results pave the way to the realization of flat photonic devices for applications to noninvasive biomedical imaging and light-sheet microscopy, and we provide a numerical demonstration of a device protocol.

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